Restriction map for
pFN21AE5255
(Circular) (Six-base) MAPSORT of: pFN21AE5255.seq Check: 2475 from: 1 to: 5224
pFN21AE5255 5224 bp
With 182 enzymes: SgfI *
December 8, 2014 13:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3034 278
Size: 53 83 186 936 1498 2468
Fragments arranged by size:
2468 1498 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2567 1937
Size: 630 4594
Acc65I G'GTAC_C
Cuts at: 2545 2545
Size: 5224
AclI AA'CG_TT
Cuts at: 1563 3351 3724 1563
Size: 1788 373 3063
Fragments arranged by size:
3063 1788 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3287 4671 4939 1632
Size: 1655 1384 268 1917
Fragments arranged by size:
1917 1655 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5027
AflIII A'CryG_T
Cuts at: 1203 4123 5100 1203
Size: 2920 977 1327
Fragments arranged by size:
2920 1327 977
AgeI A'CCGG_T
Cuts at: 4055 4055
Size: 5224
AhdI GACnn_n'nnGTC
Cuts at: 3953 3953
Size: 5224
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5224
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5224
AlwNI CAG_nnn'CTG
Cuts at: 2068 2967 4539 2068
Size: 899 1572 2753
Fragments arranged by size:
2753 1572 899
ApaLI G'TGCA_C
Cuts at: 2317 2507 3281 4437 2317
Size: 190 774 1156 3104
Fragments arranged by size:
3104 1156 774 190
ApoI r'AATT_y
Cuts at: 1493 2533 2759 2911 5020 1493
Size: 1040 226 152 2109 1697
Fragments arranged by size:
2109 1697 1040 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3778 160
Size: 872 2746 1606
Fragments arranged by size:
2746 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5224
AvaI C'yCGr_G
Cuts at: 1870 1943 2549 1870
Size: 73 606 4545
Fragments arranged by size:
4545 606 73
BamHI G'GATC_C
Cuts at: 2554 2921 2554
Size: 367 4857
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2146 2545 4000 618
Size: 325 217 627 359 399 1455 1842
Fragments arranged by size:
1842 1455 627 399 359 325 217
BanII G_rGCy'C
Cuts at: 1391 2051 2543 1391
Size: 660 492 4072
Fragments arranged by size:
4072 660 492
BbeI G_GCGC'C
Cuts at: 2150 2150
Size: 5224
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2286 928
Size: 971 387 3866
Fragments arranged by size:
3866 971 387
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3415 3449 1903
Size: 34 1478 34 3678
Fragments arranged by size:
3678 1478 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3116 4326 3116
Size: 1210 4014
BclI T'GATC_A
Cuts at: 1548 2124 1548
Size: 576 4648
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3835 136
Size: 107 122 71 631 605 2163 1525
Fragments arranged by size:
2163 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5219 5219
Size: 5224
BlpI GC'TnA_GC
Cuts at: 2409 2641 2409
Size: 232 4992
Bme1580I G_kGCm'C
Cuts at: 1790 2321 2511 3285 4441 1790
Size: 531 190 774 1156 2573
Fragments arranged by size:
2573 1156 774 531 190
BmgBI CAC'GTC
Cuts at: 1318 2207 1318
Size: 889 4335
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2955 3913 442
Size: 416 53 83 399 24 1538 958 1753
Fragments arranged by size:
1753 1538 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2397 3884 1239
Size: 1158 1487 2579
Fragments arranged by size:
2579 1487 1158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3349 4214 4512 3349
Size: 865 298 4061
Fragments arranged by size:
4061 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3887 882
Size: 617 2388 2219
Fragments arranged by size:
2388 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3015 493
Size: 711 1811 2702
Fragments arranged by size:
2702 1811 711
BsaBI GATnn'nnATC
Cuts at: 5218 5218
Size: 5224
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2147 3031 3413 4048
Size: 53 83 186 936 614 884 382 635
Cuts at: 4048 275
Size: 1451
Fragments arranged by size:
1451 936 884 635 614 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3657 4055 4329 4476 4887 3657
Size: 398 274 147 411 3994
Fragments arranged by size:
3994 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2135 2165 1178
Size: 30 165 30 732 30 4237
Fragments arranged by size:
4237 732 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5224
BseYI C'CCAG_C
Cuts at: 2218 2397 4427 4923 2218
Size: 179 2030 496 2519
Fragments arranged by size:
2519 2030 496 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2037 1163
Size: 573 301 4350
Fragments arranged by size:
4350 573 301
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2674 3435 4463 1540
Size: 275 186 673 761 1028 2301
Fragments arranged by size:
2301 1028 761 673 275 186
BsiHKAI G_wGCw'C
Cuts at: 2321 2511 3285 3370 4441 2321
Size: 190 774 85 1071 3104
Fragments arranged by size:
3104 1071 774 190 85
BsmI GAATG_Cn'
Cuts at: 2735 2828 2735
Size: 93 5131
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2321 2511 2543 3285 3370 4441
Size: 399 261 270 190 32 742 85 1071
Cuts at: 4441 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 270 261 190 85
32
BspEI T'CCGG_A
Cuts at: 4887 4887
Size: 5224
BspHI T'CATG_A
Cuts at: 3113 4843 3113
Size: 1730 3494
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2009 2579 844
Size: 1165 570 3489
Fragments arranged by size:
3489 1165 570
BsrBI CCG'CTC
Cuts at: 1394 1823 2169 3111 1394
Size: 429 346 942 3507
Fragments arranged by size:
3507 942 429 346
BsrDI GCAATG_nn'
Cuts at: 65 1248 3719 3893 65
Size: 1183 2471 174 1396
Fragments arranged by size:
2471 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3868 4055 1700
Size: 110 2058 187 2869
Fragments arranged by size:
2869 2058 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5224
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5224
BssSI C'ACGA_G
Cuts at: 3284 4296 3284
Size: 1012 4212
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5224
BstBI TT'CG_AA
Cuts at: 4028 4028
Size: 5224
BstEII G'GTnAC_C
Cuts at: 1184 2021 2033 1184
Size: 837 12 4375
Fragments arranged by size:
4375 837 12
BstYI r'GATC_y
Cuts at: 1972 2554 2921 3306 3323 4775 4861 4873 5219
Size: 582 367 385 17 1452 86 12 346
Cuts at: 5219 1972
Size: 1977
Fragments arranged by size:
1977 1452 582 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2350 513
Size: 552 939 346 3387
Fragments arranged by size:
3387 939 552 346
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4485
Fragments arranged by size:
4485 683 56
BtsI GCAGTG_nn'
Cuts at: 2091 2511 2740 3534 3554 2091
Size: 420 229 794 20 3761
Fragments arranged by size:
3761 794 420 229 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5224
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5189
DraI TTT'AAA
Cuts at: 2528 2528
Size: 5224
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4231 809
Size: 507 2915 1802
Fragments arranged by size:
2915 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2671 3560 5189
Size: 54 1425 50 275 18 841 889 1629
Cuts at: 5189 8
Size: 43
Fragments arranged by size:
1629 1425 889 841 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2671 1537
Size: 275 859 4090
Fragments arranged by size:
4090 859 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3154 1353
Size: 1801 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3811 4185 4331 387
Size: 1064 138 2222 374 146 1280
Fragments arranged by size:
2222 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2397 3287 3884 4671 4939 1239
Size: 393 765 890 597 787 268 1524
Fragments arranged by size:
1524 890 787 765 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2060 2060
Size: 5224
EcoO109I rG'GnC_Cy
Cuts at: 2355 2355
Size: 5224
EcoRI G'AATT_C
Cuts at: 2533 2911 2533
Size: 378 4846
EcoRV GAT'ATC
Cuts at: 2385 2385
Size: 5224
FspI TGC'GCA
Cuts at: 3730 3730
Size: 5224
HaeII r_GCGC'y
Cuts at: 1678 2150 4371 1678
Size: 472 2221 2531
Fragments arranged by size:
2531 2221 472
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3865 3897 3939 3971 1373
Size: 32 135 32 2293 32 42 32 2626
Fragments arranged by size:
2626 2293 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2568 2814 669
Size: 988 281 630 246 3079
Fragments arranged by size:
3079 988 630 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5224
HpaI GTT'AAC
Cuts at: 2814 2814
Size: 5224
KasI G'GCGC_C
Cuts at: 2146 2146
Size: 5224
KpnI G_GTAC'C
Cuts at: 2549 2549
Size: 5224
MfeI C'AATT_G
Cuts at: 2823 2823
Size: 5224
MluI A'CGCG_T
Cuts at: 5100 5100
Size: 5224
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2287 4338 4522 1305
Size: 982 2051 184 2007
Fragments arranged by size:
2051 2007 982 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3402
Fragments arranged by size:
3402 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2498 3182 3541 3700 518
Size: 648 1332 684 359 159 2042
Fragments arranged by size:
2042 1332 684 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2218 2352 2640 3317 4465 4710 1606
Size: 612 134 288 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 612 288 245 134
NarI GG'CG_CC
Cuts at: 2147 2147
Size: 5224
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3733
Fragments arranged by size:
3733 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5224
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5224
NotI GC'GGCC_GC
Cuts at: 2671 2671
Size: 5224
NspI r_CATG'y
Cuts at: 1132 1229 2375 2582 4127 1132
Size: 97 1146 207 1545 2229
Fragments arranged by size:
2229 1545 1146 207 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5224
PciI A'CATG_T
Cuts at: 4123 4123
Size: 5224
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5224
PmeI GTTT'AAAC
Cuts at: 2528 2528
Size: 5224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3293 3325 4806 4838 3293
Size: 32 1481 32 3679
Fragments arranged by size:
3679 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2355 2355
Size: 5224
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5224
PsiI TTA'TAA
Cuts at: 2794 2794
Size: 5224
PstI C_TGCA'G
Cuts at: 830 2576 830
Size: 1746 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5224
SacII CC_GC'GG
Cuts at: 2353 2353
Size: 5224
SalI G'TCGA_C
Cuts at: 1936 2566 1936
Size: 630 4594
SanDI GG'GwC_CC
Cuts at: 2355 2355
Size: 5224
SbfI CC_TGCA'GG
Cuts at: 2576 2576
Size: 5224
ScaI AGT'ACT
Cuts at: 3472 5184 3472
Size: 1712 3512
SfcI C'TryA_G
Cuts at: 826 1045 2572 3707 4388 4579 826
Size: 219 1527 1135 681 191 1471
Fragments arranged by size:
1527 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2148 2148
Size: 5224
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5224
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5224
SmaI CCC'GGG
Cuts at: 1872 2551 1872
Size: 679 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3328 4229 4491 820
Size: 197 926 1385 901 262 1553
Fragments arranged by size:
1553 1385 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3015 493
Size: 2522 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5224
SphI G_CATG'C
Cuts at: 1132 2375 2582 1132
Size: 1243 207 3774
Fragments arranged by size:
3774 1243 207
SspI AAT'ATT
Cuts at: 5 52 3148 5
Size: 47 3096 2081
Fragments arranged by size:
3096 2081 47
StuI AGG'CCT
Cuts at: 1517 2189 1517
Size: 672 4552
StyI C'CwwG_G
Cuts at: 513 1065 2004 2262 2663 513
Size: 552 939 258 401 3074
Fragments arranged by size:
3074 939 552 401 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2234 3251 3268 3421 3606 4051 325
Size: 1255 654 1017 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1017 654 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3470 5182 96
Size: 274 80 33 51 1443 1493 1712 138
Fragments arranged by size:
1712 1493 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2070 2102 2196 2228 2419 2451 5184 5216 2070
Size: 32 94 32 191 32 2733 32 2078
Fragments arranged by size:
2733 2078 191 94 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2359 1316
Size: 1043 4181
XbaI T'CTAG_A
Cuts at: 2449 2560 2449
Size: 111 5113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2486 2486
Size: 5224
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5224
XmaI C'CCGG_G
Cuts at: 1870 2549 1870
Size: 679 4545
XmnI GAAnn'nnTTC
Cuts at: 3353 3353
Size: 5224
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3032 276
Size: 53 83 186 936 1498 2468
Fragments arranged by size:
2468 1498 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SacII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SapI SexAI SfiI SrfI SwaI