Restriction map for
pFN21AE5306
(Circular) (Six-base) MAPSORT of: pFN21AE5306.seq Check: 1749 from: 1 to: 5641
pFN21AE5306 5641 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2180 2180
Size: 5641
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2984 1937
Size: 1047 4594
Acc65I G'GTAC_C
Cuts at: 2139 2962 2139
Size: 823 4818
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2522 3704 5088 5356 1632
Size: 890 1182 1384 268 1917
Fragments arranged by size:
1917 1384 1182 890 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 4540 5517 1203
Size: 3337 977 1327
Fragments arranged by size:
3337 1327 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 2544 1166
Size: 1378 4263
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2562 1184
Size: 1378 4263
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2597 2629 2597
Size: 32 5609
AlwNI CAG_nnn'CTG
Cuts at: 2190 2743 2911 3384 4956 2190
Size: 553 168 473 1572 2875
Fragments arranged by size:
2875 1572 553 473 168
ApaI G_GGCC'C
Cuts at: 2386 2804 2841 2897 2386
Size: 418 37 56 5130
Fragments arranged by size:
5130 418 56 37
ApaLI G'TGCA_C
Cuts at: 2537 3698 4854 2537
Size: 1161 1156 3324
Fragments arranged by size:
3324 1161 1156
ApoI r'AATT_y
Cuts at: 1493 2950 3176 3328 5437 1493
Size: 1457 226 152 2109 1697
Fragments arranged by size:
2109 1697 1457 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2150 2593 2789 2966 1870
Size: 73 207 443 196 177 4545
Fragments arranged by size:
4545 443 207 196 177 73
BamHI G'GATC_C
Cuts at: 2971 3338 2971
Size: 367 5274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2139 2962 4417 618
Size: 325 217 627 352 823 1455 1842
Fragments arranged by size:
1842 1455 823 627 352 325 217
BanII G_rGCy'C
Cuts at: 1391 2386 2408 2723 2804 2841 2897 2960 1391
Size: 995 22 315 81 37 56 63 4072
Fragments arranged by size:
4072 995 315 81 63 56 37 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4670
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3832 3866 1903
Size: 34 1895 34 3678
Fragments arranged by size:
3678 1895 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3533 4743 3533
Size: 1210 4431
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2768 2845 4252
Size: 107 122 71 631 605 1096 77 1407
Cuts at: 4252 136
Size: 1525
Fragments arranged by size:
1525 1407 1096 631 605 122 107 77
71
BglII A'GATC_T
Cuts at: 2443 5636 2443
Size: 3193 2448
BlpI GC'TnA_GC
Cuts at: 3058 3058
Size: 5641
Bme1580I G_kGCm'C
Cuts at: 1790 2322 2386 2541 2551 2804 2841 2897 3702
Size: 532 64 155 10 253 37 56 805
Cuts at: 3702 4858 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 805 532 253 155 64 56
37 10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2086 3372 4330
Size: 416 53 83 399 24 669 1286 958
Cuts at: 4330 442
Size: 1753
Fragments arranged by size:
1753 1286 958 669 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2737 4301 1239
Size: 1498 1564 2579
Fragments arranged by size:
2579 1564 1498
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3766 4631 4929 3766
Size: 865 298 4478
Fragments arranged by size:
4478 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4304 882
Size: 617 2805 2219
Fragments arranged by size:
2805 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3432 493
Size: 711 2228 2702
Fragments arranged by size:
2702 2228 711
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3448 3830 4465 275
Size: 53 83 186 936 1915 382 635 1451
Fragments arranged by size:
1915 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2043 2932 4074 4472 4746 4893 5304 2043
Size: 889 1142 398 274 147 411 2380
Fragments arranged by size:
2380 1142 889 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2015 2045 1178
Size: 30 165 30 612 30 4774
Fragments arranged by size:
4774 612 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2841 1186
Size: 1655 3986
BseYI C'CCAG_C
Cuts at: 4844 5340 4844
Size: 496 5145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2612 2662 2777 1163
Size: 573 876 50 115 4027
Fragments arranged by size:
4027 876 573 115 50
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2612 2845 3091 3852 4880 1540
Size: 275 186 611 233 246 761 1028 2301
Fragments arranged by size:
2301 1028 761 611 275 246 233 186
BsiHKAI G_wGCw'C
Cuts at: 2541 3702 3787 4858 2541
Size: 1161 85 1071 3324
Fragments arranged by size:
3324 1161 1071 85
BsmI GAATG_Cn'
Cuts at: 3152 3245 3152
Size: 93 5548
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2322 2386 2408 2541 2551 2723 2804
Size: 399 532 64 22 133 10 172 81
Cuts at: 2804 2841 2897 2960 3702 3787 4858 1391
Size: 37 56 63 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 532 399 172 133 85
81 64 63 56 37 22 10
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 3530 5260 3530
Size: 1730 3911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2180 2649 2996 844
Size: 1336 469 347 3489
Fragments arranged by size:
3489 1336 469 347
BsrBI CCG'CTC
Cuts at: 1394 1823 2281 2509 3528 1394
Size: 429 458 228 1019 3507
Fragments arranged by size:
3507 1019 458 429 228
BsrDI GCAATG_nn'
Cuts at: 65 1248 2306 4136 4310 65
Size: 1183 1058 1830 174 1396
Fragments arranged by size:
1830 1396 1183 1058 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2252 2607 2844 4285 4472 1700
Size: 110 442 355 237 1441 187 2869
Fragments arranged by size:
2869 1441 442 355 237 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 2447 2753 3701 4713 2447
Size: 306 948 1012 3375
Fragments arranged by size:
3375 1012 948 306
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5641
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 2338 1184
Size: 1154 4487
BstXI CCAn_nnnn'nTGG
Cuts at: 2357 2357
Size: 5641
BstYI r'GATC_y
Cuts at: 1972 2443 2971 3338 3723 3740 5192 5278 5290
Size: 471 528 367 385 17 1452 86 12
Cuts at: 5290 5636 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 528 471 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2562 2652 513
Size: 552 939 558 90 3502
Fragments arranged by size:
3502 939 558 552 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 2539 2551 2729 2912 3157 3951 3971 2539
Size: 12 178 183 245 794 20 4209
Fragments arranged by size:
4209 794 245 183 178 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DraIII CAC_nnn'GTG
Cuts at: 2394 2563 2664 2394
Size: 169 101 5371
Fragments arranged by size:
5371 169 101
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2157 2609 2842
Size: 54 1425 50 275 18 327 452 233
Cuts at: 2842 3088 3977 5606 8
Size: 246 889 1629 43
Fragments arranged by size:
1629 1425 889 452 327 275 246 233
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2609 2842 3088 1537
Size: 275 797 233 246 4090
Fragments arranged by size:
4090 797 275 246 233
EarI CTCTTCn'nnn_
Cuts at: 1353 3571 1353
Size: 2218 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2522 2737 3704 4301 5088 5356 1239
Size: 393 890 215 967 597 787 268 1524
Fragments arranged by size:
1524 967 890 787 597 393 268 215
EcoNI CCTnn'n_nnAGG
Cuts at: 2240 2240
Size: 5641
EcoO109I rG'GnC_Cy
Cuts at: 2800 2801 2893 2894 2800
Size: 1 92 1 5547
Fragments arranged by size:
5547 92 1 1
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
FseI GG_CCGG'CC
Cuts at: 2848 2848
Size: 5641
FspI TGC'GCA
Cuts at: 2645 4147 2645
Size: 1502 4139
HaeII r_GCGC'y
Cuts at: 1678 2225 4788 1678
Size: 547 2563 2531
Fragments arranged by size:
2563 2531 547
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 2471 2503 4282
Size: 32 135 32 443 32 424 32 1779
Cuts at: 4282 4314 4356 4388 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1779 443 424 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2068 2985 3231 669
Size: 988 281 130 917 246 3079
Fragments arranged by size:
3079 988 917 281 246 130
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5641
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KpnI G_GTAC'C
Cuts at: 2143 2966 2143
Size: 823 4818
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2895 4755 4939 1305
Size: 1590 1860 184 2007
Fragments arranged by size:
2007 1860 1590 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3819
Fragments arranged by size:
3819 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2544 3599 3958 4117 518
Size: 648 1378 1055 359 159 2042
Fragments arranged by size:
2042 1378 1055 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2134 2200 2217 2530 2890 3057 3734 4882
Size: 528 66 17 313 360 167 677 1148
Cuts at: 4882 5127 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 528 360 313 245 167
66 17
NaeI GCC'GGC
Cuts at: 2254 2846 2254
Size: 592 5049
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4150
Fragments arranged by size:
4150 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NgoMIV G'CCGG_C
Cuts at: 2252 2844 2252
Size: 592 5049
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2999 4544 1132
Size: 97 1770 1545 2229
Fragments arranged by size:
2229 1770 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2551 1348
Size: 1203 4438
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2200 2563 1108
Size: 1092 363 4186
Fragments arranged by size:
4186 1092 363
PfoI T'CCnGG_A
Cuts at: 2325 2325
Size: 5641
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PspOMI G'GGCC_C
Cuts at: 2382 2800 2837 2893 2382
Size: 418 37 56 5130
Fragments arranged by size:
5130 418 56 37
PstI C_TGCA'G
Cuts at: 830 2127 2546 2739 2907 2993 830
Size: 1297 419 193 168 86 3478
Fragments arranged by size:
3478 1297 419 193 168 86
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
PvuII CAG'CTG
Cuts at: 2134 2200 2530 2134
Size: 66 330 5245
Fragments arranged by size:
5245 330 66
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SfcI C'TryA_G
Cuts at: 826 1045 2123 2542 2735 2903 2989 4124 4805
Size: 219 1078 419 193 168 86 1135 681
Cuts at: 4805 4996 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1078 681 419 219 193 191
168 86
SfiI GGCCn_nnn'nGGCC
Cuts at: 2768 2845 2768
Size: 77 5564
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2968 1872
Size: 1096 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3745 4646 4908 820
Size: 197 926 1802 901 262 1553
Fragments arranged by size:
1802 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 2762 1517
Size: 1245 4396
StyI C'CwwG_G
Cuts at: 513 1065 2004 2292 3080 513
Size: 552 939 288 788 3074
Fragments arranged by size:
3074 939 788 552 288
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3668 3685 3838 4023 4468 325
Size: 1255 2088 17 153 185 445 1498
Fragments arranged by size:
2088 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3887 5599 96
Size: 274 80 33 51 1443 1910 1712 138
Fragments arranged by size:
1910 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2648 2680 5601 5633 2648
Size: 32 2921 32 2656
Fragments arranged by size:
2921 2656 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2299 2299
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2966 1870
Size: 1096 4545
XmnI GAAnn'nnTTC
Cuts at: 3770 3770
Size: 5641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI
FspAI KasI NarI NruI NsiI PacI PmlI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfoI SrfI SwaI