Restriction map for
pFN21AE5256
(Circular) (Six-base) MAPSORT of: pFN21AE5256.seq Check: 547 from: 1 to: 5428
pFN21AE5256 5428 bp
With 182 enzymes: SgfI *
January 30, 2014 17:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3238 278
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2771 1937
Size: 834 4594
Acc65I G'GTAC_C
Cuts at: 2749 2749
Size: 5428
AclI AA'CG_TT
Cuts at: 1563 3555 3928 1563
Size: 1992 373 3063
Fragments arranged by size:
3063 1992 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2609 2681 3491 4875 5143 1632
Size: 977 72 810 1384 268 1917
Fragments arranged by size:
1917 1384 977 810 268 72
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5231
AflIII A'CryG_T
Cuts at: 1203 4327 5304 1203
Size: 3124 977 1327
Fragments arranged by size:
3124 1327 977
AgeI A'CCGG_T
Cuts at: 4259 4259
Size: 5428
AhdI GACnn_n'nnGTC
Cuts at: 4157 4157
Size: 5428
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5428
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5428
AlwNI CAG_nnn'CTG
Cuts at: 3171 4743 3171
Size: 1572 3856
ApaLI G'TGCA_C
Cuts at: 3485 4641 3485
Size: 1156 4272
ApoI r'AATT_y
Cuts at: 1493 2396 2411 2737 2963 3115 5224 1493
Size: 903 15 326 226 152 2109 1697
Fragments arranged by size:
2109 1697 903 326 226 152 15
AseI AT'TA_AT
Cuts at: 160 1032 2360 3982 160
Size: 872 1328 1622 1606
Fragments arranged by size:
1622 1606 1328 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
AvaI C'yCGr_G
Cuts at: 1870 1943 2556 2753 1870
Size: 73 613 197 4545
Fragments arranged by size:
4545 613 197 73
BamHI G'GATC_C
Cuts at: 2758 3125 2758
Size: 367 5061
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 2749 4204 618
Size: 325 217 627 351 611 1455 1842
Fragments arranged by size:
1842 1455 627 611 351 325 217
BanII G_rGCy'C
Cuts at: 1391 2073 2344 2747 1391
Size: 682 271 403 4072
Fragments arranged by size:
4072 682 403 271
BbeI G_GCGC'C
Cuts at: 2142 2142
Size: 5428
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2517 928
Size: 971 618 3839
Fragments arranged by size:
3839 971 618
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3619 3653 1903
Size: 34 50 34 1598 34 3678
Fragments arranged by size:
3678 1598 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3320 4530 3320
Size: 1210 4218
BclI T'GATC_A
Cuts at: 1548 2571 1548
Size: 1023 4405
BfrBI ATG'CAT
Cuts at: 2387 2387
Size: 5428
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4039 136
Size: 107 122 71 631 605 2367 1525
Fragments arranged by size:
2367 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2284 5423 2284
Size: 3139 2289
BlpI GC'TnA_GC
Cuts at: 2845 2845
Size: 5428
Bme1580I G_kGCm'C
Cuts at: 1790 3489 4645 1790
Size: 1699 1156 2573
Fragments arranged by size:
2573 1699 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5428
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3159 4117 442
Size: 416 53 83 399 24 1742 958 1753
Fragments arranged by size:
1753 1742 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2355 4088 1239
Size: 818 298 1733 2579
Fragments arranged by size:
2579 1733 818 298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3553 4418 4716 3553
Size: 865 298 4265
Fragments arranged by size:
4265 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4091 882
Size: 617 2592 2219
Fragments arranged by size:
2592 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3219 493
Size: 711 2015 2702
Fragments arranged by size:
2702 2015 711
BsaBI GATnn'nnATC
Cuts at: 5422 5422
Size: 5428
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2139 3235 3617 4252
Size: 53 83 186 936 606 1096 382 635
Cuts at: 4252 275
Size: 1451
Fragments arranged by size:
1451 1096 936 635 606 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3861 4259 4533 4680 5091 3861
Size: 398 274 147 411 4198
Fragments arranged by size:
4198 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5203
Fragments arranged by size:
5203 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5428
BseYI C'CCAG_C
Cuts at: 4631 5127 4631
Size: 496 4932
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4855
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 2052 2878 3639 4667 1540
Size: 275 186 33 18 826 761 1028 2301
Fragments arranged by size:
2301 1028 826 761 275 186 33 18
BsiHKAI G_wGCw'C
Cuts at: 3489 3574 4645 3489
Size: 85 1071 4272
Fragments arranged by size:
4272 1071 85
BsmI GAATG_Cn'
Cuts at: 2387 2389 2939 3032 2387
Size: 2 550 93 4783
Fragments arranged by size:
4783 550 93 2
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2344 2747 3489 3574 4645 1391
Size: 399 283 271 403 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 403 399 283 271 85
BspEI T'CCGG_A
Cuts at: 5091 5091
Size: 5428
BspHI T'CATG_A
Cuts at: 2667 3317 5047 2667
Size: 650 1730 3048
Fragments arranged by size:
3048 1730 650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2783 844
Size: 1939 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2089 2162 3315 1394
Size: 429 266 73 1153 3507
Fragments arranged by size:
3507 1153 429 266 73
BsrDI GCAATG_nn'
Cuts at: 65 1248 3923 4097 65
Size: 1183 2675 174 1396
Fragments arranged by size:
2675 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2029 2051 4072 4259 1700
Size: 110 219 22 2021 187 2869
Fragments arranged by size:
2869 2021 219 187 110 22
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5428
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5428
BssSI C'ACGA_G
Cuts at: 2218 3488 4500 2218
Size: 1270 1012 3146
Fragments arranged by size:
3146 1270 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5428
BstBI TT'CG_AA
Cuts at: 4232 4232
Size: 5428
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5428
BstYI r'GATC_y
Cuts at: 1972 2284 2758 3125 3510 3527 4979 5065 5077
Size: 312 474 367 385 17 1452 86 12
Cuts at: 5077 5423 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 474 385 367 346 312 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2535 2535
Size: 5428
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4876
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4689
Fragments arranged by size:
4689 683 56
BtsI GCAGTG_nn'
Cuts at: 2944 3738 3758 2944
Size: 794 20 4614
Fragments arranged by size:
4614 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5428
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5393
DraI TTT'AAA
Cuts at: 2732 2732
Size: 5428
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4435 809
Size: 507 3119 1802
Fragments arranged by size:
3119 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2049 2875
Size: 54 1425 50 275 18 201 18 826
Cuts at: 2875 3764 5393 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 826 275 201 54 50
43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2031 2049 2875 1537
Size: 275 219 18 826 4090
Fragments arranged by size:
4090 826 275 219 18
EarI CTCTTCn'nnn_
Cuts at: 1353 2438 2481 3358 1353
Size: 1085 43 877 3423
Fragments arranged by size:
3423 1085 877 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2030 4015 4389 4535 387
Size: 1064 138 441 1985 374 146 1280
Fragments arranged by size:
1985 1280 1064 441 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2355 2609 2681 3491 4088 4875
Size: 393 425 298 254 72 810 597 787
Cuts at: 4875 5143 1239
Size: 268 1524
Fragments arranged by size:
1524 810 787 597 425 393 298 268
254 72
EcoRI G'AATT_C
Cuts at: 2396 2411 2737 3115 2396
Size: 15 326 378 4709
Fragments arranged by size:
4709 378 326 15
FspI TGC'GCA
Cuts at: 2009 3934 2009
Size: 1925 3503
FspAI rTGC'GCAy
Cuts at: 2009 2009
Size: 5428
HaeII r_GCGC'y
Cuts at: 1678 2142 4575 1678
Size: 464 2433 2531
Fragments arranged by size:
2531 2433 464
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2556 2588 4069 4101 4143
Size: 32 135 32 984 32 1481 32 42
Cuts at: 4143 4175 1373
Size: 32 2626
Fragments arranged by size:
2626 1481 984 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2332 2772 3018 669
Size: 988 281 394 440 246 3079
Fragments arranged by size:
3079 988 440 394 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5428
HpaI GTT'AAC
Cuts at: 3018 3018
Size: 5428
KasI G'GCGC_C
Cuts at: 2138 2138
Size: 5428
KpnI G_GTAC'C
Cuts at: 2753 2753
Size: 5428
MfeI C'AATT_G
Cuts at: 3027 3027
Size: 5428
MluI A'CGCG_T
Cuts at: 5304 5304
Size: 5428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2202 2315 4542 4726 1305
Size: 897 113 2227 184 2007
Fragments arranged by size:
2227 2007 897 184 113
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3606
Fragments arranged by size:
3606 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2639 3386 3745 3904 518
Size: 648 1473 747 359 159 2042
Fragments arranged by size:
2042 1473 747 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2844 3521 4669 4914 1606
Size: 1238 677 1148 245 2120
Fragments arranged by size:
2120 1238 1148 677 245
NaeI GCC'GGC
Cuts at: 2031 2053 2031
Size: 22 5406
NarI GG'CG_CC
Cuts at: 2139 2139
Size: 5428
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4876
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5428
NgoMIV G'CCGG_C
Cuts at: 2029 2051 2029
Size: 22 5406
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5428
NotI GC'GGCC_GC
Cuts at: 2875 2875
Size: 5428
NsiI A_TGCA'T
Cuts at: 2389 2389
Size: 5428
NspI r_CATG'y
Cuts at: 1132 1229 2187 2786 4331 1132
Size: 97 958 599 1545 2229
Fragments arranged by size:
2229 1545 958 599 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5428
PciI A'CATG_T
Cuts at: 4327 4327
Size: 5428
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5428
PmeI GTTT'AAAC
Cuts at: 2732 2732
Size: 5428
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3497 3529 5010 5042 3497
Size: 32 1481 32 3883
Fragments arranged by size:
3883 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5428
PsiI TTA'TAA
Cuts at: 2998 2998
Size: 5428
PstI C_TGCA'G
Cuts at: 830 2780 830
Size: 1950 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5428
SalI G'TCGA_C
Cuts at: 1936 2770 1936
Size: 834 4594
SapI GCTCTTCn'nnn_
Cuts at: 2438 2438
Size: 5428
SbfI CC_TGCA'GG
Cuts at: 2780 2780
Size: 5428
ScaI AGT'ACT
Cuts at: 3676 5388 3676
Size: 1712 3716
SfcI C'TryA_G
Cuts at: 826 1045 2776 3911 4592 4783 826
Size: 219 1731 1135 681 191 1471
Fragments arranged by size:
1731 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2140 2140
Size: 5428
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5428
SmaI CCC'GGG
Cuts at: 1872 2558 2755 1872
Size: 686 197 4545
Fragments arranged by size:
4545 686 197
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3532 4433 4695 820
Size: 197 926 1589 901 262 1553
Fragments arranged by size:
1589 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3219 493
Size: 2726 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5428
SphI G_CATG'C
Cuts at: 1132 2187 2786 1132
Size: 1055 599 3774
Fragments arranged by size:
3774 1055 599
SspI AAT'ATT
Cuts at: 5 52 3352 5
Size: 47 3300 2081
Fragments arranged by size:
3300 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5428
StyI C'CwwG_G
Cuts at: 513 1065 2867 513
Size: 552 1802 3074
Fragments arranged by size:
3074 1802 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3455 3472 3625 3810 4255 325
Size: 1255 1875 17 153 185 445 1498
Fragments arranged by size:
1875 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3674 5386 96
Size: 274 80 33 51 1443 1697 1712 138
Fragments arranged by size:
1712 1697 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2431 2463 5388 5420 2431
Size: 32 2925 32 2439
Fragments arranged by size:
2925 2439 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5428
XbaI T'CTAG_A
Cuts at: 2764 2764
Size: 5428
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5428
XmaI C'CCGG_G
Cuts at: 1870 2556 2753 1870
Size: 686 197 4545
Fragments arranged by size:
4545 686 197
XmnI GAAnn'nnTTC
Cuts at: 2387 3557 2387
Size: 1170 4258
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3236 276
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SrfI SwaI XcmI