Restriction map for
pFN21AE5315
(Circular) (Six-base) MAPSORT of: pFN21AE5315.seq Check: 6132 from: 1 to: 5614
pFN21AE5315 5614 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2316 2764 2316
Size: 448 5166
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2244 3424 278
Size: 53 83 186 936 708 1180 2468
Fragments arranged by size:
2468 1180 936 708 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2957 1937
Size: 1020 4594
Acc65I G'GTAC_C
Cuts at: 2935 2935
Size: 5614
AclI AA'CG_TT
Cuts at: 1563 3741 4114 1563
Size: 2178 373 3063
Fragments arranged by size:
3063 2178 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2521 3677 5061 5329 1632
Size: 889 1156 1384 268 1917
Fragments arranged by size:
1917 1384 1156 889 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5417
AflIII A'CryG_T
Cuts at: 1203 2810 2812 4513 5490 1203
Size: 1607 2 1701 977 1327
Fragments arranged by size:
1701 1607 1327 977 2
AgeI A'CCGG_T
Cuts at: 4445 4445
Size: 5614
AhdI GACnn_n'nnGTC
Cuts at: 4343 4343
Size: 5614
AleI CACnn'nnGTG
Cuts at: 1166 2424 1166
Size: 1258 4356
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2582 2669 1184
Size: 1398 87 4129
Fragments arranged by size:
4129 1398 87
AlwNI CAG_nnn'CTG
Cuts at: 3357 4929 3357
Size: 1572 4042
ApaLI G'TGCA_C
Cuts at: 3671 4827 3671
Size: 1156 4458
ApoI r'AATT_y
Cuts at: 1493 2158 2923 3149 3301 5410 1493
Size: 665 765 226 152 2109 1697
Fragments arranged by size:
2109 1697 765 665 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4168 160
Size: 872 3136 1606
Fragments arranged by size:
3136 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
AvaI C'yCGr_G
Cuts at: 1870 1943 2276 2636 2939 1870
Size: 73 333 360 303 4545
Fragments arranged by size:
4545 360 333 303 73
BamHI G'GATC_C
Cuts at: 2944 3311 2944
Size: 367 5247
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 2069 2313 2566 2752
Size: 325 217 627 227 55 244 253 186
Cuts at: 2752 2935 4390 618
Size: 183 1455 1842
Fragments arranged by size:
1842 1455 627 325 253 244 227 217
186 183 55
BanII G_rGCy'C
Cuts at: 1391 2576 2933 1391
Size: 1185 357 4072
Fragments arranged by size:
4072 1185 357
BbeI G_GCGC'C
Cuts at: 2018 2018
Size: 5614
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2774 928
Size: 971 875 3768
Fragments arranged by size:
3768 971 875
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2560 2594 3805 3839 1903
Size: 34 623 34 1211 34 3678
Fragments arranged by size:
3678 1211 623 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3506 4716 3506
Size: 1210 4404
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5614
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2256 4225 136
Size: 107 122 71 631 605 584 1969 1525
Fragments arranged by size:
1969 1525 631 605 584 122 107 71
BglII A'GATC_T
Cuts at: 5609 5609
Size: 5614
BlpI GC'TnA_GC
Cuts at: 3031 3031
Size: 5614
Bme1580I G_kGCm'C
Cuts at: 1790 2072 2105 2352 3675 4831 1790
Size: 282 33 247 1323 1156 2573
Fragments arranged by size:
2573 1323 1156 282 247 33
BmgBI CAC'GTC
Cuts at: 1318 2382 1318
Size: 1064 4550
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3345 4303 442
Size: 416 53 83 399 24 1928 958 1753
Fragments arranged by size:
1928 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2173 2690 2798 4274 1239
Size: 917 17 517 108 1476 2579
Fragments arranged by size:
2579 1476 917 517 108 17
Bpu10I CC'TnA_GC
Cuts at: 2219 2254 2219
Size: 35 5579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2825 3739 4604 4902 2825
Size: 914 865 298 3537
Fragments arranged by size:
3537 914 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2602 4277 882
Size: 617 1103 1675 2219
Fragments arranged by size:
2219 1675 1103 617
BsaAI yAC'GTr
Cuts at: 493 1204 2065 2813 3405 493
Size: 711 861 748 592 2702
Fragments arranged by size:
2702 861 748 711 592
BsaBI GATnn'nnATC
Cuts at: 5608 5608
Size: 5614
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 2202 2241 3421
Size: 53 83 186 936 482 187 39 1180
Cuts at: 3421 3803 4438 275
Size: 382 635 1451
Fragments arranged by size:
1451 1180 936 635 482 382 187 186
83 53 39
BsaWI w'CCGG_w
Cuts at: 4047 4445 4719 4866 5277 4047
Size: 398 274 147 411 4384
Fragments arranged by size:
4384 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5389
Fragments arranged by size:
5389 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2510 2654 1186
Size: 967 357 144 4146
Fragments arranged by size:
4146 967 357 144
BseYI C'CCAG_C
Cuts at: 2351 2511 2740 2821 4817 5313 2351
Size: 160 229 81 1996 496 2652
Fragments arranged by size:
2652 1996 496 229 160 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2863 1163
Size: 573 1127 3914
Fragments arranged by size:
3914 1127 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2094 2264 3064 3825 4853 1540
Size: 275 186 93 170 800 761 1028 2301
Fragments arranged by size:
2301 1028 800 761 275 186 170 93
BsiHKAI G_wGCw'C
Cuts at: 3675 3760 4831 3675
Size: 85 1071 4458
Fragments arranged by size:
4458 1071 85
BsmI GAATG_Cn'
Cuts at: 3125 3218 3125
Size: 93 5521
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2105 2352 2576 2933 3675 3760
Size: 399 282 33 247 224 357 742 85
Cuts at: 3760 4831 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 357 282 247 224
85 33
BspEI T'CCGG_A
Cuts at: 5277 5277
Size: 5614
BspHI T'CATG_A
Cuts at: 3503 5233 3503
Size: 1730 3884
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2316 2764 2969 844
Size: 1472 448 205 3489
Fragments arranged by size:
3489 1472 448 205
BsrBI CCG'CTC
Cuts at: 1394 1823 2152 2253 3501 1394
Size: 429 329 101 1248 3507
Fragments arranged by size:
3507 1248 429 329 101
BsrDI GCAATG_nn'
Cuts at: 65 1248 4109 4283 65
Size: 1183 2861 174 1396
Fragments arranged by size:
2861 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2017 2170 2259 2421 2437 4258 4445
Size: 110 207 153 89 162 16 1821 187
Cuts at: 4445 1700
Size: 2869
Fragments arranged by size:
2869 1821 207 187 162 153 110 89
16
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5614
BssHII G'CGCG_C
Cuts at: 1925 2035 2037 2039 2110 1925
Size: 110 2 2 71 5429
Fragments arranged by size:
5429 110 71 2 2
BssSI C'ACGA_G
Cuts at: 3674 4686 3674
Size: 1012 4602
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2457 794
Size: 1663 3951
BstBI TT'CG_AA
Cuts at: 4418 4418
Size: 5614
BstEII G'GTnAC_C
Cuts at: 1184 2629 1184
Size: 1445 4169
BstXI CCAn_nnnn'nTGG
Cuts at: 2555 2626 2555
Size: 71 5543
BstYI r'GATC_y
Cuts at: 1972 2788 2944 3311 3696 3713 5165 5251 5263
Size: 816 156 367 385 17 1452 86 12
Cuts at: 5263 5609 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 816 385 367 346 156 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2023 2023
Size: 5614
BtgI C'CryG_G
Cuts at: 513 1065 2163 2733 2827 2908 513
Size: 552 1098 570 94 81 3219
Fragments arranged by size:
3219 1098 570 552 94 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4875
Fragments arranged by size:
4875 683 56
BtsI GCAGTG_nn'
Cuts at: 3130 3924 3944 3130
Size: 794 20 4800
Fragments arranged by size:
4800 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5614
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5579
DraI TTT'AAA
Cuts at: 2918 2918
Size: 5614
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2207 4621 809
Size: 507 891 2414 1802
Fragments arranged by size:
2414 1802 891 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2261 2286
Size: 54 1425 50 275 18 261 170 25
Cuts at: 2286 2319 2406 2730 3061 3950 5579 8
Size: 33 87 324 331 889 1629 43
Fragments arranged by size:
1629 1425 889 331 324 275 261 170
87 54 50 43 33 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 2091 2261 3061 1537
Size: 275 279 170 800 4090
Fragments arranged by size:
4090 800 279 275 170
EarI CTCTTCn'nnn_
Cuts at: 1353 3544 1353
Size: 2191 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4201 4575 4721 387
Size: 1064 138 2612 374 146 1280
Fragments arranged by size:
2612 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2173 2521 2690 2798 3677 4274
Size: 393 524 17 348 169 108 879 597
Cuts at: 4274 5061 5329 1239
Size: 787 268 1524
Fragments arranged by size:
1524 879 787 597 524 393 348 268
169 108 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2138 2288 2138
Size: 150 5464
EcoO109I rG'GnC_Cy
Cuts at: 2281 2293 2329 2281
Size: 12 36 5566
Fragments arranged by size:
5566 36 12
EcoRI G'AATT_C
Cuts at: 2158 2923 3301 2158
Size: 765 378 4471
Fragments arranged by size:
4471 765 378
FseI GG_CCGG'CC
Cuts at: 2263 2263
Size: 5614
FspI TGC'GCA
Cuts at: 4120 4120
Size: 5614
HaeII r_GCGC'y
Cuts at: 1678 2018 2251 2544 4761 1678
Size: 340 233 293 2217 2531
Fragments arranged by size:
2531 2217 340 293 233
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2669 2701 2773 2805 4255
Size: 32 135 32 1097 32 72 32 1450
Cuts at: 4255 4287 4329 4361 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1450 1097 135 72 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2958 3204 669
Size: 988 281 1020 246 3079
Fragments arranged by size:
3079 1020 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5614
HpaI GTT'AAC
Cuts at: 3204 3204
Size: 5614
KasI G'GCGC_C
Cuts at: 2014 2014
Size: 5614
KpnI G_GTAC'C
Cuts at: 2939 2939
Size: 5614
MfeI C'AATT_G
Cuts at: 3213 3213
Size: 5614
MluI A'CGCG_T
Cuts at: 5490 5490
Size: 5614
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4728 4912 1305
Size: 3423 184 2007
Fragments arranged by size:
3423 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2408 10
Size: 54 1425 343 576 3216
Fragments arranged by size:
3216 1425 576 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2424 3572 3931 4090 518
Size: 648 1258 1148 359 159 2042
Fragments arranged by size:
2042 1258 1148 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2555 3030 3707 4855 5100 1606
Size: 949 475 677 1148 245 2120
Fragments arranged by size:
2120 1148 949 677 475 245
NaeI GCC'GGC
Cuts at: 2019 2261 2019
Size: 242 5372
NarI GG'CG_CC
Cuts at: 2015 2015
Size: 5614
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5062
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5614
NgoMIV G'CCGG_C
Cuts at: 2017 2259 2017
Size: 242 5372
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5614
NotI GC'GGCC_GC
Cuts at: 3061 3061
Size: 5614
NspI r_CATG'y
Cuts at: 1132 1229 2972 4517 1132
Size: 97 1743 1545 2229
Fragments arranged by size:
2229 1743 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2794 1348
Size: 1446 4168
PciI A'CATG_T
Cuts at: 4513 4513
Size: 5614
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2065 1108
Size: 957 4657
PfoI T'CCnGG_A
Cuts at: 2384 2384
Size: 5614
PmeI GTTT'AAAC
Cuts at: 2918 2918
Size: 5614
PmlI CAC'GTG
Cuts at: 2813 2813
Size: 5614
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3683 3715 5196 5228 3683
Size: 32 1481 32 4069
Fragments arranged by size:
4069 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2281 2329 2281
Size: 48 5566
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5614
PsiI TTA'TAA
Cuts at: 3184 3184
Size: 5614
PstI C_TGCA'G
Cuts at: 830 2889 2966 830
Size: 2059 77 3478
Fragments arranged by size:
3478 2059 77
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5614
SalI G'TCGA_C
Cuts at: 1936 2956 1936
Size: 1020 4594
SbfI CC_TGCA'GG
Cuts at: 2889 2966 2889
Size: 77 5537
ScaI AGT'ACT
Cuts at: 3862 5574 3862
Size: 1712 3902
SfcI C'TryA_G
Cuts at: 826 1045 2885 2962 4097 4778 4969 826
Size: 219 1840 77 1135 681 191 1471
Fragments arranged by size:
1840 1471 1135 681 219 191 77
SfoI GGC'GCC
Cuts at: 2016 2016
Size: 5614
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
SgrAI Cr'CCGG_yG
Cuts at: 1700 2421 1700
Size: 721 4893
SmaI CCC'GGG
Cuts at: 1872 2941 1872
Size: 1069 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2840 3718 4619 4881 820
Size: 197 926 897 878 901 262 1553
Fragments arranged by size:
1553 926 901 897 878 262 197
SnaBI TAC'GTA
Cuts at: 493 3405 493
Size: 2912 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5614
SphI G_CATG'C
Cuts at: 1132 2972 1132
Size: 1840 3774
SspI AAT'ATT
Cuts at: 5 52 3538 5
Size: 47 3486 2081
Fragments arranged by size:
3486 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5614
StyI C'CwwG_G
Cuts at: 513 1065 2625 3053 513
Size: 552 1560 428 3074
Fragments arranged by size:
3074 1560 552 428
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3641 3658 3811 3996 4441 325
Size: 1255 2061 17 153 185 445 1498
Fragments arranged by size:
2061 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2052 3860 5572
Size: 274 80 33 51 1443 75 1808 1712
Cuts at: 5572 96
Size: 138
Fragments arranged by size:
1808 1712 1443 274 138 80 75 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2851 2883 5574 5606 2851
Size: 32 2691 32 2859
Fragments arranged by size:
2859 2691 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5614
XbaI T'CTAG_A
Cuts at: 2950 2950
Size: 5614
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5614
XmaI C'CCGG_G
Cuts at: 1870 2939 1870
Size: 1069 4545
XmnI GAAnn'nnTTC
Cuts at: 3743 3743
Size: 5614
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2242 3422 276
Size: 53 83 186 936 708 1180 2468
Fragments arranged by size:
2468 1180 936 708 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI
FspAI NruI NsiI PacI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI