Restriction map for
pFN21AE5239
(Circular) (Six-base) MAPSORT of: pFN21AE5239.seq Check: 5207 from: 1 to: 5473
pFN21AE5239 5473 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2294 3283 278
Size: 53 83 186 936 758 989 2468
Fragments arranged by size:
2468 989 936 758 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2816 1937
Size: 879 4594
Acc65I G'GTAC_C
Cuts at: 2794 2794
Size: 5473
AclI AA'CG_TT
Cuts at: 1563 3600 3973 1563
Size: 2037 373 3063
Fragments arranged by size:
3063 2037 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2255 3536 4920 5188 1632
Size: 623 1281 1384 268 1917
Fragments arranged by size:
1917 1384 1281 623 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5276
AflIII A'CryG_T
Cuts at: 1203 2670 4372 5349 1203
Size: 1467 1702 977 1327
Fragments arranged by size:
1702 1467 1327 977
AgeI A'CCGG_T
Cuts at: 4304 4304
Size: 5473
AhdI GACnn_n'nnGTC
Cuts at: 4202 4202
Size: 5473
AleI CACnn'nnGTG
Cuts at: 1166 2717 1166
Size: 1551 3922
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2195 1184
Size: 1011 4462
AlwNI CAG_nnn'CTG
Cuts at: 2098 2350 3216 4788 2098
Size: 252 866 1572 2783
Fragments arranged by size:
2783 1572 866 252
ApaLI G'TGCA_C
Cuts at: 3530 4686 3530
Size: 1156 4317
ApoI r'AATT_y
Cuts at: 1493 2568 2782 3008 3160 5269 1493
Size: 1075 214 226 152 2109 1697
Fragments arranged by size:
2109 1697 1075 226 214 152
AseI AT'TA_AT
Cuts at: 160 1032 4027 160
Size: 872 2995 1606
Fragments arranged by size:
2995 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5473
AvaI C'yCGr_G
Cuts at: 1870 1943 2798 1870
Size: 73 855 4545
Fragments arranged by size:
4545 855 73
BamHI G'GATC_C
Cuts at: 2520 2803 3170 2520
Size: 283 367 4823
Fragments arranged by size:
4823 367 283
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2794 4249 618
Size: 325 217 627 1007 1455 1842
Fragments arranged by size:
1842 1455 1007 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2375 2792 1391
Size: 984 417 4072
Fragments arranged by size:
4072 984 417
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2023 2603 928
Size: 971 124 580 3798
Fragments arranged by size:
3798 971 580 124
BbvCI CC'TCA_GC
Cuts at: 2168 2168
Size: 5473
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3664 3698 1903
Size: 34 1727 34 3678
Fragments arranged by size:
3678 1727 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3365 4575 3365
Size: 1210 4263
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5473
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2199 2379 4084
Size: 107 122 71 631 605 527 180 1705
Cuts at: 4084 136
Size: 1525
Fragments arranged by size:
1705 1525 631 605 527 180 122 107
71
BglII A'GATC_T
Cuts at: 5468 5468
Size: 5473
BlpI GC'TnA_GC
Cuts at: 2890 2890
Size: 5473
Bme1580I G_kGCm'C
Cuts at: 1790 2085 2678 3534 4690 1790
Size: 295 593 856 1156 2573
Fragments arranged by size:
2573 1156 856 593 295
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5473
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2025 2056 3204
Size: 416 53 83 399 24 608 31 1148
Cuts at: 3204 4162 442
Size: 958 1753
Fragments arranged by size:
1753 1148 958 608 416 399 83 53
31 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2262 4133 1239
Size: 1023 1871 2579
Fragments arranged by size:
2579 1871 1023
Bpu10I CC'TnA_GC
Cuts at: 2168 2168
Size: 5473
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2149 3598 4463 4761 2149
Size: 1449 865 298 2861
Fragments arranged by size:
2861 1449 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4136 882
Size: 617 2637 2219
Fragments arranged by size:
2637 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3264 493
Size: 711 2060 2702
Fragments arranged by size:
2702 2060 711
BsaBI GATnn'nnATC
Cuts at: 5467 5467
Size: 5473
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2291 3280 3662 4297
Size: 53 83 186 936 758 989 382 635
Cuts at: 4297 275
Size: 1451
Fragments arranged by size:
1451 989 936 758 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3906 4304 4578 4725 5136 3906
Size: 398 274 147 411 4243
Fragments arranged by size:
4243 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5248
Fragments arranged by size:
5248 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2259 1186
Size: 1073 4400
BseYI C'CCAG_C
Cuts at: 2343 4676 5172 2343
Size: 2333 496 2644
Fragments arranged by size:
2644 2333 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2387 2527 1163
Size: 573 651 140 4109
Fragments arranged by size:
4109 651 573 140
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2923 3684 4712 1540
Size: 275 186 922 761 1028 2301
Fragments arranged by size:
2301 1028 922 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3534 3619 4690 3534
Size: 85 1071 4317
Fragments arranged by size:
4317 1071 85
BsmI GAATG_Cn'
Cuts at: 2202 2984 3077 2202
Size: 782 93 4598
Fragments arranged by size:
4598 782 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2393 2393
Size: 5473
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2375 2678 2792 3534 3619 4690
Size: 399 295 290 303 114 742 85 1071
Cuts at: 4690 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 303 295 290 114
85
BspEI T'CCGG_A
Cuts at: 5136 5136
Size: 5473
BspHI T'CATG_A
Cuts at: 3362 5092 3362
Size: 1730 3743
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2828 844
Size: 1984 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2221 3360 1394
Size: 429 398 1139 3507
Fragments arranged by size:
3507 1139 429 398
BsrDI GCAATG_nn'
Cuts at: 65 1248 2179 3968 4142 65
Size: 1183 931 1789 174 1396
Fragments arranged by size:
1789 1396 1183 931 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4117 4304 1700
Size: 110 2307 187 2869
Fragments arranged by size:
2869 2307 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5473
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5473
BssSI C'ACGA_G
Cuts at: 3533 4545 3533
Size: 1012 4461
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2673 794
Size: 1879 3594
BstBI TT'CG_AA
Cuts at: 4277 4277
Size: 5473
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5473
BstXI CCAn_nnnn'nTGG
Cuts at: 2555 2555
Size: 5473
BstYI r'GATC_y
Cuts at: 1972 2520 2631 2803 3170 3555 3572 5024 5110
Size: 548 111 172 367 385 17 1452 86
Cuts at: 5110 5122 5468 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 548 385 367 346 172 111
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2055 2746 513
Size: 552 990 691 3240
Fragments arranged by size:
3240 990 691 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4734
Fragments arranged by size:
4734 683 56
BtsI GCAGTG_nn'
Cuts at: 2481 2989 3783 3803 2481
Size: 508 794 20 4151
Fragments arranged by size:
4151 794 508 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5473
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2125 2160 529
Size: 35 1561 35 3842
Fragments arranged by size:
3842 1561 35 35
DraI TTT'AAA
Cuts at: 2777 2777
Size: 5473
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2330 4480 809
Size: 507 1014 2150 1802
Fragments arranged by size:
2150 1802 1014 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2380 2920 3809
Size: 54 1425 50 275 18 550 540 889
Cuts at: 3809 5438 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 550 540 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2920 1537
Size: 275 1108 4090
Fragments arranged by size:
4090 1108 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3403 1353
Size: 2050 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4060 4434 4580 387
Size: 1064 138 2471 374 146 1280
Fragments arranged by size:
2471 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2255 2262 3536 4133 4920 5188 1239
Size: 393 623 7 1274 597 787 268 1524
Fragments arranged by size:
1524 1274 787 623 597 393 268 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2244 2244
Size: 5473
EcoO109I rG'GnC_Cy
Cuts at: 2020 2191 2020
Size: 171 5302
EcoRI G'AATT_C
Cuts at: 2782 3160 2782
Size: 378 5095
FspI TGC'GCA
Cuts at: 3979 3979
Size: 5473
HaeII r_GCGC'y
Cuts at: 1678 4620 1678
Size: 2942 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4114 4146 4188 4220 1373
Size: 32 135 32 2542 32 42 32 2626
Fragments arranged by size:
2626 2542 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2734 2817 3063 669
Size: 988 281 796 83 246 3079
Fragments arranged by size:
3079 988 796 281 246 83
HindIII A'AGCT_T
Cuts at: 748 2152 748
Size: 1404 4069
HpaI GTT'AAC
Cuts at: 2734 3063 2734
Size: 329 5144
KpnI G_GTAC'C
Cuts at: 2798 2798
Size: 5473
MfeI C'AATT_G
Cuts at: 3072 3072
Size: 5473
MluI A'CGCG_T
Cuts at: 5349 5349
Size: 5473
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4587 4771 1305
Size: 3282 184 2007
Fragments arranged by size:
3282 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2382 10
Size: 54 1425 343 550 3101
Fragments arranged by size:
3101 1425 550 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2717 3431 3790 3949 518
Size: 648 1551 714 359 159 2042
Fragments arranged by size:
2042 1551 714 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2347 2889 3566 4714 4959 1606
Size: 741 542 677 1148 245 2120
Fragments arranged by size:
2120 1148 741 677 542 245
NcoI C'CATG_G
Cuts at: 513 1065 2055 513
Size: 552 990 3931
Fragments arranged by size:
3931 990 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5473
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5473
NotI GC'GGCC_GC
Cuts at: 2920 2920
Size: 5473
NspI r_CATG'y
Cuts at: 1132 1229 2740 2831 4376 1132
Size: 97 1511 91 1545 2229
Fragments arranged by size:
2229 1545 1511 97 91
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5473
PciI A'CATG_T
Cuts at: 4372 4372
Size: 5473
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2056 2350 1108
Size: 948 294 4231
Fragments arranged by size:
4231 948 294
PfoI T'CCnGG_A
Cuts at: 2223 2223
Size: 5473
PmeI GTTT'AAAC
Cuts at: 2777 2777
Size: 5473
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3542 3574 5055 5087 3542
Size: 32 1481 32 3928
Fragments arranged by size:
3928 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5473
PsiI TTA'TAA
Cuts at: 3043 3043
Size: 5473
PstI C_TGCA'G
Cuts at: 830 2488 2825 830
Size: 1658 337 3478
Fragments arranged by size:
3478 1658 337
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5473
PvuII CAG'CTG
Cuts at: 2347 2347
Size: 5473
SalI G'TCGA_C
Cuts at: 1936 2815 1936
Size: 879 4594
SbfI CC_TGCA'GG
Cuts at: 2825 2825
Size: 5473
ScaI AGT'ACT
Cuts at: 3721 5433 3721
Size: 1712 3761
SexAI A'CCwGG_T
Cuts at: 2526 2526
Size: 5473
SfcI C'TryA_G
Cuts at: 826 1045 2484 2753 2821 3956 4637 4828 826
Size: 219 1439 269 68 1135 681 191 1471
Fragments arranged by size:
1471 1439 1135 681 269 219 191 68
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5473
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5473
SmaI CCC'GGG
Cuts at: 1872 2800 1872
Size: 928 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2128 3577 4478 4740 820
Size: 197 926 185 1449 901 262 1553
Fragments arranged by size:
1553 1449 926 901 262 197 185
SnaBI TAC'GTA
Cuts at: 493 3264 493
Size: 2771 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5473
SphI G_CATG'C
Cuts at: 1132 2831 1132
Size: 1699 3774
SspI AAT'ATT
Cuts at: 5 52 3397 5
Size: 47 3345 2081
Fragments arranged by size:
3345 2081 47
StuI AGG'CCT
Cuts at: 1517 2073 1517
Size: 556 4917
StyI C'CwwG_G
Cuts at: 513 1065 2055 2266 2533 2912 513
Size: 552 990 211 267 379 3074
Fragments arranged by size:
3074 990 552 379 267 211
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2561 3500 3517 3670 3855 4300 325
Size: 1255 981 939 17 153 185 445 1498
Fragments arranged by size:
1498 1255 981 939 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3719 5431 96
Size: 274 80 33 51 1443 1742 1712 138
Fragments arranged by size:
1742 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5433 5465 5433
Size: 32 5441
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5473
XbaI T'CTAG_A
Cuts at: 2809 2809
Size: 5473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2531 2531
Size: 5473
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5473
XmaI C'CCGG_G
Cuts at: 1870 2798 1870
Size: 928 4545
XmnI GAAnn'nnTTC
Cuts at: 2214 3602 2214
Size: 1388 4085
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2292 3281 276
Size: 53 83 186 936 758 989 2468
Fragments arranged by size:
2468 989 936 758 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI