Restriction map for
pFN21AE5290
(Circular) (Six-base) MAPSORT of: pFN21AE5290.seq Check: 1435 from: 1 to: 5461
pFN21AE5290 5461 bp
With 182 enzymes: SgfI *
January 30, 2014 17:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3271 278
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2613 2804 1937
Size: 676 191 4594
Fragments arranged by size:
4594 676 191
Acc65I G'GTAC_C
Cuts at: 2782 2782
Size: 5461
AclI AA'CG_TT
Cuts at: 1563 3588 3961 1563
Size: 2025 373 3063
Fragments arranged by size:
3063 2025 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2105 2567 2608 3524 4908 5176 1632
Size: 473 462 41 916 1384 268 1917
Fragments arranged by size:
1917 1384 916 473 462 268 41
AfeI AGC'GCT
Cuts at: 2565 2565
Size: 5461
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5264
AflIII A'CryG_T
Cuts at: 1203 4360 5337 1203
Size: 3157 977 1327
Fragments arranged by size:
3157 1327 977
AgeI A'CCGG_T
Cuts at: 4292 4292
Size: 5461
AhdI GACnn_n'nnGTC
Cuts at: 4190 4190
Size: 5461
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5461
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2535 2567 1184
Size: 1351 32 4078
Fragments arranged by size:
4078 1351 32
AlwNI CAG_nnn'CTG
Cuts at: 2095 2271 3204 4776 2095
Size: 176 933 1572 2780
Fragments arranged by size:
2780 1572 933 176
ApaLI G'TGCA_C
Cuts at: 3518 4674 3518
Size: 1156 4305
ApoI r'AATT_y
Cuts at: 1493 2770 2996 3148 5257 1493
Size: 1277 226 152 2109 1697
Fragments arranged by size:
2109 1697 1277 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4015 160
Size: 872 2983 1606
Fragments arranged by size:
2983 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
AvaI C'yCGr_G
Cuts at: 1870 1943 2786 1870
Size: 73 843 4545
Fragments arranged by size:
4545 843 73
BamHI G'GATC_C
Cuts at: 2791 3158 2791
Size: 367 5094
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2782 4237 618
Size: 325 217 627 995 1455 1842
Fragments arranged by size:
1842 1455 995 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2040 2133 2191 2780 1391
Size: 649 93 58 589 4072
Fragments arranged by size:
4072 649 589 93 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4490
BbvCI CC'TCA_GC
Cuts at: 2284 2284
Size: 5461
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3652 3686 1903
Size: 34 1715 34 3678
Fragments arranged by size:
3678 1715 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3353 4563 3353
Size: 1210 4251
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5461
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2156 2225 2586
Size: 107 122 71 631 605 484 69 361
Cuts at: 2586 4072 136
Size: 1486 1525
Fragments arranged by size:
1525 1486 631 605 484 361 122 107
71 69
BglII A'GATC_T
Cuts at: 5456 5456
Size: 5461
BlpI GC'TnA_GC
Cuts at: 2127 2447 2633 2878 2127
Size: 320 186 245 4710
Fragments arranged by size:
4710 320 245 186
Bme1580I G_kGCm'C
Cuts at: 1790 2210 2747 3522 4678 1790
Size: 420 537 775 1156 2573
Fragments arranged by size:
2573 1156 775 537 420
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5461
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2534 3192 4150
Size: 416 53 83 399 24 1117 658 958
Cuts at: 4150 442
Size: 1753
Fragments arranged by size:
1753 1117 958 658 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5461
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2669 2723 4121 1239
Size: 878 552 54 1398 2579
Fragments arranged by size:
2579 1398 878 552 54
Bpu10I CC'TnA_GC
Cuts at: 2132 2284 2404 2132
Size: 152 120 5189
Fragments arranged by size:
5189 152 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3586 4451 4749 3586
Size: 865 298 4298
Fragments arranged by size:
4298 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2242 4124 882
Size: 617 743 1882 2219
Fragments arranged by size:
2219 1882 743 617
BsaAI yAC'GTr
Cuts at: 493 1204 2459 3252 493
Size: 711 1255 793 2702
Fragments arranged by size:
2702 1255 793 711
BsaBI GATnn'nnATC
Cuts at: 5455 5455
Size: 5461
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3268 3650 4285 275
Size: 53 83 186 936 1735 382 635 1451
Fragments arranged by size:
1735 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3894 4292 4566 4713 5124 3894
Size: 398 274 147 411 4231
Fragments arranged by size:
4231 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5236
Fragments arranged by size:
5236 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5461
BseYI C'CCAG_C
Cuts at: 2203 2602 4664 5160 2203
Size: 399 2062 496 2504
Fragments arranged by size:
2504 2062 496 399
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2666 1163
Size: 573 930 3958
Fragments arranged by size:
3958 930 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2668 2674 2911 3672 4700 1540
Size: 275 186 667 6 237 761 1028 2301
Fragments arranged by size:
2301 1028 761 667 275 237 186 6
BsiHKAI G_wGCw'C
Cuts at: 2133 2191 2448 3522 3607 4678 2133
Size: 58 257 1074 85 1071 2916
Fragments arranged by size:
2916 1074 1071 257 85 58
BsmI GAATG_Cn'
Cuts at: 2972 3065 2972
Size: 93 5368
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2133 2191 2210 2448 2747 2780
Size: 399 250 93 58 19 238 299 33
Cuts at: 2780 3522 3607 4678 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 299 250 238 93
85 58 33 19
BspEI T'CCGG_A
Cuts at: 5124 5124
Size: 5461
BspHI T'CATG_A
Cuts at: 3350 5080 3350
Size: 1730 3731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2816 844
Size: 1972 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3348 1394
Size: 429 1525 3507
Fragments arranged by size:
3507 1525 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2428 3956 4130 65
Size: 1183 1180 1528 174 1396
Fragments arranged by size:
1528 1396 1183 1180 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2491 4105 4292 1700
Size: 110 681 1614 187 2869
Fragments arranged by size:
2869 1614 681 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5461
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5461
BssSI C'ACGA_G
Cuts at: 2411 3521 4533 2411
Size: 1110 1012 3339
Fragments arranged by size:
3339 1110 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5461
BstBI TT'CG_AA
Cuts at: 4265 4265
Size: 5461
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5461
BstXI CCAn_nnnn'nTGG
Cuts at: 2231 2463 2647 2231
Size: 232 184 5045
Fragments arranged by size:
5045 232 184
BstYI r'GATC_y
Cuts at: 1972 2791 3158 3543 3560 5012 5098 5110 5456
Size: 819 367 385 17 1452 86 12 346
Cuts at: 5456 1972
Size: 1977
Fragments arranged by size:
1977 1452 819 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2230 2668 513
Size: 552 939 226 438 3306
Fragments arranged by size:
3306 939 552 438 226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4722
Fragments arranged by size:
4722 683 56
BtsI GCAGTG_nn'
Cuts at: 2208 2977 3771 3791 2208
Size: 769 794 20 3878
Fragments arranged by size:
3878 794 769 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5461
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5426
DraI TTT'AAA
Cuts at: 2765 2765
Size: 5461
DraIII CAC_nnn'GTG
Cuts at: 2689 2751 2689
Size: 62 5399
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4468 809
Size: 507 3152 1802
Fragments arranged by size:
3152 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2217 2329 2415
Size: 54 1425 50 275 18 387 112 86
Cuts at: 2415 2493 2538 2665 2671 2908 3797 5426 8
Size: 78 45 127 6 237 889 1629 43
Fragments arranged by size:
1629 1425 889 387 275 237 127 112
86 78 54 50 45 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2665 2671 2908 1537
Size: 275 853 6 237 4090
Fragments arranged by size:
4090 853 275 237 6
EarI CTCTTCn'nnn_
Cuts at: 1353 2190 2300 3391 1353
Size: 837 110 1091 3423
Fragments arranged by size:
3423 1091 837 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2370 4048 4422 4568 387
Size: 1064 138 781 1678 374 146 1280
Fragments arranged by size:
1678 1280 1064 781 374 146 138
EcoICRI GAG'CTC
Cuts at: 2131 2189 2131
Size: 58 5403
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2117 2567 2608 2669 2723 3524
Size: 393 473 12 450 41 61 54 801
Cuts at: 3524 4121 4908 5176 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 801 787 597 473 450 393 268
61 54 41 12
EcoO109I rG'GnC_Cy
Cuts at: 2019 2092 2102 2169 2268 2275 2578 2730 2019
Size: 73 10 67 99 7 303 152 4750
Fragments arranged by size:
4750 303 152 99 73 67 10 7
EcoRI G'AATT_C
Cuts at: 2770 3148 2770
Size: 378 5083
FspI TGC'GCA
Cuts at: 3967 3967
Size: 5461
HaeII r_GCGC'y
Cuts at: 1678 2567 4608 1678
Size: 889 2041 2531
Fragments arranged by size:
2531 2041 889
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2022 2054 2267 2299 4102
Size: 32 135 32 450 32 213 32 1803
Cuts at: 4102 4134 4176 4208 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1803 450 213 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2805 3051 669
Size: 988 281 867 246 3079
Fragments arranged by size:
3079 988 867 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5461
HpaI GTT'AAC
Cuts at: 3051 3051
Size: 5461
KpnI G_GTAC'C
Cuts at: 2786 2786
Size: 5461
MfeI C'AATT_G
Cuts at: 3060 3060
Size: 5461
MluI A'CGCG_T
Cuts at: 5337 5337
Size: 5461
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4575 4759 1305
Size: 3270 184 2007
Fragments arranged by size:
3270 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2219 2331 2417 2540 10
Size: 54 1425 343 387 112 86 123 2931
Fragments arranged by size:
2931 1425 387 343 123 112 86 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3419 3778 3937 518
Size: 648 2253 359 159 2042
Fragments arranged by size:
2253 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2485 2670 2877 3554 4702 4947 1606
Size: 879 185 207 677 1148 245 2120
Fragments arranged by size:
2120 1148 879 677 245 207 185
NcoI C'CATG_G
Cuts at: 513 1065 2004 2230 513
Size: 552 939 226 3744
Fragments arranged by size:
3744 939 552 226
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5461
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5461
NotI GC'GGCC_GC
Cuts at: 2671 2908 2671
Size: 237 5224
NspI r_CATG'y
Cuts at: 1132 1229 2115 2819 4364 1132
Size: 97 886 704 1545 2229
Fragments arranged by size:
2229 1545 886 704 97
PasI CC'CwG_GG
Cuts at: 1348 2065 2245 2386 1348
Size: 717 180 141 4423
Fragments arranged by size:
4423 717 180 141
PciI A'CATG_T
Cuts at: 4360 4360
Size: 5461
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2059 2095 1108
Size: 951 36 4474
Fragments arranged by size:
4474 951 36
PmeI GTTT'AAAC
Cuts at: 2765 2765
Size: 5461
PmlI CAC'GTG
Cuts at: 2459 2459
Size: 5461
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3530 3562 5043 5075 3530
Size: 32 1481 32 3916
Fragments arranged by size:
3916 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2268 2275 2730 2268
Size: 7 455 4999
Fragments arranged by size:
4999 455 7
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5461
PsiI TTA'TAA
Cuts at: 3031 3031
Size: 5461
PstI C_TGCA'G
Cuts at: 830 2514 2597 2813 830
Size: 1684 83 216 3478
Fragments arranged by size:
3478 1684 216 83
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5461
PvuII CAG'CTG
Cuts at: 2485 2485
Size: 5461
SacI G_AGCT'C
Cuts at: 2133 2191 2133
Size: 58 5403
SacII CC_GC'GG
Cuts at: 2671 2671
Size: 5461
SalI G'TCGA_C
Cuts at: 1936 2803 1936
Size: 867 4594
SapI GCTCTTCn'nnn_
Cuts at: 2190 2300 2190
Size: 110 5351
SbfI CC_TGCA'GG
Cuts at: 2813 2813
Size: 5461
ScaI AGT'ACT
Cuts at: 3709 5421 3709
Size: 1712 3749
SfcI C'TryA_G
Cuts at: 826 1045 2510 2593 2809 3944 4625 4816 826
Size: 219 1465 83 216 1135 681 191 1471
Fragments arranged by size:
1471 1465 1135 681 219 216 191 83
SfiI GGCCn_nnn'nGGCC
Cuts at: 2225 2225
Size: 5461
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5461
SmaI CCC'GGG
Cuts at: 1872 2788 1872
Size: 916 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3565 4466 4728 820
Size: 197 926 1622 901 262 1553
Fragments arranged by size:
1622 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3252 493
Size: 2759 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5461
SphI G_CATG'C
Cuts at: 1132 2115 2819 1132
Size: 983 704 3774
Fragments arranged by size:
3774 983 704
SspI AAT'ATT
Cuts at: 5 52 3385 5
Size: 47 3333 2081
Fragments arranged by size:
3333 2081 47
StuI AGG'CCT
Cuts at: 1517 2363 1517
Size: 846 4615
StyI C'CwwG_G
Cuts at: 513 1065 2004 2230 2900 513
Size: 552 939 226 670 3074
Fragments arranged by size:
3074 939 670 552 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2237 3488 3505 3658 3843 4288 325
Size: 1255 657 1251 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1251 657 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3707 5419 96
Size: 274 80 33 51 1443 1730 1712 138
Fragments arranged by size:
1730 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2520 2552 5421 5453 2520
Size: 32 2869 32 2528
Fragments arranged by size:
2869 2528 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5461
XbaI T'CTAG_A
Cuts at: 2797 2797
Size: 5461
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5461
XmaI C'CCGG_G
Cuts at: 1870 2786 1870
Size: 916 4545
XmnI GAAnn'nnTTC
Cuts at: 3590 3590
Size: 5461
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3269 276
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PspOMI PspXI PsrI RsrII SanDI SexAI
SfoI SrfI SwaI XcmI