Restriction map for
pFN21AE5240
(Circular) (Six-base) MAPSORT of: pFN21AE5240.seq Check: 318 from: 1 to: 5458
pFN21AE5240 5458 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3268 278
Size: 53 83 186 936 1732 2468
Fragments arranged by size:
2468 1732 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2801 1937
Size: 864 4594
Acc65I G'GTAC_C
Cuts at: 2779 2779
Size: 5458
AclI AA'CG_TT
Cuts at: 1563 3585 3958 1563
Size: 2022 373 3063
Fragments arranged by size:
3063 2022 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2695 3521 4905 5173 1632
Size: 1063 826 1384 268 1917
Fragments arranged by size:
1917 1384 1063 826 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5261
AflIII A'CryG_T
Cuts at: 1203 4357 5334 1203
Size: 3154 977 1327
Fragments arranged by size:
3154 1327 977
AgeI A'CCGG_T
Cuts at: 4289 4289
Size: 5458
AhdI GACnn_n'nnGTC
Cuts at: 4187 4187
Size: 5458
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5458
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5458
AlwNI CAG_nnn'CTG
Cuts at: 3201 4773 3201
Size: 1572 3886
ApaLI G'TGCA_C
Cuts at: 3515 4671 3515
Size: 1156 4302
ApoI r'AATT_y
Cuts at: 1493 2767 2993 3145 5254 1493
Size: 1274 226 152 2109 1697
Fragments arranged by size:
2109 1697 1274 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4012 160
Size: 872 2980 1606
Fragments arranged by size:
2980 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
AvaI C'yCGr_G
Cuts at: 1870 1943 2586 2605 2783 1870
Size: 73 643 19 178 4545
Fragments arranged by size:
4545 643 178 73 19
BamHI G'GATC_C
Cuts at: 2424 2788 3155 2424
Size: 364 367 4727
Fragments arranged by size:
4727 367 364
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2081 2779 4234 618
Size: 325 217 627 294 698 1455 1842
Fragments arranged by size:
1842 1455 698 627 325 294 217
BanII G_rGCy'C
Cuts at: 1391 2777 1391
Size: 1386 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2097 928
Size: 971 198 4289
Fragments arranged by size:
4289 971 198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3649 3683 1903
Size: 34 1712 34 3678
Fragments arranged by size:
3678 1712 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3350 4560 3350
Size: 1210 4248
BclI T'GATC_A
Cuts at: 1548 2334 1548
Size: 786 4672
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4069 136
Size: 107 122 71 631 605 2397 1525
Fragments arranged by size:
2397 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5453 5453
Size: 5458
BlpI GC'TnA_GC
Cuts at: 2875 2875
Size: 5458
Bme1580I G_kGCm'C
Cuts at: 1790 3519 4675 1790
Size: 1729 1156 2573
Fragments arranged by size:
2573 1729 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5458
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2608 2701 3189
Size: 416 53 83 399 24 1191 93 488
Cuts at: 3189 4147 442
Size: 958 1753
Fragments arranged by size:
1753 1191 958 488 416 399 93 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5458
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2183 2183
Size: 5458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2134 4118 1239
Size: 895 1984 2579
Fragments arranged by size:
2579 1984 895
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3583 4448 4746 3583
Size: 865 298 4295
Fragments arranged by size:
4295 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2616 4121 882
Size: 617 1117 1505 2219
Fragments arranged by size:
2219 1505 1117 617
BsaAI yAC'GTr
Cuts at: 493 1204 3249 493
Size: 711 2045 2702
Fragments arranged by size:
2702 2045 711
BsaBI GATnn'nnATC
Cuts at: 2036 2581 5452 2036
Size: 545 2871 2042
Fragments arranged by size:
2871 2042 545
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3265 3647 4282 275
Size: 53 83 186 936 1732 382 635 1451
Fragments arranged by size:
1732 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3891 4289 4563 4710 5121 3891
Size: 398 274 147 411 4228
Fragments arranged by size:
4228 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5233
Fragments arranged by size:
5233 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2159 2336 1186
Size: 973 177 4308
Fragments arranged by size:
4308 973 177
BseYI C'CCAG_C
Cuts at: 4661 5157 4661
Size: 496 4962
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4885
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2104 2908 3669 4697 1540
Size: 275 186 103 804 761 1028 2301
Fragments arranged by size:
2301 1028 804 761 275 186 103
BsiHKAI G_wGCw'C
Cuts at: 3519 3604 4675 3519
Size: 85 1071 4302
Fragments arranged by size:
4302 1071 85
BsmI GAATG_Cn'
Cuts at: 2969 3062 2969
Size: 93 5365
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2777 3519 3604 4675 1391
Size: 399 987 742 85 1071 2174
Fragments arranged by size:
2174 1071 987 742 399 85
BspEI T'CCGG_A
Cuts at: 5121 5121
Size: 5458
BspHI T'CATG_A
Cuts at: 3347 5077 3347
Size: 1730 3728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2813 844
Size: 1969 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3345 1394
Size: 429 1522 3507
Fragments arranged by size:
3507 1522 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2127 3953 4127 65
Size: 1183 879 1826 174 1396
Fragments arranged by size:
1826 1396 1183 879 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2496 4102 4289 1700
Size: 110 686 1606 187 2869
Fragments arranged by size:
2869 1606 686 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5458
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5458
BssSI C'ACGA_G
Cuts at: 3518 4530 3518
Size: 1012 4446
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5458
BstBI TT'CG_AA
Cuts at: 2506 4262 2506
Size: 1756 3702
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5458
BstYI r'GATC_y
Cuts at: 1972 2161 2424 2788 3155 3540 3557 5009 5095
Size: 189 263 364 367 385 17 1452 86
Cuts at: 5095 5107 5453 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 364 346 263 189
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4906
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2023 487
Size: 683 56 797 3922
Fragments arranged by size:
3922 797 683 56
BtsI GCAGTG_nn'
Cuts at: 2974 3768 3788 2974
Size: 794 20 4644
Fragments arranged by size:
4644 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5458
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5423
DraI TTT'AAA
Cuts at: 2762 2762
Size: 5458
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2108 4465 809
Size: 507 792 2357 1802
Fragments arranged by size:
2357 1802 792 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2184 2905 3794
Size: 54 1425 50 275 18 354 721 889
Cuts at: 3794 5423 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 721 354 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2905 1537
Size: 275 1093 4090
Fragments arranged by size:
4090 1093 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2639 3388 1353
Size: 1286 749 3423
Fragments arranged by size:
3423 1286 749
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4045 4419 4565 387
Size: 1064 138 2456 374 146 1280
Fragments arranged by size:
2456 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2134 2695 3521 4118 4905 5173 1239
Size: 393 502 561 826 597 787 268 1524
Fragments arranged by size:
1524 826 787 597 561 502 393 268
EcoRI G'AATT_C
Cuts at: 2767 3145 2767
Size: 378 5080
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 5458
FspI TGC'GCA
Cuts at: 3964 3964
Size: 5458
HaeII r_GCGC'y
Cuts at: 1678 4605 1678
Size: 2927 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2602 2634 4099 4131 4173
Size: 32 135 32 1030 32 1465 32 42
Cuts at: 4173 4205 1373
Size: 32 2626
Fragments arranged by size:
2626 1465 1030 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2802 3048 669
Size: 988 281 864 246 3079
Fragments arranged by size:
3079 988 864 281 246
HindIII A'AGCT_T
Cuts at: 748 2340 748
Size: 1592 3866
HpaI GTT'AAC
Cuts at: 3048 3048
Size: 5458
KpnI G_GTAC'C
Cuts at: 2783 2783
Size: 5458
MfeI C'AATT_G
Cuts at: 3057 3057
Size: 5458
MluI A'CGCG_T
Cuts at: 5334 5334
Size: 5458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4572 4756 1305
Size: 3267 184 2007
Fragments arranged by size:
3267 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2186 10
Size: 54 1425 343 354 3282
Fragments arranged by size:
3282 1425 354 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3416 3775 3934 518
Size: 648 2250 359 159 2042
Fragments arranged by size:
2250 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2874 3551 4699 4944 1606
Size: 1268 677 1148 245 2120
Fragments arranged by size:
2120 1268 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4906
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5458
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5458
NotI GC'GGCC_GC
Cuts at: 2905 2905
Size: 5458
NspI r_CATG'y
Cuts at: 1132 1229 2816 4361 1132
Size: 97 1587 1545 2229
Fragments arranged by size:
2229 1587 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5458
PciI A'CATG_T
Cuts at: 4357 4357
Size: 5458
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2676 1108
Size: 1568 3890
PmeI GTTT'AAAC
Cuts at: 2762 2762
Size: 5458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3527 3559 5040 5072 3527
Size: 32 1481 32 3913
Fragments arranged by size:
3913 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5458
PsiI TTA'TAA
Cuts at: 3028 3028
Size: 5458
PspXI vC'TCGA_Gb
Cuts at: 2605 2605
Size: 5458
PstI C_TGCA'G
Cuts at: 830 2810 830
Size: 1980 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5458
SalI G'TCGA_C
Cuts at: 1936 2800 1936
Size: 864 4594
SbfI CC_TGCA'GG
Cuts at: 2810 2810
Size: 5458
ScaI AGT'ACT
Cuts at: 3706 5418 3706
Size: 1712 3746
SfcI C'TryA_G
Cuts at: 826 1045 2545 2806 3941 4622 4813 826
Size: 219 1500 261 1135 681 191 1471
Fragments arranged by size:
1500 1471 1135 681 261 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5458
SmaI CCC'GGG
Cuts at: 1872 2785 1872
Size: 913 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2605 3562 4463 4725 820
Size: 197 926 662 957 901 262 1553
Fragments arranged by size:
1553 957 926 901 662 262 197
SnaBI TAC'GTA
Cuts at: 493 3249 493
Size: 2756 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5458
SphI G_CATG'C
Cuts at: 1132 2816 1132
Size: 1684 3774
SspI AAT'ATT
Cuts at: 5 52 3382 5
Size: 47 3330 2081
Fragments arranged by size:
3330 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5458
StyI C'CwwG_G
Cuts at: 513 1065 2043 2205 2897 513
Size: 552 978 162 692 3074
Fragments arranged by size:
3074 978 692 552 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3485 3502 3655 3840 4285 325
Size: 1255 1905 17 153 185 445 1498
Fragments arranged by size:
1905 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3704 5416 96
Size: 274 80 33 51 1443 1727 1712 138
Fragments arranged by size:
1727 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2121 2153 5418 5450 2121
Size: 32 3265 32 2129
Fragments arranged by size:
3265 2129 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2276 1316
Size: 960 4498
XbaI T'CTAG_A
Cuts at: 2794 2794
Size: 5458
XhoI C'TCGA_G
Cuts at: 1943 2605 1943
Size: 662 4796
XmaI C'CCGG_G
Cuts at: 1870 2783 1870
Size: 913 4545
XmnI GAAnn'nnTTC
Cuts at: 3587 3587
Size: 5458
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3266 276
Size: 53 83 186 936 1732 2468
Fragments arranged by size:
2468 1732 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI