Restriction map for
pFN21AE5319
(Circular) (Six-base) MAPSORT of: pFN21AE5319.seq Check: 1106 from: 1 to: 5662
pFN21AE5319 5662 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2821 2821
Size: 5662
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3472 278
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3005 1937
Size: 1068 4594
Acc65I G'GTAC_C
Cuts at: 2983 2983
Size: 5662
AclI AA'CG_TT
Cuts at: 1563 3789 4162 1563
Size: 2226 373 3063
Fragments arranged by size:
3063 2226 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3725 5109 5377 1632
Size: 2093 1384 268 1917
Fragments arranged by size:
2093 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5465
AflIII A'CryG_T
Cuts at: 1203 4561 5538 1203
Size: 3358 977 1327
Fragments arranged by size:
3358 1327 977
AgeI A'CCGG_T
Cuts at: 4493 4493
Size: 5662
AhdI GACnn_n'nnGTC
Cuts at: 4391 4391
Size: 5662
AleI CACnn'nnGTG
Cuts at: 1166 2486 1166
Size: 1320 4342
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5662
AlwNI CAG_nnn'CTG
Cuts at: 2132 2461 3405 4977 2132
Size: 329 944 1572 2817
Fragments arranged by size:
2817 1572 944 329
ApaI G_GGCC'C
Cuts at: 2626 2626
Size: 5662
ApaLI G'TGCA_C
Cuts at: 3719 4875 3719
Size: 1156 4506
ApoI r'AATT_y
Cuts at: 1493 2971 3197 3349 5458 1493
Size: 1478 226 152 2109 1697
Fragments arranged by size:
2109 1697 1478 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4216 160
Size: 872 3184 1606
Fragments arranged by size:
3184 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
AvaI C'yCGr_G
Cuts at: 1870 1943 2618 2987 1870
Size: 73 675 369 4545
Fragments arranged by size:
4545 675 369 73
BamHI G'GATC_C
Cuts at: 2992 3359 2992
Size: 367 5295
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2252 2601 2983 4438 618
Size: 325 217 627 465 349 382 1455 1842
Fragments arranged by size:
1842 1455 627 465 382 349 325 217
BanII G_rGCy'C
Cuts at: 1391 2523 2626 2981 1391
Size: 1132 103 355 4072
Fragments arranged by size:
4072 1132 355 103
BbeI G_GCGC'C
Cuts at: 2256 2605 2256
Size: 349 5313
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2861 928
Size: 971 962 3729
Fragments arranged by size:
3729 971 962
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3853 3887 1903
Size: 34 1916 34 3678
Fragments arranged by size:
3678 1916 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3554 4764 3554
Size: 1210 4452
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5662
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4273 136
Size: 107 122 71 631 605 2601 1525
Fragments arranged by size:
2601 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5657 5657
Size: 5662
BlpI GC'TnA_GC
Cuts at: 2462 3079 2462
Size: 617 5045
Bme1580I G_kGCm'C
Cuts at: 1790 2626 3723 4879 1790
Size: 836 1097 1156 2573
Fragments arranged by size:
2573 1156 1097 836
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5662
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3393 4351 442
Size: 416 53 83 399 24 1976 958 1753
Fragments arranged by size:
1976 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2103 1056
Size: 1047 4615
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2361 2759 4322 1239
Size: 1122 398 1563 2579
Fragments arranged by size:
2579 1563 1122 398
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2669 3787 4652 4950 2669
Size: 1118 865 298 3381
Fragments arranged by size:
3381 1118 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4325 882
Size: 617 2826 2219
Fragments arranged by size:
2826 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3453 493
Size: 711 2249 2702
Fragments arranged by size:
2702 2249 711
BsaBI GATnn'nnATC
Cuts at: 5656 5656
Size: 5662
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2073 2253 2298 2602
Size: 53 83 186 936 540 180 45 304
Cuts at: 2602 3469 3851 4486 275
Size: 867 382 635 1451
Fragments arranged by size:
1451 936 867 635 540 382 304 186
180 83 53 45
BsaWI w'CCGG_w
Cuts at: 4095 4493 4767 4914 5325 4095
Size: 398 274 147 411 4432
Fragments arranged by size:
4432 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2359 2389 2795 2819 2825
Size: 30 165 30 956 30 406 24 6
Cuts at: 2825 2849 1178
Size: 24 3991
Fragments arranged by size:
3991 956 406 165 30 30 30 24
24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5662
BseYI C'CCAG_C
Cuts at: 2947 4865 5361 2947
Size: 1918 496 3248
Fragments arranged by size:
3248 1918 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2204 2464 2642 1163
Size: 573 468 260 178 4183
Fragments arranged by size:
4183 573 468 260 178
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2295 3112 3873 4901 1540
Size: 275 186 294 817 761 1028 2301
Fragments arranged by size:
2301 1028 817 761 294 275 186
BsiHKAI G_wGCw'C
Cuts at: 2202 3723 3808 4879 2202
Size: 1521 85 1071 2985
Fragments arranged by size:
2985 1521 1071 85
BsmI GAATG_Cn'
Cuts at: 2546 3173 3266 2546
Size: 627 93 4942
Fragments arranged by size:
4942 627 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2171 2171
Size: 5662
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2523 2626 2981 3723 3808 4879
Size: 399 412 321 103 355 742 85 1071
Cuts at: 4879 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 412 399 355 321 103
85
BspEI T'CCGG_A
Cuts at: 5325 5325
Size: 5662
BspHI T'CATG_A
Cuts at: 3551 5281 3551
Size: 1730 3932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2821 3017 844
Size: 1977 196 3489
Fragments arranged by size:
3489 1977 196
BsrBI CCG'CTC
Cuts at: 1394 1823 2757 2900 3549 1394
Size: 429 934 143 649 3507
Fragments arranged by size:
3507 934 649 429 143
BsrDI GCAATG_nn'
Cuts at: 65 1248 4157 4331 65
Size: 1183 2909 174 1396
Fragments arranged by size:
2909 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2294 4306 4493 1700
Size: 110 484 2012 187 2869
Fragments arranged by size:
2869 2012 484 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5662
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5662
BssSI C'ACGA_G
Cuts at: 3722 4734 3722
Size: 1012 4650
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5662
BstBI TT'CG_AA
Cuts at: 4466 4466
Size: 5662
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5662
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5662
BstYI r'GATC_y
Cuts at: 1972 2992 3359 3744 3761 5213 5299 5311 5657
Size: 1020 367 385 17 1452 86 12 346
Cuts at: 5657 1972
Size: 1977
Fragments arranged by size:
1977 1452 1020 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2814 513
Size: 552 1749 3361
Fragments arranged by size:
3361 1749 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4923
Fragments arranged by size:
4923 683 56
BtsI GCAGTG_nn'
Cuts at: 3178 3972 3992 3178
Size: 794 20 4848
Fragments arranged by size:
4848 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5662
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5627
DraI TTT'AAA
Cuts at: 2966 2966
Size: 5662
DraIII CAC_nnn'GTG
Cuts at: 2278 2278
Size: 5662
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2902 4669 809
Size: 507 1586 1767 1802
Fragments arranged by size:
1802 1767 1586 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2029 2049 2121
Size: 54 1425 50 275 18 199 20 72
Cuts at: 2121 2292 2339 2914 3109 3998 5627 8
Size: 171 47 575 195 889 1629 43
Fragments arranged by size:
1629 1425 889 575 275 199 195 171
72 54 50 47 43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 2292 3109 1537
Size: 275 480 817 4090
Fragments arranged by size:
4090 817 480 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2207 2487 3592 1353
Size: 854 280 1105 3423
Fragments arranged by size:
3423 1105 854 280
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2603 4249 4623 4769 387
Size: 1064 138 1014 1646 374 146 1280
Fragments arranged by size:
1646 1280 1064 1014 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2361 2759 3725 4322 5109 5377 1239
Size: 393 729 398 966 597 787 268 1524
Fragments arranged by size:
1524 966 787 729 597 398 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2051 2867 2051
Size: 816 4846
EcoO109I rG'GnC_Cy
Cuts at: 2497 2622 2629 2497
Size: 125 7 5530
Fragments arranged by size:
5530 125 7
EcoRI G'AATT_C
Cuts at: 2971 3349 2971
Size: 378 5284
FspI TGC'GCA
Cuts at: 4168 4168
Size: 5662
HaeII r_GCGC'y
Cuts at: 1678 2028 2256 2605 4809 1678
Size: 350 228 349 2204 2531
Fragments arranged by size:
2531 2204 350 349 228
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2359 2391 2795 2819 2827
Size: 32 135 32 787 32 404 24 8
Cuts at: 2827 2851 4303 4335 4377 4409 1373
Size: 24 1452 32 42 32 2626
Fragments arranged by size:
2626 1452 787 404 135 42 32 32
32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2160 3006 3252 669
Size: 988 281 222 846 246 3079
Fragments arranged by size:
3079 988 846 281 246 222
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5662
HpaI GTT'AAC
Cuts at: 3252 3252
Size: 5662
KasI G'GCGC_C
Cuts at: 2252 2601 2252
Size: 349 5313
KpnI G_GTAC'C
Cuts at: 2987 2987
Size: 5662
MfeI C'AATT_G
Cuts at: 3261 3261
Size: 5662
MluI A'CGCG_T
Cuts at: 5538 5538
Size: 5662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4776 4960 1305
Size: 3471 184 2007
Fragments arranged by size:
3471 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2031 2051 2123 2916 10
Size: 54 1425 343 199 20 72 793 2756
Fragments arranged by size:
2756 1425 793 343 199 72 54 20
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2193 2279 2486 3620 3979 4138
Size: 648 843 184 86 207 1134 359 159
Cuts at: 4138 518
Size: 2042
Fragments arranged by size:
2042 1134 843 648 359 207 184 159
86
MspA1I CmG'CkG
Cuts at: 1606 2035 2361 2458 2532 2951 3078 3755 4903
Size: 429 326 97 74 419 127 677 1148
Cuts at: 4903 5148 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 429 419 326 245 127
97 74
NaeI GCC'GGC
Cuts at: 2296 2296
Size: 5662
NarI GG'CG_CC
Cuts at: 2253 2602 2253
Size: 349 5313
NcoI C'CATG_G
Cuts at: 513 1065 2814 513
Size: 552 1749 3361
Fragments arranged by size:
3361 1749 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5662
NgoMIV G'CCGG_C
Cuts at: 2294 2294
Size: 5662
NheI G'CTAG_C
Cuts at: 1052 2099 1052
Size: 1047 4615
NotI GC'GGCC_GC
Cuts at: 3109 3109
Size: 5662
NspI r_CATG'y
Cuts at: 1132 1229 2735 3020 4565 1132
Size: 97 1506 285 1545 2229
Fragments arranged by size:
2229 1545 1506 285 97
PasI CC'CwG_GG
Cuts at: 1348 2349 1348
Size: 1001 4661
PciI A'CATG_T
Cuts at: 4561 4561
Size: 5662
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5662
PfoI T'CCnGG_A
Cuts at: 2238 2862 2238
Size: 624 5038
PmeI GTTT'AAAC
Cuts at: 2966 2966
Size: 5662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3731 3763 5244 5276 3731
Size: 32 1481 32 4117
Fragments arranged by size:
4117 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2497 2497
Size: 5662
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5662
PsiI TTA'TAA
Cuts at: 3232 3232
Size: 5662
PspOMI G'GGCC_C
Cuts at: 2622 2622
Size: 5662
PstI C_TGCA'G
Cuts at: 830 2811 2893 3014 830
Size: 1981 82 121 3478
Fragments arranged by size:
3478 1981 121 82
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5662
PvuII CAG'CTG
Cuts at: 2035 2458 2035
Size: 423 5239
SalI G'TCGA_C
Cuts at: 1936 3004 1936
Size: 1068 4594
SapI GCTCTTCn'nnn_
Cuts at: 2207 2207
Size: 5662
SbfI CC_TGCA'GG
Cuts at: 2893 3014 2893
Size: 121 5541
ScaI AGT'ACT
Cuts at: 3910 5622 3910
Size: 1712 3950
SfcI C'TryA_G
Cuts at: 826 1045 2807 2889 3010 4145 4826 5017 826
Size: 219 1762 82 121 1135 681 191 1471
Fragments arranged by size:
1762 1471 1135 681 219 191 121 82
SfoI GGC'GCC
Cuts at: 2254 2603 2254
Size: 349 5313
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5662
SmaI CCC'GGG
Cuts at: 1872 2989 1872
Size: 1117 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2684 3766 4667 4929 820
Size: 197 926 741 1082 901 262 1553
Fragments arranged by size:
1553 1082 926 901 741 262 197
SnaBI TAC'GTA
Cuts at: 493 3453 493
Size: 2960 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5662
SphI G_CATG'C
Cuts at: 1132 3020 1132
Size: 1888 3774
SspI AAT'ATT
Cuts at: 5 52 3586 5
Size: 47 3534 2081
Fragments arranged by size:
3534 2081 47
StuI AGG'CCT
Cuts at: 1517 2783 1517
Size: 1266 4396
StyI C'CwwG_G
Cuts at: 513 1065 2052 2597 2814 3101 513
Size: 552 987 545 217 287 3074
Fragments arranged by size:
3074 987 552 545 287 217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2688 3689 3706 3859 4044 4489 325
Size: 1255 1108 1001 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1108 1001 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3908 5620 96
Size: 274 80 33 51 1443 1931 1712 138
Fragments arranged by size:
1931 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2211 2243 5622 5654 2211
Size: 32 3379 32 2219
Fragments arranged by size:
3379 2219 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2693 1316
Size: 1377 4285
XbaI T'CTAG_A
Cuts at: 2998 2998
Size: 5662
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5662
XmaI C'CCGG_G
Cuts at: 1870 2987 1870
Size: 1117 4545
XmnI GAAnn'nnTTC
Cuts at: 3791 3791
Size: 5662
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3470 276
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI