Restriction map for
pFN21AE5295
(Circular) (Six-base) MAPSORT of: pFN21AE5295.seq Check: 938 from: 1 to: 5317
pFN21AE5295 5317 bp
With 182 enzymes: SgfI *
January 30, 2014 17:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2482 2482
Size: 5317
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3127 278
Size: 53 83 186 936 1591 2468
Fragments arranged by size:
2468 1591 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2660 1937
Size: 723 4594
Acc65I G'GTAC_C
Cuts at: 2638 2638
Size: 5317
AclI AA'CG_TT
Cuts at: 1563 3444 3817 1563
Size: 1881 373 3063
Fragments arranged by size:
3063 1881 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2253 2297 3380 4764 5032 1632
Size: 621 44 1083 1384 268 1917
Fragments arranged by size:
1917 1384 1083 621 268 44
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5120
AflIII A'CryG_T
Cuts at: 1203 4216 5193 1203
Size: 3013 977 1327
Fragments arranged by size:
3013 1327 977
AgeI A'CCGG_T
Cuts at: 4148 4148
Size: 5317
AhdI GACnn_n'nnGTC
Cuts at: 4046 4046
Size: 5317
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5317
AlwNI CAG_nnn'CTG
Cuts at: 3060 4632 3060
Size: 1572 3745
ApaLI G'TGCA_C
Cuts at: 3374 4530 3374
Size: 1156 4161
ApoI r'AATT_y
Cuts at: 1493 2626 2852 3004 5113 1493
Size: 1133 226 152 2109 1697
Fragments arranged by size:
2109 1697 1133 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3871 160
Size: 872 2839 1606
Fragments arranged by size:
2839 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5317
AvaI C'yCGr_G
Cuts at: 1870 1943 2397 2642 1870
Size: 73 454 245 4545
Fragments arranged by size:
4545 454 245 73
BamHI G'GATC_C
Cuts at: 2514 2647 3014 2514
Size: 133 367 4817
Fragments arranged by size:
4817 367 133
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2638 4093 618
Size: 325 217 627 851 1455 1842
Fragments arranged by size:
1842 1455 851 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2396 2636 1391
Size: 1005 240 4072
Fragments arranged by size:
4072 1005 240
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4346
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2032 2066 3508 3542 1903
Size: 34 95 34 1442 34 3678
Fragments arranged by size:
3678 1442 95 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3209 4419 3209
Size: 1210 4107
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5317
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2188 3928 136
Size: 107 122 71 631 605 516 1740 1525
Fragments arranged by size:
1740 1525 631 605 516 122 107 71
BglII A'GATC_T
Cuts at: 5312 5312
Size: 5317
BlpI GC'TnA_GC
Cuts at: 2192 2734 2192
Size: 542 4775
Bme1580I G_kGCm'C
Cuts at: 1790 2118 3378 4534 1790
Size: 328 1260 1156 2573
Fragments arranged by size:
2573 1260 1156 328
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5317
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2126 2373 3048
Size: 416 53 83 399 24 709 247 675
Cuts at: 3048 4006 442
Size: 958 1753
Fragments arranged by size:
1753 958 709 675 416 399 247 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2567 1056
Size: 1511 3806
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2355 2355
Size: 5317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3977 1239
Size: 2738 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2350 3442 4307 4605 2350
Size: 1092 865 298 3062
Fragments arranged by size:
3062 1092 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3980 882
Size: 617 2481 2219
Fragments arranged by size:
2481 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3108 493
Size: 711 1904 2702
Fragments arranged by size:
2702 1904 711
BsaBI GATnn'nnATC
Cuts at: 5311 5311
Size: 5317
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3124 3506 4141 275
Size: 53 83 186 936 1591 382 635 1451
Fragments arranged by size:
1591 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3750 4148 4422 4569 4980 3750
Size: 398 274 147 411 4087
Fragments arranged by size:
4087 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5092
Fragments arranged by size:
5092 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2138 2587 2603 1186
Size: 952 449 16 3900
Fragments arranged by size:
3900 952 449 16
BseYI C'CCAG_C
Cuts at: 2522 2540 4520 5016 2522
Size: 18 1980 496 2823
Fragments arranged by size:
2823 1980 496 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4744
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2767 3528 4556 1540
Size: 275 186 766 761 1028 2301
Fragments arranged by size:
2301 1028 766 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2386 3378 3463 4534 2386
Size: 992 85 1071 3169
Fragments arranged by size:
3169 1071 992 85
BsmI GAATG_Cn'
Cuts at: 2828 2921 2828
Size: 93 5224
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2386 2396 2636 3378 3463 4534
Size: 399 328 268 10 240 742 85 1071
Cuts at: 4534 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 328 268 240 85
10
BspEI T'CCGG_A
Cuts at: 4980 4980
Size: 5317
BspHI T'CATG_A
Cuts at: 3206 4936 3206
Size: 1730 3587
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2482 2672 844
Size: 1638 190 3489
Fragments arranged by size:
3489 1638 190
BsrBI CCG'CTC
Cuts at: 1394 1823 3204 1394
Size: 429 1381 3507
Fragments arranged by size:
3507 1381 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3812 3986 65
Size: 1183 2564 174 1396
Fragments arranged by size:
2564 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2015 3961 4148 1700
Size: 110 205 1946 187 2869
Fragments arranged by size:
2869 1946 205 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5317
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5317
BssSI C'ACGA_G
Cuts at: 3377 4389 3377
Size: 1012 4305
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5317
BstBI TT'CG_AA
Cuts at: 4121 4121
Size: 5317
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5317
BstYI r'GATC_y
Cuts at: 1972 2514 2647 3014 3399 3416 4868 4954 4966
Size: 542 133 367 385 17 1452 86 12
Cuts at: 4966 5312 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 542 385 367 346 133 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2199 2199
Size: 5317
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3826
Fragments arranged by size:
3826 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4578
Fragments arranged by size:
4578 683 56
BtsI GCAGTG_nn'
Cuts at: 2205 2833 3627 3647 2205
Size: 628 794 20 3875
Fragments arranged by size:
3875 794 628 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5317
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5282
DraI TTT'AAA
Cuts at: 2621 2621
Size: 5317
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4324 809
Size: 507 3008 1802
Fragments arranged by size:
3008 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2764 3653 5282
Size: 54 1425 50 275 18 934 889 1629
Cuts at: 5282 8
Size: 43
Fragments arranged by size:
1629 1425 934 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2764 1537
Size: 275 952 4090
Fragments arranged by size:
4090 952 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2285 2355 2493 2580 3247 1353
Size: 932 70 138 87 667 3423
Fragments arranged by size:
3423 932 667 138 87 70
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3904 4278 4424 387
Size: 1064 138 2315 374 146 1280
Fragments arranged by size:
2315 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2253 2297 3380 3977 4764 5032 1239
Size: 393 621 44 1083 597 787 268 1524
Fragments arranged by size:
1524 1083 787 621 597 393 268 44
EcoNI CCTnn'n_nnAGG
Cuts at: 2332 2332
Size: 5317
EcoO109I rG'GnC_Cy
Cuts at: 2076 2128 2076
Size: 52 5265
EcoRI G'AATT_C
Cuts at: 2626 3004 2626
Size: 378 4939
FspI TGC'GCA
Cuts at: 3823 3823
Size: 5317
HaeII r_GCGC'y
Cuts at: 1678 2582 4464 1678
Size: 904 1882 2531
Fragments arranged by size:
2531 1882 904
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2336 2368 3958 3990 4032
Size: 32 135 32 764 32 1590 32 42
Cuts at: 4032 4064 1373
Size: 32 2626
Fragments arranged by size:
2626 1590 764 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2058 2661 2907 669
Size: 988 281 120 603 246 3079
Fragments arranged by size:
3079 988 603 281 246 120
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5317
HpaI GTT'AAC
Cuts at: 2907 2907
Size: 5317
KpnI G_GTAC'C
Cuts at: 2642 2642
Size: 5317
MfeI C'AATT_G
Cuts at: 2916 2916
Size: 5317
MluI A'CGCG_T
Cuts at: 5193 5193
Size: 5317
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4431 4615 1305
Size: 3126 184 2007
Fragments arranged by size:
3126 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3495
Fragments arranged by size:
3495 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3275 3634 3793 518
Size: 648 2109 359 159 2042
Fragments arranged by size:
2109 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2092 2414 2438 2512 2733 3410 4558 4803
Size: 486 322 24 74 221 677 1148 245
Cuts at: 4803 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 486 322 245 221 74
24
NaeI GCC'GGC
Cuts at: 2017 2017
Size: 5317
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3826
Fragments arranged by size:
3826 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5317
NgoMIV G'CCGG_C
Cuts at: 2015 2015
Size: 5317
NheI G'CTAG_C
Cuts at: 1052 2563 1052
Size: 1511 3806
NotI GC'GGCC_GC
Cuts at: 2764 2764
Size: 5317
NspI r_CATG'y
Cuts at: 1132 1229 2675 4220 1132
Size: 97 1446 1545 2229
Fragments arranged by size:
2229 1545 1446 97
PasI CC'CwG_GG
Cuts at: 1348 2186 1348
Size: 838 4479
PciI A'CATG_T
Cuts at: 4216 4216
Size: 5317
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5317
PmeI GTTT'AAAC
Cuts at: 2621 2621
Size: 5317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2235 2267 3386 3418 4899 4931 2235
Size: 32 1119 32 1481 32 2621
Fragments arranged by size:
2621 1481 1119 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2076 2128 2076
Size: 52 5265
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5317
PsiI TTA'TAA
Cuts at: 2887 2887
Size: 5317
PstI C_TGCA'G
Cuts at: 830 2508 2669 830
Size: 1678 161 3478
Fragments arranged by size:
3478 1678 161
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5317
PvuII CAG'CTG
Cuts at: 2414 2438 2414
Size: 24 5293
SalI G'TCGA_C
Cuts at: 1936 2659 1936
Size: 723 4594
SapI GCTCTTCn'nnn_
Cuts at: 2493 2493
Size: 5317
SbfI CC_TGCA'GG
Cuts at: 2669 2669
Size: 5317
ScaI AGT'ACT
Cuts at: 3565 5277 3565
Size: 1712 3605
SfcI C'TryA_G
Cuts at: 826 1045 2504 2665 3800 4481 4672 826
Size: 219 1459 161 1135 681 191 1471
Fragments arranged by size:
1471 1459 1135 681 219 191 161
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5317
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5317
SmaI CCC'GGG
Cuts at: 1872 2644 1872
Size: 772 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2329 3421 4322 4584 820
Size: 197 926 386 1092 901 262 1553
Fragments arranged by size:
1553 1092 926 901 386 262 197
SnaBI TAC'GTA
Cuts at: 493 3108 493
Size: 2615 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5317
SphI G_CATG'C
Cuts at: 1132 2675 1132
Size: 1543 3774
SspI AAT'ATT
Cuts at: 5 52 3241 5
Size: 47 3189 2081
Fragments arranged by size:
3189 2081 47
StuI AGG'CCT
Cuts at: 1517 2230 2250 2327 2613 1517
Size: 713 20 77 286 4221
Fragments arranged by size:
4221 713 286 77 20
StyI C'CwwG_G
Cuts at: 513 1065 2004 2756 513
Size: 552 939 752 3074
Fragments arranged by size:
3074 939 752 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3344 3361 3514 3699 4144 325
Size: 1255 1764 17 153 185 445 1498
Fragments arranged by size:
1764 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3563 5275 96
Size: 274 80 33 51 1443 1586 1712 138
Fragments arranged by size:
1712 1586 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5277 5309 5277
Size: 32 5285
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5317
XbaI T'CTAG_A
Cuts at: 2653 2653
Size: 5317
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5317
XmaI C'CCGG_G
Cuts at: 1870 2642 1870
Size: 772 4545
XmnI GAAnn'nnTTC
Cuts at: 2159 3446 2159
Size: 1287 4030
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3125 276
Size: 53 83 186 936 1591 2468
Fragments arranged by size:
2468 1591 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI