Restriction map for
pFN21AE5244
(Circular) (Six-base) MAPSORT of: pFN21AE5244.seq Check: 5392 from: 1 to: 5413
pFN21AE5244 5413 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3223 278
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2553 2756 1937
Size: 616 203 4594
Fragments arranged by size:
4594 616 203
Acc65I G'GTAC_C
Cuts at: 2734 2734
Size: 5413
AclI AA'CG_TT
Cuts at: 1563 3540 3913 1563
Size: 1977 373 3063
Fragments arranged by size:
3063 1977 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2183 3476 4860 5128 1632
Size: 551 1293 1384 268 1917
Fragments arranged by size:
1917 1384 1293 551 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5216
AflIII A'CryG_T
Cuts at: 1203 2655 2657 4312 5289 1203
Size: 1452 2 1655 977 1327
Fragments arranged by size:
1655 1452 1327 977 2
AgeI A'CCGG_T
Cuts at: 4244 4244
Size: 5413
AhdI GACnn_n'nnGTC
Cuts at: 4142 4142
Size: 5413
AleI CACnn'nnGTG
Cuts at: 1166 2132 1166
Size: 966 4447
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2549 1184
Size: 1365 4048
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2630 2662 2630
Size: 32 5381
AlwNI CAG_nnn'CTG
Cuts at: 2395 2398 3156 4728 2395
Size: 3 758 1572 3080
Fragments arranged by size:
3080 1572 758 3
ApaLI G'TGCA_C
Cuts at: 2702 3470 4626 2702
Size: 768 1156 3489
Fragments arranged by size:
3489 1156 768
ApoI r'AATT_y
Cuts at: 1493 2722 2948 3100 5209 1493
Size: 1229 226 152 2109 1697
Fragments arranged by size:
2109 1697 1229 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3967 160
Size: 872 2935 1606
Fragments arranged by size:
2935 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
AvaI C'yCGr_G
Cuts at: 1870 1943 2738 1870
Size: 73 795 4545
Fragments arranged by size:
4545 795 73
BamHI G'GATC_C
Cuts at: 2743 3110 2743
Size: 367 5046
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2287 2362 2581 2734 4189
Size: 325 217 627 500 75 219 153 1455
Cuts at: 4189 618
Size: 1842
Fragments arranged by size:
1842 1455 627 500 325 219 217 153
75
BanII G_rGCy'C
Cuts at: 1391 2732 1391
Size: 1341 4072
BbeI G_GCGC'C
Cuts at: 2291 2585 2291
Size: 294 5119
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4442
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3604 3638 1903
Size: 34 1667 34 3678
Fragments arranged by size:
3678 1667 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3305 4515 3305
Size: 1210 4203
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5413
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4024 136
Size: 107 122 71 631 605 2352 1525
Fragments arranged by size:
2352 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5408 5408
Size: 5413
BlpI GC'TnA_GC
Cuts at: 2830 2830
Size: 5413
Bme1580I G_kGCm'C
Cuts at: 1790 2367 2706 3474 4630 1790
Size: 577 339 768 1156 2573
Fragments arranged by size:
2573 1156 768 577 339
BmgBI CAC'GTC
Cuts at: 1318 2158 1318
Size: 840 4573
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3144 4102 442
Size: 416 53 83 399 24 1727 958 1753
Fragments arranged by size:
1753 1727 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2006 2270 2462 4073 1239
Size: 767 264 192 1611 2579
Fragments arranged by size:
2579 1611 767 264 192
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2090 3538 4403 4701 2090
Size: 1448 865 298 2802
Fragments arranged by size:
2802 1448 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2006 4076 882
Size: 617 507 2070 2219
Fragments arranged by size:
2219 2070 617 507
BsaAI yAC'GTr
Cuts at: 493 1204 2658 3204 493
Size: 711 1454 546 2702
Fragments arranged by size:
2702 1454 711 546
BsaBI GATnn'nnATC
Cuts at: 5407 5407
Size: 5413
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2214 2288 2582 3220
Size: 53 83 186 936 681 74 294 638
Cuts at: 3220 3602 4237 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 681 638 635 382 294 186
83 74 53
BsaWI w'CCGG_w
Cuts at: 3846 4244 4518 4665 5076 3846
Size: 398 274 147 411 4183
Fragments arranged by size:
4183 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2057 2087 2324 2354 2630
Size: 30 165 30 654 30 237 30 276
Cuts at: 2630 2660 1178
Size: 30 3931
Fragments arranged by size:
3931 654 276 237 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2267 1186
Size: 907 174 4332
Fragments arranged by size:
4332 907 174
BseYI C'CCAG_C
Cuts at: 2260 2279 4616 5112 2260
Size: 19 2337 496 2561
Fragments arranged by size:
2561 2337 496 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2528 1163
Size: 573 792 4048
Fragments arranged by size:
4048 792 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2863 3624 4652 1540
Size: 275 186 862 761 1028 2301
Fragments arranged by size:
2301 1028 862 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2448 2706 3474 3559 4630 2448
Size: 258 768 85 1071 3231
Fragments arranged by size:
3231 1071 768 258 85
BsiWI C'GTAC_G
Cuts at: 2608 2608
Size: 5413
BsmI GAATG_Cn'
Cuts at: 2924 3017 2924
Size: 93 5320
BsmBI CGTCTCn'nnnn_
Cuts at: 2249 2249
Size: 5413
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2367 2448 2706 2732 3474 3559 4630
Size: 399 577 81 258 26 742 85 1071
Cuts at: 4630 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 577 399 258 85 81
26
BspEI T'CCGG_A
Cuts at: 5076 5076
Size: 5413
BspHI T'CATG_A
Cuts at: 3302 5032 3302
Size: 1730 3683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2136 2436 2768 844
Size: 1292 300 332 3489
Fragments arranged by size:
3489 1292 332 300
BsrBI CCG'CTC
Cuts at: 1394 1823 3300 1394
Size: 429 1477 3507
Fragments arranged by size:
3507 1477 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3908 4082 65
Size: 1183 2660 174 1396
Fragments arranged by size:
2660 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2315 2691 4057 4244 1700
Size: 110 505 376 1366 187 2869
Fragments arranged by size:
2869 1366 505 376 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5413
BssHII G'CGCG_C
Cuts at: 1925 2401 2431 2433 2565 1925
Size: 476 30 2 132 4773
Fragments arranged by size:
4773 476 132 30 2
BssSI C'ACGA_G
Cuts at: 2447 3473 4485 2447
Size: 1026 1012 3375
Fragments arranged by size:
3375 1026 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2398 794
Size: 1604 3809
BstBI TT'CG_AA
Cuts at: 4217 4217
Size: 5413
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5413
BstYI r'GATC_y
Cuts at: 1972 2676 2743 3110 3495 3512 4964 5050 5062
Size: 704 67 367 385 17 1452 86 12
Cuts at: 5062 5408 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 704 385 367 346 86 67
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2130 2592 513
Size: 552 939 126 462 3334
Fragments arranged by size:
3334 939 552 462 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2645 487
Size: 683 56 1419 3255
Fragments arranged by size:
3255 1419 683 56
BtsI GCAGTG_nn'
Cuts at: 2065 2929 3723 3743 2065
Size: 864 794 20 3735
Fragments arranged by size:
3735 864 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5413
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5378
DraI TTT'AAA
Cuts at: 2717 2717
Size: 5413
DraIII CAC_nnn'GTG
Cuts at: 2562 2562
Size: 5413
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4420 809
Size: 507 3104 1802
Fragments arranged by size:
3104 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2517 2860 3749
Size: 54 1425 50 275 18 687 343 889
Cuts at: 3749 5378 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 687 343 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2860 1537
Size: 275 1048 4090
Fragments arranged by size:
4090 1048 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2093 3343 1353
Size: 740 1250 3423
Fragments arranged by size:
3423 1250 740
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2564 4000 4374 4520 387
Size: 1064 138 975 1436 374 146 1280
Fragments arranged by size:
1436 1280 1064 975 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2006 2183 2270 2462 3476 4073 4860
Size: 393 374 177 87 192 1014 597 787
Cuts at: 4860 5128 1239
Size: 268 1524
Fragments arranged by size:
1524 1014 787 597 393 374 268 192
177 87
EcoO109I rG'GnC_Cy
Cuts at: 2148 2148
Size: 5413
EcoRI G'AATT_C
Cuts at: 2722 3100 2722
Size: 378 5035
FspI TGC'GCA
Cuts at: 3919 3919
Size: 5413
HaeII r_GCGC'y
Cuts at: 1678 2291 2585 2673 4560 1678
Size: 613 294 88 1887 2531
Fragments arranged by size:
2531 1887 613 294 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2047 2079 4054 4086 4128
Size: 32 135 32 475 32 1975 32 42
Cuts at: 4128 4160 1373
Size: 32 2626
Fragments arranged by size:
2626 1975 475 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2554 2757 3003 669
Size: 988 281 616 203 246 3079
Fragments arranged by size:
3079 988 616 281 246 203
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5413
HpaI GTT'AAC
Cuts at: 3003 3003
Size: 5413
KasI G'GCGC_C
Cuts at: 2287 2581 2287
Size: 294 5119
KpnI G_GTAC'C
Cuts at: 2738 2738
Size: 5413
MfeI C'AATT_G
Cuts at: 3012 3012
Size: 5413
MluI A'CGCG_T
Cuts at: 5289 5289
Size: 5413
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2331 4527 4711 1305
Size: 1026 2196 184 2007
Fragments arranged by size:
2196 2007 1026 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3591
Fragments arranged by size:
3591 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2132 3371 3730 3889 518
Size: 648 966 1239 359 159 2042
Fragments arranged by size:
2042 1239 966 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 2083 2188 2395 2470 2594 2829 3506
Size: 420 57 105 207 75 124 235 677
Cuts at: 3506 4654 4899 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 420 245 235 207 124
105 75 57
NaeI GCC'GGC
Cuts at: 2317 2317
Size: 5413
NarI GG'CG_CC
Cuts at: 2288 2582 2288
Size: 294 5119
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3922
Fragments arranged by size:
3922 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5413
NgoMIV G'CCGG_C
Cuts at: 2315 2315
Size: 5413
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5413
NotI GC'GGCC_GC
Cuts at: 2860 2860
Size: 5413
NspI r_CATG'y
Cuts at: 1132 1229 2771 4316 1132
Size: 97 1542 1545 2229
Fragments arranged by size:
2229 1545 1542 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5413
PciI A'CATG_T
Cuts at: 4312 4312
Size: 5413
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5413
PfoI T'CCnGG_A
Cuts at: 2493 2493
Size: 5413
PmeI GTTT'AAAC
Cuts at: 2717 2717
Size: 5413
PmlI CAC'GTG
Cuts at: 2658 2658
Size: 5413
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2630 2662 3482 3514 4995 5027 2630
Size: 32 820 32 1481 32 3016
Fragments arranged by size:
3016 1481 820 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2148 2148
Size: 5413
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5413
PsiI TTA'TAA
Cuts at: 2983 2983
Size: 5413
PstI C_TGCA'G
Cuts at: 830 2235 2528 2765 830
Size: 1405 293 237 3478
Fragments arranged by size:
3478 1405 293 237
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5413
PvuII CAG'CTG
Cuts at: 2026 2188 2395 2470 2026
Size: 162 207 75 4969
Fragments arranged by size:
4969 207 162 75
SacII CC_GC'GG
Cuts at: 2595 2595
Size: 5413
SalI G'TCGA_C
Cuts at: 1936 2552 2755 1936
Size: 616 203 4594
Fragments arranged by size:
4594 616 203
SapI GCTCTTCn'nnn_
Cuts at: 2093 2093
Size: 5413
SbfI CC_TGCA'GG
Cuts at: 2765 2765
Size: 5413
ScaI AGT'ACT
Cuts at: 3661 5373 3661
Size: 1712 3701
SfcI C'TryA_G
Cuts at: 826 1045 2231 2524 2761 3896 4577 4768 826
Size: 219 1186 293 237 1135 681 191 1471
Fragments arranged by size:
1471 1186 1135 681 293 237 219 191
SfoI GGC'GCC
Cuts at: 2289 2583 2289
Size: 294 5119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5413
SmaI CCC'GGG
Cuts at: 1872 2740 1872
Size: 868 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2105 3517 4418 4680 820
Size: 197 926 162 1412 901 262 1553
Fragments arranged by size:
1553 1412 926 901 262 197 162
SnaBI TAC'GTA
Cuts at: 493 3204 493
Size: 2711 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5413
SphI G_CATG'C
Cuts at: 1132 2771 1132
Size: 1639 3774
SspI AAT'ATT
Cuts at: 5 52 3337 5
Size: 47 3285 2081
Fragments arranged by size:
3285 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5413
StyI C'CwwG_G
Cuts at: 513 1065 2004 2852 513
Size: 552 939 848 3074
Fragments arranged by size:
3074 939 848 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3440 3457 3610 3795 4240 325
Size: 1255 1860 17 153 185 445 1498
Fragments arranged by size:
1860 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3659 5371 96
Size: 274 80 33 51 1443 1682 1712 138
Fragments arranged by size:
1712 1682 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2057 2089 2324 2356 5373 5405 2057
Size: 32 235 32 3017 32 2065
Fragments arranged by size:
3017 2065 235 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5413
XbaI T'CTAG_A
Cuts at: 2749 2749
Size: 5413
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5413
XmaI C'CCGG_G
Cuts at: 1870 2738 1870
Size: 868 4545
XmnI GAAnn'nnTTC
Cuts at: 2168 2645 3542 2168
Size: 477 897 4039
Fragments arranged by size:
4039 897 477
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3221 276
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PspOMI PspXI
PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI
XcmI