Restriction map for
pFN21AE5304
(Circular) (Six-base) MAPSORT of: pFN21AE5304.seq Check: 3301 from: 1 to: 5503
pFN21AE5304 5503 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2388 2581 3313 278
Size: 53 83 186 936 852 193 732 2468
Fragments arranged by size:
2468 936 852 732 193 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2846 1937
Size: 909 4594
Acc65I G'GTAC_C
Cuts at: 2824 2824
Size: 5503
AclI AA'CG_TT
Cuts at: 1563 3630 4003 1563
Size: 2067 373 3063
Fragments arranged by size:
3063 2067 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3566 4950 5218 1632
Size: 1934 1384 268 1917
Fragments arranged by size:
1934 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2291 820
Size: 197 1274 4032
Fragments arranged by size:
4032 1274 197
AflIII A'CryG_T
Cuts at: 1203 4402 5379 1203
Size: 3199 977 1327
Fragments arranged by size:
3199 1327 977
AgeI A'CCGG_T
Cuts at: 4334 4334
Size: 5503
AhdI GACnn_n'nnGTC
Cuts at: 2405 2582 4232 2405
Size: 177 1650 3676
Fragments arranged by size:
3676 1650 177
AleI CACnn'nnGTG
Cuts at: 1166 2441 2621 2715 1166
Size: 1275 180 94 3954
Fragments arranged by size:
3954 1275 180 94
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5503
AlwNI CAG_nnn'CTG
Cuts at: 2263 2344 3246 4818 2263
Size: 81 902 1572 2948
Fragments arranged by size:
2948 1572 902 81
ApaLI G'TGCA_C
Cuts at: 3560 4716 3560
Size: 1156 4347
ApoI r'AATT_y
Cuts at: 1493 2116 2812 3038 3190 5299 1493
Size: 623 696 226 152 2109 1697
Fragments arranged by size:
2109 1697 696 623 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4057 160
Size: 872 3025 1606
Fragments arranged by size:
3025 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
AvaI C'yCGr_G
Cuts at: 1870 1943 2352 2828 1870
Size: 73 409 476 4545
Fragments arranged by size:
4545 476 409 73
BamHI G'GATC_C
Cuts at: 2488 2833 3200 2488
Size: 345 367 4791
Fragments arranged by size:
4791 367 345
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2824 4279 618
Size: 325 217 627 1037 1455 1842
Fragments arranged by size:
1842 1455 1037 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2822 1391
Size: 1431 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2500 2600 2640 928
Size: 971 601 100 40 3791
Fragments arranged by size:
3791 971 601 100 40
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2590 2624 3694 3728 1903
Size: 34 50 34 569 34 1070 34 3678
Fragments arranged by size:
3678 1070 569 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3395 4605 3395
Size: 1210 4293
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5503
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4114 136
Size: 107 122 71 631 605 2442 1525
Fragments arranged by size:
2442 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5498 5498
Size: 5503
BlpI GC'TnA_GC
Cuts at: 2460 2652 2920 2460
Size: 192 268 5043
Fragments arranged by size:
5043 268 192
Bme1580I G_kGCm'C
Cuts at: 1790 3564 4720 1790
Size: 1774 1156 2573
Fragments arranged by size:
2573 1774 1156
BmgBI CAC'GTC
Cuts at: 1318 2485 1318
Size: 1167 4336
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2582 2722 2807
Size: 416 53 83 399 24 1165 140 85
Cuts at: 2807 3234 4192 442
Size: 427 958 1753
Fragments arranged by size:
1753 1165 958 427 416 399 140 85
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5503
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2228 2228
Size: 5503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4163 1239
Size: 2924 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3628 4493 4791 3628
Size: 865 298 4340
Fragments arranged by size:
4340 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2145 2392 4166 882
Size: 617 646 247 1774 2219
Fragments arranged by size:
2219 1774 646 617 247
BsaAI yAC'GTr
Cuts at: 493 1204 3294 493
Size: 711 2090 2702
Fragments arranged by size:
2702 2090 711
BsaBI GATnn'nnATC
Cuts at: 5497 5497
Size: 5503
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2385 2578 3310 3692
Size: 53 83 186 936 852 193 732 382
Cuts at: 3692 4327 275
Size: 635 1451
Fragments arranged by size:
1451 936 852 732 635 382 193 186
83 53
BsaWI w'CCGG_w
Cuts at: 3936 4334 4608 4755 5166 3936
Size: 398 274 147 411 4273
Fragments arranged by size:
4273 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5278
Fragments arranged by size:
5278 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5503
BseYI C'CCAG_C
Cuts at: 2050 4706 5202 2050
Size: 2656 496 2351
Fragments arranged by size:
2656 2351 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2584 1163
Size: 573 848 4082
Fragments arranged by size:
4082 848 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2953 3714 4742 1540
Size: 275 186 952 761 1028 2301
Fragments arranged by size:
2301 1028 952 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3564 3649 4720 3564
Size: 85 1071 4347
Fragments arranged by size:
4347 1071 85
BsmI GAATG_Cn'
Cuts at: 3014 3107 3014
Size: 93 5410
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2822 3564 3649 4720 1391
Size: 399 1032 742 85 1071 2174
Fragments arranged by size:
2174 1071 1032 742 399 85
BspEI T'CCGG_A
Cuts at: 5166 5166
Size: 5503
BspHI T'CATG_A
Cuts at: 3392 5122 3392
Size: 1730 3773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2606 2858 844
Size: 1762 252 3489
Fragments arranged by size:
3489 1762 252
BsrBI CCG'CTC
Cuts at: 1394 1823 3390 1394
Size: 429 1567 3507
Fragments arranged by size:
3507 1567 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2728 3998 4172 65
Size: 1183 1480 1270 174 1396
Fragments arranged by size:
1480 1396 1270 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4147 4334 1700
Size: 110 2337 187 2869
Fragments arranged by size:
2869 2337 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5503
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5503
BssSI C'ACGA_G
Cuts at: 3563 4575 3563
Size: 1012 4491
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5503
BstBI TT'CG_AA
Cuts at: 4307 4307
Size: 5503
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5503
BstXI CCAn_nnnn'nTGG
Cuts at: 2443 2500 2797 2443
Size: 57 297 5149
Fragments arranged by size:
5149 297 57
BstYI r'GATC_y
Cuts at: 1972 2488 2700 2833 3200 3585 3602 5054 5140
Size: 516 212 133 367 385 17 1452 86
Cuts at: 5140 5152 5498 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 516 385 367 346 212 133
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4951
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4764
Fragments arranged by size:
4764 683 56
BtsI GCAGTG_nn'
Cuts at: 3019 3813 3833 3019
Size: 794 20 4689
Fragments arranged by size:
4689 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5503
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5468
DraI TTT'AAA
Cuts at: 2807 2807
Size: 5503
DraIII CAC_nnn'GTG
Cuts at: 2485 2485
Size: 5503
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2577 4510 809
Size: 507 1261 1933 1802
Fragments arranged by size:
1933 1802 1261 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2950 3839 5468
Size: 54 1425 50 275 18 1120 889 1629
Cuts at: 5468 8
Size: 43
Fragments arranged by size:
1629 1425 1120 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2950 1537
Size: 275 1138 4090
Fragments arranged by size:
4090 1138 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2390 3433 1353
Size: 1037 1043 3423
Fragments arranged by size:
3423 1043 1037
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2693 4090 4464 4610 387
Size: 1064 138 1104 1397 374 146 1280
Fragments arranged by size:
1397 1280 1104 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3566 4163 4950 5218 1239
Size: 393 1934 597 787 268 1524
Fragments arranged by size:
1934 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2260 2539 2732 2260
Size: 279 193 5031
Fragments arranged by size:
5031 279 193
EcoRI G'AATT_C
Cuts at: 2812 3190 2812
Size: 378 5125
FspI TGC'GCA
Cuts at: 4009 4009
Size: 5503
HaeII r_GCGC'y
Cuts at: 1678 2646 4650 1678
Size: 968 2004 2531
Fragments arranged by size:
2531 2004 968
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2121 2153 2391 2423 2568
Size: 32 135 32 549 32 238 32 145
Cuts at: 2568 2600 2692 2724 4144 4176 4218 4250 1373
Size: 32 92 32 1420 32 42 32 2626
Fragments arranged by size:
2626 1420 549 238 145 135 92 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2425 2847 3093 669
Size: 988 281 487 422 246 3079
Fragments arranged by size:
3079 988 487 422 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5503
HpaI GTT'AAC
Cuts at: 2425 3093 2425
Size: 668 4835
KpnI G_GTAC'C
Cuts at: 2828 2828
Size: 5503
MfeI C'AATT_G
Cuts at: 3102 3102
Size: 5503
MluI A'CGCG_T
Cuts at: 5379 5379
Size: 5503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4617 4801 1305
Size: 3312 184 2007
Fragments arranged by size:
3312 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3681
Fragments arranged by size:
3681 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2441 2621 2715 3461 3820 3979 518
Size: 648 1275 180 94 746 359 159 2042
Fragments arranged by size:
2042 1275 746 648 359 180 159 94
MspA1I CmG'CkG
Cuts at: 1606 2054 2197 2287 2464 2919 3596 4744 4989
Size: 448 143 90 177 455 677 1148 245
Cuts at: 4989 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 455 448 245 177 143
90
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4951
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5503
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5503
NotI GC'GGCC_GC
Cuts at: 2950 2950
Size: 5503
NspI r_CATG'y
Cuts at: 1132 1229 2861 4406 1132
Size: 97 1632 1545 2229
Fragments arranged by size:
2229 1632 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5503
PciI A'CATG_T
Cuts at: 4402 4402
Size: 5503
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2263 1108
Size: 1155 4348
PmeI GTTT'AAAC
Cuts at: 2807 2807
Size: 5503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3572 3604 5085 5117 3572
Size: 32 1481 32 3958
Fragments arranged by size:
3958 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2539 2732 2539
Size: 193 5310
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5503
PsiI TTA'TAA
Cuts at: 3073 3073
Size: 5503
PstI C_TGCA'G
Cuts at: 830 2855 830
Size: 2025 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5503
PvuII CAG'CTG
Cuts at: 2054 2287 2464 2054
Size: 233 177 5093
Fragments arranged by size:
5093 233 177
SalI G'TCGA_C
Cuts at: 1936 2845 1936
Size: 909 4594
SanDI GG'GwC_CC
Cuts at: 2539 2539
Size: 5503
SbfI CC_TGCA'GG
Cuts at: 2855 2855
Size: 5503
ScaI AGT'ACT
Cuts at: 3751 5463 3751
Size: 1712 3791
SfcI C'TryA_G
Cuts at: 826 1045 2851 3986 4667 4858 826
Size: 219 1806 1135 681 191 1471
Fragments arranged by size:
1806 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5503
SmaI CCC'GGG
Cuts at: 1872 2354 2830 1872
Size: 482 476 4545
Fragments arranged by size:
4545 482 476
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2291 3607 4508 4770 820
Size: 197 926 348 1316 901 262 1553
Fragments arranged by size:
1553 1316 926 901 348 262 197
SnaBI TAC'GTA
Cuts at: 493 3294 493
Size: 2801 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5503
SphI G_CATG'C
Cuts at: 1132 2861 1132
Size: 1729 3774
SrfI GCCC'GGGC
Cuts at: 2354 2354
Size: 5503
SspI AAT'ATT
Cuts at: 5 52 3427 5
Size: 47 3375 2081
Fragments arranged by size:
3375 2081 47
StuI AGG'CCT
Cuts at: 1517 2110 1517
Size: 593 4910
StyI C'CwwG_G
Cuts at: 513 1065 2025 2439 2942 513
Size: 552 960 414 503 3074
Fragments arranged by size:
3074 960 552 503 414
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2270 2789 3530 3547 3700 3885 4330
Size: 1255 690 519 741 17 153 185 445
Cuts at: 4330 325
Size: 1498
Fragments arranged by size:
1498 1255 741 690 519 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2307 3749 5461
Size: 274 80 33 51 1443 330 1442 1712
Cuts at: 5461 96
Size: 138
Fragments arranged by size:
1712 1443 1442 330 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2022 2054 5463 5495 2022
Size: 32 3409 32 2030
Fragments arranged by size:
3409 2030 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5503
XbaI T'CTAG_A
Cuts at: 2839 2839
Size: 5503
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5503
XmaI C'CCGG_G
Cuts at: 1870 2352 2828 1870
Size: 482 476 4545
Fragments arranged by size:
4545 482 476
XmnI GAAnn'nnTTC
Cuts at: 3632 3632
Size: 5503
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2386 2579 3311 276
Size: 53 83 186 936 852 193 732 2468
Fragments arranged by size:
2468 936 852 732 193 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SfoI
SwaI XcmI