Restriction map for
pFN21AE5301
(Circular) (Six-base) MAPSORT of: pFN21AE5301.seq Check: 666 from: 1 to: 5485
pFN21AE5301 5485 bp
With 182 enzymes: SgfI *
December 8, 2014 13:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2318 2318
Size: 5485
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3295 278
Size: 53 83 186 936 1759 2468
Fragments arranged by size:
2468 1759 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2828 1937
Size: 891 4594
Acc65I G'GTAC_C
Cuts at: 2806 2806
Size: 5485
AclI AA'CG_TT
Cuts at: 1563 3612 3985 1563
Size: 2049 373 3063
Fragments arranged by size:
3063 2049 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3548 4932 5200 1632
Size: 1916 1384 268 1917
Fragments arranged by size:
1917 1916 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5288
AflIII A'CryG_T
Cuts at: 1203 4384 5361 1203
Size: 3181 977 1327
Fragments arranged by size:
3181 1327 977
AgeI A'CCGG_T
Cuts at: 4316 4316
Size: 5485
AhdI GACnn_n'nnGTC
Cuts at: 4214 4214
Size: 5485
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5485
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5485
AlwNI CAG_nnn'CTG
Cuts at: 3228 4800 3228
Size: 1572 3913
ApaI G_GGCC'C
Cuts at: 2599 2599
Size: 5485
ApaLI G'TGCA_C
Cuts at: 2263 3542 4698 2263
Size: 1279 1156 3050
Fragments arranged by size:
3050 1279 1156
ApoI r'AATT_y
Cuts at: 1493 2357 2794 3020 3172 5281 1493
Size: 864 437 226 152 2109 1697
Fragments arranged by size:
2109 1697 864 437 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4039 160
Size: 872 3007 1606
Fragments arranged by size:
3007 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
AvaI C'yCGr_G
Cuts at: 1870 1943 2102 2810 1870
Size: 73 159 708 4545
Fragments arranged by size:
4545 708 159 73
BamHI G'GATC_C
Cuts at: 2815 3182 2815
Size: 367 5118
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2150 2177 2469 2772 2806
Size: 325 217 627 363 27 292 303 34
Cuts at: 2806 4261 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 363 325 303 292 217
34 27
BanII G_rGCy'C
Cuts at: 1391 2199 2599 2804 1391
Size: 808 400 205 4072
Fragments arranged by size:
4072 808 400 205
BbeI G_GCGC'C
Cuts at: 2154 2154
Size: 5485
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4514
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3676 3710 1903
Size: 34 1739 34 3678
Fragments arranged by size:
3678 1739 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3377 4587 3377
Size: 1210 4275
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5485
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2159 2547 2740
Size: 107 122 71 631 605 487 388 193
Cuts at: 2740 4096 136
Size: 1356 1525
Fragments arranged by size:
1525 1356 631 605 487 388 193 122
107 71
BglII A'GATC_T
Cuts at: 5480 5480
Size: 5485
BlpI GC'TnA_GC
Cuts at: 2902 2902
Size: 5485
Bme1580I G_kGCm'C
Cuts at: 1790 2180 2267 2295 2599 2775 3546 4702 1790
Size: 390 87 28 304 176 771 1156 2573
Fragments arranged by size:
2573 1156 771 390 304 176 87 28
BmgBI CAC'GTC
Cuts at: 1318 2290 1318
Size: 972 4513
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3216 4174 442
Size: 416 53 83 399 24 1799 958 1753
Fragments arranged by size:
1799 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2591 4145 1239
Size: 1352 1554 2579
Fragments arranged by size:
2579 1554 1352
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3610 4475 4773 3610
Size: 865 298 4322
Fragments arranged by size:
4322 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4148 882
Size: 617 2649 2219
Fragments arranged by size:
2649 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3276 493
Size: 711 2072 2702
Fragments arranged by size:
2702 2072 711
BsaBI GATnn'nnATC
Cuts at: 5479 5479
Size: 5485
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2099 2151 3292 3674
Size: 53 83 186 936 566 52 1141 382
Cuts at: 3674 4309 275
Size: 635 1451
Fragments arranged by size:
1451 1141 936 635 566 382 186 83
53 52
BsaWI w'CCGG_w
Cuts at: 3918 4316 4590 4737 5148 3918
Size: 398 274 147 411 4255
Fragments arranged by size:
4255 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2639 2669 1178
Size: 30 165 30 1236 30 3994
Fragments arranged by size:
3994 1236 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 1186
Size: 862 4623
BseYI C'CCAG_C
Cuts at: 2443 4688 5184 2443
Size: 2245 496 2744
Fragments arranged by size:
2744 2245 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2329 2523 1163
Size: 573 593 194 4125
Fragments arranged by size:
4125 593 573 194
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2935 3696 4724 1540
Size: 275 186 934 761 1028 2301
Fragments arranged by size:
2301 1028 934 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2199 2267 2493 3546 3631 4702 2199
Size: 68 226 1053 85 1071 2982
Fragments arranged by size:
2982 1071 1053 226 85 68
BsmI GAATG_Cn'
Cuts at: 2383 2584 2996 3089 2383
Size: 201 412 93 4779
Fragments arranged by size:
4779 412 201 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2180 2199 2267 2295 2493 2599 2775
Size: 399 390 19 68 28 198 106 176
Cuts at: 2775 2804 3546 3631 4702 1391
Size: 29 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 390 198 176 106
85 68 29 28 19
BspEI T'CCGG_A
Cuts at: 5148 5148
Size: 5485
BspHI T'CATG_A
Cuts at: 3374 5104 3374
Size: 1730 3755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2076 2318 2840 844
Size: 1232 242 522 3489
Fragments arranged by size:
3489 1232 522 242
BsrBI CCG'CTC
Cuts at: 1394 1823 3372 1394
Size: 429 1549 3507
Fragments arranged by size:
3507 1549 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3980 4154 65
Size: 1183 2732 174 1396
Fragments arranged by size:
2732 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2040 2061 2684 2708 4129 4316 1700
Size: 110 230 21 623 24 1421 187 2869
Fragments arranged by size:
2869 1421 623 230 187 110 24 21
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5485
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5485
BssSI C'ACGA_G
Cuts at: 3545 4557 3545
Size: 1012 4473
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5485
BstBI TT'CG_AA
Cuts at: 4289 4289
Size: 5485
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5485
BstYI r'GATC_y
Cuts at: 1972 2815 3182 3567 3584 5036 5122 5134 5480
Size: 843 367 385 17 1452 86 12 346
Cuts at: 5480 1972
Size: 1977
Fragments arranged by size:
1977 1452 843 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2055 2145 2172 2295 2319 2367 513
Size: 552 990 90 27 123 24 48 3631
Fragments arranged by size:
3631 990 552 123 90 48 27 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4746
Fragments arranged by size:
4746 683 56
BtsI GCAGTG_nn'
Cuts at: 2445 2729 3001 3795 3815 2445
Size: 284 272 794 20 4115
Fragments arranged by size:
4115 794 284 272 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5485
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5450
DraI TTT'AAA
Cuts at: 2356 2789 2356
Size: 433 5052
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4492 809
Size: 507 3176 1802
Fragments arranged by size:
3176 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2139 2932 3821
Size: 54 1425 50 275 18 309 793 889
Cuts at: 3821 5450 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 793 309 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2932 1537
Size: 275 1120 4090
Fragments arranged by size:
4090 1120 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3415 1353
Size: 2062 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2300 4072 4446 4592 387
Size: 1064 138 711 1772 374 146 1280
Fragments arranged by size:
1772 1280 1064 711 374 146 138
EcoICRI GAG'CTC
Cuts at: 2197 2197
Size: 5485
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2591 3548 4145 4932 5200 1239
Size: 393 959 957 597 787 268 1524
Fragments arranged by size:
1524 959 957 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2030 2030
Size: 5485
EcoO109I rG'GnC_Cy
Cuts at: 2595 2596 2751 2595
Size: 1 155 5329
Fragments arranged by size:
5329 155 1
EcoRI G'AATT_C
Cuts at: 2794 3172 2794
Size: 378 5107
FseI GG_CCGG'CC
Cuts at: 2688 2688
Size: 5485
FspI TGC'GCA
Cuts at: 3991 3991
Size: 5485
HaeII r_GCGC'y
Cuts at: 1678 2132 2154 2226 4632 1678
Size: 454 22 72 2406 2531
Fragments arranged by size:
2531 2406 454 72 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4126 4158 4200 4232 1373
Size: 32 135 32 2554 32 42 32 2626
Fragments arranged by size:
2626 2554 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2829 3075 669
Size: 988 281 891 246 3079
Fragments arranged by size:
3079 988 891 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5485
HpaI GTT'AAC
Cuts at: 3075 3075
Size: 5485
KasI G'GCGC_C
Cuts at: 2150 2150
Size: 5485
KpnI G_GTAC'C
Cuts at: 2810 2810
Size: 5485
MfeI C'AATT_G
Cuts at: 3084 3084
Size: 5485
MluI A'CGCG_T
Cuts at: 5361 5361
Size: 5485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2040 2503 4599 4783 1305
Size: 735 463 2096 184 2007
Fragments arranged by size:
2096 2007 735 463 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3663
Fragments arranged by size:
3663 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2486 3443 3802 3961 518
Size: 648 1320 957 359 159 2042
Fragments arranged by size:
2042 1320 957 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2057 2321 2426 2901 3578 4726 4971 1606
Size: 451 264 105 475 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 475 451 264 245 105
NaeI GCC'GGC
Cuts at: 2063 2686 2710 2063
Size: 623 24 4838
Fragments arranged by size:
4838 623 24
NarI GG'CG_CC
Cuts at: 2151 2151
Size: 5485
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4933
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5485
NgoMIV G'CCGG_C
Cuts at: 2061 2684 2708 2061
Size: 623 24 4838
Fragments arranged by size:
4838 623 24
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5485
NotI GC'GGCC_GC
Cuts at: 2932 2932
Size: 5485
NspI r_CATG'y
Cuts at: 1132 1229 2843 4388 1132
Size: 97 1614 1545 2229
Fragments arranged by size:
2229 1614 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2665 2745 1348
Size: 1317 80 4088
Fragments arranged by size:
4088 1317 80
PciI A'CATG_T
Cuts at: 4384 4384
Size: 5485
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4583
PmeI GTTT'AAAC
Cuts at: 2789 2789
Size: 5485
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3554 3586 5067 5099 3554
Size: 32 1481 32 3940
Fragments arranged by size:
3940 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2751 2751
Size: 5485
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5485
PsiI TTA'TAA
Cuts at: 3055 3055
Size: 5485
PspOMI G'GGCC_C
Cuts at: 2595 2595
Size: 5485
PstI C_TGCA'G
Cuts at: 830 2607 2837 830
Size: 1777 230 3478
Fragments arranged by size:
3478 1777 230
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5485
PvuII CAG'CTG
Cuts at: 2426 2426
Size: 5485
SacI G_AGCT'C
Cuts at: 2199 2199
Size: 5485
SacII CC_GC'GG
Cuts at: 2058 2322 2058
Size: 264 5221
SalI G'TCGA_C
Cuts at: 1936 2827 1936
Size: 891 4594
SanDI GG'GwC_CC
Cuts at: 2751 2751
Size: 5485
SbfI CC_TGCA'GG
Cuts at: 2837 2837
Size: 5485
ScaI AGT'ACT
Cuts at: 2654 3733 5445 2654
Size: 1079 1712 2694
Fragments arranged by size:
2694 1712 1079
SfcI C'TryA_G
Cuts at: 826 1045 2603 2833 3968 4649 4840 826
Size: 219 1558 230 1135 681 191 1471
Fragments arranged by size:
1558 1471 1135 681 230 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2547 2547
Size: 5485
SfoI GGC'GCC
Cuts at: 2152 2152
Size: 5485
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5485
SmaI CCC'GGG
Cuts at: 1872 2812 1872
Size: 940 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3589 4490 4752 820
Size: 197 926 1646 901 262 1553
Fragments arranged by size:
1646 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3276 493
Size: 2783 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5485
SphI G_CATG'C
Cuts at: 1132 2843 1132
Size: 1711 3774
SspI AAT'ATT
Cuts at: 5 52 3409 5
Size: 47 3357 2081
Fragments arranged by size:
3357 2081 47
StuI AGG'CCT
Cuts at: 1517 2550 1517
Size: 1033 4452
StyI C'CwwG_G
Cuts at: 513 1065 2463 2924 513
Size: 552 1398 461 3074
Fragments arranged by size:
3074 1398 552 461
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2179 3512 3529 3682 3867 4312 325
Size: 1255 599 1333 17 153 185 445 1498
Fragments arranged by size:
1498 1333 1255 599 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2652 3731 5443
Size: 274 80 33 51 1443 675 1079 1712
Cuts at: 5443 96
Size: 138
Fragments arranged by size:
1712 1443 1079 675 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5445 5477 5445
Size: 32 5453
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5485
XbaI T'CTAG_A
Cuts at: 2821 2821
Size: 5485
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5485
XmaI C'CCGG_G
Cuts at: 1870 2810 1870
Size: 940 4545
XmnI GAAnn'nnTTC
Cuts at: 3614 3614
Size: 5485
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3293 276
Size: 53 83 186 936 1759 2468
Fragments arranged by size:
2468 1759 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FspAI NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SapI SexAI SrfI SwaI XcmI