Restriction map for
pFN21AE5235
(Circular) (Six-base) MAPSORT of: pFN21AE5235.seq Check: 5607 from: 1 to: 5293
pFN21AE5235 5293 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3103 278
Size: 53 83 186 936 1567 2468
Fragments arranged by size:
2468 1567 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2636 1937
Size: 699 4594
Acc65I G'GTAC_C
Cuts at: 2614 2614
Size: 5293
AclI AA'CG_TT
Cuts at: 1563 3420 3793 1563
Size: 1857 373 3063
Fragments arranged by size:
3063 1857 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2006 2399 2459 3356 4740 5008 1632
Size: 374 393 60 897 1384 268 1917
Fragments arranged by size:
1917 1384 897 393 374 268 60
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5096
AflIII A'CryG_T
Cuts at: 1203 2358 4192 5169 1203
Size: 1155 1834 977 1327
Fragments arranged by size:
1834 1327 1155 977
AgeI A'CCGG_T
Cuts at: 4124 4124
Size: 5293
AhdI GACnn_n'nnGTC
Cuts at: 4022 4022
Size: 5293
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5293
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5293
AlwNI CAG_nnn'CTG
Cuts at: 3036 4608 3036
Size: 1572 3721
ApaLI G'TGCA_C
Cuts at: 3350 4506 3350
Size: 1156 4137
ApoI r'AATT_y
Cuts at: 1493 2602 2828 2980 5089 1493
Size: 1109 226 152 2109 1697
Fragments arranged by size:
2109 1697 1109 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3847 160
Size: 872 2815 1606
Fragments arranged by size:
2815 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5293
AvaI C'yCGr_G
Cuts at: 1870 1943 2181 2618 1870
Size: 73 238 437 4545
Fragments arranged by size:
4545 437 238 73
BamHI G'GATC_C
Cuts at: 2623 2990 2623
Size: 367 4926
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2614 4069 618
Size: 325 217 627 827 1455 1842
Fragments arranged by size:
1842 1455 827 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2188 2612 1391
Size: 797 424 4072
Fragments arranged by size:
4072 797 424
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4322
BbvCI CC'TCA_GC
Cuts at: 2481 2481
Size: 5293
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3484 3518 1903
Size: 34 1547 34 3678
Fragments arranged by size:
3678 1547 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3185 4395 3185
Size: 1210 4083
BclI T'GATC_A
Cuts at: 1548 2151 1548
Size: 603 4690
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2220 3904 136
Size: 107 122 71 631 605 548 1684 1525
Fragments arranged by size:
1684 1525 631 605 548 122 107 71
BglII A'GATC_T
Cuts at: 5288 5288
Size: 5293
BlpI GC'TnA_GC
Cuts at: 2710 2710
Size: 5293
Bme1580I G_kGCm'C
Cuts at: 1790 3354 4510 1790
Size: 1564 1156 2573
Fragments arranged by size:
2573 1564 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5293
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3024 3982 442
Size: 416 53 83 399 24 1607 958 1753
Fragments arranged by size:
1753 1607 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5293
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2519 3953 1239
Size: 1280 1434 2579
Fragments arranged by size:
2579 1434 1280
Bpu10I CC'TnA_GC
Cuts at: 2015 2481 2015
Size: 466 4827
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3418 4283 4581 3418
Size: 865 298 4130
Fragments arranged by size:
4130 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2081 2576 3956 882
Size: 617 582 495 1380 2219
Fragments arranged by size:
2219 1380 617 582 495
BsaAI yAC'GTr
Cuts at: 493 1204 3084 493
Size: 711 1880 2702
Fragments arranged by size:
2702 1880 711
BsaBI GATnn'nnATC
Cuts at: 5287 5287
Size: 5293
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3100 3482 4117 275
Size: 53 83 186 936 1567 382 635 1451
Fragments arranged by size:
1567 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2127 3726 4124 4398 4545 4956 2127
Size: 1599 398 274 147 411 2464
Fragments arranged by size:
2464 1599 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5068
Fragments arranged by size:
5068 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2308 2564 1186
Size: 1122 256 3915
Fragments arranged by size:
3915 1122 256
BseYI C'CCAG_C
Cuts at: 4496 4992 4496
Size: 496 4797
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4720
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2353 2743 3504 4532 1540
Size: 275 186 352 390 761 1028 2301
Fragments arranged by size:
2301 1028 761 390 352 275 186
BsiHKAI G_wGCw'C
Cuts at: 3354 3439 4510 3354
Size: 85 1071 4137
Fragments arranged by size:
4137 1071 85
BsmI GAATG_Cn'
Cuts at: 2274 2804 2897 2274
Size: 530 93 4670
Fragments arranged by size:
4670 530 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2188 2612 3354 3439 4510 1391
Size: 399 398 424 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 424 399 398 85
BspEI T'CCGG_A
Cuts at: 2127 4956 2127
Size: 2829 2464
BspHI T'CATG_A
Cuts at: 3182 4912 3182
Size: 1730 3563
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2481 2648 844
Size: 1637 167 3489
Fragments arranged by size:
3489 1637 167
BsrBI CCG'CTC
Cuts at: 1394 1823 3180 1394
Size: 429 1357 3507
Fragments arranged by size:
3507 1357 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3788 3962 65
Size: 1183 2540 174 1396
Fragments arranged by size:
2540 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2313 3937 4124 1700
Size: 110 503 1624 187 2869
Fragments arranged by size:
2869 1624 503 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5293
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5293
BssSI C'ACGA_G
Cuts at: 3353 4365 3353
Size: 1012 4281
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5293
BstBI TT'CG_AA
Cuts at: 4097 4097
Size: 5293
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5293
BstYI r'GATC_y
Cuts at: 1972 2124 2623 2990 3375 3392 4844 4930 4942
Size: 152 499 367 385 17 1452 86 12
Cuts at: 4942 5288 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 499 385 367 346 152 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4741
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4554
Fragments arranged by size:
4554 683 56
BtsI GCAGTG_nn'
Cuts at: 2809 3603 3623 2809
Size: 794 20 4479
Fragments arranged by size:
4479 794 20
ClaI AT'CG_AT
Cuts at: 1554 2349 1554
Size: 795 4498
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5258
DraI TTT'AAA
Cuts at: 2597 2597
Size: 5293
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4300 809
Size: 507 2984 1802
Fragments arranged by size:
2984 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2740 3629 5258
Size: 54 1425 50 275 18 910 889 1629
Cuts at: 5258 8
Size: 43
Fragments arranged by size:
1629 1425 910 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2740 1537
Size: 275 928 4090
Fragments arranged by size:
4090 928 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2021 2520 2574 3223 1353
Size: 668 499 54 649 3423
Fragments arranged by size:
3423 668 649 499 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3880 4254 4400 387
Size: 1064 138 2291 374 146 1280
Fragments arranged by size:
2291 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2006 2399 2459 2519 3356 3953 4740
Size: 393 374 393 60 60 837 597 787
Cuts at: 4740 5008 1239
Size: 268 1524
Fragments arranged by size:
1524 837 787 597 393 393 374 268
60 60
EcoRI G'AATT_C
Cuts at: 2602 2980 2602
Size: 378 4915
FspI TGC'GCA
Cuts at: 3799 3799
Size: 5293
HaeII r_GCGC'y
Cuts at: 1678 2521 2533 4440 1678
Size: 843 12 1907 2531
Fragments arranged by size:
2531 1907 843 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3934 3966 4008 4040 1373
Size: 32 135 32 2362 32 42 32 2626
Fragments arranged by size:
2626 2362 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2637 2883 669
Size: 988 281 699 246 3079
Fragments arranged by size:
3079 988 699 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5293
HpaI GTT'AAC
Cuts at: 2883 2883
Size: 5293
KpnI G_GTAC'C
Cuts at: 2618 2618
Size: 5293
MfeI C'AATT_G
Cuts at: 2892 2892
Size: 5293
MluI A'CGCG_T
Cuts at: 5169 5169
Size: 5293
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4407 4591 1305
Size: 3102 184 2007
Fragments arranged by size:
3102 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3471
Fragments arranged by size:
3471 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2288 3251 3610 3769 518
Size: 648 843 279 963 359 159 2042
Fragments arranged by size:
2042 963 843 648 359 279 159
MspA1I CmG'CkG
Cuts at: 1606 2052 2709 3386 4534 4779 1606
Size: 446 657 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 657 446 245
NaeI GCC'GGC
Cuts at: 2315 2315
Size: 5293
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4741
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5293
NgoMIV G'CCGG_C
Cuts at: 2313 2313
Size: 5293
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5293
NotI GC'GGCC_GC
Cuts at: 2740 2740
Size: 5293
NspI r_CATG'y
Cuts at: 1132 1229 2362 2651 4196 1132
Size: 97 1133 289 1545 2229
Fragments arranged by size:
2229 1545 1133 289 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5293
PciI A'CATG_T
Cuts at: 2358 4192 2358
Size: 1834 3459
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5293
PmeI GTTT'AAAC
Cuts at: 2597 2597
Size: 5293
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3362 3394 4875 4907 3362
Size: 32 1481 32 3748
Fragments arranged by size:
3748 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5293
PsiI TTA'TAA
Cuts at: 2863 2863
Size: 5293
PstI C_TGCA'G
Cuts at: 830 2645 830
Size: 1815 3478
PvuI CG_AT'CG
Cuts at: 2001 2353 2001
Size: 352 4941
SalI G'TCGA_C
Cuts at: 1936 2635 1936
Size: 699 4594
SapI GCTCTTCn'nnn_
Cuts at: 2520 2520
Size: 5293
SbfI CC_TGCA'GG
Cuts at: 2645 2645
Size: 5293
ScaI AGT'ACT
Cuts at: 3541 5253 3541
Size: 1712 3581
SfcI C'TryA_G
Cuts at: 826 1045 2641 3776 4457 4648 826
Size: 219 1596 1135 681 191 1471
Fragments arranged by size:
1596 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5293
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5293
SmaI CCC'GGG
Cuts at: 1872 2183 2620 1872
Size: 311 437 4545
Fragments arranged by size:
4545 437 311
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3397 4298 4560 820
Size: 197 926 1454 901 262 1553
Fragments arranged by size:
1553 1454 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3084 493
Size: 2591 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5293
SphI G_CATG'C
Cuts at: 1132 2651 1132
Size: 1519 3774
SspI AAT'ATT
Cuts at: 5 52 3217 5
Size: 47 3165 2081
Fragments arranged by size:
3165 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5293
StyI C'CwwG_G
Cuts at: 513 1065 2205 2502 2571 2732 513
Size: 552 1140 297 69 161 3074
Fragments arranged by size:
3074 1140 552 297 161 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3320 3337 3490 3675 4120 325
Size: 1255 1740 17 153 185 445 1498
Fragments arranged by size:
1740 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3539 5251 96
Size: 274 80 33 51 1443 1562 1712 138
Fragments arranged by size:
1712 1562 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5253 5285 5253
Size: 32 5261
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5293
XbaI T'CTAG_A
Cuts at: 2629 2629
Size: 5293
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5293
XmaI C'CCGG_G
Cuts at: 1870 2181 2618 1870
Size: 311 437 4545
Fragments arranged by size:
4545 437 311
XmnI GAAnn'nnTTC
Cuts at: 2396 3422 2396
Size: 1026 4267
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3101 276
Size: 53 83 186 936 1567 2468
Fragments arranged by size:
2468 1567 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI