Restriction map for
pFN21AE5322
(Circular) (Six-base) MAPSORT of: pFN21AE5322.seq Check: 1259 from: 1 to: 5497
pFN21AE5322 5497 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2335 2335
Size: 5497
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3307 278
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2091 2840 1937
Size: 154 749 4594
Fragments arranged by size:
4594 749 154
Acc65I G'GTAC_C
Cuts at: 2818 2818
Size: 5497
AclI AA'CG_TT
Cuts at: 1563 3624 3997 1563
Size: 2061 373 3063
Fragments arranged by size:
3063 2061 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 3560 4944 5212 1632
Size: 464 1464 1384 268 1917
Fragments arranged by size:
1917 1464 1384 464 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5300
AflIII A'CryG_T
Cuts at: 1203 2160 4396 5373 1203
Size: 957 2236 977 1327
Fragments arranged by size:
2236 1327 977 957
AgeI A'CCGG_T
Cuts at: 4328 4328
Size: 5497
AhdI GACnn_n'nnGTC
Cuts at: 4226 4226
Size: 5497
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5497
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5497
AlwNI CAG_nnn'CTG
Cuts at: 3240 4812 3240
Size: 1572 3925
ApaLI G'TGCA_C
Cuts at: 3554 4710 3554
Size: 1156 4341
ApoI r'AATT_y
Cuts at: 1493 2806 3032 3184 5293 1493
Size: 1313 226 152 2109 1697
Fragments arranged by size:
2109 1697 1313 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4051 160
Size: 872 3019 1606
Fragments arranged by size:
3019 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
AvaI C'yCGr_G
Cuts at: 1870 1943 2131 2228 2403 2598 2630 2822 1870
Size: 73 188 97 175 195 32 192 4545
Fragments arranged by size:
4545 195 192 188 175 97 73 32
BamHI G'GATC_C
Cuts at: 2562 2827 3194 2562
Size: 265 367 4865
Fragments arranged by size:
4865 367 265
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2818 4273 618
Size: 325 217 627 1031 1455 1842
Fragments arranged by size:
1842 1455 1031 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2229 2574 2654 2816 1391
Size: 838 345 80 162 4072
Fragments arranged by size:
4072 838 345 162 80
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4526
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3688 3722 1903
Size: 34 1751 34 3678
Fragments arranged by size:
3678 1751 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3389 4599 3389
Size: 1210 4287
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5497
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 2295 4108
Size: 107 122 71 631 605 343 280 1813
Cuts at: 4108 136
Size: 1525
Fragments arranged by size:
1813 1525 631 605 343 280 122 107
71
BglII A'GATC_T
Cuts at: 5492 5492
Size: 5497
BlpI GC'TnA_GC
Cuts at: 2213 2914 2213
Size: 701 4796
Bme1580I G_kGCm'C
Cuts at: 1790 3558 4714 1790
Size: 1768 1156 2573
Fragments arranged by size:
2573 1768 1156
BmgBI CAC'GTC
Cuts at: 1318 2086 2161 1318
Size: 768 75 4654
Fragments arranged by size:
4654 768 75
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3228 4186 442
Size: 416 53 83 399 24 1811 958 1753
Fragments arranged by size:
1811 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 2378 2540 4157 1239
Size: 794 345 162 1617 2579
Fragments arranged by size:
2579 1617 794 345 162
Bpu10I CC'TnA_GC
Cuts at: 2223 2223
Size: 5497
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3622 4487 4785 3622
Size: 865 298 4334
Fragments arranged by size:
4334 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2112 4160 882
Size: 617 613 2048 2219
Fragments arranged by size:
2219 2048 617 613
BsaAI yAC'GTr
Cuts at: 493 1204 3288 493
Size: 711 2084 2702
Fragments arranged by size:
2702 2084 711
BsaBI GATnn'nnATC
Cuts at: 5491 5491
Size: 5497
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2298 3304 3686 4321
Size: 53 83 186 936 765 1006 382 635
Cuts at: 4321 275
Size: 1451
Fragments arranged by size:
1451 1006 936 765 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2559 2693 3930 4328 4602 4749 5160 2559
Size: 134 1237 398 274 147 411 2896
Fragments arranged by size:
2896 1237 411 398 274 147 134
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2579 2609 1178
Size: 30 165 30 1176 30 4066
Fragments arranged by size:
4066 1176 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2421 2476 2662 1186
Size: 1235 55 186 4021
Fragments arranged by size:
4021 1235 186 55
BseYI C'CCAG_C
Cuts at: 2425 2620 4700 5196 2425
Size: 195 2080 496 2726
Fragments arranged by size:
2726 2080 496 195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2357 1163
Size: 573 621 4303
Fragments arranged by size:
4303 621 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2473 2947 3708 4736 1540
Size: 275 186 472 474 761 1028 2301
Fragments arranged by size:
2301 1028 761 474 472 275 186
BsiHKAI G_wGCw'C
Cuts at: 2214 2229 2607 3558 3643 4714 2214
Size: 15 378 951 85 1071 2997
Fragments arranged by size:
2997 1071 951 378 85 15
BsmI GAATG_Cn'
Cuts at: 3008 3101 3008
Size: 93 5404
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2214 2229 2574 2607 2654 2816 3558
Size: 399 424 15 345 33 47 162 742
Cuts at: 3558 3643 4714 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 424 399 345 162 85
47 33 15
BspEI T'CCGG_A
Cuts at: 2559 2693 5160 2559
Size: 134 2467 2896
Fragments arranged by size:
2896 2467 134
BspHI T'CATG_A
Cuts at: 3386 5116 3386
Size: 1730 3767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2319 2335 2544 2785 2852 844
Size: 1475 16 209 241 67 3489
Fragments arranged by size:
3489 1475 241 209 67 16
BsrBI CCG'CTC
Cuts at: 1394 1823 3384 1394
Size: 429 1561 3507
Fragments arranged by size:
3507 1561 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3992 4166 65
Size: 1183 2744 174 1396
Fragments arranged by size:
2744 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4141 4328 1700
Size: 110 2331 187 2869
Fragments arranged by size:
2869 2331 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5497
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5497
BssSI C'ACGA_G
Cuts at: 3557 4569 3557
Size: 1012 4485
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5497
BstBI TT'CG_AA
Cuts at: 4301 4301
Size: 5497
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5497
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5497
BstYI r'GATC_y
Cuts at: 1972 2385 2562 2827 3194 3579 3596 5048 5134
Size: 413 177 265 367 385 17 1452 86
Cuts at: 5134 5146 5492 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 413 385 367 346 265 177
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2459 2704 2459
Size: 245 5252
BtgI C'CryG_G
Cuts at: 513 1065 2004 2499 513
Size: 552 939 495 3511
Fragments arranged by size:
3511 939 552 495
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4758
Fragments arranged by size:
4758 683 56
BtsI GCAGTG_nn'
Cuts at: 3013 3807 3827 3013
Size: 794 20 4683
Fragments arranged by size:
4683 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5497
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5462
DraI TTT'AAA
Cuts at: 2801 2801
Size: 5497
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4504 809
Size: 507 3188 1802
Fragments arranged by size:
3188 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2328 2944 3833
Size: 54 1425 50 275 18 498 616 889
Cuts at: 3833 5462 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 616 498 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2944 1537
Size: 275 1132 4090
Fragments arranged by size:
4090 1132 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3427 1353
Size: 2074 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4084 4458 4604 387
Size: 1064 138 2495 374 146 1280
Fragments arranged by size:
2495 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2227 2227
Size: 5497
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2096 2378 2540 3560 4157 4944
Size: 393 401 63 282 162 1020 597 787
Cuts at: 4944 5212 1239
Size: 268 1524
Fragments arranged by size:
1524 1020 787 597 401 393 282 268
162 63
EcoNI CCTnn'n_nnAGG
Cuts at: 2375 2375
Size: 5497
EcoO109I rG'GnC_Cy
Cuts at: 2233 2484 2741 2233
Size: 251 257 4989
Fragments arranged by size:
4989 257 251
EcoRI G'AATT_C
Cuts at: 2806 3184 2806
Size: 378 5119
FspI TGC'GCA
Cuts at: 4003 4003
Size: 5497
HaeII r_GCGC'y
Cuts at: 1678 4644 1678
Size: 2966 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2579 2592 2611 2624 4138
Size: 32 135 32 1007 13 19 13 1514
Cuts at: 4138 4170 4212 4244 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1514 1007 135 42 32 32 32
32 19 13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2158 2841 3087 669
Size: 988 281 220 683 246 3079
Fragments arranged by size:
3079 988 683 281 246 220
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5497
HpaI GTT'AAC
Cuts at: 3087 3087
Size: 5497
KpnI G_GTAC'C
Cuts at: 2822 2822
Size: 5497
MfeI C'AATT_G
Cuts at: 3096 3096
Size: 5497
MluI A'CGCG_T
Cuts at: 5373 5373
Size: 5497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2413 4611 4795 1305
Size: 1108 2198 184 2007
Fragments arranged by size:
2198 2007 1108 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2330 10
Size: 54 1425 343 498 3177
Fragments arranged by size:
3177 1425 498 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2498 3455 3814 3973 518
Size: 648 1332 957 359 159 2042
Fragments arranged by size:
2042 1332 957 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2528 2913 3590 4738 4983 1606
Size: 922 385 677 1148 245 2120
Fragments arranged by size:
2120 1148 922 677 385 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4006
Fragments arranged by size:
4006 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5497
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5497
NotI GC'GGCC_GC
Cuts at: 2944 2944
Size: 5497
NspI r_CATG'y
Cuts at: 1132 1229 2679 2855 4400 1132
Size: 97 1450 176 1545 2229
Fragments arranged by size:
2229 1545 1450 176 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5497
PciI A'CATG_T
Cuts at: 4396 4396
Size: 5497
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5497
PmeI GTTT'AAAC
Cuts at: 2801 2801
Size: 5497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3566 3598 5079 5111 3566
Size: 32 1481 32 3952
Fragments arranged by size:
3952 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2233 2233
Size: 5497
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5497
PsiI TTA'TAA
Cuts at: 3067 3067
Size: 5497
PspXI vC'TCGA_Gb
Cuts at: 2228 2403 2228
Size: 175 5322
PstI C_TGCA'G
Cuts at: 830 2142 2346 2400 2555 2849 830
Size: 1312 204 54 155 294 3478
Fragments arranged by size:
3478 1312 294 204 155 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5497
SacI G_AGCT'C
Cuts at: 2229 2229
Size: 5497
SalI G'TCGA_C
Cuts at: 1936 2839 1936
Size: 903 4594
SbfI CC_TGCA'GG
Cuts at: 2849 2849
Size: 5497
ScaI AGT'ACT
Cuts at: 3745 5457 3745
Size: 1712 3785
SfcI C'TryA_G
Cuts at: 826 1045 2138 2342 2396 2551 2845 3980 4661
Size: 219 1093 204 54 155 294 1135 681
Cuts at: 4661 4852 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1093 681 294 219 204 191
155 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5497
SmaI CCC'GGG
Cuts at: 1872 2632 2824 1872
Size: 760 192 4545
Fragments arranged by size:
4545 760 192
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2228 2403 3601 4502 4764 820
Size: 197 926 285 175 1198 901 262 1553
Fragments arranged by size:
1553 1198 926 901 285 262 197 175
SnaBI TAC'GTA
Cuts at: 493 3288 493
Size: 2795 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5497
SphI G_CATG'C
Cuts at: 1132 2679 2855 1132
Size: 1547 176 3774
Fragments arranged by size:
3774 1547 176
SspI AAT'ATT
Cuts at: 5 52 3421 5
Size: 47 3369 2081
Fragments arranged by size:
3369 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5497
StyI C'CwwG_G
Cuts at: 513 1065 2004 2293 2756 2936 513
Size: 552 939 289 463 180 3074
Fragments arranged by size:
3074 939 552 463 289 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2653 3524 3541 3694 3879 4324 325
Size: 1255 1073 871 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1073 871 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3743 5455 96
Size: 274 80 33 51 1443 1766 1712 138
Fragments arranged by size:
1766 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5457 5489 5457
Size: 32 5465
Tth111I GACn'n_nGTC
Cuts at: 1316 2183 1316
Size: 867 4630
XbaI T'CTAG_A
Cuts at: 2833 2833
Size: 5497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2273 2273
Size: 5497
XhoI C'TCGA_G
Cuts at: 1943 2228 2403 1943
Size: 285 175 5037
Fragments arranged by size:
5037 285 175
XmaI C'CCGG_G
Cuts at: 1870 2630 2822 1870
Size: 760 192 4545
Fragments arranged by size:
4545 760 192
XmnI GAAnn'nnTTC
Cuts at: 3626 3626
Size: 5497
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3305 276
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI