Restriction map for
pFN21AE5254
(Circular) (Six-base) MAPSORT of: pFN21AE5254.seq Check: 5769 from: 1 to: 5341
pFN21AE5254 5341 bp
With 182 enzymes: SgfI *
January 30, 2014 17:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3151 278
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2684 1937
Size: 747 4594
Acc65I G'GTAC_C
Cuts at: 2662 2662
Size: 5341
AclI AA'CG_TT
Cuts at: 1563 3468 3841 1563
Size: 1905 373 3063
Fragments arranged by size:
3063 1905 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3404 4788 5056 1632
Size: 1772 1384 268 1917
Fragments arranged by size:
1917 1772 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2093 820
Size: 197 1076 4068
Fragments arranged by size:
4068 1076 197
AflIII A'CryG_T
Cuts at: 1203 2385 2401 4240 5217 1203
Size: 1182 16 1839 977 1327
Fragments arranged by size:
1839 1327 1182 977 16
AgeI A'CCGG_T
Cuts at: 4172 4172
Size: 5341
AhdI GACnn_n'nnGTC
Cuts at: 4070 4070
Size: 5341
AleI CACnn'nnGTG
Cuts at: 1166 2486 1166
Size: 1320 4021
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5341
AlwNI CAG_nnn'CTG
Cuts at: 3084 4656 3084
Size: 1572 3769
ApaI G_GGCC'C
Cuts at: 2140 2140
Size: 5341
ApaLI G'TGCA_C
Cuts at: 3398 4554 3398
Size: 1156 4185
ApoI r'AATT_y
Cuts at: 1493 2650 2876 3028 5137 1493
Size: 1157 226 152 2109 1697
Fragments arranged by size:
2109 1697 1157 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3895 160
Size: 872 2863 1606
Fragments arranged by size:
2863 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
AvaI C'yCGr_G
Cuts at: 1870 1943 2333 2666 1870
Size: 73 390 333 4545
Fragments arranged by size:
4545 390 333 73
BamHI G'GATC_C
Cuts at: 2671 3038 2671
Size: 367 4974
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2662 4117 618
Size: 325 217 627 875 1455 1842
Fragments arranged by size:
1842 1455 875 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2032 2140 2660 1391
Size: 641 108 520 4072
Fragments arranged by size:
4072 641 520 108
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3532 3566 1903
Size: 34 1595 34 3678
Fragments arranged by size:
3678 1595 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2351 3233 4443 2351
Size: 882 1210 3249
Fragments arranged by size:
3249 1210 882
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5341
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3952 136
Size: 107 122 71 631 605 2280 1525
Fragments arranged by size:
2280 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5336 5336
Size: 5341
BlpI GC'TnA_GC
Cuts at: 2758 2758
Size: 5341
Bme1580I G_kGCm'C
Cuts at: 1790 2140 2275 3402 4558 1790
Size: 350 135 1127 1156 2573
Fragments arranged by size:
2573 1156 1127 350 135
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5341
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3072 4030 442
Size: 416 53 83 399 24 1655 958 1753
Fragments arranged by size:
1753 1655 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 4001 1239
Size: 929 1833 2579
Fragments arranged by size:
2579 1833 929
Bpu10I CC'TnA_GC
Cuts at: 2024 2417 2024
Size: 393 4948
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3466 4331 4629 3466
Size: 865 298 4178
Fragments arranged by size:
4178 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2270 4004 882
Size: 617 771 1734 2219
Fragments arranged by size:
2219 1734 771 617
BsaAI yAC'GTr
Cuts at: 493 1204 3132 493
Size: 711 1928 2702
Fragments arranged by size:
2702 1928 711
BsaBI GATnn'nnATC
Cuts at: 5335 5335
Size: 5341
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3148 3530 4165 275
Size: 53 83 186 936 1615 382 635 1451
Fragments arranged by size:
1615 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2103 2169 3774 4172 4446 4593 5004 2103
Size: 66 1605 398 274 147 411 2440
Fragments arranged by size:
2440 1605 411 398 274 147 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2180 2210 1178
Size: 30 165 30 777 30 4309
Fragments arranged by size:
4309 777 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5341
BseYI C'CCAG_C
Cuts at: 2305 4544 5040 2305
Size: 2239 496 2606
Fragments arranged by size:
2606 2239 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4768
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2791 3552 4580 1540
Size: 275 186 790 761 1028 2301
Fragments arranged by size:
2301 1028 790 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3402 3487 4558 3402
Size: 85 1071 4185
Fragments arranged by size:
4185 1071 85
BsmI GAATG_Cn'
Cuts at: 2315 2852 2945 2315
Size: 537 93 4711
Fragments arranged by size:
4711 537 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2140 2275 2660 3402 3487 4558
Size: 399 242 108 135 385 742 85 1071
Cuts at: 4558 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 385 242 135 108
85
BspEI T'CCGG_A
Cuts at: 2103 2169 5004 2103
Size: 66 2835 2440
Fragments arranged by size:
2835 2440 66
BspHI T'CATG_A
Cuts at: 2154 3230 4960 2154
Size: 1076 1730 2535
Fragments arranged by size:
2535 1730 1076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2696 844
Size: 1852 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3228 1394
Size: 429 1405 3507
Fragments arranged by size:
3507 1405 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2590 3836 4010 65
Size: 1183 1342 1246 174 1396
Fragments arranged by size:
1396 1342 1246 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3985 4172 1700
Size: 110 2175 187 2869
Fragments arranged by size:
2869 2175 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5341
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5341
BssSI C'ACGA_G
Cuts at: 3401 4413 3401
Size: 1012 4329
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5341
BstBI TT'CG_AA
Cuts at: 4145 4145
Size: 5341
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5341
BstYI r'GATC_y
Cuts at: 1972 2671 3038 3423 3440 4892 4978 4990 5336
Size: 699 367 385 17 1452 86 12 346
Cuts at: 5336 1972
Size: 1977
Fragments arranged by size:
1977 1452 699 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3850
Fragments arranged by size:
3850 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4602
Fragments arranged by size:
4602 683 56
BtsI GCAGTG_nn'
Cuts at: 2051 2481 2857 3651 3671 2051
Size: 430 376 794 20 3721
Fragments arranged by size:
3721 794 430 376 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5341
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5306
DraI TTT'AAA
Cuts at: 2645 2645
Size: 5341
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4348 809
Size: 507 3032 1802
Fragments arranged by size:
3032 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2085 2788 3677
Size: 54 1425 50 275 18 255 703 889
Cuts at: 3677 5306 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 703 275 255 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2788 1537
Size: 275 976 4090
Fragments arranged by size:
4090 976 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2171 3271 1353
Size: 818 1100 3423
Fragments arranged by size:
3423 1100 818
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3928 4302 4448 387
Size: 1064 138 2339 374 146 1280
Fragments arranged by size:
2339 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 3404 4001 4788 5056 1239
Size: 393 536 1236 597 787 268 1524
Fragments arranged by size:
1524 1236 787 597 536 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2573 2573
Size: 5341
EcoO109I rG'GnC_Cy
Cuts at: 2137 2464 2137
Size: 327 5014
EcoRI G'AATT_C
Cuts at: 2650 3028 2650
Size: 378 4963
FspI TGC'GCA
Cuts at: 2060 3847 2060
Size: 1787 3554
HaeII r_GCGC'y
Cuts at: 1678 2068 4488 1678
Size: 390 2420 2531
Fragments arranged by size:
2531 2420 390
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2115 2147 3982 4014 4056
Size: 32 135 32 543 32 1835 32 42
Cuts at: 4056 4088 1373
Size: 32 2626
Fragments arranged by size:
2626 1835 543 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2047 2685 2931 669
Size: 988 281 109 638 246 3079
Fragments arranged by size:
3079 988 638 281 246 109
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5341
HpaI GTT'AAC
Cuts at: 2931 2931
Size: 5341
KpnI G_GTAC'C
Cuts at: 2666 2666
Size: 5341
MfeI C'AATT_G
Cuts at: 2940 2940
Size: 5341
MluI A'CGCG_T
Cuts at: 2401 5217 2401
Size: 2816 2525
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4455 4639 1305
Size: 3150 184 2007
Fragments arranged by size:
3150 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3519
Fragments arranged by size:
3519 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2486 3299 3658 3817 518
Size: 648 1320 813 359 159 2042
Fragments arranged by size:
2042 1320 813 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2476 2757 3434 4582 4827 1606
Size: 870 281 677 1148 245 2120
Fragments arranged by size:
2120 1148 870 677 281 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3850
Fragments arranged by size:
3850 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5341
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5341
NotI GC'GGCC_GC
Cuts at: 2788 2788
Size: 5341
NspI r_CATG'y
Cuts at: 1132 1229 2389 2699 4244 1132
Size: 97 1160 310 1545 2229
Fragments arranged by size:
2229 1545 1160 310 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5341
PciI A'CATG_T
Cuts at: 2385 4240 2385
Size: 1855 3486
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5341
PfoI T'CCnGG_A
Cuts at: 2184 2228 2184
Size: 44 5297
PmeI GTTT'AAAC
Cuts at: 2645 2645
Size: 5341
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 4923 4955 3410
Size: 32 1481 32 3796
Fragments arranged by size:
3796 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2464 2464
Size: 5341
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5341
PsiI TTA'TAA
Cuts at: 2911 2911
Size: 5341
PspOMI G'GGCC_C
Cuts at: 2136 2136
Size: 5341
PstI C_TGCA'G
Cuts at: 830 2211 2280 2541 2553 2693 830
Size: 1381 69 261 12 140 3478
Fragments arranged by size:
3478 1381 261 140 69 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5341
SalI G'TCGA_C
Cuts at: 1936 2683 1936
Size: 747 4594
SanDI GG'GwC_CC
Cuts at: 2464 2464
Size: 5341
SbfI CC_TGCA'GG
Cuts at: 2693 2693
Size: 5341
ScaI AGT'ACT
Cuts at: 3589 5301 3589
Size: 1712 3629
SfcI C'TryA_G
Cuts at: 826 1045 2207 2276 2430 2537 2549 2689 3824
Size: 219 1162 69 154 107 12 140 1135
Cuts at: 3824 4505 4696 826
Size: 681 191 1471
Fragments arranged by size:
1471 1162 1135 681 219 191 154 140
107 69 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5341
SmaI CCC'GGG
Cuts at: 1872 2668 1872
Size: 796 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2093 3445 4346 4608 820
Size: 197 926 150 1352 901 262 1553
Fragments arranged by size:
1553 1352 926 901 262 197 150
SnaBI TAC'GTA
Cuts at: 493 3132 493
Size: 2639 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5341
SphI G_CATG'C
Cuts at: 1132 2699 1132
Size: 1567 3774
SspI AAT'ATT
Cuts at: 5 52 3265 5
Size: 47 3213 2081
Fragments arranged by size:
3213 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5341
StyI C'CwwG_G
Cuts at: 513 1065 2004 2780 513
Size: 552 939 776 3074
Fragments arranged by size:
3074 939 776 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3368 3385 3538 3723 4168 325
Size: 1255 1788 17 153 185 445 1498
Fragments arranged by size:
1788 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3587 5299 96
Size: 274 80 33 51 1443 1610 1712 138
Fragments arranged by size:
1712 1610 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2180 2212 2301 2333 5301 5333 2180
Size: 32 89 32 2968 32 2188
Fragments arranged by size:
2968 2188 89 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5341
XbaI T'CTAG_A
Cuts at: 2677 2677
Size: 5341
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5341
XmaI C'CCGG_G
Cuts at: 1870 2666 1870
Size: 796 4545
XmnI GAAnn'nnTTC
Cuts at: 3470 3470
Size: 5341
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3149 276
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI XcmI