Restriction map for
pFN21AE5268
(Circular) (Six-base) MAPSORT of: pFN21AE5268.seq Check: 6470 from: 1 to: 5308
pFN21AE5268 5308 bp
With 182 enzymes: SgfI *
January 30, 2014 17:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3118 278
Size: 53 83 186 936 1582 2468
Fragments arranged by size:
2468 1582 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2094 2651 1937
Size: 157 557 4594
Fragments arranged by size:
4594 557 157
Acc65I G'GTAC_C
Cuts at: 2629 2629
Size: 5308
AclI AA'CG_TT
Cuts at: 1563 3435 3808 1563
Size: 1872 373 3063
Fragments arranged by size:
3063 1872 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3371 4755 5023 1632
Size: 1739 1384 268 1917
Fragments arranged by size:
1917 1739 1384 268
AfeI AGC'GCT
Cuts at: 2291 2345 2291
Size: 54 5254
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5111
AflIII A'CryG_T
Cuts at: 1203 4207 5184 1203
Size: 3004 977 1327
Fragments arranged by size:
3004 1327 977
AgeI A'CCGG_T
Cuts at: 4139 4139
Size: 5308
AhdI GACnn_n'nnGTC
Cuts at: 4037 4037
Size: 5308
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5308
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5308
AlwNI CAG_nnn'CTG
Cuts at: 3051 4623 3051
Size: 1572 3736
ApaI G_GGCC'C
Cuts at: 2202 2213 2276 2202
Size: 11 63 5234
Fragments arranged by size:
5234 63 11
ApaLI G'TGCA_C
Cuts at: 3365 4521 3365
Size: 1156 4152
ApoI r'AATT_y
Cuts at: 1493 2601 2617 2843 2995 5104 1493
Size: 1108 16 226 152 2109 1697
Fragments arranged by size:
2109 1697 1108 226 152 16
AseI AT'TA_AT
Cuts at: 160 1032 3862 160
Size: 872 2830 1606
Fragments arranged by size:
2830 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5308
AvaI C'yCGr_G
Cuts at: 1870 1943 2213 2277 2633 1870
Size: 73 270 64 356 4545
Fragments arranged by size:
4545 356 270 73 64
BamHI G'GATC_C
Cuts at: 2638 3005 2638
Size: 367 4941
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2407 2629 4084 618
Size: 325 217 627 620 222 1455 1842
Fragments arranged by size:
1842 1455 627 620 325 222 217
BanII G_rGCy'C
Cuts at: 1391 2075 2202 2213 2276 2504 2627 1391
Size: 684 127 11 63 228 123 4072
Fragments arranged by size:
4072 684 228 127 123 63 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2585 928
Size: 971 686 3651
Fragments arranged by size:
3651 971 686
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2204 2238 3499 3533 1903
Size: 34 50 34 183 34 1261 34 3678
Fragments arranged by size:
3678 1261 183 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3200 4410 3200
Size: 1210 4098
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5308
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3919 136
Size: 107 122 71 631 605 2247 1525
Fragments arranged by size:
2247 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5303 5303
Size: 5308
BlpI GC'TnA_GC
Cuts at: 2725 2725
Size: 5308
Bme1580I G_kGCm'C
Cuts at: 1790 2202 2213 2276 2412 3369 4525 1790
Size: 412 11 63 136 957 1156 2573
Fragments arranged by size:
2573 1156 957 412 136 63 11
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5308
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3039 3997 442
Size: 416 53 83 399 24 1622 958 1753
Fragments arranged by size:
1753 1622 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2059 2089 2400 2403 2429 2526 3968 1239
Size: 820 30 311 3 26 97 1442 2579
Fragments arranged by size:
2579 1442 820 311 97 30 26 3
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2458 3433 4298 4596 2458
Size: 975 865 298 3170
Fragments arranged by size:
3170 975 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3971 882
Size: 617 2472 2219
Fragments arranged by size:
2472 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2089 3099 493
Size: 711 885 1010 2702
Fragments arranged by size:
2702 1010 885 711
BsaBI GATnn'nnATC
Cuts at: 5302 5302
Size: 5308
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2133 3115 3497 4132
Size: 53 83 186 936 600 982 382 635
Cuts at: 4132 275
Size: 1451
Fragments arranged by size:
1451 982 936 635 600 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3741 4139 4413 4560 4971 3741
Size: 398 274 147 411 4078
Fragments arranged by size:
4078 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2087 2117 2121 2151 2459
Size: 30 165 30 684 30 4 30 308
Cuts at: 2459 2489 1178
Size: 30 3997
Fragments arranged by size:
3997 684 308 165 30 30 30 30
30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2142 1186
Size: 956 4352
BseYI C'CCAG_C
Cuts at: 2146 2292 2546 4511 5007 2146
Size: 146 254 1965 496 2447
Fragments arranged by size:
2447 1965 496 254 146
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4735
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2758 3519 4547 1540
Size: 275 186 757 761 1028 2301
Fragments arranged by size:
2301 1028 761 757 275 186
BsiHKAI G_wGCw'C
Cuts at: 2303 3369 3454 4525 2303
Size: 1066 85 1071 3086
Fragments arranged by size:
3086 1071 1066 85
BsmI GAATG_Cn'
Cuts at: 2819 2912 2819
Size: 93 5215
BsmBI CGTCTCn'nnnn_
Cuts at: 2124 2210 2124
Size: 86 5222
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2202 2213 2276 2303 2412 2504
Size: 399 285 127 11 63 27 109 92
Cuts at: 2504 2627 3369 3454 4525 1391
Size: 123 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 285 127 123 109
92 85 63 27 11
BspEI T'CCGG_A
Cuts at: 4971 4971
Size: 5308
BspHI T'CATG_A
Cuts at: 3197 4927 3197
Size: 1730 3578
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2663 844
Size: 1819 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3195 1394
Size: 429 1372 3507
Fragments arranged by size:
3507 1372 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3803 3977 65
Size: 1183 2555 174 1396
Fragments arranged by size:
2555 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3952 4139 1700
Size: 110 2142 187 2869
Fragments arranged by size:
2869 2142 187 110
BsrGI T'GTAC_A
Cuts at: 96 2229 96
Size: 2133 3175
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5308
BssSI C'ACGA_G
Cuts at: 3368 4380 3368
Size: 1012 4296
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5308
BstBI TT'CG_AA
Cuts at: 4112 4112
Size: 5308
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5308
BstXI CCAn_nnnn'nTGG
Cuts at: 2495 2495
Size: 5308
BstYI r'GATC_y
Cuts at: 1972 2638 3005 3390 3407 4859 4945 4957 5303
Size: 666 367 385 17 1452 86 12 346
Cuts at: 5303 1972
Size: 1977
Fragments arranged by size:
1977 1452 666 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2205 2205
Size: 5308
BtgI C'CryG_G
Cuts at: 513 1065 2136 513
Size: 552 1071 3685
Fragments arranged by size:
3685 1071 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4569
Fragments arranged by size:
4569 683 56
BtsI GCAGTG_nn'
Cuts at: 2824 3618 3638 2824
Size: 794 20 4494
Fragments arranged by size:
4494 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5308
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5273
DraI TTT'AAA
Cuts at: 2612 2612
Size: 5308
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4315 809
Size: 507 2999 1802
Fragments arranged by size:
2999 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2243 2314 2428
Size: 54 1425 50 275 18 413 71 114
Cuts at: 2428 2755 3644 5273 8
Size: 327 889 1629 43
Fragments arranged by size:
1629 1425 889 413 327 275 114 71
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2755 1537
Size: 275 943 4090
Fragments arranged by size:
4090 943 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3238 1353
Size: 1885 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2072 3895 4269 4415 387
Size: 1064 138 483 1823 374 146 1280
Fragments arranged by size:
1823 1280 1064 483 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2059 2089 2400 2403 2429 2526 3371
Size: 393 427 30 311 3 26 97 845
Cuts at: 3371 3968 4755 5023 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 845 787 597 427 393 311 268
97 30 26 3
EcoO109I rG'GnC_Cy
Cuts at: 2198 2199 2209 2210 2272 2273 2198
Size: 1 10 1 62 1 5233
Fragments arranged by size:
5233 62 10 1 1 1
EcoRI G'AATT_C
Cuts at: 2601 2617 2995 2601
Size: 16 378 4914
Fragments arranged by size:
4914 378 16
FspI TGC'GCA
Cuts at: 3814 3814
Size: 5308
HaeII r_GCGC'y
Cuts at: 1678 2293 2347 4455 1678
Size: 615 54 2108 2531
Fragments arranged by size:
2531 2108 615 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2459 2491 2575 2607 3949
Size: 32 135 32 887 32 84 32 1342
Cuts at: 3949 3981 4023 4055 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1342 887 135 84 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2652 2898 669
Size: 988 281 714 246 3079
Fragments arranged by size:
3079 988 714 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5308
HpaI GTT'AAC
Cuts at: 2898 2898
Size: 5308
KpnI G_GTAC'C
Cuts at: 2633 2633
Size: 5308
MfeI C'AATT_G
Cuts at: 2907 2907
Size: 5308
MluI A'CGCG_T
Cuts at: 5184 5184
Size: 5308
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4422 4606 1305
Size: 3117 184 2007
Fragments arranged by size:
3117 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2245 2316 2430 10
Size: 54 1425 343 413 71 114 2888
Fragments arranged by size:
2888 1425 413 343 114 71 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2405 2524 3266 3625 3784 518
Size: 648 1239 119 742 359 159 2042
Fragments arranged by size:
2042 1239 742 648 359 159 119
MspA1I CmG'CkG
Cuts at: 1606 2724 3401 4549 4794 1606
Size: 1118 677 1148 245 2120
Fragments arranged by size:
2120 1148 1118 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4756
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5308
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5308
NotI GC'GGCC_GC
Cuts at: 2755 2755
Size: 5308
NspI r_CATG'y
Cuts at: 1132 1229 2666 4211 1132
Size: 97 1437 1545 2229
Fragments arranged by size:
2229 1545 1437 97
PasI CC'CwG_GG
Cuts at: 1348 2195 1348
Size: 847 4461
PciI A'CATG_T
Cuts at: 4207 4207
Size: 5308
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5308
PmeI GTTT'AAAC
Cuts at: 2612 2612
Size: 5308
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3377 3409 4890 4922 3377
Size: 32 1481 32 3763
Fragments arranged by size:
3763 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5308
PsiI TTA'TAA
Cuts at: 2878 2878
Size: 5308
PspOMI G'GGCC_C
Cuts at: 2198 2209 2272 2198
Size: 11 63 5234
Fragments arranged by size:
5234 63 11
PstI C_TGCA'G
Cuts at: 830 2660 830
Size: 1830 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5308
SalI G'TCGA_C
Cuts at: 1936 2650 1936
Size: 714 4594
SbfI CC_TGCA'GG
Cuts at: 2660 2660
Size: 5308
ScaI AGT'ACT
Cuts at: 3556 5268 3556
Size: 1712 3596
SfcI C'TryA_G
Cuts at: 826 1045 2656 3791 4472 4663 826
Size: 219 1611 1135 681 191 1471
Fragments arranged by size:
1611 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5308
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5308
SmaI CCC'GGG
Cuts at: 1872 2279 2635 1872
Size: 407 356 4545
Fragments arranged by size:
4545 407 356
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2437 3412 4313 4575 820
Size: 197 926 494 975 901 262 1553
Fragments arranged by size:
1553 975 926 901 494 262 197
SnaBI TAC'GTA
Cuts at: 493 3099 493
Size: 2606 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5308
SphI G_CATG'C
Cuts at: 1132 2666 1132
Size: 1534 3774
SspI AAT'ATT
Cuts at: 5 52 3232 5
Size: 47 3180 2081
Fragments arranged by size:
3180 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5308
StyI C'CwwG_G
Cuts at: 513 1065 2747 513
Size: 552 1682 3074
Fragments arranged by size:
3074 1682 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2284 3335 3352 3505 3690 4135 325
Size: 1255 704 1051 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1051 704 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2229 3554 5266
Size: 274 80 33 51 1443 252 1325 1712
Cuts at: 5266 96
Size: 138
Fragments arranged by size:
1712 1443 1325 274 252 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2121 2153 5268 5300 2121
Size: 32 3115 32 2129
Fragments arranged by size:
3115 2129 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2171 1316
Size: 855 4453
XbaI T'CTAG_A
Cuts at: 2644 2644
Size: 5308
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5308
XmaI C'CCGG_G
Cuts at: 1870 2277 2633 1870
Size: 407 356 4545
Fragments arranged by size:
4545 407 356
XmnI GAAnn'nnTTC
Cuts at: 3437 3437
Size: 5308
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3116 276
Size: 53 83 186 936 1582 2468
Fragments arranged by size:
2468 1582 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI