Restriction map for
pFN21AE5313
(Circular) (Six-base) MAPSORT of: pFN21AE5313.seq Check: 6459 from: 1 to: 5587
pFN21AE5313 5587 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3397 278
Size: 53 83 186 936 1861 2468
Fragments arranged by size:
2468 1861 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2136 2930 1937
Size: 199 794 4594
Fragments arranged by size:
4594 794 199
Acc65I G'GTAC_C
Cuts at: 2908 2908
Size: 5587
AclI AA'CG_TT
Cuts at: 1563 3714 4087 1563
Size: 2151 373 3063
Fragments arranged by size:
3063 2151 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3650 5034 5302 1632
Size: 2018 1384 268 1917
Fragments arranged by size:
2018 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5390
AflIII A'CryG_T
Cuts at: 1203 2122 2819 4486 5463 1203
Size: 919 697 1667 977 1327
Fragments arranged by size:
1667 1327 977 919 697
AgeI A'CCGG_T
Cuts at: 4418 4418
Size: 5587
AhdI GACnn_n'nnGTC
Cuts at: 4316 4316
Size: 5587
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5587
AlwNI CAG_nnn'CTG
Cuts at: 2590 3330 4902 2590
Size: 740 1572 3275
Fragments arranged by size:
3275 1572 740
ApaLI G'TGCA_C
Cuts at: 2532 3644 4800 2532
Size: 1112 1156 3319
Fragments arranged by size:
3319 1156 1112
ApoI r'AATT_y
Cuts at: 1493 2378 2896 3122 3274 5383 1493
Size: 885 518 226 152 2109 1697
Fragments arranged by size:
2109 1697 885 518 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4141 160
Size: 872 3109 1606
Fragments arranged by size:
3109 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5587
AvaI C'yCGr_G
Cuts at: 1870 1943 2912 1870
Size: 73 969 4545
Fragments arranged by size:
4545 969 73
BamHI G'GATC_C
Cuts at: 2917 3284 2917
Size: 367 5220
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2908 4363 618
Size: 325 217 627 1121 1455 1842
Fragments arranged by size:
1842 1455 1121 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2906 1391
Size: 1515 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4616
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3778 3812 1903
Size: 34 1841 34 3678
Fragments arranged by size:
3678 1841 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2231 3479 4689 2231
Size: 1248 1210 3129
Fragments arranged by size:
3129 1248 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5587
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4198 136
Size: 107 122 71 631 605 2526 1525
Fragments arranged by size:
2526 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5582 5582
Size: 5587
BlpI GC'TnA_GC
Cuts at: 3004 3004
Size: 5587
Bme1580I G_kGCm'C
Cuts at: 1790 2536 3648 4804 1790
Size: 746 1112 1156 2573
Fragments arranged by size:
2573 1156 1112 746
BmgBI CAC'GTC
Cuts at: 1318 2820 1318
Size: 1502 4085
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2677 3318 4276
Size: 416 53 83 399 24 1260 641 958
Cuts at: 4276 442
Size: 1753
Fragments arranged by size:
1753 1260 958 641 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5587
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 5587
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2191 4247 1239
Size: 952 2056 2579
Fragments arranged by size:
2579 2056 952
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2010 3712 4577 4875 2010
Size: 1702 865 298 2722
Fragments arranged by size:
2722 1702 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4250 882
Size: 617 2751 2219
Fragments arranged by size:
2751 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3378 493
Size: 711 2174 2702
Fragments arranged by size:
2702 2174 711
BsaBI GATnn'nnATC
Cuts at: 5581 5581
Size: 5587
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3394 3776 4411 275
Size: 53 83 186 936 1861 382 635 1451
Fragments arranged by size:
1861 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4020 4418 4692 4839 5250 4020
Size: 398 274 147 411 4357
Fragments arranged by size:
4357 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2189 2219 2833 2863 1178
Size: 30 165 30 786 30 614 30 3902
Fragments arranged by size:
3902 786 614 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2640 1186
Size: 1454 4133
BseYI C'CCAG_C
Cuts at: 2272 2348 4790 5286 2272
Size: 76 2442 496 2573
Fragments arranged by size:
2573 2442 496 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5014
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3037 3798 4826 1540
Size: 275 186 1036 761 1028 2301
Fragments arranged by size:
2301 1036 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2536 3648 3733 4804 2536
Size: 1112 85 1071 3319
Fragments arranged by size:
3319 1112 1071 85
BsmI GAATG_Cn'
Cuts at: 2078 3098 3191 2078
Size: 1020 93 4474
Fragments arranged by size:
4474 1020 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2536 2906 3648 3733 4804 1391
Size: 399 746 370 742 85 1071 2174
Fragments arranged by size:
2174 1071 746 742 399 370 85
BspEI T'CCGG_A
Cuts at: 5250 5250
Size: 5587
BspHI T'CATG_A
Cuts at: 2356 2634 2781 3476 5206 2356
Size: 278 147 695 1730 2737
Fragments arranged by size:
2737 1730 695 278 147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2328 2942 844
Size: 1484 614 3489
Fragments arranged by size:
3489 1484 614
BsrBI CCG'CTC
Cuts at: 1394 1823 3474 1394
Size: 429 1651 3507
Fragments arranged by size:
3507 1651 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4082 4256 65
Size: 1183 2834 174 1396
Fragments arranged by size:
2834 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4231 4418 1700
Size: 110 2421 187 2869
Fragments arranged by size:
2869 2421 187 110
BsrGI T'GTAC_A
Cuts at: 96 2125 2822 96
Size: 2029 697 2861
Fragments arranged by size:
2861 2029 697
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5587
BssSI C'ACGA_G
Cuts at: 3647 4659 3647
Size: 1012 4575
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5587
BstBI TT'CG_AA
Cuts at: 4391 4391
Size: 5587
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5587
BstXI CCAn_nnnn'nTGG
Cuts at: 2047 2753 2047
Size: 706 4881
BstYI r'GATC_y
Cuts at: 1972 2917 3284 3669 3686 5138 5224 5236 5582
Size: 945 367 385 17 1452 86 12 346
Cuts at: 5582 1972
Size: 1977
Fragments arranged by size:
1977 1452 945 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2616 2616
Size: 5587
BtgI C'CryG_G
Cuts at: 513 1065 2004 2546 513
Size: 552 939 542 3554
Fragments arranged by size:
3554 939 552 542
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4848
Fragments arranged by size:
4848 683 56
BtsI GCAGTG_nn'
Cuts at: 3103 3897 3917 3103
Size: 794 20 4773
Fragments arranged by size:
4773 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5587
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5552
DraI TTT'AAA
Cuts at: 2891 2891
Size: 5587
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4594 809
Size: 507 3278 1802
Fragments arranged by size:
3278 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3034 3923 5552
Size: 54 1425 50 275 18 1204 889 1629
Cuts at: 5552 8
Size: 43
Fragments arranged by size:
1629 1425 1204 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3034 1537
Size: 275 1222 4090
Fragments arranged by size:
4090 1222 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3517 1353
Size: 2164 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4174 4548 4694 387
Size: 1064 138 2585 374 146 1280
Fragments arranged by size:
2585 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2191 3650 4247 5034 5302 1239
Size: 393 559 1459 597 787 268 1524
Fragments arranged by size:
1524 1459 787 597 559 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2025 2652 2025
Size: 627 4960
EcoRI G'AATT_C
Cuts at: 2378 2896 3274 2378
Size: 518 378 4691
Fragments arranged by size:
4691 518 378
FspI TGC'GCA
Cuts at: 4093 4093
Size: 5587
HaeII r_GCGC'y
Cuts at: 1678 4734 1678
Size: 3056 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2008 2040 2189 2221 2833
Size: 32 135 32 436 32 149 32 612
Cuts at: 2833 2865 4228 4260 4302 4334 1373
Size: 32 1363 32 42 32 2626
Fragments arranged by size:
2626 1363 612 436 149 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2137 2931 3177 669
Size: 988 281 199 794 246 3079
Fragments arranged by size:
3079 988 794 281 246 199
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5587
HpaI GTT'AAC
Cuts at: 3177 3177
Size: 5587
KpnI G_GTAC'C
Cuts at: 2912 2912
Size: 5587
MfeI C'AATT_G
Cuts at: 3186 3186
Size: 5587
MluI A'CGCG_T
Cuts at: 5463 5463
Size: 5587
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4701 4885 1305
Size: 3396 184 2007
Fragments arranged by size:
3396 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3765
Fragments arranged by size:
3765 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2045 2633 3545 3904 4063 518
Size: 648 879 588 912 359 159 2042
Fragments arranged by size:
2042 912 879 648 588 359 159
MspA1I CmG'CkG
Cuts at: 1606 2168 3003 3680 4828 5073 1606
Size: 562 835 677 1148 245 2120
Fragments arranged by size:
2120 1148 835 677 562 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4096
Fragments arranged by size:
4096 939 552
NdeI CA'TA_TG
Cuts at: 387 2130 2149 2326 387
Size: 1743 19 177 3648
Fragments arranged by size:
3648 1743 177 19
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5587
NotI GC'GGCC_GC
Cuts at: 3034 3034
Size: 5587
NspI r_CATG'y
Cuts at: 1132 1229 2126 2945 4490 1132
Size: 97 897 819 1545 2229
Fragments arranged by size:
2229 1545 897 819 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5587
PciI A'CATG_T
Cuts at: 2122 4486 2122
Size: 2364 3223
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5587
PmeI GTTT'AAAC
Cuts at: 2891 2891
Size: 5587
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2655 2687 3656 3688 5169 5201 2655
Size: 32 969 32 1481 32 3041
Fragments arranged by size:
3041 1481 969 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5587
PsiI TTA'TAA
Cuts at: 3157 3157
Size: 5587
PstI C_TGCA'G
Cuts at: 830 2939 830
Size: 2109 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5587
SalI G'TCGA_C
Cuts at: 1936 2135 2929 1936
Size: 199 794 4594
Fragments arranged by size:
4594 794 199
SbfI CC_TGCA'GG
Cuts at: 2939 2939
Size: 5587
ScaI AGT'ACT
Cuts at: 3835 5547 3835
Size: 1712 3875
SfcI C'TryA_G
Cuts at: 826 1045 2935 4070 4751 4942 826
Size: 219 1890 1135 681 191 1471
Fragments arranged by size:
1890 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5587
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5587
SmaI CCC'GGG
Cuts at: 1872 2914 1872
Size: 1042 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2025 3691 4592 4854 820
Size: 197 926 82 1666 901 262 1553
Fragments arranged by size:
1666 1553 926 901 262 197 82
SnaBI TAC'GTA
Cuts at: 493 3378 493
Size: 2885 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5587
SphI G_CATG'C
Cuts at: 1132 2945 1132
Size: 1813 3774
SspI AAT'ATT
Cuts at: 5 52 3511 5
Size: 47 3459 2081
Fragments arranged by size:
3459 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5587
StyI C'CwwG_G
Cuts at: 513 1065 2004 2537 3026 513
Size: 552 939 533 489 3074
Fragments arranged by size:
3074 939 552 533 489
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2087 2801 3614 3631 3784 3969 4414
Size: 1255 507 714 813 17 153 185 445
Cuts at: 4414 325
Size: 1498
Fragments arranged by size:
1498 1255 813 714 507 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2055 2125 2822
Size: 274 80 33 51 1443 78 70 697
Cuts at: 2822 3833 5545 96
Size: 1011 1712 138
Fragments arranged by size:
1712 1443 1011 697 274 138 80 78
70 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2515 2547 5547 5579 2515
Size: 32 3000 32 2523
Fragments arranged by size:
3000 2523 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5587
XbaI T'CTAG_A
Cuts at: 2923 2923
Size: 5587
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2231 2753 2771 2231
Size: 522 18 5047
Fragments arranged by size:
5047 522 18
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5587
XmaI C'CCGG_G
Cuts at: 1870 2912 1870
Size: 1042 4545
XmnI GAAnn'nnTTC
Cuts at: 2516 3716 2516
Size: 1200 4387
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3395 276
Size: 53 83 186 936 1861 2468
Fragments arranged by size:
2468 1861 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI