Restriction map for
pFN21AE5271
(Circular) (Six-base) MAPSORT of: pFN21AE5271.seq Check: 5806 from: 1 to: 5359
pFN21AE5271 5359 bp
With 182 enzymes: SgfI *
January 30, 2014 17:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3169 278
Size: 53 83 186 936 1633 2468
Fragments arranged by size:
2468 1633 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2702 1937
Size: 765 4594
Acc65I G'GTAC_C
Cuts at: 2680 2680
Size: 5359
AclI AA'CG_TT
Cuts at: 1563 3486 3859 1563
Size: 1923 373 3063
Fragments arranged by size:
3063 1923 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2177 2237 3422 4806 5074 1632
Size: 545 60 1185 1384 268 1917
Fragments arranged by size:
1917 1384 1185 545 268 60
AfeI AGC'GCT
Cuts at: 2096 2096
Size: 5359
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5162
AflIII A'CryG_T
Cuts at: 1203 4258 5235 1203
Size: 3055 977 1327
Fragments arranged by size:
3055 1327 977
AgeI A'CCGG_T
Cuts at: 4190 4190
Size: 5359
AhdI GACnn_n'nnGTC
Cuts at: 2632 4088 2632
Size: 1456 3903
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5359
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5359
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2283 2315 2283
Size: 32 5327
AlwNI CAG_nnn'CTG
Cuts at: 2212 3102 4674 2212
Size: 890 1572 2897
Fragments arranged by size:
2897 1572 890
ApaLI G'TGCA_C
Cuts at: 3416 4572 3416
Size: 1156 4203
ApoI r'AATT_y
Cuts at: 1493 2668 2894 3046 5155 1493
Size: 1175 226 152 2109 1697
Fragments arranged by size:
2109 1697 1175 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3913 160
Size: 872 2881 1606
Fragments arranged by size:
2881 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5359
AvaI C'yCGr_G
Cuts at: 1870 1943 2318 2489 2684 1870
Size: 73 375 171 195 4545
Fragments arranged by size:
4545 375 195 171 73
BamHI G'GATC_C
Cuts at: 2689 3056 2689
Size: 367 4992
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2680 4135 618
Size: 325 217 627 893 1455 1842
Fragments arranged by size:
1842 1455 893 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2678 1391
Size: 1287 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2415 928
Size: 971 516 3872
Fragments arranged by size:
3872 971 516
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3550 3584 1903
Size: 34 1613 34 3678
Fragments arranged by size:
3678 1613 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3251 4461 3251
Size: 1210 4149
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5359
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2147 3970 136
Size: 107 122 71 631 605 475 1823 1525
Fragments arranged by size:
1823 1525 631 605 475 122 107 71
BglII A'GATC_T
Cuts at: 2562 5354 2562
Size: 2792 2567
BlpI GC'TnA_GC
Cuts at: 2776 2776
Size: 5359
Bme1580I G_kGCm'C
Cuts at: 1790 3420 4576 1790
Size: 1630 1156 2573
Fragments arranged by size:
2573 1630 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5359
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3090 4048 442
Size: 416 53 83 399 24 1673 958 1753
Fragments arranged by size:
1753 1673 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2111 2351 2560 4019 1239
Size: 872 240 209 1459 2579
Fragments arranged by size:
2579 1459 872 240 209
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2033 3484 4349 4647 2033
Size: 1451 865 298 2745
Fragments arranged by size:
2745 1451 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2327 4022 882
Size: 617 828 1695 2219
Fragments arranged by size:
2219 1695 828 617
BsaAI yAC'GTr
Cuts at: 493 1204 3150 493
Size: 711 1946 2702
Fragments arranged by size:
2702 1946 711
BsaBI GATnn'nnATC
Cuts at: 2567 5353 2567
Size: 2786 2573
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3166 3548 4183 275
Size: 53 83 186 936 1633 382 635 1451
Fragments arranged by size:
1633 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3792 4190 4464 4611 5022 3792
Size: 398 274 147 411 4129
Fragments arranged by size:
4129 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5134
Fragments arranged by size:
5134 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2641 1186
Size: 1455 3904
BseYI C'CCAG_C
Cuts at: 2176 4562 5058 2176
Size: 2386 496 2477
Fragments arranged by size:
2477 2386 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4786
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2809 3570 4598 1540
Size: 275 186 808 761 1028 2301
Fragments arranged by size:
2301 1028 808 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3420 3505 4576 3420
Size: 85 1071 4203
Fragments arranged by size:
4203 1071 85
BsmI GAATG_Cn'
Cuts at: 2870 2963 2870
Size: 93 5266
BsmBI CGTCTCn'nnnn_
Cuts at: 2099 2099
Size: 5359
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2678 3420 3505 4576 1391
Size: 399 888 742 85 1071 2174
Fragments arranged by size:
2174 1071 888 742 399 85
BspEI T'CCGG_A
Cuts at: 5022 5022
Size: 5359
BspHI T'CATG_A
Cuts at: 3248 4978 3248
Size: 1730 3629
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2334 2345 2714 844
Size: 1490 11 369 3489
Fragments arranged by size:
3489 1490 369 11
BsrBI CCG'CTC
Cuts at: 1394 1823 3246 1394
Size: 429 1423 3507
Fragments arranged by size:
3507 1423 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3854 4028 65
Size: 1183 2606 174 1396
Fragments arranged by size:
2606 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4003 4190 1700
Size: 110 2193 187 2869
Fragments arranged by size:
2869 2193 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5359
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5359
BssSI C'ACGA_G
Cuts at: 3419 4431 3419
Size: 1012 4347
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5359
BstBI TT'CG_AA
Cuts at: 4163 4163
Size: 5359
BstEII G'GTnAC_C
Cuts at: 1184 2197 1184
Size: 1013 4346
BstXI CCAn_nnnn'nTGG
Cuts at: 2644 2644
Size: 5359
BstYI r'GATC_y
Cuts at: 1972 2208 2494 2562 2689 3056 3441 3458 4910
Size: 236 286 68 127 367 385 17 1452
Cuts at: 4910 4996 5008 5354 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 286 236 127
86 68 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2193 513
Size: 552 939 189 3679
Fragments arranged by size:
3679 939 552 189
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2024 487
Size: 683 56 798 3822
Fragments arranged by size:
3822 798 683 56
BtsI GCAGTG_nn'
Cuts at: 2875 3669 3689 2875
Size: 794 20 4545
Fragments arranged by size:
4545 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5359
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5324
DraI TTT'AAA
Cuts at: 2663 2663
Size: 5359
DraIII CAC_nnn'GTG
Cuts at: 2256 2256
Size: 5359
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4366 809
Size: 507 3050 1802
Fragments arranged by size:
3050 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2082 2294 2478
Size: 54 1425 50 275 18 252 212 184
Cuts at: 2478 2806 3695 5324 8
Size: 328 889 1629 43
Fragments arranged by size:
1629 1425 889 328 275 252 212 184
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2806 1537
Size: 275 994 4090
Fragments arranged by size:
4090 994 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2577 3289 1353
Size: 1224 712 3423
Fragments arranged by size:
3423 1224 712
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3946 4320 4466 387
Size: 1064 138 2357 374 146 1280
Fragments arranged by size:
2357 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2177 2237 2351 2560 3422 4019
Size: 393 479 66 60 114 209 862 597
Cuts at: 4019 4806 5074 1239
Size: 787 268 1524
Fragments arranged by size:
1524 862 787 597 479 393 268 209
114 66 60
EcoNI CCTnn'n_nnAGG
Cuts at: 2216 2216
Size: 5359
EcoO109I rG'GnC_Cy
Cuts at: 2221 2323 2221
Size: 102 5257
EcoRI G'AATT_C
Cuts at: 2668 3046 2668
Size: 378 4981
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2638 2638
Size: 5359
FspI TGC'GCA
Cuts at: 3865 3865
Size: 5359
HaeII r_GCGC'y
Cuts at: 1678 2098 4506 1678
Size: 420 2408 2531
Fragments arranged by size:
2531 2408 420
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2618 2650 4000 4032 4074
Size: 32 135 32 1046 32 1350 32 42
Cuts at: 4074 4106 1373
Size: 32 2626
Fragments arranged by size:
2626 1350 1046 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2703 2949 669
Size: 988 281 765 246 3079
Fragments arranged by size:
3079 988 765 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5359
HpaI GTT'AAC
Cuts at: 2949 2949
Size: 5359
KpnI G_GTAC'C
Cuts at: 2684 2684
Size: 5359
MfeI C'AATT_G
Cuts at: 2958 2958
Size: 5359
MluI A'CGCG_T
Cuts at: 5235 5235
Size: 5359
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2326 2481 4473 4657 1305
Size: 1021 155 1992 184 2007
Fragments arranged by size:
2007 1992 1021 184 155
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2084 10
Size: 54 1425 343 252 3285
Fragments arranged by size:
3285 1425 343 252 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3317 3676 3835 518
Size: 648 843 1308 359 159 2042
Fragments arranged by size:
2042 1308 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2469 2775 3452 4600 4845 1606
Size: 863 306 677 1148 245 2120
Fragments arranged by size:
2120 1148 863 677 306 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3868
Fragments arranged by size:
3868 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5359
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5359
NotI GC'GGCC_GC
Cuts at: 2806 2806
Size: 5359
NspI r_CATG'y
Cuts at: 1132 1229 2717 4262 1132
Size: 97 1488 1545 2229
Fragments arranged by size:
2229 1545 1488 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5359
PciI A'CATG_T
Cuts at: 4258 4258
Size: 5359
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2440 1108
Size: 1332 4027
PmeI GTTT'AAAC
Cuts at: 2663 2663
Size: 5359
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3428 3460 4941 4973 3428
Size: 32 1481 32 3814
Fragments arranged by size:
3814 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2323 2323
Size: 5359
PshAI GACnn'nnGTC
Cuts at: 1613 2195 1613
Size: 582 4777
PsiI TTA'TAA
Cuts at: 2929 2929
Size: 5359
PstI C_TGCA'G
Cuts at: 830 2711 830
Size: 1881 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5359
SalI G'TCGA_C
Cuts at: 1936 2701 1936
Size: 765 4594
SbfI CC_TGCA'GG
Cuts at: 2711 2711
Size: 5359
ScaI AGT'ACT
Cuts at: 3607 5319 3607
Size: 1712 3647
SfcI C'TryA_G
Cuts at: 826 1045 2572 2707 3842 4523 4714 826
Size: 219 1527 135 1135 681 191 1471
Fragments arranged by size:
1527 1471 1135 681 219 191 135
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5359
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5359
SmaI CCC'GGG
Cuts at: 1872 2686 1872
Size: 814 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2048 3463 4364 4626 820
Size: 197 926 105 1415 901 262 1553
Fragments arranged by size:
1553 1415 926 901 262 197 105
SnaBI TAC'GTA
Cuts at: 493 3150 493
Size: 2657 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5359
SphI G_CATG'C
Cuts at: 1132 2717 1132
Size: 1585 3774
SspI AAT'ATT
Cuts at: 5 52 3283 5
Size: 47 3231 2081
Fragments arranged by size:
3231 2081 47
StuI AGG'CCT
Cuts at: 1517 2351 1517
Size: 834 4525
StyI C'CwwG_G
Cuts at: 513 1065 2004 2115 2798 513
Size: 552 939 111 683 3074
Fragments arranged by size:
3074 939 683 552 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3386 3403 3556 3741 4186 325
Size: 1255 1806 17 153 185 445 1498
Fragments arranged by size:
1806 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3605 5317 96
Size: 274 80 33 51 1443 1628 1712 138
Fragments arranged by size:
1712 1628 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5319 5351 5319
Size: 32 5327
Tth111I GACn'n_nGTC
Cuts at: 1316 2414 1316
Size: 1098 4261
XbaI T'CTAG_A
Cuts at: 2695 2695
Size: 5359
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2605 2605
Size: 5359
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5359
XmaI C'CCGG_G
Cuts at: 1870 2684 1870
Size: 814 4545
XmnI GAAnn'nnTTC
Cuts at: 3488 3488
Size: 5359
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3167 276
Size: 53 83 186 936 1633 2468
Fragments arranged by size:
2468 1633 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI