Restriction map for
pFN21AE5302
(Circular) (Six-base) MAPSORT of: pFN21AE5302.seq Check: 9181 from: 1 to: 5536
pFN21AE5302 5536 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2647 2647
Size: 5536
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3346 278
Size: 53 83 186 936 1810 2468
Fragments arranged by size:
2468 1810 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2879 1937
Size: 942 4594
Acc65I G'GTAC_C
Cuts at: 2052 2857 2052
Size: 805 4731
AclI AA'CG_TT
Cuts at: 1563 3663 4036 1563
Size: 2100 373 3063
Fragments arranged by size:
3063 2100 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3599 4983 5251 1632
Size: 1967 1384 268 1917
Fragments arranged by size:
1967 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5339
AflIII A'CryG_T
Cuts at: 1203 2352 4435 5412 1203
Size: 1149 2083 977 1327
Fragments arranged by size:
2083 1327 1149 977
AgeI A'CCGG_T
Cuts at: 4367 4367
Size: 5536
AhdI GACnn_n'nnGTC
Cuts at: 4265 4265
Size: 5536
AleI CACnn'nnGTG
Cuts at: 1166 2777 1166
Size: 1611 3925
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5536
AlwNI CAG_nnn'CTG
Cuts at: 2653 2819 3279 4851 2653
Size: 166 460 1572 3338
Fragments arranged by size:
3338 1572 460 166
ApaI G_GGCC'C
Cuts at: 2035 2273 2035
Size: 238 5298
ApaLI G'TGCA_C
Cuts at: 3593 4749 3593
Size: 1156 4380
ApoI r'AATT_y
Cuts at: 1493 2845 3071 3223 5332 1493
Size: 1352 226 152 2109 1697
Fragments arranged by size:
2109 1697 1352 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4090 160
Size: 872 3058 1606
Fragments arranged by size:
3058 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
AvaI C'yCGr_G
Cuts at: 1870 1943 2034 2163 2556 2861 1870
Size: 73 91 129 393 305 4545
Fragments arranged by size:
4545 393 305 129 91 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2509 2542 2509
Size: 33 5503
BamHI G'GATC_C
Cuts at: 2866 3233 2866
Size: 367 5169
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2052 2142 2489 2552 2857
Size: 325 217 627 265 90 347 63 305
Cuts at: 2857 4312 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 347 325 305 265 217
90 63
BanII G_rGCy'C
Cuts at: 1391 2035 2170 2273 2471 2699 2772 2855 1391
Size: 644 135 103 198 228 73 83 4072
Fragments arranged by size:
4072 644 228 198 135 103 83 73
BbeI G_GCGC'C
Cuts at: 2556 2556
Size: 5536
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4565
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2186 2220 3727 3761 1903
Size: 34 249 34 1507 34 3678
Fragments arranged by size:
3678 1507 249 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3428 4638 3428
Size: 1210 4326
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5536
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 2585 4147
Size: 107 122 71 631 605 367 546 1562
Cuts at: 4147 136
Size: 1525
Fragments arranged by size:
1562 1525 631 605 546 367 122 107
71
BglII A'GATC_T
Cuts at: 5531 5531
Size: 5536
BlpI GC'TnA_GC
Cuts at: 2953 2953
Size: 5536
Bme1580I G_kGCm'C
Cuts at: 1790 2035 2273 3597 4753 1790
Size: 245 238 1324 1156 2573
Fragments arranged by size:
2573 1324 1156 245 238
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5536
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3267 4225 442
Size: 416 53 83 399 24 1850 958 1753
Fragments arranged by size:
1850 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2832 1056
Size: 1776 3760
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 4196 1239
Size: 863 2094 2579
Fragments arranged by size:
2579 2094 863
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2591 3661 4526 4824 2591
Size: 1070 865 298 3303
Fragments arranged by size:
3303 1070 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4199 882
Size: 617 2700 2219
Fragments arranged by size:
2700 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3327 493
Size: 711 2123 2702
Fragments arranged by size:
2702 2123 711
BsaBI GATnn'nnATC
Cuts at: 5530 5530
Size: 5536
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2042 2553 3343 3725
Size: 53 83 186 936 509 511 790 382
Cuts at: 3725 4360 275
Size: 635 1451
Fragments arranged by size:
1451 936 790 635 511 509 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3969 4367 4641 4788 5199 3969
Size: 398 274 147 411 4306
Fragments arranged by size:
4306 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5311
Fragments arranged by size:
5311 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2303 2606 1186
Size: 1117 303 4116
Fragments arranged by size:
4116 1117 303
BseYI C'CCAG_C
Cuts at: 2062 2824 4739 5235 2062
Size: 762 1915 496 2363
Fragments arranged by size:
2363 1915 762 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2132 2419 2801 1163
Size: 573 396 287 382 3898
Fragments arranged by size:
3898 573 396 382 287
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2102 2277 2986 3747 4775 1540
Size: 275 186 101 175 709 761 1028 2301
Fragments arranged by size:
2301 1028 761 709 275 186 175 101
BsiHKAI G_wGCw'C
Cuts at: 3597 3682 4753 3597
Size: 85 1071 4380
Fragments arranged by size:
4380 1071 85
BsmI GAATG_Cn'
Cuts at: 3047 3140 3047
Size: 93 5443
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2170 2273 2471 2699 2772 2855
Size: 399 245 135 103 198 228 73 83
Cuts at: 2855 3597 3682 4753 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 245 228 198 135
103 85 83 73
BspEI T'CCGG_A
Cuts at: 5199 5199
Size: 5536
BspHI T'CATG_A
Cuts at: 2529 2598 3425 5155 2529
Size: 69 827 1730 2910
Fragments arranged by size:
2910 1730 827 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2040 2647 2891 844
Size: 1196 607 244 3489
Fragments arranged by size:
3489 1196 607 244
BsrBI CCG'CTC
Cuts at: 1394 1823 2081 2176 3423 1394
Size: 429 258 95 1247 3507
Fragments arranged by size:
3507 1247 429 258 95
BsrDI GCAATG_nn'
Cuts at: 65 1248 2574 4031 4205 65
Size: 1183 1326 1457 174 1396
Fragments arranged by size:
1457 1396 1326 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2486 2806 4180 4367 1700
Size: 110 676 320 1374 187 2869
Fragments arranged by size:
2869 1374 676 320 187 110
BsrGI T'GTAC_A
Cuts at: 96 2355 96
Size: 2259 3277
BssHII G'CGCG_C
Cuts at: 1925 2215 1925
Size: 290 5246
BssSI C'ACGA_G
Cuts at: 3596 4608 3596
Size: 1012 4524
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2259 2442 794
Size: 1465 183 3888
Fragments arranged by size:
3888 1465 183
BstBI TT'CG_AA
Cuts at: 4340 4340
Size: 5536
BstEII G'GTnAC_C
Cuts at: 1184 2380 1184
Size: 1196 4340
BstXI CCAn_nnnn'nTGG
Cuts at: 2191 2717 2191
Size: 526 5010
BstYI r'GATC_y
Cuts at: 1972 2866 3233 3618 3635 5087 5173 5185 5531
Size: 894 367 385 17 1452 86 12 346
Cuts at: 5531 1972
Size: 1977
Fragments arranged by size:
1977 1452 894 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2545 2545
Size: 5536
BtgI C'CryG_G
Cuts at: 513 1065 2385 2673 2700 513
Size: 552 1320 288 27 3349
Fragments arranged by size:
3349 1320 552 288 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4797
Fragments arranged by size:
4797 683 56
BtsI GCAGTG_nn'
Cuts at: 3052 3846 3866 3052
Size: 794 20 4722
Fragments arranged by size:
4722 794 20
ClaI AT'CG_AT
Cuts at: 1554 2277 1554
Size: 723 4813
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2557 2592 529
Size: 35 1993 35 3473
Fragments arranged by size:
3473 1993 35 35
DraI TTT'AAA
Cuts at: 2840 2840
Size: 5536
DraIII CAC_nnn'GTG
Cuts at: 2442 2442
Size: 5536
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4543 809
Size: 507 3227 1802
Fragments arranged by size:
3227 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2099 2394 2983
Size: 54 1425 50 275 18 269 295 589
Cuts at: 2983 3872 5501 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 589 295 275 269 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2099 2983 1537
Size: 275 287 884 4090
Fragments arranged by size:
4090 884 287 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2321 3466 1353
Size: 968 1145 3423
Fragments arranged by size:
3423 1145 968
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2659 4123 4497 4643 387
Size: 1064 138 1070 1464 374 146 1280
Fragments arranged by size:
1464 1280 1070 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 3599 4196 4983 5251 1239
Size: 393 470 1497 597 787 268 1524
Fragments arranged by size:
1524 1497 787 597 470 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2031 2066 2031
Size: 35 5501
EcoRI G'AATT_C
Cuts at: 2845 3223 2845
Size: 378 5158
FspI TGC'GCA
Cuts at: 4042 4042
Size: 5536
HaeII r_GCGC'y
Cuts at: 1678 2556 2807 4683 1678
Size: 878 251 1876 2531
Fragments arranged by size:
2531 1876 878 251
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2152 2184 2216 2248 4177
Size: 32 135 32 580 32 32 32 1929
Cuts at: 4177 4209 4251 4283 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1929 580 135 42 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2880 3126 669
Size: 988 281 942 246 3079
Fragments arranged by size:
3079 988 942 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5536
HpaI GTT'AAC
Cuts at: 3126 3126
Size: 5536
KasI G'GCGC_C
Cuts at: 2552 2552
Size: 5536
KpnI G_GTAC'C
Cuts at: 2056 2861 2056
Size: 805 4731
MfeI C'AATT_G
Cuts at: 3135 3135
Size: 5536
MluI A'CGCG_T
Cuts at: 5412 5412
Size: 5536
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4650 4834 1305
Size: 3345 184 2007
Fragments arranged by size:
3345 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2396 10
Size: 54 1425 343 564 3150
Fragments arranged by size:
3150 1425 564 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2189 2351 2777 3494 3853 4012 518
Size: 648 1023 162 426 717 359 159 2042
Fragments arranged by size:
2042 1023 717 648 426 359 162 159
MspA1I CmG'CkG
Cuts at: 1606 2098 2110 2140 2387 2952 3629 4777 5022
Size: 492 12 30 247 565 677 1148 245
Cuts at: 5022 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 565 492 247 245 30
12
NaeI GCC'GGC
Cuts at: 2488 2808 2488
Size: 320 5216
NarI GG'CG_CC
Cuts at: 2553 2553
Size: 5536
NcoI C'CATG_G
Cuts at: 513 1065 2673 2700 513
Size: 552 1608 27 3349
Fragments arranged by size:
3349 1608 552 27
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5536
NgoMIV G'CCGG_C
Cuts at: 2486 2806 2486
Size: 320 5216
NheI G'CTAG_C
Cuts at: 1052 2828 1052
Size: 1776 3760
NotI GC'GGCC_GC
Cuts at: 2099 2983 2099
Size: 884 4652
NspI r_CATG'y
Cuts at: 1132 1229 2356 2503 2894 4439 1132
Size: 97 1127 147 391 1545 2229
Fragments arranged by size:
2229 1545 1127 391 147 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5536
PciI A'CATG_T
Cuts at: 2352 4435 2352
Size: 2083 3453
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5536
PmeI GTTT'AAAC
Cuts at: 2840 2840
Size: 5536
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3605 3637 5118 5150 3605
Size: 32 1481 32 3991
Fragments arranged by size:
3991 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5536
PsiI TTA'TAA
Cuts at: 3106 3106
Size: 5536
PspOMI G'GGCC_C
Cuts at: 2031 2269 2031
Size: 238 5298
PstI C_TGCA'G
Cuts at: 830 2139 2658 2888 830
Size: 1309 519 230 3478
Fragments arranged by size:
3478 1309 519 230
PvuI CG_AT'CG
Cuts at: 2001 2277 2001
Size: 276 5260
SacII CC_GC'GG
Cuts at: 2388 2388
Size: 5536
SalI G'TCGA_C
Cuts at: 1936 2878 1936
Size: 942 4594
SbfI CC_TGCA'GG
Cuts at: 2658 2888 2658
Size: 230 5306
ScaI AGT'ACT
Cuts at: 3784 5496 3784
Size: 1712 3824
SexAI A'CCwGG_T
Cuts at: 2438 2438
Size: 5536
SfcI C'TryA_G
Cuts at: 826 1045 2135 2654 2793 2884 4019 4700 4891
Size: 219 1090 519 139 91 1135 681 191
Cuts at: 4891 826
Size: 1471
Fragments arranged by size:
1471 1135 1090 681 519 219 191 139
91
SfoI GGC'GCC
Cuts at: 2554 2554
Size: 5536
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5536
SmaI CCC'GGG
Cuts at: 1872 2036 2165 2558 2863 1872
Size: 164 129 393 305 4545
Fragments arranged by size:
4545 393 305 164 129
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2606 3640 4541 4803 820
Size: 197 926 663 1034 901 262 1553
Fragments arranged by size:
1553 1034 926 901 663 262 197
SnaBI TAC'GTA
Cuts at: 493 3327 493
Size: 2834 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5536
SphI G_CATG'C
Cuts at: 1132 2503 2894 1132
Size: 1371 391 3774
Fragments arranged by size:
3774 1371 391
SspI AAT'ATT
Cuts at: 5 52 2019 3460 5
Size: 47 1967 1441 2081
Fragments arranged by size:
2081 1967 1441 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5536
StyI C'CwwG_G
Cuts at: 513 1065 2673 2700 2975 513
Size: 552 1608 27 275 3074
Fragments arranged by size:
3074 1608 552 275 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3563 3580 3733 3918 4363 325
Size: 1255 1983 17 153 185 445 1498
Fragments arranged by size:
1983 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2355 2415 3782
Size: 274 80 33 51 1443 378 60 1367
Cuts at: 3782 5494 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1367 378 274 138 80 60
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5496 5528 5496
Size: 32 5504
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5536
XbaI T'CTAG_A
Cuts at: 2872 2872
Size: 5536
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5536
XmaI C'CCGG_G
Cuts at: 1870 2034 2163 2556 2861 1870
Size: 164 129 393 305 4545
Fragments arranged by size:
4545 393 305 164 129
XmnI GAAnn'nnTTC
Cuts at: 3665 3665
Size: 5536
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3344 276
Size: 53 83 186 936 1810 2468
Fragments arranged by size:
2468 1810 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SanDI SapI SfiI SrfI
SwaI XcmI