Restriction map for
pFN21AE5309
(Circular) (Six-base) MAPSORT of: pFN21AE5309.seq Check: 938 from: 1 to: 5530
pFN21AE5309 5530 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3340 278
Size: 53 83 186 936 1804 2468
Fragments arranged by size:
2468 1804 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2873 1937
Size: 936 4594
Acc65I G'GTAC_C
Cuts at: 2851 2851
Size: 5530
AclI AA'CG_TT
Cuts at: 1563 3657 4030 1563
Size: 2094 373 3063
Fragments arranged by size:
3063 2094 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2661 3593 4977 5245 1632
Size: 1029 932 1384 268 1917
Fragments arranged by size:
1917 1384 1029 932 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5333
AflIII A'CryG_T
Cuts at: 1203 2465 4429 5406 1203
Size: 1262 1964 977 1327
Fragments arranged by size:
1964 1327 1262 977
AgeI A'CCGG_T
Cuts at: 4361 4361
Size: 5530
AhdI GACnn_n'nnGTC
Cuts at: 4259 4259
Size: 5530
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5530
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5530
AlwNI CAG_nnn'CTG
Cuts at: 2299 3273 4845 2299
Size: 974 1572 2984
Fragments arranged by size:
2984 1572 974
ApaI G_GGCC'C
Cuts at: 2196 2196
Size: 5530
ApaLI G'TGCA_C
Cuts at: 3587 4743 3587
Size: 1156 4374
ApoI r'AATT_y
Cuts at: 1493 2108 2168 2839 3065 3217 5326 1493
Size: 615 60 671 226 152 2109 1697
Fragments arranged by size:
2109 1697 671 615 226 152 60
AseI AT'TA_AT
Cuts at: 160 1032 4084 160
Size: 872 3052 1606
Fragments arranged by size:
3052 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5530
AvaI C'yCGr_G
Cuts at: 1870 1943 2196 2855 1870
Size: 73 253 659 4545
Fragments arranged by size:
4545 659 253 73
BamHI G'GATC_C
Cuts at: 2047 2860 3227 2047
Size: 813 367 4350
Fragments arranged by size:
4350 813 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2851 4306 618
Size: 325 217 627 1064 1455 1842
Fragments arranged by size:
1842 1455 1064 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2196 2849 1391
Size: 805 653 4072
Fragments arranged by size:
4072 805 653
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4559
BbvCI CC'TCA_GC
Cuts at: 2736 2736
Size: 5530
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3721 3755 1903
Size: 34 1784 34 3678
Fragments arranged by size:
3678 1784 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3422 4632 3422
Size: 1210 4320
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5530
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4141 136
Size: 107 122 71 631 605 2469 1525
Fragments arranged by size:
2469 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2268 2325 5525 2268
Size: 57 3200 2273
Fragments arranged by size:
3200 2273 57
BlpI GC'TnA_GC
Cuts at: 2947 2947
Size: 5530
Bme1580I G_kGCm'C
Cuts at: 1790 2196 3591 4747 1790
Size: 406 1395 1156 2573
Fragments arranged by size:
2573 1395 1156 406
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5530
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3261 4219 442
Size: 416 53 83 399 24 1844 958 1753
Fragments arranged by size:
1844 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5530
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2614 2614
Size: 5530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2040 2201 2309 2489 2688 4190 1239
Size: 801 161 108 180 199 1502 2579
Fragments arranged by size:
2579 1502 801 199 180 161 108
Bpu10I CC'TnA_GC
Cuts at: 2300 2736 2300
Size: 436 5094
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3655 4520 4818 3655
Size: 865 298 4367
Fragments arranged by size:
4367 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4193 882
Size: 617 2694 2219
Fragments arranged by size:
2694 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3321 493
Size: 711 2117 2702
Fragments arranged by size:
2702 2117 711
BsaBI GATnn'nnATC
Cuts at: 5524 5524
Size: 5530
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3337 3719 4354 275
Size: 53 83 186 936 1804 382 635 1451
Fragments arranged by size:
1804 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2053 3963 4361 4635 4782 5193 2053
Size: 1910 398 274 147 411 2390
Fragments arranged by size:
2390 1910 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2604 2634 1178
Size: 30 165 30 1201 30 4074
Fragments arranged by size:
4074 1201 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2330 1186
Size: 1144 4386
BseYI C'CCAG_C
Cuts at: 2422 4733 5229 2422
Size: 2311 496 2723
Fragments arranged by size:
2723 2311 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4957
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2980 3741 4769 1540
Size: 275 186 979 761 1028 2301
Fragments arranged by size:
2301 1028 979 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3591 3676 4747 3591
Size: 85 1071 4374
Fragments arranged by size:
4374 1071 85
BsmI GAATG_Cn'
Cuts at: 2304 3041 3134 2304
Size: 737 93 4700
Fragments arranged by size:
4700 737 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2849 3591 3676 4747 1391
Size: 399 406 653 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 653 406 399 85
BspEI T'CCGG_A
Cuts at: 2053 5193 2053
Size: 3140 2390
BspHI T'CATG_A
Cuts at: 3419 5149 3419
Size: 1730 3800
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2407 2885 844
Size: 1563 478 3489
Fragments arranged by size:
3489 1563 478
BsrBI CCG'CTC
Cuts at: 1394 1823 3417 1394
Size: 429 1594 3507
Fragments arranged by size:
3507 1594 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4025 4199 65
Size: 1183 2777 174 1396
Fragments arranged by size:
2777 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4174 4361 1700
Size: 110 2364 187 2869
Fragments arranged by size:
2869 2364 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5530
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5530
BssSI C'ACGA_G
Cuts at: 3590 4602 3590
Size: 1012 4518
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5530
BstBI TT'CG_AA
Cuts at: 4334 4334
Size: 5530
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5530
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5530
BstYI r'GATC_y
Cuts at: 1972 2047 2268 2325 2860 3227 3612 3629 5081
Size: 75 221 57 535 367 385 17 1452
Cuts at: 5081 5167 5179 5525 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 535 385 367 346 221 86
75 57 17 12
Bsu36I CC'TnA_GG
Cuts at: 2043 2313 2625 2043
Size: 270 312 4948
Fragments arranged by size:
4948 312 270
BtgI C'CryG_G
Cuts at: 513 1065 2004 2450 513
Size: 552 939 446 3593
Fragments arranged by size:
3593 939 552 446
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4791
Fragments arranged by size:
4791 683 56
BtsI GCAGTG_nn'
Cuts at: 2451 3046 3840 3860 2451
Size: 595 794 20 4121
Fragments arranged by size:
4121 794 595 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5530
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5495
DraI TTT'AAA
Cuts at: 2834 2834
Size: 5530
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4537 809
Size: 507 3221 1802
Fragments arranged by size:
3221 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2977 3866 5495
Size: 54 1425 50 275 18 1147 889 1629
Cuts at: 5495 8
Size: 43
Fragments arranged by size:
1629 1425 1147 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2977 1537
Size: 275 1165 4090
Fragments arranged by size:
4090 1165 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3460 1353
Size: 2107 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4117 4491 4637 387
Size: 1064 138 2528 374 146 1280
Fragments arranged by size:
2528 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2040 2201 2309 2489 2661 2688 3593
Size: 393 408 161 108 180 172 27 905
Cuts at: 3593 4190 4977 5245 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 905 787 597 408 393 268 180
172 161 108 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2378 2378
Size: 5530
EcoO109I rG'GnC_Cy
Cuts at: 2193 2419 2193
Size: 226 5304
EcoRI G'AATT_C
Cuts at: 2839 3217 2839
Size: 378 5152
EcoRV GAT'ATC
Cuts at: 2725 2725
Size: 5530
FspI TGC'GCA
Cuts at: 4036 4036
Size: 5530
HaeII r_GCGC'y
Cuts at: 1678 4677 1678
Size: 2999 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4171 4203 4245 4277 1373
Size: 32 135 32 2599 32 42 32 2626
Fragments arranged by size:
2626 2599 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2490 2874 3120 669
Size: 988 281 552 384 246 3079
Fragments arranged by size:
3079 988 552 384 281 246
HindIII A'AGCT_T
Cuts at: 748 2670 748
Size: 1922 3608
HpaI GTT'AAC
Cuts at: 3120 3120
Size: 5530
KpnI G_GTAC'C
Cuts at: 2855 2855
Size: 5530
MfeI C'AATT_G
Cuts at: 3129 3129
Size: 5530
MluI A'CGCG_T
Cuts at: 5406 5406
Size: 5530
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2358 4644 4828 1305
Size: 1053 2286 184 2007
Fragments arranged by size:
2286 2007 1053 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3708
Fragments arranged by size:
3708 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3488 3847 4006 518
Size: 648 843 1479 359 159 2042
Fragments arranged by size:
2042 1479 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2524 2946 3623 4771 5016 1606
Size: 918 422 677 1148 245 2120
Fragments arranged by size:
2120 1148 918 677 422 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2450 513
Size: 552 939 446 3593
Fragments arranged by size:
3593 939 552 446
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5530
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5530
NotI GC'GGCC_GC
Cuts at: 2977 2977
Size: 5530
NspI r_CATG'y
Cuts at: 1132 1229 2469 2888 4433 1132
Size: 97 1240 419 1545 2229
Fragments arranged by size:
2229 1545 1240 419 97
PasI CC'CwG_GG
Cuts at: 1348 2408 1348
Size: 1060 4470
PciI A'CATG_T
Cuts at: 2465 4429 2465
Size: 1964 3566
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5530
PfoI T'CCnGG_A
Cuts at: 2217 2531 2704 2217
Size: 314 173 5043
Fragments arranged by size:
5043 314 173
PmeI GTTT'AAAC
Cuts at: 2834 2834
Size: 5530
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3599 3631 5112 5144 3599
Size: 32 1481 32 3985
Fragments arranged by size:
3985 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2419 2419
Size: 5530
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5530
PsiI TTA'TAA
Cuts at: 3100 3100
Size: 5530
PspOMI G'GGCC_C
Cuts at: 2192 2192
Size: 5530
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2268 2300 2268
Size: 32 5498
PstI C_TGCA'G
Cuts at: 830 2325 2882 830
Size: 1495 557 3478
Fragments arranged by size:
3478 1495 557
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5530
PvuII CAG'CTG
Cuts at: 2524 2524
Size: 5530
SalI G'TCGA_C
Cuts at: 1936 2872 1936
Size: 936 4594
SbfI CC_TGCA'GG
Cuts at: 2882 2882
Size: 5530
ScaI AGT'ACT
Cuts at: 2128 3778 5490 2128
Size: 1650 1712 2168
Fragments arranged by size:
2168 1712 1650
SfcI C'TryA_G
Cuts at: 826 1045 2321 2878 4013 4694 4885 826
Size: 219 1276 557 1135 681 191 1471
Fragments arranged by size:
1471 1276 1135 681 557 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5530
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5530
SmaI CCC'GGG
Cuts at: 1872 2198 2857 1872
Size: 326 659 4545
Fragments arranged by size:
4545 659 326
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3634 4535 4797 820
Size: 197 926 1691 901 262 1553
Fragments arranged by size:
1691 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3321 493
Size: 2828 2702
SpeI A'CTAG_T
Cuts at: 152 2071 152
Size: 1919 3611
SphI G_CATG'C
Cuts at: 1132 2888 1132
Size: 1756 3774
SspI AAT'ATT
Cuts at: 5 52 3454 5
Size: 47 3402 2081
Fragments arranged by size:
3402 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5530
StyI C'CwwG_G
Cuts at: 513 1065 2004 2450 2969 513
Size: 552 939 446 519 3074
Fragments arranged by size:
3074 939 552 519 446
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3557 3574 3727 3912 4357 325
Size: 1255 1977 17 153 185 445 1498
Fragments arranged by size:
1977 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2126 3776 5488
Size: 274 80 33 51 1443 149 1650 1712
Cuts at: 5488 96
Size: 138
Fragments arranged by size:
1712 1650 1443 274 149 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2430 2462 2604 2636 5490 5522 2430
Size: 32 142 32 2854 32 2438
Fragments arranged by size:
2854 2438 142 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5530
XbaI T'CTAG_A
Cuts at: 2866 2866
Size: 5530
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2186 2448 2186
Size: 262 5268
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5530
XmaI C'CCGG_G
Cuts at: 1870 2196 2855 1870
Size: 326 659 4545
Fragments arranged by size:
4545 659 326
XmnI GAAnn'nnTTC
Cuts at: 2148 3659 2148
Size: 1511 4019
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3338 276
Size: 53 83 186 936 1804 2468
Fragments arranged by size:
2468 1804 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI