Restriction map for
pFN21AE5280
(Circular) (Six-base) MAPSORT of: pFN21AE5280.seq Check: 1355 from: 1 to: 5449
pFN21AE5280 5449 bp
With 182 enzymes: SgfI *
January 30, 2014 17:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2426 2426
Size: 5449
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3259 278
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2792 1937
Size: 855 4594
Acc65I G'GTAC_C
Cuts at: 2247 2770 2247
Size: 523 4926
AclI AA'CG_TT
Cuts at: 1563 3576 3949 1563
Size: 2013 373 3063
Fragments arranged by size:
3063 2013 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3512 4896 5164 1632
Size: 1880 1384 268 1917
Fragments arranged by size:
1917 1880 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5252
AflIII A'CryG_T
Cuts at: 1203 2106 4348 5325 1203
Size: 903 2242 977 1327
Fragments arranged by size:
2242 1327 977 903
AgeI A'CCGG_T
Cuts at: 4280 4280
Size: 5449
AhdI GACnn_n'nnGTC
Cuts at: 4178 4178
Size: 5449
AleI CACnn'nnGTG
Cuts at: 1166 2427 1166
Size: 1261 4188
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5449
AlwNI CAG_nnn'CTG
Cuts at: 3192 4764 3192
Size: 1572 3877
ApaI G_GGCC'C
Cuts at: 2595 2595
Size: 5449
ApaLI G'TGCA_C
Cuts at: 3506 4662 3506
Size: 1156 4293
ApoI r'AATT_y
Cuts at: 1493 2758 2984 3136 5245 1493
Size: 1265 226 152 2109 1697
Fragments arranged by size:
2109 1697 1265 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4003 160
Size: 872 2971 1606
Fragments arranged by size:
2971 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
AvaI C'yCGr_G
Cuts at: 1870 1943 2328 2774 1870
Size: 73 385 446 4545
Fragments arranged by size:
4545 446 385 73
BamHI G'GATC_C
Cuts at: 2779 3146 2779
Size: 367 5082
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2247 2770 4225 618
Size: 325 217 627 460 523 1455 1842
Fragments arranged by size:
1842 1455 627 523 460 325 217
BanII G_rGCy'C
Cuts at: 1391 2326 2595 2768 1391
Size: 935 269 173 4072
Fragments arranged by size:
4072 935 269 173
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2214 928
Size: 971 315 4163
Fragments arranged by size:
4163 971 315
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2461 2495 3640 3674 1903
Size: 34 524 34 1145 34 3678
Fragments arranged by size:
3678 1145 524 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3341 4551 3341
Size: 1210 4239
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5449
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2298 4060 136
Size: 107 122 71 631 605 626 1762 1525
Fragments arranged by size:
1762 1525 631 626 605 122 107 71
BglII A'GATC_T
Cuts at: 2546 2615 2732 5444 2546
Size: 69 117 2712 2551
Fragments arranged by size:
2712 2551 117 69
BlpI GC'TnA_GC
Cuts at: 2866 2866
Size: 5449
Bme1580I G_kGCm'C
Cuts at: 1790 2595 3510 4666 1790
Size: 805 915 1156 2573
Fragments arranged by size:
2573 1156 915 805
BmgBI CAC'GTC
Cuts at: 1318 2107 1318
Size: 789 4660
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3180 4138 442
Size: 416 53 83 399 24 1763 958 1753
Fragments arranged by size:
1763 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2061 4109 1239
Size: 822 2048 2579
Fragments arranged by size:
2579 2048 822
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2129 3574 4439 4737 2129
Size: 1445 865 298 2841
Fragments arranged by size:
2841 1445 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4112 882
Size: 617 2613 2219
Fragments arranged by size:
2613 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2062 3240 493
Size: 711 858 1178 2702
Fragments arranged by size:
2702 1178 858 711
BsaBI GATnn'nnATC
Cuts at: 2620 5443 2620
Size: 2823 2626
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3256 3638 4273 275
Size: 53 83 186 936 1723 382 635 1451
Fragments arranged by size:
1723 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3882 4280 4554 4701 5112 3882
Size: 398 274 147 411 4219
Fragments arranged by size:
4219 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2004 2034 2662 2692 1178
Size: 30 165 30 601 30 628 30 3935
Fragments arranged by size:
3935 628 601 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5449
BseYI C'CCAG_C
Cuts at: 4652 5148 4652
Size: 496 4953
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4876
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2281 2899 3660 4688 1540
Size: 275 186 280 618 761 1028 2301
Fragments arranged by size:
2301 1028 761 618 280 275 186
BsiHKAI G_wGCw'C
Cuts at: 2326 3510 3595 4666 2326
Size: 1184 85 1071 3109
Fragments arranged by size:
3109 1184 1071 85
BsmI GAATG_Cn'
Cuts at: 2960 3053 2960
Size: 93 5356
BsmBI CGTCTCn'nnnn_
Cuts at: 2193 2193
Size: 5449
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2326 2595 2768 3510 3595 4666 1391
Size: 399 536 269 173 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 536 399 269 173 85
BspEI T'CCGG_A
Cuts at: 5112 5112
Size: 5449
BspHI T'CATG_A
Cuts at: 2551 3338 5068 2551
Size: 787 1730 2932
Fragments arranged by size:
2932 1730 787
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2426 2804 844
Size: 1582 378 3489
Fragments arranged by size:
3489 1582 378
BsrBI CCG'CTC
Cuts at: 1394 1823 3336 1394
Size: 429 1513 3507
Fragments arranged by size:
3507 1513 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3944 4118 65
Size: 1183 2696 174 1396
Fragments arranged by size:
2696 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4093 4280 1700
Size: 110 2283 187 2869
Fragments arranged by size:
2869 2283 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5449
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5449
BssSI C'ACGA_G
Cuts at: 3509 4521 3509
Size: 1012 4437
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5449
BstBI TT'CG_AA
Cuts at: 2521 4253 2521
Size: 1732 3717
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5449
BstYI r'GATC_y
Cuts at: 1972 2546 2615 2732 2779 3146 3531 3548 5000
Size: 574 69 117 47 367 385 17 1452
Cuts at: 5000 5086 5098 5444 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 574 385 367 346 117 86
69 47 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2155 2281 513
Size: 552 1090 126 3681
Fragments arranged by size:
3681 1090 552 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4710
Fragments arranged by size:
4710 683 56
BtsI GCAGTG_nn'
Cuts at: 2965 3759 3779 2965
Size: 794 20 4635
Fragments arranged by size:
4635 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5449
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5414
DraI TTT'AAA
Cuts at: 2415 2753 2415
Size: 338 5111
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4456 809
Size: 507 3140 1802
Fragments arranged by size:
3140 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2278 2896 3785
Size: 54 1425 50 275 18 448 618 889
Cuts at: 3785 5414 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 618 448 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2278 2896 1537
Size: 275 466 618 4090
Fragments arranged by size:
4090 618 466 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3379 1353
Size: 2026 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2334 4036 4410 4556 387
Size: 1064 138 745 1702 374 146 1280
Fragments arranged by size:
1702 1280 1064 745 374 146 138
EcoICRI GAG'CTC
Cuts at: 2324 2324
Size: 5449
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2061 3512 4109 4896 5164 1239
Size: 393 429 1451 597 787 268 1524
Fragments arranged by size:
1524 1451 787 597 429 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2191 2191
Size: 5449
EcoRI G'AATT_C
Cuts at: 2758 3136 2758
Size: 378 5071
FspI TGC'GCA
Cuts at: 3955 3955
Size: 5449
HaeII r_GCGC'y
Cuts at: 1678 2127 4596 1678
Size: 449 2469 2531
Fragments arranged by size:
2531 2469 449
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2361 2393 4090 4122 4164
Size: 32 135 32 789 32 1697 32 42
Cuts at: 4164 4196 1373
Size: 32 2626
Fragments arranged by size:
2626 1697 789 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2793 3039 669
Size: 988 281 855 246 3079
Fragments arranged by size:
3079 988 855 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5449
HpaI GTT'AAC
Cuts at: 3039 3039
Size: 5449
KpnI G_GTAC'C
Cuts at: 2251 2774 2251
Size: 523 4926
MfeI C'AATT_G
Cuts at: 3048 3048
Size: 5449
MluI A'CGCG_T
Cuts at: 5325 5325
Size: 5449
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4563 4747 1305
Size: 3258 184 2007
Fragments arranged by size:
3258 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3627
Fragments arranged by size:
3627 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2060 2427 3407 3766 3925 518
Size: 648 894 367 980 359 159 2042
Fragments arranged by size:
2042 980 894 648 367 359 159
MspA1I CmG'CkG
Cuts at: 1606 2157 2865 3542 4690 4935 1606
Size: 551 708 677 1148 245 2120
Fragments arranged by size:
2120 1148 708 677 551 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4897
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5449
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5449
NotI GC'GGCC_GC
Cuts at: 2896 2896
Size: 5449
NruI TCG'CGA
Cuts at: 2226 2226
Size: 5449
NspI r_CATG'y
Cuts at: 1132 1229 2428 2807 4352 1132
Size: 97 1199 379 1545 2229
Fragments arranged by size:
2229 1545 1199 379 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5449
PciI A'CATG_T
Cuts at: 4348 4348
Size: 5449
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2609 1108
Size: 1501 3948
PmeI GTTT'AAAC
Cuts at: 2753 2753
Size: 5449
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2093 2125 3518 3550 5031 5063 2093
Size: 32 1393 32 1481 32 2479
Fragments arranged by size:
2479 1481 1393 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2191 2191
Size: 5449
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5449
PsiI TTA'TAA
Cuts at: 3019 3019
Size: 5449
PspOMI G'GGCC_C
Cuts at: 2591 2591
Size: 5449
PstI C_TGCA'G
Cuts at: 830 2801 830
Size: 1971 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5449
SacI G_AGCT'C
Cuts at: 2326 2326
Size: 5449
SacII CC_GC'GG
Cuts at: 2158 2158
Size: 5449
SalI G'TCGA_C
Cuts at: 1936 2791 1936
Size: 855 4594
SbfI CC_TGCA'GG
Cuts at: 2801 2801
Size: 5449
ScaI AGT'ACT
Cuts at: 3697 5409 3697
Size: 1712 3737
SfcI C'TryA_G
Cuts at: 826 1045 2797 3932 4613 4804 826
Size: 219 1752 1135 681 191 1471
Fragments arranged by size:
1752 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5449
SmaI CCC'GGG
Cuts at: 1872 2776 1872
Size: 904 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2144 3553 4454 4716 820
Size: 197 926 201 1409 901 262 1553
Fragments arranged by size:
1553 1409 926 901 262 201 197
SnaBI TAC'GTA
Cuts at: 493 3240 493
Size: 2747 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5449
SphI G_CATG'C
Cuts at: 1132 2807 1132
Size: 1675 3774
SspI AAT'ATT
Cuts at: 5 52 3373 5
Size: 47 3321 2081
Fragments arranged by size:
3321 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5449
StyI C'CwwG_G
Cuts at: 513 1065 2091 2687 2888 513
Size: 552 1026 596 201 3074
Fragments arranged by size:
3074 1026 596 552 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3476 3493 3646 3831 4276 325
Size: 1255 1896 17 153 185 445 1498
Fragments arranged by size:
1896 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3695 5407 96
Size: 274 80 33 51 1443 1718 1712 138
Fragments arranged by size:
1718 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2004 2036 5409 5441 2004
Size: 32 3373 32 2012
Fragments arranged by size:
3373 2012 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5449
XbaI T'CTAG_A
Cuts at: 2785 2785
Size: 5449
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2400 2400
Size: 5449
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5449
XmaI C'CCGG_G
Cuts at: 1870 2774 1870
Size: 904 4545
XmnI GAAnn'nnTTC
Cuts at: 3578 3578
Size: 5449
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3257 276
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NruI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacI SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII
SanDI SapI SexAI SfiI SfoI SrfI SwaI