Restriction map for
pFN21AE5231
(Circular) (Six-base) MAPSORT of: pFN21AE5231.seq Check: 2411 from: 1 to: 5380
pFN21AE5231 5380 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2159 3190 278
Size: 53 83 186 936 623 1031 2468
Fragments arranged by size:
2468 1031 936 623 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2229 2723 1937
Size: 292 494 4594
Fragments arranged by size:
4594 494 292
Acc65I G'GTAC_C
Cuts at: 2701 2701
Size: 5380
AclI AA'CG_TT
Cuts at: 1563 3507 3880 1563
Size: 1944 373 3063
Fragments arranged by size:
3063 1944 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3443 4827 5095 1632
Size: 1811 1384 268 1917
Fragments arranged by size:
1917 1811 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5183
AflIII A'CryG_T
Cuts at: 1203 4279 5256 1203
Size: 3076 977 1327
Fragments arranged by size:
3076 1327 977
AgeI A'CCGG_T
Cuts at: 4211 4211
Size: 5380
AhdI GACnn_n'nnGTC
Cuts at: 2317 4109 2317
Size: 1792 3588
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5380
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5380
AlwNI CAG_nnn'CTG
Cuts at: 2464 3123 4695 2464
Size: 659 1572 3149
Fragments arranged by size:
3149 1572 659
ApaLI G'TGCA_C
Cuts at: 3437 4593 3437
Size: 1156 4224
ApoI r'AATT_y
Cuts at: 1493 2255 2689 2915 3067 5176 1493
Size: 762 434 226 152 2109 1697
Fragments arranged by size:
2109 1697 762 434 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3934 160
Size: 872 2902 1606
Fragments arranged by size:
2902 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
AvaI C'yCGr_G
Cuts at: 1870 1943 2142 2399 2659 2705 1870
Size: 73 199 257 260 46 4545
Fragments arranged by size:
4545 260 257 199 73 46
BamHI G'GATC_C
Cuts at: 2710 3077 2710
Size: 367 5013
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2261 2593 2701 4156 618
Size: 325 217 627 474 332 108 1455 1842
Fragments arranged by size:
1842 1455 627 474 332 325 217 108
BanII G_rGCy'C
Cuts at: 1391 2016 2400 2454 2472 2699 1391
Size: 625 384 54 18 227 4072
Fragments arranged by size:
4072 625 384 227 54 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4409
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3571 3605 1903
Size: 34 1634 34 3678
Fragments arranged by size:
3678 1634 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2038 3272 4482 2038
Size: 1234 1210 2936
Fragments arranged by size:
2936 1234 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5380
BfrBI ATG'CAT
Cuts at: 2542 2542
Size: 5380
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3991 136
Size: 107 122 71 631 605 2319 1525
Fragments arranged by size:
2319 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5375 5375
Size: 5380
BlpI GC'TnA_GC
Cuts at: 2453 2797 2453
Size: 344 5036
Bme1580I G_kGCm'C
Cuts at: 1790 2564 3441 4597 1790
Size: 774 877 1156 2573
Fragments arranged by size:
2573 1156 877 774
BmgBI CAC'GTC
Cuts at: 1318 2314 1318
Size: 996 4384
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3111 4069 442
Size: 416 53 83 399 24 1694 958 1753
Fragments arranged by size:
1753 1694 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5380
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2665 2665
Size: 5380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2402 2686 4040 1239
Size: 917 246 284 1354 2579
Fragments arranged by size:
2579 1354 917 284 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3505 4370 4668 3505
Size: 865 298 4217
Fragments arranged by size:
4217 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2411 4043 882
Size: 617 912 1632 2219
Fragments arranged by size:
2219 1632 912 617
BsaAI yAC'GTr
Cuts at: 493 1204 2106 2227 3171 493
Size: 711 902 121 944 2702
Fragments arranged by size:
2702 944 902 711 121
BsaBI GATnn'nnATC
Cuts at: 5374 5374
Size: 5380
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2156 2433 2550 2580
Size: 53 83 186 936 623 277 117 30
Cuts at: 2580 3187 3569 4204 275
Size: 607 382 635 1451
Fragments arranged by size:
1451 936 635 623 607 382 277 186
117 83 53 30
BsaWI w'CCGG_w
Cuts at: 2505 3813 4211 4485 4632 5043 2505
Size: 1308 398 274 147 411 2842
Fragments arranged by size:
2842 1308 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5155
Fragments arranged by size:
5155 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2462 1186
Size: 1276 4104
BseYI C'CCAG_C
Cuts at: 2149 4583 5079 2149
Size: 2434 496 2450
Fragments arranged by size:
2450 2434 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2507 1163
Size: 573 771 4036
Fragments arranged by size:
4036 771 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2830 3591 4619 1540
Size: 275 186 829 761 1028 2301
Fragments arranged by size:
2301 1028 829 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2016 2400 2454 3441 3526 4597 2016
Size: 384 54 987 85 1071 2799
Fragments arranged by size:
2799 1071 987 384 85 54
BsmI GAATG_Cn'
Cuts at: 2297 2891 2984 2297
Size: 594 93 4693
Fragments arranged by size:
4693 594 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2400 2454 2472 2564 2699 3441
Size: 399 226 384 54 18 92 135 742
Cuts at: 3441 3526 4597 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 384 226 135 92
85 54 18
BspEI T'CCGG_A
Cuts at: 5043 5043
Size: 5380
BspHI T'CATG_A
Cuts at: 3269 4999 3269
Size: 1730 3650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2479 2735 844
Size: 1635 256 3489
Fragments arranged by size:
3489 1635 256
BsrBI CCG'CTC
Cuts at: 1394 1823 2127 3267 1394
Size: 429 304 1140 3507
Fragments arranged by size:
3507 1140 429 304
BsrDI GCAATG_nn'
Cuts at: 65 1248 3875 4049 65
Size: 1183 2627 174 1396
Fragments arranged by size:
2627 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4024 4211 1700
Size: 110 2214 187 2869
Fragments arranged by size:
2869 2214 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5380
BssHII G'CGCG_C
Cuts at: 1925 2135 2480 1925
Size: 210 345 4825
Fragments arranged by size:
4825 345 210
BssSI C'ACGA_G
Cuts at: 3440 4452 3440
Size: 1012 4368
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5380
BstBI TT'CG_AA
Cuts at: 4184 4184
Size: 5380
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5380
BstYI r'GATC_y
Cuts at: 1972 2710 3077 3462 3479 4931 5017 5029 5375
Size: 738 367 385 17 1452 86 12 346
Cuts at: 5375 1972
Size: 1977
Fragments arranged by size:
1977 1452 738 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3889
Fragments arranged by size:
3889 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4641
Fragments arranged by size:
4641 683 56
BtsI GCAGTG_nn'
Cuts at: 2197 2896 3690 3710 2197
Size: 699 794 20 3867
Fragments arranged by size:
3867 794 699 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5380
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5345
DraI TTT'AAA
Cuts at: 2684 2684
Size: 5380
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4387 809
Size: 507 3071 1802
Fragments arranged by size:
3071 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2194 2827 3716
Size: 54 1425 50 275 18 364 633 889
Cuts at: 3716 5345 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 633 364 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2827 1537
Size: 275 1015 4090
Fragments arranged by size:
4090 1015 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2004 2414 3310 1353
Size: 651 410 896 3423
Fragments arranged by size:
3423 896 651 410
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2082 2088 2097 3967 4341 4487
Size: 1064 138 493 6 9 1870 374 146
Cuts at: 4487 387
Size: 1280
Fragments arranged by size:
1870 1280 1064 493 374 146 138 9
6
EcoICRI GAG'CTC
Cuts at: 2014 2398 2452 2014
Size: 384 54 4942
Fragments arranged by size:
4942 384 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2402 2686 3443 4040 4827 5095
Size: 393 524 246 284 757 597 787 268
Cuts at: 5095 1239
Size: 1524
Fragments arranged by size:
1524 787 757 597 524 393 284 268
246
EcoRI G'AATT_C
Cuts at: 2255 2689 3067 2255
Size: 434 378 4568
Fragments arranged by size:
4568 434 378
FspI TGC'GCA
Cuts at: 3886 3886
Size: 5380
HaeII r_GCGC'y
Cuts at: 1678 2464 4527 1678
Size: 786 2063 2531
Fragments arranged by size:
2531 2063 786
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2303 2335 4021 4053 4095
Size: 32 135 32 731 32 1686 32 42
Cuts at: 4095 4127 1373
Size: 32 2626
Fragments arranged by size:
2626 1686 731 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2605 2724 2970 669
Size: 988 281 667 119 246 3079
Fragments arranged by size:
3079 988 667 281 246 119
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5380
HpaI GTT'AAC
Cuts at: 2970 2970
Size: 5380
KpnI G_GTAC'C
Cuts at: 2705 2705
Size: 5380
MfeI C'AATT_G
Cuts at: 2979 2979
Size: 5380
MluI A'CGCG_T
Cuts at: 5256 5256
Size: 5380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2139 2301 2656 4494 4678 1305
Size: 834 162 355 1838 184 2007
Fragments arranged by size:
2007 1838 834 355 184 162
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3558
Fragments arranged by size:
3558 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3338 3697 3856 518
Size: 648 2172 359 159 2042
Fragments arranged by size:
2172 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2389 2796 3473 4621 4866 1606
Size: 783 407 677 1148 245 2120
Fragments arranged by size:
2120 1148 783 677 407 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3889
Fragments arranged by size:
3889 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5380
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5380
NotI GC'GGCC_GC
Cuts at: 2827 2827
Size: 5380
NsiI A_TGCA'T
Cuts at: 2544 2544
Size: 5380
NspI r_CATG'y
Cuts at: 1132 1229 2738 4283 1132
Size: 97 1509 1545 2229
Fragments arranged by size:
2229 1545 1509 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5380
PciI A'CATG_T
Cuts at: 4279 4279
Size: 5380
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2464 2638 1108
Size: 1356 174 3850
Fragments arranged by size:
3850 1356 174
PfoI T'CCnGG_A
Cuts at: 2172 2172
Size: 5380
PmeI GTTT'AAAC
Cuts at: 2684 2684
Size: 5380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 4962 4994 3449
Size: 32 1481 32 3835
Fragments arranged by size:
3835 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5380
PsiI TTA'TAA
Cuts at: 2950 2950
Size: 5380
PspXI vC'TCGA_Gb
Cuts at: 2399 2399
Size: 5380
PstI C_TGCA'G
Cuts at: 830 2732 830
Size: 1902 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5380
PvuII CAG'CTG
Cuts at: 2389 2389
Size: 5380
SacI G_AGCT'C
Cuts at: 2016 2400 2454 2016
Size: 384 54 4942
Fragments arranged by size:
4942 384 54
SalI G'TCGA_C
Cuts at: 1936 2722 1936
Size: 786 4594
SapI GCTCTTCn'nnn_
Cuts at: 2004 2004
Size: 5380
SbfI CC_TGCA'GG
Cuts at: 2732 2732
Size: 5380
ScaI AGT'ACT
Cuts at: 3628 5340 3628
Size: 1712 3668
SfcI C'TryA_G
Cuts at: 826 1045 2728 3863 4544 4735 826
Size: 219 1683 1135 681 191 1471
Fragments arranged by size:
1683 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5380
SmaI CCC'GGG
Cuts at: 1872 2144 2661 2707 1872
Size: 272 517 46 4545
Fragments arranged by size:
4545 517 272 46
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2399 3484 4385 4647 820
Size: 197 926 456 1085 901 262 1553
Fragments arranged by size:
1553 1085 926 901 456 262 197
SnaBI TAC'GTA
Cuts at: 493 2106 3171 493
Size: 1613 1065 2702
Fragments arranged by size:
2702 1613 1065
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5380
SphI G_CATG'C
Cuts at: 1132 2738 1132
Size: 1606 3774
SrfI GCCC'GGGC
Cuts at: 2661 2661
Size: 5380
SspI AAT'ATT
Cuts at: 5 52 3304 5
Size: 47 3252 2081
Fragments arranged by size:
3252 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5380
StyI C'CwwG_G
Cuts at: 513 1065 2004 2819 513
Size: 552 939 815 3074
Fragments arranged by size:
3074 939 815 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3407 3424 3577 3762 4207 325
Size: 1255 1827 17 153 185 445 1498
Fragments arranged by size:
1827 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3626 5338 96
Size: 274 80 33 51 1443 1649 1712 138
Fragments arranged by size:
1712 1649 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5340 5372 5340
Size: 32 5348
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5380
XbaI T'CTAG_A
Cuts at: 2716 2716
Size: 5380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2377 2377
Size: 5380
XhoI C'TCGA_G
Cuts at: 1943 2399 1943
Size: 456 4924
XmaI C'CCGG_G
Cuts at: 1870 2142 2659 2705 1870
Size: 272 517 46 4545
Fragments arranged by size:
4545 517 272 46
XmnI GAAnn'nnTTC
Cuts at: 2255 3509 2255
Size: 1254 4126
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2157 3188 276
Size: 53 83 186 936 623 1031 2468
Fragments arranged by size:
2468 1031 936 623 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SwaI