Restriction map for
pFN21AE5269
(Circular) (Six-base) MAPSORT of: pFN21AE5269.seq Check: 3937 from: 1 to: 5299
pFN21AE5269 5299 bp
With 182 enzymes: SgfI *
January 30, 2014 17:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3109 278
Size: 53 83 186 936 1573 2468
Fragments arranged by size:
2468 1573 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2642 1937
Size: 705 4594
Acc65I G'GTAC_C
Cuts at: 2620 2620
Size: 5299
AclI AA'CG_TT
Cuts at: 1563 2270 3426 3799 1563
Size: 707 1156 373 3063
Fragments arranged by size:
3063 1156 707 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2282 3362 4746 5014 1632
Size: 650 1080 1384 268 1917
Fragments arranged by size:
1917 1384 1080 650 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5102
AflIII A'CryG_T
Cuts at: 1203 4198 5175 1203
Size: 2995 977 1327
Fragments arranged by size:
2995 1327 977
AgeI A'CCGG_T
Cuts at: 4130 4130
Size: 5299
AhdI GACnn_n'nnGTC
Cuts at: 4028 4028
Size: 5299
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5299
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5299
AlwNI CAG_nnn'CTG
Cuts at: 2203 3042 4614 2203
Size: 839 1572 2888
Fragments arranged by size:
2888 1572 839
ApaLI G'TGCA_C
Cuts at: 2154 3356 4512 2154
Size: 1202 1156 2941
Fragments arranged by size:
2941 1202 1156
ApoI r'AATT_y
Cuts at: 1493 2608 2834 2986 5095 1493
Size: 1115 226 152 2109 1697
Fragments arranged by size:
2109 1697 1115 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3853 160
Size: 872 2821 1606
Fragments arranged by size:
2821 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5299
AvaI C'yCGr_G
Cuts at: 1870 1943 2624 1870
Size: 73 681 4545
Fragments arranged by size:
4545 681 73
BamHI G'GATC_C
Cuts at: 2629 2996 2629
Size: 367 4932
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2086 2582 2620 4075 618
Size: 325 217 627 299 496 38 1455 1842
Fragments arranged by size:
1842 1455 627 496 325 299 217 38
BanII G_rGCy'C
Cuts at: 1391 2259 2618 1391
Size: 868 359 4072
Fragments arranged by size:
4072 868 359
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4328
BbvCI CC'TCA_GC
Cuts at: 2541 2541
Size: 5299
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2458 2492 3490 3524 1903
Size: 34 50 34 437 34 998 34 3678
Fragments arranged by size:
3678 998 437 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3191 4401 3191
Size: 1210 4089
BclI T'GATC_A
Cuts at: 1548 2439 1548
Size: 891 4408
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2348 2570 3910
Size: 107 122 71 631 605 676 222 1340
Cuts at: 3910 136
Size: 1525
Fragments arranged by size:
1525 1340 676 631 605 222 122 107
71
BglII A'GATC_T
Cuts at: 5294 5294
Size: 5299
BlpI GC'TnA_GC
Cuts at: 2716 2716
Size: 5299
Bme1580I G_kGCm'C
Cuts at: 1790 2158 3360 4516 1790
Size: 368 1202 1156 2573
Fragments arranged by size:
2573 1202 1156 368
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5299
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3030 3988 442
Size: 416 53 83 399 24 1613 958 1753
Fragments arranged by size:
1753 1613 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2588 2615 3959 1239
Size: 1349 27 1344 2579
Fragments arranged by size:
2579 1349 1344 27
Bpu10I CC'TnA_GC
Cuts at: 2541 2541
Size: 5299
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3424 4289 4587 3424
Size: 865 298 4136
Fragments arranged by size:
4136 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3962 882
Size: 617 2463 2219
Fragments arranged by size:
2463 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3090 493
Size: 711 1886 2702
Fragments arranged by size:
2702 1886 711
BsaBI GATnn'nnATC
Cuts at: 5293 5293
Size: 5299
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3106 3488 4123 275
Size: 53 83 186 936 1573 382 635 1451
Fragments arranged by size:
1573 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3732 4130 4404 4551 4962 3732
Size: 398 274 147 411 4069
Fragments arranged by size:
4069 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5074
Fragments arranged by size:
5074 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2131 1186
Size: 945 4354
BseYI C'CCAG_C
Cuts at: 4502 4998 4502
Size: 496 4803
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4726
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 2749 3510 4538 1540
Size: 275 186 18 730 761 1028 2301
Fragments arranged by size:
2301 1028 761 730 275 186 18
BsiHKAI G_wGCw'C
Cuts at: 2158 2259 3360 3445 4516 2158
Size: 101 1101 85 1071 2941
Fragments arranged by size:
2941 1101 1071 101 85
BsmI GAATG_Cn'
Cuts at: 2810 2903 2810
Size: 93 5206
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2158 2259 2618 3360 3445 4516 1391
Size: 399 368 101 359 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 368 359 101 85
BspEI T'CCGG_A
Cuts at: 4962 4962
Size: 5299
BspHI T'CATG_A
Cuts at: 3188 4918 3188
Size: 1730 3569
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2654 844
Size: 1810 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2021 3186 1394
Size: 429 198 1165 3507
Fragments arranged by size:
3507 1165 429 198
BsrDI GCAATG_nn'
Cuts at: 65 1248 3794 3968 65
Size: 1183 2546 174 1396
Fragments arranged by size:
2546 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2011 3943 4130 1700
Size: 110 201 1932 187 2869
Fragments arranged by size:
2869 1932 201 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5299
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5299
BssSI C'ACGA_G
Cuts at: 3359 4371 3359
Size: 1012 4287
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5299
BstBI TT'CG_AA
Cuts at: 4103 4103
Size: 5299
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5299
BstYI r'GATC_y
Cuts at: 1972 2277 2629 2996 3381 3398 4850 4936 4948
Size: 305 352 367 385 17 1452 86 12
Cuts at: 4948 5294 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 352 346 305 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2421 2586 513
Size: 552 1356 165 3226
Fragments arranged by size:
3226 1356 552 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2200 487
Size: 683 56 974 3586
Fragments arranged by size:
3586 974 683 56
BtsI GCAGTG_nn'
Cuts at: 2815 3609 3629 2815
Size: 794 20 4485
Fragments arranged by size:
4485 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5299
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5264
DraI TTT'AAA
Cuts at: 2603 2603
Size: 5299
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4306 809
Size: 507 2990 1802
Fragments arranged by size:
2990 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2125 2746
Size: 54 1425 50 275 18 186 109 621
Cuts at: 2746 3635 5264 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 621 275 186 109 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2016 2746 1537
Size: 275 204 730 4090
Fragments arranged by size:
4090 730 275 204
EarI CTCTTCn'nnn_
Cuts at: 1353 3229 1353
Size: 1876 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2057 2390 2561 3886 4260 4406
Size: 1064 138 468 333 171 1325 374 146
Cuts at: 4406 387
Size: 1280
Fragments arranged by size:
1325 1280 1064 468 374 333 171 146
138
EcoICRI GAG'CTC
Cuts at: 2257 2257
Size: 5299
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2282 2588 2615 3362 3959 4746 5014
Size: 393 650 306 27 747 597 787 268
Cuts at: 5014 1239
Size: 1524
Fragments arranged by size:
1524 787 747 650 597 393 306 268
27
EcoNI CCTnn'n_nnAGG
Cuts at: 2408 2480 2510 2408
Size: 72 30 5197
Fragments arranged by size:
5197 72 30
EcoO109I rG'GnC_Cy
Cuts at: 2036 2143 2036
Size: 107 5192
EcoRI G'AATT_C
Cuts at: 2608 2986 2608
Size: 378 4921
EcoRV GAT'ATC
Cuts at: 2365 2365
Size: 5299
FspI TGC'GCA
Cuts at: 3805 3805
Size: 5299
HaeII r_GCGC'y
Cuts at: 1678 4446 1678
Size: 2768 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3940 3972 4014 4046 1373
Size: 32 135 32 2368 32 42 32 2626
Fragments arranged by size:
2626 2368 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2643 2889 669
Size: 988 281 705 246 3079
Fragments arranged by size:
3079 988 705 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5299
HpaI GTT'AAC
Cuts at: 2889 2889
Size: 5299
KpnI G_GTAC'C
Cuts at: 2624 2624
Size: 5299
MfeI C'AATT_G
Cuts at: 2898 2898
Size: 5299
MluI A'CGCG_T
Cuts at: 5175 5175
Size: 5299
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4413 4597 1305
Size: 3108 184 2007
Fragments arranged by size:
3108 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3477
Fragments arranged by size:
3477 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3257 3616 3775 518
Size: 648 2091 359 159 2042
Fragments arranged by size:
2091 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2060 2124 2203 2715 3392 4540 4785 1606
Size: 454 64 79 512 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 512 454 245 79 64
NaeI GCC'GGC
Cuts at: 2013 2013
Size: 5299
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4747
NdeI CA'TA_TG
Cuts at: 387 2112 387
Size: 1725 3574
NgoMIV G'CCGG_C
Cuts at: 2011 2011
Size: 5299
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5299
NotI GC'GGCC_GC
Cuts at: 2016 2746 2016
Size: 730 4569
NspI r_CATG'y
Cuts at: 1132 1229 2657 4202 1132
Size: 97 1428 1545 2229
Fragments arranged by size:
2229 1545 1428 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5299
PciI A'CATG_T
Cuts at: 4198 4198
Size: 5299
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5299
PfoI T'CCnGG_A
Cuts at: 2208 2280 2208
Size: 72 5227
PmeI GTTT'AAAC
Cuts at: 2603 2603
Size: 5299
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3368 3400 4881 4913 3368
Size: 32 1481 32 3754
Fragments arranged by size:
3754 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5299
PsiI TTA'TAA
Cuts at: 2869 2869
Size: 5299
PstI C_TGCA'G
Cuts at: 830 2338 2651 830
Size: 1508 313 3478
Fragments arranged by size:
3478 1508 313
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5299
PvuII CAG'CTG
Cuts at: 2203 2203
Size: 5299
SacI G_AGCT'C
Cuts at: 2259 2259
Size: 5299
SalI G'TCGA_C
Cuts at: 1936 2641 1936
Size: 705 4594
SbfI CC_TGCA'GG
Cuts at: 2651 2651
Size: 5299
ScaI AGT'ACT
Cuts at: 3547 5259 3547
Size: 1712 3587
SfcI C'TryA_G
Cuts at: 826 1045 2334 2647 3782 4463 4654 826
Size: 219 1289 313 1135 681 191 1471
Fragments arranged by size:
1471 1289 1135 681 313 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5299
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5299
SmaI CCC'GGG
Cuts at: 1872 2626 1872
Size: 754 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3403 4304 4566 820
Size: 197 926 1460 901 262 1553
Fragments arranged by size:
1553 1460 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3090 493
Size: 2597 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5299
SphI G_CATG'C
Cuts at: 1132 2657 1132
Size: 1525 3774
SspI AAT'ATT
Cuts at: 5 52 3223 5
Size: 47 3171 2081
Fragments arranged by size:
3171 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5299
StyI C'CwwG_G
Cuts at: 513 1065 2044 2175 2464 2738 513
Size: 552 979 131 289 274 3074
Fragments arranged by size:
3074 979 552 289 274 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2294 2423 3326 3343 3496 3681 4126
Size: 1255 714 129 903 17 153 185 445
Cuts at: 4126 325
Size: 1498
Fragments arranged by size:
1498 1255 903 714 445 185 153 129
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3545 5257 96
Size: 274 80 33 51 1443 1568 1712 138
Fragments arranged by size:
1712 1568 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5259 5291 5259
Size: 32 5267
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5299
XbaI T'CTAG_A
Cuts at: 2635 2635
Size: 5299
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5299
XmaI C'CCGG_G
Cuts at: 1870 2624 1870
Size: 754 4545
XmnI GAAnn'nnTTC
Cuts at: 3428 3428
Size: 5299
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3107 276
Size: 53 83 186 936 1573 2468
Fragments arranged by size:
2468 1573 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI