Restriction map for
pFN21AE5241
(Circular) (Six-base) MAPSORT of: pFN21AE5241.seq Check: 1634 from: 1 to: 5455
pFN21AE5241 5455 bp
With 182 enzymes: SgfI *
December 8, 2014 13:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3265 278
Size: 53 83 186 936 1729 2468
Fragments arranged by size:
2468 1729 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2798 1937
Size: 861 4594
Acc65I G'GTAC_C
Cuts at: 2776 2776
Size: 5455
AclI AA'CG_TT
Cuts at: 1563 3582 3955 1563
Size: 2019 373 3063
Fragments arranged by size:
3063 2019 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3518 4902 5170 1632
Size: 1886 1384 268 1917
Fragments arranged by size:
1917 1886 1384 268
AfeI AGC'GCT
Cuts at: 2081 2081
Size: 5455
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5258
AflIII A'CryG_T
Cuts at: 1203 4354 5331 1203
Size: 3151 977 1327
Fragments arranged by size:
3151 1327 977
AgeI A'CCGG_T
Cuts at: 4286 4286
Size: 5455
AhdI GACnn_n'nnGTC
Cuts at: 4184 4184
Size: 5455
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5455
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5455
AlwNI CAG_nnn'CTG
Cuts at: 3198 4770 3198
Size: 1572 3883
ApaLI G'TGCA_C
Cuts at: 3512 4668 3512
Size: 1156 4299
ApoI r'AATT_y
Cuts at: 1493 2747 2764 2990 3142 5251 1493
Size: 1254 17 226 152 2109 1697
Fragments arranged by size:
2109 1697 1254 226 152 17
AseI AT'TA_AT
Cuts at: 160 1032 4009 160
Size: 872 2977 1606
Fragments arranged by size:
2977 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5455
AvaI C'yCGr_G
Cuts at: 1870 1943 2644 2780 1870
Size: 73 701 136 4545
Fragments arranged by size:
4545 701 136 73
BamHI G'GATC_C
Cuts at: 2785 3152 2785
Size: 367 5088
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2776 4231 618
Size: 325 217 627 989 1455 1842
Fragments arranged by size:
1842 1455 989 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2774 1391
Size: 1383 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4484
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3646 3680 1903
Size: 34 1709 34 3678
Fragments arranged by size:
3678 1709 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3347 4557 3347
Size: 1210 4245
BclI T'GATC_A
Cuts at: 1548 2583 1548
Size: 1035 4420
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4066 136
Size: 107 122 71 631 605 2394 1525
Fragments arranged by size:
2394 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2212 5450 2212
Size: 3238 2217
BlpI GC'TnA_GC
Cuts at: 2872 2872
Size: 5455
Bme1580I G_kGCm'C
Cuts at: 1790 3516 4672 1790
Size: 1726 1156 2573
Fragments arranged by size:
2573 1726 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5455
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3186 4144 442
Size: 416 53 83 399 24 1769 958 1753
Fragments arranged by size:
1769 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5455
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2336 4115 1239
Size: 1097 1779 2579
Fragments arranged by size:
2579 1779 1097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2558 3580 4445 4743 2558
Size: 1022 865 298 3270
Fragments arranged by size:
3270 1022 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2374 4118 882
Size: 617 875 1744 2219
Fragments arranged by size:
2219 1744 875 617
BsaAI yAC'GTr
Cuts at: 493 1204 3246 493
Size: 711 2042 2702
Fragments arranged by size:
2702 2042 711
BsaBI GATnn'nnATC
Cuts at: 5449 5449
Size: 5455
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3262 3644 4279 275
Size: 53 83 186 936 1729 382 635 1451
Fragments arranged by size:
1729 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3888 4286 4560 4707 5118 3888
Size: 398 274 147 411 4225
Fragments arranged by size:
4225 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2309 2339 1178
Size: 30 165 30 906 30 4294
Fragments arranged by size:
4294 906 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5455
BseYI C'CCAG_C
Cuts at: 4658 5154 4658
Size: 496 4959
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4882
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2905 3666 4694 1540
Size: 275 186 904 761 1028 2301
Fragments arranged by size:
2301 1028 904 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2515 3516 3601 4672 2515
Size: 1001 85 1071 3298
Fragments arranged by size:
3298 1071 1001 85
BsmI GAATG_Cn'
Cuts at: 2966 3059 2966
Size: 93 5362
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2515 2774 3516 3601 4672 1391
Size: 399 725 259 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 725 399 259 85
BspEI T'CCGG_A
Cuts at: 5118 5118
Size: 5455
BspHI T'CATG_A
Cuts at: 3344 5074 3344
Size: 1730 3725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2810 844
Size: 1966 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2127 3342 1394
Size: 429 304 1215 3507
Fragments arranged by size:
3507 1215 429 304
BsrDI GCAATG_nn'
Cuts at: 65 1248 2334 3950 4124 65
Size: 1183 1086 1616 174 1396
Fragments arranged by size:
1616 1396 1183 1086 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4099 4286 1700
Size: 110 2289 187 2869
Fragments arranged by size:
2869 2289 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5455
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5455
BssSI C'ACGA_G
Cuts at: 3515 4527 3515
Size: 1012 4443
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5455
BstBI TT'CG_AA
Cuts at: 2085 4259 2085
Size: 2174 3281
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5455
BstYI r'GATC_y
Cuts at: 1972 2031 2212 2785 3152 3537 3554 5006 5092
Size: 59 181 573 367 385 17 1452 86
Cuts at: 5092 5104 5450 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 573 385 367 346 181 86
59 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2052 513
Size: 552 987 3916
Fragments arranged by size:
3916 987 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4716
Fragments arranged by size:
4716 683 56
BtsI GCAGTG_nn'
Cuts at: 2971 3765 3785 2971
Size: 794 20 4641
Fragments arranged by size:
4641 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5455
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2328 2363 529
Size: 35 1764 35 3621
Fragments arranged by size:
3621 1764 35 35
DraI TTT'AAA
Cuts at: 2759 2759
Size: 5455
DraIII CAC_nnn'GTG
Cuts at: 2170 2170
Size: 5455
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4462 809
Size: 507 3146 1802
Fragments arranged by size:
3146 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2611 2902
Size: 54 1425 50 275 18 219 562 291
Cuts at: 2902 3791 5420 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 562 291 275 219 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2902 1537
Size: 275 1090 4090
Fragments arranged by size:
4090 1090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3385 1353
Size: 2032 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2137 4042 4416 4562 387
Size: 1064 138 435 113 1905 374 146 1280
Fragments arranged by size:
1905 1280 1064 435 374 146 138 113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2336 3518 4115 4902 5170 1239
Size: 393 704 1182 597 787 268 1524
Fragments arranged by size:
1524 1182 787 704 597 393 268
EcoRI G'AATT_C
Cuts at: 2764 3142 2764
Size: 378 5077
FspI TGC'GCA
Cuts at: 3961 3961
Size: 5455
HaeII r_GCGC'y
Cuts at: 1678 2083 4602 1678
Size: 405 2519 2531
Fragments arranged by size:
2531 2519 405
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4096 4128 4170 4202 1373
Size: 32 135 32 2524 32 42 32 2626
Fragments arranged by size:
2626 2524 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2799 3045 669
Size: 988 281 861 246 3079
Fragments arranged by size:
3079 988 861 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5455
HpaI GTT'AAC
Cuts at: 3045 3045
Size: 5455
KpnI G_GTAC'C
Cuts at: 2780 2780
Size: 5455
MfeI C'AATT_G
Cuts at: 3054 3054
Size: 5455
MluI A'CGCG_T
Cuts at: 5331 5331
Size: 5455
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4569 4753 1305
Size: 3264 184 2007
Fragments arranged by size:
3264 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2613 10
Size: 54 1425 343 781 2852
Fragments arranged by size:
2852 1425 781 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3413 3772 3931 518
Size: 648 2247 359 159 2042
Fragments arranged by size:
2247 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2871 3548 4696 4941 1606
Size: 1265 677 1148 245 2120
Fragments arranged by size:
2120 1265 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4903
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5455
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5455
NotI GC'GGCC_GC
Cuts at: 2902 2902
Size: 5455
NspI r_CATG'y
Cuts at: 1132 1229 2813 4358 1132
Size: 97 1584 1545 2229
Fragments arranged by size:
2229 1584 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5455
PciI A'CATG_T
Cuts at: 4354 4354
Size: 5455
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5455
PmeI GTTT'AAAC
Cuts at: 2759 2759
Size: 5455
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 5037 5069 3524
Size: 32 1481 32 3910
Fragments arranged by size:
3910 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5455
PsiI TTA'TAA
Cuts at: 3025 3025
Size: 5455
PstI C_TGCA'G
Cuts at: 830 2160 2807 830
Size: 1330 647 3478
Fragments arranged by size:
3478 1330 647
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5455
SalI G'TCGA_C
Cuts at: 1936 2797 1936
Size: 861 4594
SbfI CC_TGCA'GG
Cuts at: 2807 2807
Size: 5455
ScaI AGT'ACT
Cuts at: 2712 3703 5415 2712
Size: 991 1712 2752
Fragments arranged by size:
2752 1712 991
SfcI C'TryA_G
Cuts at: 826 1045 2156 2803 3938 4619 4810 826
Size: 219 1111 647 1135 681 191 1471
Fragments arranged by size:
1471 1135 1111 681 647 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5455
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5455
SmaI CCC'GGG
Cuts at: 1872 2646 2782 1872
Size: 774 136 4545
Fragments arranged by size:
4545 774 136
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2573 3559 4460 4722 820
Size: 197 926 630 986 901 262 1553
Fragments arranged by size:
1553 986 926 901 630 262 197
SnaBI TAC'GTA
Cuts at: 493 3246 493
Size: 2753 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5455
SphI G_CATG'C
Cuts at: 1132 2813 1132
Size: 1681 3774
SspI AAT'ATT
Cuts at: 5 52 3379 5
Size: 47 3327 2081
Fragments arranged by size:
3327 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5455
StyI C'CwwG_G
Cuts at: 513 1065 2894 513
Size: 552 1829 3074
Fragments arranged by size:
3074 1829 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3482 3499 3652 3837 4282 325
Size: 1255 1902 17 153 185 445 1498
Fragments arranged by size:
1902 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2450 2710 3701
Size: 274 80 33 51 1443 473 260 991
Cuts at: 3701 5413 96
Size: 1712 138
Fragments arranged by size:
1712 1443 991 473 274 260 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2053 2085 5415 5447 2053
Size: 32 3330 32 2061
Fragments arranged by size:
3330 2061 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5455
XbaI T'CTAG_A
Cuts at: 2791 2791
Size: 5455
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2050 2050
Size: 5455
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5455
XmaI C'CCGG_G
Cuts at: 1870 2644 2780 1870
Size: 774 136 4545
Fragments arranged by size:
4545 774 136
XmnI GAAnn'nnTTC
Cuts at: 2081 3584 2081
Size: 1503 3952
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3263 276
Size: 53 83 186 936 1729 2468
Fragments arranged by size:
2468 1729 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI