Restriction map for
pFN21AE5307
(Circular) (Six-base) MAPSORT of: pFN21AE5307.seq Check: 1586 from: 1 to: 5515
pFN21AE5307 5515 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3325 278
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2369 2542 2643 2858 1937
Size: 432 173 101 215 4594
Fragments arranged by size:
4594 432 215 173 101
Acc65I G'GTAC_C
Cuts at: 2836 2836
Size: 5515
AclI AA'CG_TT
Cuts at: 1563 3642 4015 1563
Size: 2079 373 3063
Fragments arranged by size:
3063 2079 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2214 3578 4962 5230 1632
Size: 582 1364 1384 268 1917
Fragments arranged by size:
1917 1384 1364 582 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5318
AflIII A'CryG_T
Cuts at: 1203 4414 5391 1203
Size: 3211 977 1327
Fragments arranged by size:
3211 1327 977
AgeI A'CCGG_T
Cuts at: 4346 4346
Size: 5515
AhdI GACnn_n'nnGTC
Cuts at: 4244 4244
Size: 5515
AleI CACnn'nnGTG
Cuts at: 1166 2726 1166
Size: 1560 3955
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5515
AlwNI CAG_nnn'CTG
Cuts at: 2121 3258 4830 2121
Size: 1137 1572 2806
Fragments arranged by size:
2806 1572 1137
ApaLI G'TGCA_C
Cuts at: 3572 4728 3572
Size: 1156 4359
ApoI r'AATT_y
Cuts at: 1493 2525 2824 3050 3202 5311 1493
Size: 1032 299 226 152 2109 1697
Fragments arranged by size:
2109 1697 1032 299 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4069 160
Size: 872 3037 1606
Fragments arranged by size:
3037 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
AvaI C'yCGr_G
Cuts at: 1870 1943 2174 2840 1870
Size: 73 231 666 4545
Fragments arranged by size:
4545 666 231 73
BamHI G'GATC_C
Cuts at: 2596 2845 3212 2596
Size: 249 367 4899
Fragments arranged by size:
4899 367 249
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2159 2836 4291 618
Size: 325 217 627 372 677 1455 1842
Fragments arranged by size:
1842 1455 677 627 372 325 217
BanII G_rGCy'C
Cuts at: 1391 2676 2834 1391
Size: 1285 158 4072
Fragments arranged by size:
4072 1285 158
BbeI G_GCGC'C
Cuts at: 2163 2163
Size: 5515
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2139 928
Size: 971 240 4304
Fragments arranged by size:
4304 971 240
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3706 3740 1903
Size: 34 1769 34 3678
Fragments arranged by size:
3678 1769 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3407 4617 3407
Size: 1210 4305
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5515
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2144 4126 136
Size: 107 122 71 631 605 472 1982 1525
Fragments arranged by size:
1982 1525 631 605 472 122 107 71
BglII A'GATC_T
Cuts at: 5510 5510
Size: 5515
BlpI GC'TnA_GC
Cuts at: 2932 2932
Size: 5515
Bme1580I G_kGCm'C
Cuts at: 1790 3576 4732 1790
Size: 1786 1156 2573
Fragments arranged by size:
2573 1786 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5515
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2236 3246 4204
Size: 416 53 83 399 24 819 1010 958
Cuts at: 4204 442
Size: 1753
Fragments arranged by size:
1753 1010 958 819 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2143 2586 4175 1239
Size: 904 443 1589 2579
Fragments arranged by size:
2579 1589 904 443
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2812 3640 4505 4803 2812
Size: 828 865 298 3524
Fragments arranged by size:
3524 865 828 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4178 882
Size: 617 2679 2219
Fragments arranged by size:
2679 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3306 493
Size: 711 2102 2702
Fragments arranged by size:
2702 2102 711
BsaBI GATnn'nnATC
Cuts at: 5509 5509
Size: 5515
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2058 2136 2160 3322
Size: 53 83 186 936 525 78 24 1162
Cuts at: 3322 3704 4339 275
Size: 382 635 1451
Fragments arranged by size:
1451 1162 936 635 525 382 186 83
78 53 24
BsaWI w'CCGG_w
Cuts at: 3948 4346 4620 4767 5178 3948
Size: 398 274 147 411 4285
Fragments arranged by size:
4285 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2351 2381 2744 2774 1178
Size: 30 165 30 948 30 363 30 3919
Fragments arranged by size:
3919 948 363 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 2291 2428 1186
Size: 970 135 137 4273
Fragments arranged by size:
4273 970 137 135
BseYI C'CCAG_C
Cuts at: 4718 5214 4718
Size: 496 5019
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2256 1163
Size: 573 520 4422
Fragments arranged by size:
4422 573 520
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2461 2965 3726 4754 1540
Size: 275 186 460 504 761 1028 2301
Fragments arranged by size:
2301 1028 761 504 460 275 186
BsiHKAI G_wGCw'C
Cuts at: 2154 3576 3661 4732 2154
Size: 1422 85 1071 2937
Fragments arranged by size:
2937 1422 1071 85
BsmI GAATG_Cn'
Cuts at: 2502 3026 3119 2502
Size: 524 93 4898
Fragments arranged by size:
4898 524 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 2676 2834 3576 3661 4732 1391
Size: 399 364 522 158 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 522 399 364 158 85
BspEI T'CCGG_A
Cuts at: 5178 5178
Size: 5515
BspHI T'CATG_A
Cuts at: 3404 5134 3404
Size: 1730 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2870 844
Size: 2026 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3402 1394
Size: 429 1579 3507
Fragments arranged by size:
3507 1579 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4010 4184 65
Size: 1183 2762 174 1396
Fragments arranged by size:
2762 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2377 4159 4346 1700
Size: 110 567 1782 187 2869
Fragments arranged by size:
2869 1782 567 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5515
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5515
BssSI C'ACGA_G
Cuts at: 3575 4587 3575
Size: 1012 4503
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5515
BstBI TT'CG_AA
Cuts at: 4319 4319
Size: 5515
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5515
BstYI r'GATC_y
Cuts at: 1972 2596 2845 3212 3597 3614 5066 5152 5164
Size: 624 249 367 385 17 1452 86 12
Cuts at: 5164 5510 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 624 385 367 346 249 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2155 513
Size: 552 939 151 3873
Fragments arranged by size:
3873 939 552 151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2095 487
Size: 683 56 869 3907
Fragments arranged by size:
3907 869 683 56
BtsI GCAGTG_nn'
Cuts at: 3031 3825 3845 3031
Size: 794 20 4701
Fragments arranged by size:
4701 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5480
DraI TTT'AAA
Cuts at: 2819 2819
Size: 5515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4522 809
Size: 507 3206 1802
Fragments arranged by size:
3206 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2962 3851 5480
Size: 54 1425 50 275 18 1132 889 1629
Cuts at: 5480 8
Size: 43
Fragments arranged by size:
1629 1425 1132 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2962 1537
Size: 275 1150 4090
Fragments arranged by size:
4090 1150 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2314 3445 1353
Size: 961 1131 3423
Fragments arranged by size:
3423 1131 961
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4102 4476 4622 387
Size: 1064 138 2513 374 146 1280
Fragments arranged by size:
2513 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2143 2214 2586 3578 4175 4962 5230
Size: 393 511 71 372 992 597 787 268
Cuts at: 5230 1239
Size: 1524
Fragments arranged by size:
1524 992 787 597 511 393 372 268
71
EcoO109I rG'GnC_Cy
Cuts at: 2198 2198
Size: 5515
EcoRI G'AATT_C
Cuts at: 2824 3202 2824
Size: 378 5137
FspI TGC'GCA
Cuts at: 4021 4021
Size: 5515
HaeII r_GCGC'y
Cuts at: 1678 2122 2163 4662 1678
Size: 444 41 2499 2531
Fragments arranged by size:
2531 2499 444 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 2351 2383 2636
Size: 32 135 32 743 32 4 32 253
Cuts at: 2636 2668 2671 2693 2703 2725 4156 4188 4230
Size: 32 3 22 10 22 1431 32 42
Cuts at: 4230 4262 1373
Size: 32 2626
Fragments arranged by size:
2626 1431 743 253 135 42 32 32
32 32 32 32 32 22 22 10
4 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2269 2543 2859 3105 669
Size: 988 281 331 274 316 246 3079
Fragments arranged by size:
3079 988 331 316 281 274 246
HindIII A'AGCT_T
Cuts at: 748 2772 748
Size: 2024 3491
HpaI GTT'AAC
Cuts at: 2269 3105 2269
Size: 836 4679
KasI G'GCGC_C
Cuts at: 2159 2159
Size: 5515
KpnI G_GTAC'C
Cuts at: 2840 2840
Size: 5515
MfeI C'AATT_G
Cuts at: 2448 3114 2448
Size: 666 4849
MluI A'CGCG_T
Cuts at: 5391 5391
Size: 5515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4629 4813 1305
Size: 3324 184 2007
Fragments arranged by size:
3324 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3693
Fragments arranged by size:
3693 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2726 3473 3832 3991 518
Size: 648 1560 747 359 159 2042
Fragments arranged by size:
2042 1560 747 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2046 2157 2606 2931 3608 4756 5001 1606
Size: 440 111 449 325 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 449 440 325 245 111
NaeI GCC'GGC
Cuts at: 2379 2379
Size: 5515
NarI GG'CG_CC
Cuts at: 2160 2160
Size: 5515
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4024
Fragments arranged by size:
4024 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5515
NgoMIV G'CCGG_C
Cuts at: 2377 2377
Size: 5515
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5515
NotI GC'GGCC_GC
Cuts at: 2962 2962
Size: 5515
NspI r_CATG'y
Cuts at: 1132 1229 2873 4418 1132
Size: 97 1644 1545 2229
Fragments arranged by size:
2229 1644 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5515
PciI A'CATG_T
Cuts at: 4414 4414
Size: 5515
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5515
PmeI GTTT'AAAC
Cuts at: 2819 2819
Size: 5515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3584 3616 5097 5129 3584
Size: 32 1481 32 3970
Fragments arranged by size:
3970 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5515
PsiI TTA'TAA
Cuts at: 3085 3085
Size: 5515
PstI C_TGCA'G
Cuts at: 830 2659 2867 830
Size: 1829 208 3478
Fragments arranged by size:
3478 1829 208
PvuI CG_AT'CG
Cuts at: 2001 2461 2001
Size: 460 5055
SacII CC_GC'GG
Cuts at: 2158 2158
Size: 5515
SalI G'TCGA_C
Cuts at: 1936 2541 2857 1936
Size: 605 316 4594
Fragments arranged by size:
4594 605 316
SbfI CC_TGCA'GG
Cuts at: 2867 2867
Size: 5515
ScaI AGT'ACT
Cuts at: 3763 5475 3763
Size: 1712 3803
SfcI C'TryA_G
Cuts at: 826 1045 2640 2655 2863 3998 4679 4870 826
Size: 219 1595 15 208 1135 681 191 1471
Fragments arranged by size:
1595 1471 1135 681 219 208 191 15
SfoI GGC'GCC
Cuts at: 2161 2161
Size: 5515
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5515
SmaI CCC'GGG
Cuts at: 1872 2842 1872
Size: 970 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2791 3619 4520 4782 820
Size: 197 926 848 828 901 262 1553
Fragments arranged by size:
1553 926 901 848 828 262 197
SnaBI TAC'GTA
Cuts at: 493 3306 493
Size: 2813 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5515
SphI G_CATG'C
Cuts at: 1132 2873 1132
Size: 1741 3774
SspI AAT'ATT
Cuts at: 5 52 3439 5
Size: 47 3387 2081
Fragments arranged by size:
3387 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5515
StyI C'CwwG_G
Cuts at: 513 1065 2004 2724 2954 513
Size: 552 939 720 230 3074
Fragments arranged by size:
3074 939 720 552 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2734 3542 3559 3712 3897 4342 325
Size: 1255 1154 808 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1154 808 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2706 3761 5473
Size: 274 80 33 51 1443 729 1055 1712
Cuts at: 5473 96
Size: 138
Fragments arranged by size:
1712 1443 1055 729 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5475 5507 5475
Size: 32 5483
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5515
XbaI T'CTAG_A
Cuts at: 2851 2851
Size: 5515
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5515
XmaI C'CCGG_G
Cuts at: 1870 2840 1870
Size: 970 4545
XmnI GAAnn'nnTTC
Cuts at: 3644 3644
Size: 5515
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3323 276
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI
SwaI XcmI