Restriction map for
pFN21AE5320
(Circular) (Six-base) MAPSORT of: pFN21AE5320.seq Check: 7878 from: 1 to: 5578
pFN21AE5320 5578 bp
With 182 enzymes: SgfI *
December 8, 2014 13:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2453 2453
Size: 5578
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3388 278
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2203 2921 1937
Size: 266 718 4594
Fragments arranged by size:
4594 718 266
Acc65I G'GTAC_C
Cuts at: 2468 2899 2468
Size: 431 5147
AclI AA'CG_TT
Cuts at: 1563 3705 4078 1563
Size: 2142 373 3063
Fragments arranged by size:
3063 2142 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2555 3641 5025 5293 1632
Size: 923 1086 1384 268 1917
Fragments arranged by size:
1917 1384 1086 923 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5381
AflIII A'CryG_T
Cuts at: 1203 4477 5454 1203
Size: 3274 977 1327
Fragments arranged by size:
3274 1327 977
AgeI A'CCGG_T
Cuts at: 4409 4409
Size: 5578
AhdI GACnn_n'nnGTC
Cuts at: 4307 4307
Size: 5578
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5578
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5578
AlwNI CAG_nnn'CTG
Cuts at: 2605 3321 4893 2605
Size: 716 1572 3290
Fragments arranged by size:
3290 1572 716
ApaI G_GGCC'C
Cuts at: 2455 2455
Size: 5578
ApaLI G'TGCA_C
Cuts at: 3635 4791 3635
Size: 1156 4422
ApoI r'AATT_y
Cuts at: 1493 2190 2482 2887 3113 3265 5374 1493
Size: 697 292 405 226 152 2109 1697
Fragments arranged by size:
2109 1697 697 405 292 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4132 160
Size: 872 3100 1606
Fragments arranged by size:
3100 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
AvaI C'yCGr_G
Cuts at: 1870 1943 2454 2903 1870
Size: 73 511 449 4545
Fragments arranged by size:
4545 511 449 73
BamHI G'GATC_C
Cuts at: 2908 3275 2908
Size: 367 5211
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2468 2899 4354 618
Size: 325 217 627 681 431 1455 1842
Fragments arranged by size:
1842 1455 681 627 431 325 217
BanII G_rGCy'C
Cuts at: 1391 2455 2897 1391
Size: 1064 442 4072
Fragments arranged by size:
4072 1064 442
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4607
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3769 3803 1903
Size: 34 1832 34 3678
Fragments arranged by size:
3678 1832 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2710 3470 4680 2710
Size: 760 1210 3608
Fragments arranged by size:
3608 1210 760
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5578
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4189 136
Size: 107 122 71 631 605 2517 1525
Fragments arranged by size:
2517 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5573 5573
Size: 5578
BlpI GC'TnA_GC
Cuts at: 2995 2995
Size: 5578
Bme1580I G_kGCm'C
Cuts at: 1790 2455 3639 4795 1790
Size: 665 1184 1156 2573
Fragments arranged by size:
2573 1184 1156 665
BmgBI CAC'GTC
Cuts at: 1318 2049 1318
Size: 731 4847
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3309 4267 442
Size: 416 53 83 399 24 1892 958 1753
Fragments arranged by size:
1892 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4238 1239
Size: 2999 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3703 4568 4866 3703
Size: 865 298 4415
Fragments arranged by size:
4415 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4241 882
Size: 617 2742 2219
Fragments arranged by size:
2742 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3369 493
Size: 711 2165 2702
Fragments arranged by size:
2702 2165 711
BsaBI GATnn'nnATC
Cuts at: 5572 5572
Size: 5578
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3385 3767 4402 275
Size: 53 83 186 936 1852 382 635 1451
Fragments arranged by size:
1852 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4011 4409 4683 4830 5241 4011
Size: 398 274 147 411 4348
Fragments arranged by size:
4348 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2689 2719 1178
Size: 30 165 30 1286 30 4037
Fragments arranged by size:
4037 1286 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5578
BseYI C'CCAG_C
Cuts at: 2566 2598 4781 5277 2566
Size: 32 2183 496 2867
Fragments arranged by size:
2867 2183 496 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2481 1163
Size: 573 745 4260
Fragments arranged by size:
4260 745 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3028 3789 4817 1540
Size: 275 186 1027 761 1028 2301
Fragments arranged by size:
2301 1028 1027 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3639 3724 4795 3639
Size: 85 1071 4422
Fragments arranged by size:
4422 1071 85
BsmI GAATG_Cn'
Cuts at: 3089 3182 3089
Size: 93 5485
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2455 2897 3639 3724 4795 1391
Size: 399 665 442 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 665 442 399 85
BspEI T'CCGG_A
Cuts at: 5241 5241
Size: 5578
BspHI T'CATG_A
Cuts at: 2433 3467 5197 2433
Size: 1034 1730 2814
Fragments arranged by size:
2814 1730 1034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2453 2933 844
Size: 1609 480 3489
Fragments arranged by size:
3489 1609 480
BsrBI CCG'CTC
Cuts at: 1394 1823 3465 1394
Size: 429 1642 3507
Fragments arranged by size:
3507 1642 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4073 4247 65
Size: 1183 2825 174 1396
Fragments arranged by size:
2825 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2824 4222 4409 1700
Size: 110 1014 1398 187 2869
Fragments arranged by size:
2869 1398 1014 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5578
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5578
BssSI C'ACGA_G
Cuts at: 3638 4650 3638
Size: 1012 4566
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5578
BstBI TT'CG_AA
Cuts at: 4382 4382
Size: 5578
BstEII G'GTnAC_C
Cuts at: 1184 2557 1184
Size: 1373 4205
BstXI CCAn_nnnn'nTGG
Cuts at: 2180 2180
Size: 5578
BstYI r'GATC_y
Cuts at: 1972 2908 3275 3660 3677 5129 5215 5227 5573
Size: 936 367 385 17 1452 86 12 346
Cuts at: 5573 1972
Size: 1977
Fragments arranged by size:
1977 1452 936 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2015 513
Size: 552 939 11 4076
Fragments arranged by size:
4076 939 552 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2056 487
Size: 683 56 830 4009
Fragments arranged by size:
4009 830 683 56
BtsI GCAGTG_nn'
Cuts at: 3094 3888 3908 3094
Size: 794 20 4764
Fragments arranged by size:
4764 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5578
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5543
DraI TTT'AAA
Cuts at: 2882 2882
Size: 5578
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4585 809
Size: 507 3269 1802
Fragments arranged by size:
3269 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2655 3025 3914
Size: 54 1425 50 275 18 825 370 889
Cuts at: 3914 5543 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 825 370 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3025 1537
Size: 275 1213 4090
Fragments arranged by size:
4090 1213 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3508 1353
Size: 2155 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4165 4539 4685 387
Size: 1064 138 2576 374 146 1280
Fragments arranged by size:
2576 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2555 3641 4238 5025 5293 1239
Size: 393 923 1086 597 787 268 1524
Fragments arranged by size:
1524 1086 923 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2887 3265 2887
Size: 378 5200
FspI TGC'GCA
Cuts at: 4084 4084
Size: 5578
HaeII r_GCGC'y
Cuts at: 1678 4725 1678
Size: 3047 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2689 2721 2822 2854 4219
Size: 32 135 32 1117 32 101 32 1365
Cuts at: 4219 4251 4293 4325 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1365 1117 135 101 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2922 3168 669
Size: 988 281 984 246 3079
Fragments arranged by size:
3079 988 984 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5578
HpaI GTT'AAC
Cuts at: 3168 3168
Size: 5578
KpnI G_GTAC'C
Cuts at: 2472 2903 2472
Size: 431 5147
MfeI C'AATT_G
Cuts at: 3177 3177
Size: 5578
MluI A'CGCG_T
Cuts at: 5454 5454
Size: 5578
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2708 4692 4876 1305
Size: 1403 1984 184 2007
Fragments arranged by size:
2007 1984 1403 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2657 10
Size: 54 1425 343 825 2931
Fragments arranged by size:
2931 1425 825 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2051 2131 3536 3895 4054 518
Size: 648 885 80 1405 359 159 2042
Fragments arranged by size:
2042 1405 885 648 359 159 80
MspA1I CmG'CkG
Cuts at: 1606 2123 2994 3671 4819 5064 1606
Size: 517 871 677 1148 245 2120
Fragments arranged by size:
2120 1148 871 677 517 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2015 513
Size: 552 939 11 4076
Fragments arranged by size:
4076 939 552 11
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5578
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5578
NotI GC'GGCC_GC
Cuts at: 3025 3025
Size: 5578
NspI r_CATG'y
Cuts at: 1132 1229 2402 2936 4481 1132
Size: 97 1173 534 1545 2229
Fragments arranged by size:
2229 1545 1173 534 97
PasI CC'CwG_GG
Cuts at: 1348 2311 1348
Size: 963 4615
PciI A'CATG_T
Cuts at: 4477 4477
Size: 5578
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2373 2605 1108
Size: 1265 232 4081
Fragments arranged by size:
4081 1265 232
PmeI GTTT'AAAC
Cuts at: 2882 2882
Size: 5578
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3647 3679 5160 5192 3647
Size: 32 1481 32 4033
Fragments arranged by size:
4033 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5578
PsiI TTA'TAA
Cuts at: 2477 3148 2477
Size: 671 4907
PspOMI G'GGCC_C
Cuts at: 2451 2451
Size: 5578
PstI C_TGCA'G
Cuts at: 830 2930 830
Size: 2100 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5578
PvuII CAG'CTG
Cuts at: 2123 2123
Size: 5578
SalI G'TCGA_C
Cuts at: 1936 2920 1936
Size: 984 4594
SbfI CC_TGCA'GG
Cuts at: 2930 2930
Size: 5578
ScaI AGT'ACT
Cuts at: 3826 5538 3826
Size: 1712 3866
SfcI C'TryA_G
Cuts at: 826 1045 2926 4061 4742 4933 826
Size: 219 1881 1135 681 191 1471
Fragments arranged by size:
1881 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5578
SmaI CCC'GGG
Cuts at: 1872 2905 1872
Size: 1033 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3682 4583 4845 820
Size: 197 926 1739 901 262 1553
Fragments arranged by size:
1739 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3369 493
Size: 2876 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5578
SphI G_CATG'C
Cuts at: 1132 2936 1132
Size: 1804 3774
SspI AAT'ATT
Cuts at: 5 52 3502 5
Size: 47 3450 2081
Fragments arranged by size:
3450 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5578
StyI C'CwwG_G
Cuts at: 513 1065 2004 2015 2553 3017 513
Size: 552 939 11 538 464 3074
Fragments arranged by size:
3074 939 552 538 464 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2612 3605 3622 3775 3960 4405 325
Size: 1255 1032 993 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1032 993 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3824 5536 96
Size: 274 80 33 51 1443 1847 1712 138
Fragments arranged by size:
1847 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5538 5570 5538
Size: 32 5546
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5578
XbaI T'CTAG_A
Cuts at: 2914 2914
Size: 5578
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2180 2650 2180
Size: 470 5108
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5578
XmaI C'CCGG_G
Cuts at: 1870 2903 1870
Size: 1033 4545
XmnI GAAnn'nnTTC
Cuts at: 3707 3707
Size: 5578
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3386 276
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI