Restriction map for
pFN21AE5232
(Circular) (Six-base) MAPSORT of: pFN21AE5232.seq Check: 8150 from: 1 to: 5458
pFN21AE5232 5458 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3268 278
Size: 53 83 186 936 1732 2468
Fragments arranged by size:
2468 1732 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2577 2801 1937
Size: 640 224 4594
Fragments arranged by size:
4594 640 224
Acc65I G'GTAC_C
Cuts at: 2779 2779
Size: 5458
AclI AA'CG_TT
Cuts at: 1563 3585 3958 1563
Size: 2022 373 3063
Fragments arranged by size:
3063 2022 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2212 2459 3521 4905 5173 1632
Size: 580 247 1062 1384 268 1917
Fragments arranged by size:
1917 1384 1062 580 268 247
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5261
AflIII A'CryG_T
Cuts at: 1203 4357 5334 1203
Size: 3154 977 1327
Fragments arranged by size:
3154 1327 977
AgeI A'CCGG_T
Cuts at: 4289 4289
Size: 5458
AhdI GACnn_n'nnGTC
Cuts at: 4187 4187
Size: 5458
AleI CACnn'nnGTG
Cuts at: 1166 2370 1166
Size: 1204 4254
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5458
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2576 2608 2576
Size: 32 5426
AlwNI CAG_nnn'CTG
Cuts at: 3201 4773 3201
Size: 1572 3886
ApaLI G'TGCA_C
Cuts at: 3515 4671 3515
Size: 1156 4302
ApoI r'AATT_y
Cuts at: 1493 2767 2993 3145 5254 1493
Size: 1274 226 152 2109 1697
Fragments arranged by size:
2109 1697 1274 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4012 160
Size: 872 2980 1606
Fragments arranged by size:
2980 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
AvaI C'yCGr_G
Cuts at: 1870 1943 2330 2783 1870
Size: 73 387 453 4545
Fragments arranged by size:
4545 453 387 73
BamHI G'GATC_C
Cuts at: 2788 3155 2788
Size: 367 5091
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2728 2779 4234 618
Size: 325 217 627 941 51 1455 1842
Fragments arranged by size:
1842 1455 941 627 325 217 51
BanII G_rGCy'C
Cuts at: 1391 2331 2676 2777 1391
Size: 940 345 101 4072
Fragments arranged by size:
4072 940 345 101
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4487
BbvCI CC'TCA_GC
Cuts at: 2655 2655
Size: 5458
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3649 3683 1903
Size: 34 1712 34 3678
Fragments arranged by size:
3678 1712 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3350 4560 3350
Size: 1210 4248
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5458
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4069 136
Size: 107 122 71 631 605 337 2060 1525
Fragments arranged by size:
2060 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5453 5453
Size: 5458
BlpI GC'TnA_GC
Cuts at: 2875 2875
Size: 5458
Bme1580I G_kGCm'C
Cuts at: 1790 3519 4675 1790
Size: 1729 1156 2573
Fragments arranged by size:
2573 1729 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5458
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3189 4147 442
Size: 416 53 83 399 24 1772 958 1753
Fragments arranged by size:
1772 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5458
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2722 2722
Size: 5458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4118 1239
Size: 2879 2579
Bpu10I CC'TnA_GC
Cuts at: 2655 2655
Size: 5458
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3583 4448 4746 3583
Size: 865 298 4295
Fragments arranged by size:
4295 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4121 882
Size: 617 2622 2219
Fragments arranged by size:
2622 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3249 493
Size: 711 2045 2702
Fragments arranged by size:
2702 2045 711
BsaBI GATnn'nnATC
Cuts at: 5452 5452
Size: 5458
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2469 3265 3647 4282
Size: 53 83 186 936 936 796 382 635
Cuts at: 4282 275
Size: 1451
Fragments arranged by size:
1451 936 936 796 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3891 4289 4563 4710 5121 3891
Size: 398 274 147 411 4228
Fragments arranged by size:
4228 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2576 2606 1178
Size: 30 165 30 1173 30 4030
Fragments arranged by size:
4030 1173 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2435 2438 2458 2522 2525 2597 2678 2681
Size: 1249 3 20 64 3 72 81 3
Cuts at: 2681 2684 2714 1186
Size: 3 30 3930
Fragments arranged by size:
3930 1249 81 72 64 30 20 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2103 4661 5157 2103
Size: 2558 496 2404
Fragments arranged by size:
2558 2404 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4885
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2574 2908 3669 4697 1540
Size: 275 186 573 334 761 1028 2301
Fragments arranged by size:
2301 1028 761 573 334 275 186
BsiHKAI G_wGCw'C
Cuts at: 2097 2676 3519 3604 4675 2097
Size: 579 843 85 1071 2880
Fragments arranged by size:
2880 1071 843 579 85
BsmI GAATG_Cn'
Cuts at: 2969 3062 2969
Size: 93 5365
BsmBI CGTCTCn'nnnn_
Cuts at: 2138 2304 2138
Size: 166 5292
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2331 2676 2777 3519 3604 4675
Size: 399 307 234 345 101 742 85 1071
Cuts at: 4675 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 345 307 234 101
85
BspEI T'CCGG_A
Cuts at: 5121 5121
Size: 5458
BspHI T'CATG_A
Cuts at: 3347 5077 3347
Size: 1730 3728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2813 844
Size: 1969 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2037 3345 1394
Size: 429 214 1308 3507
Fragments arranged by size:
3507 1308 429 214
BsrDI GCAATG_nn'
Cuts at: 65 1248 3953 4127 65
Size: 1183 2705 174 1396
Fragments arranged by size:
2705 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2080 4102 4289 1700
Size: 110 270 2022 187 2869
Fragments arranged by size:
2869 2022 270 187 110
BsrGI T'GTAC_A
Cuts at: 96 2028 96
Size: 1932 3526
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5458
BssSI C'ACGA_G
Cuts at: 2141 2597 3518 4530 2141
Size: 456 921 1012 3069
Fragments arranged by size:
3069 1012 921 456
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5458
BstBI TT'CG_AA
Cuts at: 4262 4262
Size: 5458
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5458
BstXI CCAn_nnnn'nTGG
Cuts at: 2372 2372
Size: 5458
BstYI r'GATC_y
Cuts at: 1972 2788 3155 3540 3557 5009 5095 5107 5453
Size: 816 367 385 17 1452 86 12 346
Cuts at: 5453 1972
Size: 1977
Fragments arranged by size:
1977 1452 816 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2262 513
Size: 552 1197 3709
Fragments arranged by size:
3709 1197 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4719
Fragments arranged by size:
4719 683 56
BtsI GCAGTG_nn'
Cuts at: 2974 3768 3788 2974
Size: 794 20 4644
Fragments arranged by size:
4644 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5458
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5423
DraI TTT'AAA
Cuts at: 2762 2762
Size: 5458
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4465 809
Size: 507 3149 1802
Fragments arranged by size:
3149 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2078 2321 2571
Size: 54 1425 50 275 18 248 243 250
Cuts at: 2571 2905 3794 5423 8
Size: 334 889 1629 43
Fragments arranged by size:
1629 1425 889 334 275 250 248 243
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2571 2905 1537
Size: 275 759 334 4090
Fragments arranged by size:
4090 759 334 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2435 3388 1353
Size: 1082 953 3423
Fragments arranged by size:
3423 1082 953
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2531 2537 2549 2754 4045 4419
Size: 1064 138 942 6 12 205 1291 374
Cuts at: 4419 4565 387
Size: 146 1280
Fragments arranged by size:
1291 1280 1064 942 374 205 146 138
12 6
EcoICRI GAG'CTC
Cuts at: 2674 2674
Size: 5458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2212 2459 3521 4118 4905 5173 1239
Size: 393 580 247 1062 597 787 268 1524
Fragments arranged by size:
1524 1062 787 597 580 393 268 247
EcoO109I rG'GnC_Cy
Cuts at: 2041 2136 2455 2041
Size: 95 319 5044
Fragments arranged by size:
5044 319 95
EcoRI G'AATT_C
Cuts at: 2767 3145 2767
Size: 378 5080
FspI TGC'GCA
Cuts at: 3964 3964
Size: 5458
HaeII r_GCGC'y
Cuts at: 1678 2622 4605 1678
Size: 944 1983 2531
Fragments arranged by size:
2531 1983 944
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4099 4131 4173 4205 1373
Size: 32 135 32 2527 32 42 32 2626
Fragments arranged by size:
2626 2527 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2578 2802 3048 669
Size: 988 281 640 224 246 3079
Fragments arranged by size:
3079 988 640 281 246 224
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5458
HpaI GTT'AAC
Cuts at: 3048 3048
Size: 5458
KpnI G_GTAC'C
Cuts at: 2783 2783
Size: 5458
MfeI C'AATT_G
Cuts at: 3057 3057
Size: 5458
MluI A'CGCG_T
Cuts at: 5334 5334
Size: 5458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2301 4572 4756 1305
Size: 996 2271 184 2007
Fragments arranged by size:
2271 2007 996 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3636
Fragments arranged by size:
3636 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2024 2370 3416 3775 3934 518
Size: 648 858 346 1046 359 159 2042
Fragments arranged by size:
2042 1046 858 648 359 346 159
MspA1I CmG'CkG
Cuts at: 1606 2307 2437 2874 3551 4699 4944 1606
Size: 701 130 437 677 1148 245 2120
Fragments arranged by size:
2120 1148 701 677 437 245 130
NaeI GCC'GGC
Cuts at: 2082 2082
Size: 5458
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4906
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5458
NgoMIV G'CCGG_C
Cuts at: 2080 2080
Size: 5458
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5458
NotI GC'GGCC_GC
Cuts at: 2905 2905
Size: 5458
NruI TCG'CGA
Cuts at: 2748 2748
Size: 5458
NspI r_CATG'y
Cuts at: 1132 1229 2023 2723 2816 4361 1132
Size: 97 794 700 93 1545 2229
Fragments arranged by size:
2229 1545 794 700 97 93
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5458
PciI A'CATG_T
Cuts at: 4357 4357
Size: 5458
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5458
PfoI T'CCnGG_A
Cuts at: 2676 2676
Size: 5458
PmeI GTTT'AAAC
Cuts at: 2762 2762
Size: 5458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2576 2608 3527 3559 5040 5072 2576
Size: 32 919 32 1481 32 2962
Fragments arranged by size:
2962 1481 919 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2041 2136 2455 2041
Size: 95 319 5044
Fragments arranged by size:
5044 319 95
PshAI GACnn'nnGTC
Cuts at: 1613 2573 1613
Size: 960 4498
PsiI TTA'TAA
Cuts at: 3028 3028
Size: 5458
PstI C_TGCA'G
Cuts at: 830 2810 830
Size: 1980 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5458
SacI G_AGCT'C
Cuts at: 2676 2676
Size: 5458
SalI G'TCGA_C
Cuts at: 1936 2576 2800 1936
Size: 640 224 4594
Fragments arranged by size:
4594 640 224
SanDI GG'GwC_CC
Cuts at: 2136 2455 2136
Size: 319 5139
SbfI CC_TGCA'GG
Cuts at: 2810 2810
Size: 5458
ScaI AGT'ACT
Cuts at: 3706 5418 3706
Size: 1712 3746
SexAI A'CCwGG_T
Cuts at: 2043 2118 2043
Size: 75 5383
SfcI C'TryA_G
Cuts at: 826 1045 2806 3941 4622 4813 826
Size: 219 1761 1135 681 191 1471
Fragments arranged by size:
1761 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5458
SmaI CCC'GGG
Cuts at: 1872 2785 1872
Size: 913 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3562 4463 4725 820
Size: 197 926 1619 901 262 1553
Fragments arranged by size:
1619 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3249 493
Size: 2756 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5458
SphI G_CATG'C
Cuts at: 1132 2023 2723 2816 1132
Size: 891 700 93 3774
Fragments arranged by size:
3774 891 700 93
SspI AAT'ATT
Cuts at: 5 52 3382 5
Size: 47 3330 2081
Fragments arranged by size:
3330 2081 47
StuI AGG'CCT
Cuts at: 1517 2273 2705 1517
Size: 756 432 4270
Fragments arranged by size:
4270 756 432
StyI C'CwwG_G
Cuts at: 513 1065 2318 2484 2897 513
Size: 552 1253 166 413 3074
Fragments arranged by size:
3074 1253 552 413 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3485 3502 3655 3840 4285 325
Size: 1255 1905 17 153 185 445 1498
Fragments arranged by size:
1905 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2028 3704 5416
Size: 274 80 33 51 1443 51 1676 1712
Cuts at: 5416 96
Size: 138
Fragments arranged by size:
1712 1676 1443 274 138 80 51 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5418 5450 5418
Size: 32 5426
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5458
XbaI T'CTAG_A
Cuts at: 2794 2794
Size: 5458
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5458
XmaI C'CCGG_G
Cuts at: 1870 2783 1870
Size: 913 4545
XmnI GAAnn'nnTTC
Cuts at: 3587 3587
Size: 5458
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3266 276
Size: 53 83 186 936 1732 2468
Fragments arranged by size:
2468 1732 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI
FspAI KasI NarI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacII SapI SfiI SfoI SrfI
SwaI XcmI