Restriction map for
pFN21AE5317
(Circular) (Six-base) MAPSORT of: pFN21AE5317.seq Check: 281 from: 1 to: 5572
pFN21AE5317 5572 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3382 278
Size: 53 83 186 936 1846 2468
Fragments arranged by size:
2468 1846 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2084 2642 2915 1937
Size: 147 558 273 4594
Fragments arranged by size:
4594 558 273 147
Acc65I G'GTAC_C
Cuts at: 2893 2893
Size: 5572
AclI AA'CG_TT
Cuts at: 1563 3699 4072 1563
Size: 2136 373 3063
Fragments arranged by size:
3063 2136 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2455 3635 5019 5287 1632
Size: 823 1180 1384 268 1917
Fragments arranged by size:
1917 1384 1180 823 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5375
AflIII A'CryG_T
Cuts at: 1203 4471 5448 1203
Size: 3268 977 1327
Fragments arranged by size:
3268 1327 977
AgeI A'CCGG_T
Cuts at: 4403 4403
Size: 5572
AhdI GACnn_n'nnGTC
Cuts at: 4301 4301
Size: 5572
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5572
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2202 1184
Size: 1018 4554
AlwNI CAG_nnn'CTG
Cuts at: 3315 4887 3315
Size: 1572 4000
ApaLI G'TGCA_C
Cuts at: 3629 4785 3629
Size: 1156 4416
ApoI r'AATT_y
Cuts at: 1493 2697 2783 2881 3107 3259 5368 1493
Size: 1204 86 98 226 152 2109 1697
Fragments arranged by size:
2109 1697 1204 226 152 98 86
AseI AT'TA_AT
Cuts at: 160 1032 4126 160
Size: 872 3094 1606
Fragments arranged by size:
3094 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5572
AvaI C'yCGr_G
Cuts at: 1870 1943 2897 1870
Size: 73 954 4545
Fragments arranged by size:
4545 954 73
BamHI G'GATC_C
Cuts at: 2902 3269 2902
Size: 367 5205
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2893 4348 618
Size: 325 217 627 1106 1455 1842
Fragments arranged by size:
1842 1455 1106 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2402 2891 1391
Size: 1011 489 4072
Fragments arranged by size:
4072 1011 489
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4601
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2335 2369 3763 3797 1903
Size: 34 398 34 1394 34 3678
Fragments arranged by size:
3678 1394 398 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3464 4674 3464
Size: 1210 4362
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5572
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2122 4183 136
Size: 107 122 71 631 605 450 2061 1525
Fragments arranged by size:
2061 1525 631 605 450 122 107 71
BglII A'GATC_T
Cuts at: 5567 5567
Size: 5572
BlpI GC'TnA_GC
Cuts at: 2989 2989
Size: 5572
Bme1580I G_kGCm'C
Cuts at: 1790 2191 3633 4789 1790
Size: 401 1442 1156 2573
Fragments arranged by size:
2573 1442 1156 401
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5572
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3303 4261 442
Size: 416 53 83 399 24 1886 958 1753
Fragments arranged by size:
1886 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5572
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 2355 4232 1239
Size: 974 142 1877 2579
Fragments arranged by size:
2579 1877 974 142
Bpu10I CC'TnA_GC
Cuts at: 2040 2490 2040
Size: 450 5122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3697 4562 4860 3697
Size: 865 298 4409
Fragments arranged by size:
4409 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4235 882
Size: 617 2736 2219
Fragments arranged by size:
2736 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2705 3363 493
Size: 711 1501 658 2702
Fragments arranged by size:
2702 1501 711 658
BsaBI GATnn'nnATC
Cuts at: 5566 5566
Size: 5572
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3379 3761 4396 275
Size: 53 83 186 936 1846 382 635 1451
Fragments arranged by size:
1846 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4005 4403 4677 4824 5235 4005
Size: 398 274 147 411 4342
Fragments arranged by size:
4342 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2406 2436 1178
Size: 30 165 30 1003 30 4314
Fragments arranged by size:
4314 1003 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 2319 1186
Size: 1024 109 4439
Fragments arranged by size:
4439 1024 109
BseYI C'CCAG_C
Cuts at: 2479 2575 4775 5271 2479
Size: 96 2200 496 2780
Fragments arranged by size:
2780 2200 496 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2232 1163
Size: 573 496 4503
Fragments arranged by size:
4503 573 496
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3022 3783 4811 1540
Size: 275 186 1021 761 1028 2301
Fragments arranged by size:
2301 1028 1021 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3633 3718 4789 3633
Size: 85 1071 4416
Fragments arranged by size:
4416 1071 85
BsmI GAATG_Cn'
Cuts at: 3083 3176 3083
Size: 93 5479
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2191 2402 2891 3633 3718 4789 1391
Size: 399 401 211 489 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 489 401 399 211 85
BspEI T'CCGG_A
Cuts at: 5235 5235
Size: 5572
BspHI T'CATG_A
Cuts at: 3461 5191 3461
Size: 1730 3842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2654 2927 844
Size: 1810 273 3489
Fragments arranged by size:
3489 1810 273
BsrBI CCG'CTC
Cuts at: 1394 1823 2685 3459 1394
Size: 429 862 774 3507
Fragments arranged by size:
3507 862 774 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2236 2353 4067 4241 65
Size: 1183 988 117 1714 174 1396
Fragments arranged by size:
1714 1396 1183 988 174 117
BsrFI r'CCGG_y
Cuts at: 1700 1810 4216 4403 1700
Size: 110 2406 187 2869
Fragments arranged by size:
2869 2406 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5572
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5572
BssSI C'ACGA_G
Cuts at: 3632 4644 3632
Size: 1012 4560
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2226 794
Size: 1432 4140
BstBI TT'CG_AA
Cuts at: 4376 4376
Size: 5572
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5572
BstYI r'GATC_y
Cuts at: 1972 2902 3269 3654 3671 5123 5209 5221 5567
Size: 930 367 385 17 1452 86 12 346
Cuts at: 5567 1972
Size: 1977
Fragments arranged by size:
1977 1452 930 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2643 2643
Size: 5572
BtgI C'CryG_G
Cuts at: 513 1065 2433 513
Size: 552 1368 3652
Fragments arranged by size:
3652 1368 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4833
Fragments arranged by size:
4833 683 56
BtsI GCAGTG_nn'
Cuts at: 3088 3882 3902 3088
Size: 794 20 4758
Fragments arranged by size:
4758 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5572
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5537
DraI TTT'AAA
Cuts at: 2876 2876
Size: 5572
DraIII CAC_nnn'GTG
Cuts at: 2026 2026
Size: 5572
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4579 809
Size: 507 3263 1802
Fragments arranged by size:
3263 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2430 2712
Size: 54 1425 50 275 18 243 357 282
Cuts at: 2712 2734 3019 3908 5537 8
Size: 22 285 889 1629 43
Fragments arranged by size:
1629 1425 889 357 285 282 275 243
54 50 43 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 3019 1537
Size: 275 1207 4090
Fragments arranged by size:
4090 1207 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2151 2769 3502 1353
Size: 798 618 733 3423
Fragments arranged by size:
3423 798 733 618
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2552 4159 4533 4679 387
Size: 1064 138 963 1607 374 146 1280
Fragments arranged by size:
1607 1280 1064 963 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2355 2455 3635 4232 5019 5287
Size: 393 581 142 100 1180 597 787 268
Cuts at: 5287 1239
Size: 1524
Fragments arranged by size:
1524 1180 787 597 581 393 268 142
100
EcoNI CCTnn'n_nnAGG
Cuts at: 2145 2145
Size: 5572
EcoO109I rG'GnC_Cy
Cuts at: 2666 2666
Size: 5572
EcoRI G'AATT_C
Cuts at: 2697 2783 2881 3259 2697
Size: 86 98 378 5010
Fragments arranged by size:
5010 378 98 86
FspI TGC'GCA
Cuts at: 4078 4078
Size: 5572
HaeII r_GCGC'y
Cuts at: 1678 4719 1678
Size: 3041 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2842 2874 4213 4245 4287
Size: 32 135 32 1270 32 1339 32 42
Cuts at: 4287 4319 1373
Size: 32 2626
Fragments arranged by size:
2626 1339 1270 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2582 2916 3162 669
Size: 988 281 644 334 246 3079
Fragments arranged by size:
3079 988 644 334 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5572
HpaI GTT'AAC
Cuts at: 3162 3162
Size: 5572
KpnI G_GTAC'C
Cuts at: 2897 2897
Size: 5572
MfeI C'AATT_G
Cuts at: 3171 3171
Size: 5572
MluI A'CGCG_T
Cuts at: 5448 5448
Size: 5572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2743 4686 4870 1305
Size: 1438 1943 184 2007
Fragments arranged by size:
2007 1943 1438 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2075 2432 2714 2736 10
Size: 54 1425 343 243 357 282 22 2846
Fragments arranged by size:
2846 1425 357 343 282 243 54 22
MslI CAynn'nnrTG
Cuts at: 518 1166 3530 3889 4048 518
Size: 648 2364 359 159 2042
Fragments arranged by size:
2364 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2483 2836 2988 3665 4813 5058 1606
Size: 877 353 152 677 1148 245 2120
Fragments arranged by size:
2120 1148 877 677 353 245 152
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5020
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5572
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5572
NotI GC'GGCC_GC
Cuts at: 3019 3019
Size: 5572
NspI r_CATG'y
Cuts at: 1132 1229 2930 4475 1132
Size: 97 1701 1545 2229
Fragments arranged by size:
2229 1701 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5572
PciI A'CATG_T
Cuts at: 4471 4471
Size: 5572
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5572
PmeI GTTT'AAAC
Cuts at: 2876 2876
Size: 5572
PmlI CAC'GTG
Cuts at: 2705 2705
Size: 5572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3641 3673 5154 5186 3641
Size: 32 1481 32 4027
Fragments arranged by size:
4027 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2666 2666
Size: 5572
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5572
PsiI TTA'TAA
Cuts at: 3142 3142
Size: 5572
PstI C_TGCA'G
Cuts at: 830 2365 2924 830
Size: 1535 559 3478
Fragments arranged by size:
3478 1535 559
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5572
PvuII CAG'CTG
Cuts at: 2483 2483
Size: 5572
SalI G'TCGA_C
Cuts at: 1936 2914 1936
Size: 978 4594
SbfI CC_TGCA'GG
Cuts at: 2924 2924
Size: 5572
ScaI AGT'ACT
Cuts at: 3820 5532 3820
Size: 1712 3860
SexAI A'CCwGG_T
Cuts at: 2078 2078
Size: 5572
SfcI C'TryA_G
Cuts at: 826 1045 2085 2361 2920 4055 4736 4927 826
Size: 219 1040 276 559 1135 681 191 1471
Fragments arranged by size:
1471 1135 1040 681 559 276 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5572
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5572
SmaI CCC'GGG
Cuts at: 1872 2899 1872
Size: 1027 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3676 4577 4839 820
Size: 197 926 1733 901 262 1553
Fragments arranged by size:
1733 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3363 493
Size: 2870 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5572
SphI G_CATG'C
Cuts at: 1132 2930 1132
Size: 1798 3774
SspI AAT'ATT
Cuts at: 5 52 3496 5
Size: 47 3444 2081
Fragments arranged by size:
3444 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5572
StyI C'CwwG_G
Cuts at: 513 1065 3011 513
Size: 552 1946 3074
Fragments arranged by size:
3074 1946 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3599 3616 3769 3954 4399 325
Size: 1255 2019 17 153 185 445 1498
Fragments arranged by size:
2019 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2214 2647 3818
Size: 274 80 33 51 1443 237 433 1171
Cuts at: 3818 5530 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1171 433 274 237 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2406 2438 5532 5564 2406
Size: 32 3094 32 2414
Fragments arranged by size:
3094 2414 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5572
XbaI T'CTAG_A
Cuts at: 2908 2908
Size: 5572
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5572
XmaI C'CCGG_G
Cuts at: 1870 2897 1870
Size: 1027 4545
XmnI GAAnn'nnTTC
Cuts at: 3701 3701
Size: 5572
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3380 276
Size: 53 83 186 936 1846 2468
Fragments arranged by size:
2468 1846 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI Bsu36I EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI