Restriction map for
pFN21AE5281
(Circular) (Six-base) MAPSORT of: pFN21AE5281.seq Check: 9313 from: 1 to: 5458
pFN21AE5281 5458 bp
With 182 enzymes: SgfI *
January 30, 2014 17:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2329 2329
Size: 5458
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3268 278
Size: 53 83 186 936 1732 2468
Fragments arranged by size:
2468 1732 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2801 1937
Size: 864 4594
Acc65I G'GTAC_C
Cuts at: 2779 2779
Size: 5458
AclI AA'CG_TT
Cuts at: 1563 3585 3958 1563
Size: 2022 373 3063
Fragments arranged by size:
3063 2022 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2134 2302 3521 4905 5173 1632
Size: 502 168 1219 1384 268 1917
Fragments arranged by size:
1917 1384 1219 502 268 168
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5261
AflIII A'CryG_T
Cuts at: 1203 4357 5334 1203
Size: 3154 977 1327
Fragments arranged by size:
3154 1327 977
AgeI A'CCGG_T
Cuts at: 4289 4289
Size: 5458
AhdI GACnn_n'nnGTC
Cuts at: 4187 4187
Size: 5458
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5458
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5458
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2679 2711 2679
Size: 32 5426
AlwNI CAG_nnn'CTG
Cuts at: 2653 3201 4773 2653
Size: 548 1572 3338
Fragments arranged by size:
3338 1572 548
ApaI G_GGCC'C
Cuts at: 2110 2110
Size: 5458
ApaLI G'TGCA_C
Cuts at: 3515 4671 3515
Size: 1156 4302
ApoI r'AATT_y
Cuts at: 1493 2767 2993 3145 5254 1493
Size: 1274 226 152 2109 1697
Fragments arranged by size:
2109 1697 1274 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4012 160
Size: 872 2980 1606
Fragments arranged by size:
2980 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
AvaI C'yCGr_G
Cuts at: 1870 1943 2103 2607 2783 1870
Size: 73 160 504 176 4545
Fragments arranged by size:
4545 504 176 160 73
BamHI G'GATC_C
Cuts at: 2788 3155 2788
Size: 367 5091
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2779 4234 618
Size: 325 217 627 992 1455 1842
Fragments arranged by size:
1842 1455 992 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2110 2130 2170 2744 2777 1391
Size: 719 20 40 574 33 4072
Fragments arranged by size:
4072 719 574 40 33 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4487
BbvCI CC'TCA_GC
Cuts at: 2131 2355 2131
Size: 224 5234
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3649 3683 1903
Size: 34 1712 34 3678
Fragments arranged by size:
3678 1712 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3350 4560 3350
Size: 1210 4248
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5458
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2099 2739 4069
Size: 107 122 71 631 605 427 640 1330
Cuts at: 4069 136
Size: 1525
Fragments arranged by size:
1525 1330 640 631 605 427 122 107
71
BglII A'GATC_T
Cuts at: 5453 5453
Size: 5458
BlpI GC'TnA_GC
Cuts at: 2875 2875
Size: 5458
Bme1580I G_kGCm'C
Cuts at: 1790 2110 3519 4675 1790
Size: 320 1409 1156 2573
Fragments arranged by size:
2573 1409 1156 320
BmgBI CAC'GTC
Cuts at: 1318 2037 1318
Size: 719 4739
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3189 4147 442
Size: 416 53 83 399 24 1772 958 1753
Fragments arranged by size:
1772 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2733 4118 1239
Size: 899 595 1385 2579
Fragments arranged by size:
2579 1385 899 595
Bpu10I CC'TnA_GC
Cuts at: 2060 2131 2198 2277 2355 2060
Size: 71 67 79 78 5163
Fragments arranged by size:
5163 79 78 71 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3583 4448 4746 3583
Size: 865 298 4295
Fragments arranged by size:
4295 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4121 882
Size: 617 2622 2219
Fragments arranged by size:
2622 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3249 493
Size: 711 2045 2702
Fragments arranged by size:
2702 2045 711
BsaBI GATnn'nnATC
Cuts at: 5452 5452
Size: 5458
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3265 3647 4282 275
Size: 53 83 186 936 1732 382 635 1451
Fragments arranged by size:
1732 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3891 4289 4563 4710 5121 3891
Size: 398 274 147 411 4228
Fragments arranged by size:
4228 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2679 2709 1178
Size: 30 165 30 1276 30 3927
Fragments arranged by size:
3927 1276 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5458
BseYI C'CCAG_C
Cuts at: 4661 5157 4661
Size: 496 4962
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2314 1163
Size: 573 578 4307
Fragments arranged by size:
4307 578 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2908 3669 4697 1540
Size: 275 186 907 761 1028 2301
Fragments arranged by size:
2301 1028 907 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3519 3604 4675 3519
Size: 85 1071 4302
Fragments arranged by size:
4302 1071 85
BsmI GAATG_Cn'
Cuts at: 2969 3062 2969
Size: 93 5365
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2110 2130 2170 2744 2777 3519 3604
Size: 399 320 20 40 574 33 742 85
Cuts at: 3604 4675 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 574 399 320 85 40
33 20
BspEI T'CCGG_A
Cuts at: 5121 5121
Size: 5458
BspHI T'CATG_A
Cuts at: 3347 5077 3347
Size: 1730 3728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2329 2813 844
Size: 1485 484 3489
Fragments arranged by size:
3489 1485 484
BsrBI CCG'CTC
Cuts at: 1394 1823 3345 1394
Size: 429 1522 3507
Fragments arranged by size:
3507 1522 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3953 4127 65
Size: 1183 2705 174 1396
Fragments arranged by size:
2705 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2013 4102 4289 1700
Size: 110 203 2089 187 2869
Fragments arranged by size:
2869 2089 203 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5458
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5458
BssSI C'ACGA_G
Cuts at: 3518 4530 3518
Size: 1012 4446
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5458
BstBI TT'CG_AA
Cuts at: 4262 4262
Size: 5458
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5458
BstYI r'GATC_y
Cuts at: 1972 2788 3155 3540 3557 5009 5095 5107 5453
Size: 816 367 385 17 1452 86 12 346
Cuts at: 5453 1972
Size: 1977
Fragments arranged by size:
1977 1452 816 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2077 2366 2077
Size: 289 5169
BtgI C'CryG_G
Cuts at: 513 1065 2004 2110 2203 2661 513
Size: 552 939 106 93 458 3310
Fragments arranged by size:
3310 939 552 458 106 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2278 487
Size: 683 56 1052 3667
Fragments arranged by size:
3667 1052 683 56
BtsI GCAGTG_nn'
Cuts at: 2406 2974 3768 3788 2406
Size: 568 794 20 4076
Fragments arranged by size:
4076 794 568 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5458
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5423
DraI TTT'AAA
Cuts at: 2762 2762
Size: 5458
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2663 4465 809
Size: 507 1347 1802 1802
Fragments arranged by size:
1802 1802 1347 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2905 3794 5423
Size: 54 1425 50 275 18 1075 889 1629
Cuts at: 5423 8
Size: 43
Fragments arranged by size:
1629 1425 1075 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2905 1537
Size: 275 1093 4090
Fragments arranged by size:
4090 1093 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3388 1353
Size: 2035 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2057 4045 4419 4565 387
Size: 1064 138 468 1988 374 146 1280
Fragments arranged by size:
1988 1280 1064 468 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2134 2138 2302 2733 3521 4118 4905
Size: 393 502 4 164 431 788 597 787
Cuts at: 4905 5173 1239
Size: 268 1524
Fragments arranged by size:
1524 788 787 597 502 431 393 268
164 4
EcoO109I rG'GnC_Cy
Cuts at: 2443 2603 2443
Size: 160 5298
EcoRI G'AATT_C
Cuts at: 2767 3145 2767
Size: 378 5080
FspI TGC'GCA
Cuts at: 3964 3964
Size: 5458
HaeII r_GCGC'y
Cuts at: 1678 4605 1678
Size: 2927 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4099 4131 4173 4205 1373
Size: 32 135 32 2527 32 42 32 2626
Fragments arranged by size:
2626 2527 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2802 3048 669
Size: 988 281 864 246 3079
Fragments arranged by size:
3079 988 864 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5458
HpaI GTT'AAC
Cuts at: 3048 3048
Size: 5458
KpnI G_GTAC'C
Cuts at: 2783 2783
Size: 5458
MfeI C'AATT_G
Cuts at: 3057 3057
Size: 5458
MluI A'CGCG_T
Cuts at: 5334 5334
Size: 5458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2325 2433 4572 4756 1305
Size: 1020 108 2139 184 2007
Fragments arranged by size:
2139 2007 1020 184 108
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3636
Fragments arranged by size:
3636 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3416 3775 3934 518
Size: 648 2250 359 159 2042
Fragments arranged by size:
2250 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2874 3551 4699 4944 1606
Size: 1268 677 1148 245 2120
Fragments arranged by size:
2120 1268 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2110 513
Size: 552 939 106 3861
Fragments arranged by size:
3861 939 552 106
NdeI CA'TA_TG
Cuts at: 387 2547 387
Size: 2160 3298
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5458
NotI GC'GGCC_GC
Cuts at: 2905 2905
Size: 5458
NspI r_CATG'y
Cuts at: 1132 1229 2623 2816 4361 1132
Size: 97 1394 193 1545 2229
Fragments arranged by size:
2229 1545 1394 193 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5458
PciI A'CATG_T
Cuts at: 4357 4357
Size: 5458
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2116 1108
Size: 1008 4450
PfoI T'CCnGG_A
Cuts at: 2268 2523 2268
Size: 255 5203
PmeI GTTT'AAAC
Cuts at: 2762 2762
Size: 5458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2679 2711 3527 3559 5040 5072 2679
Size: 32 816 32 1481 32 3065
Fragments arranged by size:
3065 1481 816 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5458
PsiI TTA'TAA
Cuts at: 2310 3028 2310
Size: 718 4740
PspOMI G'GGCC_C
Cuts at: 2106 2106
Size: 5458
PstI C_TGCA'G
Cuts at: 830 2022 2247 2810 830
Size: 1192 225 563 3478
Fragments arranged by size:
3478 1192 563 225
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5458
SalI G'TCGA_C
Cuts at: 1936 2800 1936
Size: 864 4594
SbfI CC_TGCA'GG
Cuts at: 2810 2810
Size: 5458
ScaI AGT'ACT
Cuts at: 3706 5418 3706
Size: 1712 3746
SfcI C'TryA_G
Cuts at: 826 1045 2018 2243 2283 2806 3941 4622 4813
Size: 219 973 225 40 523 1135 681 191
Cuts at: 4813 826
Size: 1471
Fragments arranged by size:
1471 1135 973 681 523 225 219 191
40
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5458
SmaI CCC'GGG
Cuts at: 1872 2105 2785 1872
Size: 233 680 4545
Fragments arranged by size:
4545 680 233
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3562 4463 4725 820
Size: 197 926 1619 901 262 1553
Fragments arranged by size:
1619 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3249 493
Size: 2756 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5458
SphI G_CATG'C
Cuts at: 1132 2623 2816 1132
Size: 1491 193 3774
Fragments arranged by size:
3774 1491 193
SrfI GCCC'GGGC
Cuts at: 2105 2105
Size: 5458
SspI AAT'ATT
Cuts at: 5 52 3382 5
Size: 47 3330 2081
Fragments arranged by size:
3330 2081 47
StuI AGG'CCT
Cuts at: 1517 2087 2531 1517
Size: 570 444 4444
Fragments arranged by size:
4444 570 444
StyI C'CwwG_G
Cuts at: 513 1065 2004 2097 2110 2446 2897 513
Size: 552 939 93 13 336 451 3074
Fragments arranged by size:
3074 939 552 451 336 93 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2433 3485 3502 3655 3840 4285 325
Size: 1255 853 1052 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1052 853 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3704 5416 96
Size: 274 80 33 51 1443 1727 1712 138
Fragments arranged by size:
1727 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5418 5450 5418
Size: 32 5426
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5458
XbaI T'CTAG_A
Cuts at: 2794 2794
Size: 5458
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5458
XmaI C'CCGG_G
Cuts at: 1870 2103 2783 1870
Size: 233 680 4545
Fragments arranged by size:
4545 680 233
XmnI GAAnn'nnTTC
Cuts at: 3587 3587
Size: 5458
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3266 276
Size: 53 83 186 936 1732 2468
Fragments arranged by size:
2468 1732 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SwaI XcmI