Restriction map for
pFN21AE5257
(Circular) (Six-base) MAPSORT of: pFN21AE5257.seq Check: 4398 from: 1 to: 5329
pFN21AE5257 5329 bp
With 182 enzymes: SgfI *
January 30, 2014 17:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2058 2120 2058
Size: 62 5267
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3139 278
Size: 53 83 186 936 1603 2468
Fragments arranged by size:
2468 1603 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2672 1937
Size: 735 4594
Acc65I G'GTAC_C
Cuts at: 2650 2650
Size: 5329
AclI AA'CG_TT
Cuts at: 1563 3456 3829 1563
Size: 1893 373 3063
Fragments arranged by size:
3063 1893 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2480 3392 4776 5044 1632
Size: 848 912 1384 268 1917
Fragments arranged by size:
1917 1384 912 848 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5132
AflIII A'CryG_T
Cuts at: 1203 4228 5205 1203
Size: 3025 977 1327
Fragments arranged by size:
3025 1327 977
AgeI A'CCGG_T
Cuts at: 4160 4160
Size: 5329
AhdI GACnn_n'nnGTC
Cuts at: 2137 4058 2137
Size: 1921 3408
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5329
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5329
AlwNI CAG_nnn'CTG
Cuts at: 3072 4644 3072
Size: 1572 3757
ApaLI G'TGCA_C
Cuts at: 3386 4542 3386
Size: 1156 4173
ApoI r'AATT_y
Cuts at: 1493 2438 2638 2864 3016 5125 1493
Size: 945 200 226 152 2109 1697
Fragments arranged by size:
2109 1697 945 226 200 152
AseI AT'TA_AT
Cuts at: 160 1032 3883 160
Size: 872 2851 1606
Fragments arranged by size:
2851 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5329
AvaI C'yCGr_G
Cuts at: 1870 1943 2654 1870
Size: 73 711 4545
Fragments arranged by size:
4545 711 73
BamHI G'GATC_C
Cuts at: 2659 3026 2659
Size: 367 4962
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2650 4105 618
Size: 325 217 627 863 1455 1842
Fragments arranged by size:
1842 1455 863 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2560 2648 1391
Size: 1169 88 4072
Fragments arranged by size:
4072 1169 88
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2088 928
Size: 971 189 4169
Fragments arranged by size:
4169 971 189
BbvCI CC'TCA_GC
Cuts at: 2035 2035
Size: 5329
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3520 3554 1903
Size: 34 1583 34 3678
Fragments arranged by size:
3678 1583 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2010 2477 3221 4431 2010
Size: 467 744 1210 2908
Fragments arranged by size:
2908 1210 744 467
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5329
BfrBI ATG'CAT
Cuts at: 2498 2498
Size: 5329
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3940 136
Size: 107 122 71 631 605 2268 1525
Fragments arranged by size:
2268 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5324 5324
Size: 5329
BlpI GC'TnA_GC
Cuts at: 2746 2746
Size: 5329
Bme1580I G_kGCm'C
Cuts at: 1790 3390 4546 1790
Size: 1600 1156 2573
Fragments arranged by size:
2573 1600 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5329
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3060 4018 442
Size: 416 53 83 399 24 1643 958 1753
Fragments arranged by size:
1753 1643 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5329
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2260 2357 3989 1239
Size: 1021 97 1632 2579
Fragments arranged by size:
2579 1632 1021 97
Bpu10I CC'TnA_GC
Cuts at: 2035 2035
Size: 5329
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2258 3454 4319 4617 2258
Size: 1196 865 298 2970
Fragments arranged by size:
2970 1196 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3992 882
Size: 617 2493 2219
Fragments arranged by size:
2493 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3120 493
Size: 711 1916 2702
Fragments arranged by size:
2702 1916 711
BsaBI GATnn'nnATC
Cuts at: 5323 5323
Size: 5329
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3136 3518 4153 275
Size: 53 83 186 936 1603 382 635 1451
Fragments arranged by size:
1603 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3762 4160 4434 4581 4992 3762
Size: 398 274 147 411 4099
Fragments arranged by size:
4099 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5104
Fragments arranged by size:
5104 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2158 2167 1186
Size: 972 9 4348
Fragments arranged by size:
4348 972 9
BseYI C'CCAG_C
Cuts at: 4532 5028 4532
Size: 496 4833
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4756
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2779 3540 4568 1540
Size: 275 186 778 761 1028 2301
Fragments arranged by size:
2301 1028 778 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2560 3390 3475 4546 2560
Size: 830 85 1071 3343
Fragments arranged by size:
3343 1071 830 85
BsmI GAATG_Cn'
Cuts at: 2840 2933 2840
Size: 93 5236
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2560 2648 3390 3475 4546 1391
Size: 399 770 88 742 85 1071 2174
Fragments arranged by size:
2174 1071 770 742 399 88 85
BspEI T'CCGG_A
Cuts at: 4992 4992
Size: 5329
BspHI T'CATG_A
Cuts at: 3218 4948 3218
Size: 1730 3599
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2058 2120 2684 844
Size: 1214 62 564 3489
Fragments arranged by size:
3489 1214 564 62
BsrBI CCG'CTC
Cuts at: 1394 1823 3216 1394
Size: 429 1393 3507
Fragments arranged by size:
3507 1393 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3824 3998 65
Size: 1183 2576 174 1396
Fragments arranged by size:
2576 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3973 4160 1700
Size: 110 2163 187 2869
Fragments arranged by size:
2869 2163 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5329
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5329
BssSI C'ACGA_G
Cuts at: 3389 4401 3389
Size: 1012 4317
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5329
BstBI TT'CG_AA
Cuts at: 4133 4133
Size: 5329
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5329
BstXI CCAn_nnnn'nTGG
Cuts at: 2123 2123
Size: 5329
BstYI r'GATC_y
Cuts at: 1972 2659 3026 3411 3428 4880 4966 4978 5324
Size: 687 367 385 17 1452 86 12 346
Cuts at: 5324 1972
Size: 1977
Fragments arranged by size:
1977 1452 687 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4777
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4590
Fragments arranged by size:
4590 683 56
BtsI GCAGTG_nn'
Cuts at: 2845 3639 3659 2845
Size: 794 20 4515
Fragments arranged by size:
4515 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5329
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5294
DraI TTT'AAA
Cuts at: 2633 2633
Size: 5329
DraIII CAC_nnn'GTG
Cuts at: 2030 2030
Size: 5329
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4336 809
Size: 507 3020 1802
Fragments arranged by size:
3020 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2776 3665 5294
Size: 54 1425 50 275 18 946 889 1629
Cuts at: 5294 8
Size: 43
Fragments arranged by size:
1629 1425 946 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2776 1537
Size: 275 964 4090
Fragments arranged by size:
4090 964 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2164 3259 1353
Size: 811 1095 3423
Fragments arranged by size:
3423 1095 811
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3916 4290 4436 387
Size: 1064 138 2327 374 146 1280
Fragments arranged by size:
2327 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2558 2558
Size: 5329
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2260 2357 2480 3392 3989 4776 5044
Size: 393 628 97 123 912 597 787 268
Cuts at: 5044 1239
Size: 1524
Fragments arranged by size:
1524 912 787 628 597 393 268 123
97
EcoNI CCTnn'n_nnAGG
Cuts at: 2126 2126
Size: 5329
EcoRI G'AATT_C
Cuts at: 2638 3016 2638
Size: 378 4951
FspI TGC'GCA
Cuts at: 3835 3835
Size: 5329
HaeII r_GCGC'y
Cuts at: 1678 4476 1678
Size: 2798 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2123 2155 2200 2232 2330
Size: 32 135 32 551 32 45 32 98
Cuts at: 2330 2362 3970 4002 4044 4076 1373
Size: 32 1608 32 42 32 2626
Fragments arranged by size:
2626 1608 551 135 98 45 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2673 2919 669
Size: 988 281 735 246 3079
Fragments arranged by size:
3079 988 735 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5329
HpaI GTT'AAC
Cuts at: 2919 2919
Size: 5329
KpnI G_GTAC'C
Cuts at: 2654 2654
Size: 5329
MfeI C'AATT_G
Cuts at: 2928 2928
Size: 5329
MluI A'CGCG_T
Cuts at: 5205 5205
Size: 5329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4443 4627 1305
Size: 3138 184 2007
Fragments arranged by size:
3138 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3507
Fragments arranged by size:
3507 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3287 3646 3805 518
Size: 648 2121 359 159 2042
Fragments arranged by size:
2121 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2238 2301 2745 3422 4570 4815 1606
Size: 632 63 444 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 632 444 245 63
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4777
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5329
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5329
NotI GC'GGCC_GC
Cuts at: 2776 2776
Size: 5329
NsiI A_TGCA'T
Cuts at: 2500 2500
Size: 5329
NspI r_CATG'y
Cuts at: 1132 1229 2204 2687 4232 1132
Size: 97 975 483 1545 2229
Fragments arranged by size:
2229 1545 975 483 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5329
PciI A'CATG_T
Cuts at: 4228 4228
Size: 5329
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5329
PfoI T'CCnGG_A
Cuts at: 2334 2334
Size: 5329
PmeI GTTT'AAAC
Cuts at: 2633 2633
Size: 5329
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3398 3430 4911 4943 3398
Size: 32 1481 32 3784
Fragments arranged by size:
3784 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5329
PsiI TTA'TAA
Cuts at: 2899 2899
Size: 5329
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2309 2341 2309
Size: 32 5297
PstI C_TGCA'G
Cuts at: 830 2681 830
Size: 1851 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5329
SacI G_AGCT'C
Cuts at: 2560 2560
Size: 5329
SalI G'TCGA_C
Cuts at: 1936 2671 1936
Size: 735 4594
SbfI CC_TGCA'GG
Cuts at: 2681 2681
Size: 5329
ScaI AGT'ACT
Cuts at: 2267 3577 5289 2267
Size: 1310 1712 2307
Fragments arranged by size:
2307 1712 1310
SfcI C'TryA_G
Cuts at: 826 1045 2677 3812 4493 4684 826
Size: 219 1632 1135 681 191 1471
Fragments arranged by size:
1632 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5329
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5329
SmaI CCC'GGG
Cuts at: 1872 2656 1872
Size: 784 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2273 3433 4334 4596 820
Size: 197 926 330 1160 901 262 1553
Fragments arranged by size:
1553 1160 926 901 330 262 197
SnaBI TAC'GTA
Cuts at: 493 3120 493
Size: 2627 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5329
SphI G_CATG'C
Cuts at: 1132 2687 1132
Size: 1555 3774
SspI AAT'ATT
Cuts at: 5 52 3253 5
Size: 47 3201 2081
Fragments arranged by size:
3201 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5329
StyI C'CwwG_G
Cuts at: 513 1065 2122 2561 2768 513
Size: 552 1057 439 207 3074
Fragments arranged by size:
3074 1057 552 439 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3356 3373 3526 3711 4156 325
Size: 1255 1776 17 153 185 445 1498
Fragments arranged by size:
1776 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2265 3575 5287
Size: 274 80 33 51 1443 288 1310 1712
Cuts at: 5287 96
Size: 138
Fragments arranged by size:
1712 1443 1310 288 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2187 2219 5289 5321 2187
Size: 32 3070 32 2195
Fragments arranged by size:
3070 2195 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5329
XbaI T'CTAG_A
Cuts at: 2665 2665
Size: 5329
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5329
XmaI C'CCGG_G
Cuts at: 1870 2654 1870
Size: 784 4545
XmnI GAAnn'nnTTC
Cuts at: 3458 3458
Size: 5329
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3137 276
Size: 53 83 186 936 1603 2468
Fragments arranged by size:
2468 1603 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PsrI PstI PvuI SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspOMI PspXI PvuII RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI