Restriction map for
pFN21AE5224
(Circular) (Six-base) MAPSORT of: pFN21AE5224.seq Check: 7414 from: 1 to: 5287
pFN21AE5224 5287 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3097 278
Size: 53 83 186 936 1561 2468
Fragments arranged by size:
2468 1561 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2630 1937
Size: 693 4594
Acc65I G'GTAC_C
Cuts at: 2608 2608
Size: 5287
AclI AA'CG_TT
Cuts at: 1563 3414 3787 1563
Size: 1851 373 3063
Fragments arranged by size:
3063 1851 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3350 4734 5002 1632
Size: 1718 1384 268 1917
Fragments arranged by size:
1917 1718 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5090
AflIII A'CryG_T
Cuts at: 1203 4186 5163 1203
Size: 2983 977 1327
Fragments arranged by size:
2983 1327 977
AgeI A'CCGG_T
Cuts at: 4118 4118
Size: 5287
AhdI GACnn_n'nnGTC
Cuts at: 4016 4016
Size: 5287
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5287
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2582 1184
Size: 1398 3889
AlwNI CAG_nnn'CTG
Cuts at: 3030 4602 3030
Size: 1572 3715
ApaLI G'TGCA_C
Cuts at: 2405 3344 4500 2405
Size: 939 1156 3192
Fragments arranged by size:
3192 1156 939
ApoI r'AATT_y
Cuts at: 1493 2374 2596 2822 2974 5083 1493
Size: 881 222 226 152 2109 1697
Fragments arranged by size:
2109 1697 881 226 222 152
AseI AT'TA_AT
Cuts at: 160 1032 3841 160
Size: 872 2809 1606
Fragments arranged by size:
2809 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5287
AvaI C'yCGr_G
Cuts at: 1870 1943 2041 2612 1870
Size: 73 98 571 4545
Fragments arranged by size:
4545 571 98 73
BamHI G'GATC_C
Cuts at: 2617 2984 2617
Size: 367 4920
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2608 4063 618
Size: 325 217 627 821 1455 1842
Fragments arranged by size:
1842 1455 821 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2478 2606 1391
Size: 1087 128 4072
Fragments arranged by size:
4072 1087 128
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2508 928
Size: 971 609 3707
Fragments arranged by size:
3707 971 609
BbvCI CC'TCA_GC
Cuts at: 2081 2081
Size: 5287
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3478 3512 1903
Size: 34 50 34 1457 34 3678
Fragments arranged by size:
3678 1457 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2498 3179 4389 2498
Size: 681 1210 3396
Fragments arranged by size:
3396 1210 681
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5287
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3898 136
Size: 107 122 71 631 605 2226 1525
Fragments arranged by size:
2226 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2368 5282 2368
Size: 2914 2373
BlpI GC'TnA_GC
Cuts at: 2704 2704
Size: 5287
Bme1580I G_kGCm'C
Cuts at: 1790 2409 3348 4504 1790
Size: 619 939 1156 2573
Fragments arranged by size:
2573 1156 939 619
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5287
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3018 3976 442
Size: 416 53 83 399 24 1601 958 1753
Fragments arranged by size:
1753 1601 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5287
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3947 1239
Size: 2708 2579
Bpu10I CC'TnA_GC
Cuts at: 2081 2081
Size: 5287
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3412 4277 4575 3412
Size: 865 298 4124
Fragments arranged by size:
4124 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2384 3950 882
Size: 617 885 1566 2219
Fragments arranged by size:
2219 1566 885 617
BsaAI yAC'GTr
Cuts at: 493 1204 2128 2410 3078 493
Size: 711 924 282 668 2702
Fragments arranged by size:
2702 924 711 668 282
BsaBI GATnn'nnATC
Cuts at: 5281 5281
Size: 5287
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3094 3476 4111 275
Size: 53 83 186 936 1561 382 635 1451
Fragments arranged by size:
1561 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2055 3720 4118 4392 4539 4950 2055
Size: 1665 398 274 147 411 2392
Fragments arranged by size:
2392 1665 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5062
Fragments arranged by size:
5062 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2456 2558 1186
Size: 1270 102 3915
Fragments arranged by size:
3915 1270 102
BseYI C'CCAG_C
Cuts at: 4490 4986 4490
Size: 496 4791
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2552 1163
Size: 573 816 3898
Fragments arranged by size:
3898 816 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2737 3498 4526 1540
Size: 275 186 736 761 1028 2301
Fragments arranged by size:
2301 1028 761 736 275 186
BsiHKAI G_wGCw'C
Cuts at: 2042 2409 2478 3348 3433 4504 2042
Size: 367 69 870 85 1071 2825
Fragments arranged by size:
2825 1071 870 367 85 69
BsmI GAATG_Cn'
Cuts at: 2798 2891 2798
Size: 93 5194
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2409 2478 2606 3348 3433 4504
Size: 399 252 367 69 128 742 85 1071
Cuts at: 4504 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 367 252 128 85
69
BspEI T'CCGG_A
Cuts at: 4950 4950
Size: 5287
BspHI T'CATG_A
Cuts at: 3176 4906 3176
Size: 1730 3557
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2402 2642 844
Size: 1558 240 3489
Fragments arranged by size:
3489 1558 240
BsrBI CCG'CTC
Cuts at: 1394 1823 3174 1394
Size: 429 1351 3507
Fragments arranged by size:
3507 1351 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3782 3956 65
Size: 1183 2534 174 1396
Fragments arranged by size:
2534 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3931 4118 1700
Size: 110 2121 187 2869
Fragments arranged by size:
2869 2121 187 110
BsrGI T'GTAC_A
Cuts at: 96 2196 96
Size: 2100 3187
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5287
BssSI C'ACGA_G
Cuts at: 2438 3347 4359 2438
Size: 909 1012 3366
Fragments arranged by size:
3366 1012 909
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5287
BstBI TT'CG_AA
Cuts at: 4091 4091
Size: 5287
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5287
BstYI r'GATC_y
Cuts at: 1972 2368 2617 2984 3369 3386 4838 4924 4936
Size: 396 249 367 385 17 1452 86 12
Cuts at: 4936 5282 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 396 385 367 346 249 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4735
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4548
Fragments arranged by size:
4548 683 56
BtsI GCAGTG_nn'
Cuts at: 2803 3597 3617 2803
Size: 794 20 4473
Fragments arranged by size:
4473 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5287
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5252
DraI TTT'AAA
Cuts at: 2591 2591
Size: 5287
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4294 809
Size: 507 2978 1802
Fragments arranged by size:
2978 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2285 2554 2734
Size: 54 1425 50 275 18 455 269 180
Cuts at: 2734 3623 5252 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 455 275 269 180 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2734 1537
Size: 275 922 4090
Fragments arranged by size:
4090 922 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2334 2543 3217 1353
Size: 981 209 674 3423
Fragments arranged by size:
3423 981 674 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3874 4248 4394 387
Size: 1064 138 2285 374 146 1280
Fragments arranged by size:
2285 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2476 2476
Size: 5287
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3350 3947 4734 5002 1239
Size: 393 1718 597 787 268 1524
Fragments arranged by size:
1718 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2374 2596 2974 2374
Size: 222 378 4687
Fragments arranged by size:
4687 378 222
FspI TGC'GCA
Cuts at: 3793 3793
Size: 5287
HaeII r_GCGC'y
Cuts at: 1678 4434 1678
Size: 2756 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2085 2117 3928 3960 4002
Size: 32 135 32 513 32 1811 32 42
Cuts at: 4002 4034 1373
Size: 32 2626
Fragments arranged by size:
2626 1811 513 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2631 2877 669
Size: 988 281 693 246 3079
Fragments arranged by size:
3079 988 693 281 246
HindIII A'AGCT_T
Cuts at: 748 2132 748
Size: 1384 3903
HpaI GTT'AAC
Cuts at: 2877 2877
Size: 5287
KpnI G_GTAC'C
Cuts at: 2612 2612
Size: 5287
MfeI C'AATT_G
Cuts at: 2886 2886
Size: 5287
MluI A'CGCG_T
Cuts at: 5163 5163
Size: 5287
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4401 4585 1305
Size: 3096 184 2007
Fragments arranged by size:
3096 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3465
Fragments arranged by size:
3465 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3245 3604 3763 518
Size: 648 2079 359 159 2042
Fragments arranged by size:
2079 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2020 2703 3380 4528 4773 1606
Size: 414 683 677 1148 245 2120
Fragments arranged by size:
2120 1148 683 677 414 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4735
NdeI CA'TA_TG
Cuts at: 387 2185 2253 387
Size: 1798 68 3421
Fragments arranged by size:
3421 1798 68
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5287
NotI GC'GGCC_GC
Cuts at: 2734 2734
Size: 5287
NspI r_CATG'y
Cuts at: 1132 1229 2645 4190 1132
Size: 97 1416 1545 2229
Fragments arranged by size:
2229 1545 1416 97
PasI CC'CwG_GG
Cuts at: 1348 2452 1348
Size: 1104 4183
PciI A'CATG_T
Cuts at: 4186 4186
Size: 5287
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2362 1108
Size: 1254 4033
PfoI T'CCnGG_A
Cuts at: 2137 2427 2137
Size: 290 4997
PmeI GTTT'AAAC
Cuts at: 2591 2591
Size: 5287
PmlI CAC'GTG
Cuts at: 2128 2410 2128
Size: 282 5005
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2107 2139 3356 3388 4869 4901 2107
Size: 32 1217 32 1481 32 2493
Fragments arranged by size:
2493 1481 1217 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5287
PsiI TTA'TAA
Cuts at: 2857 2857
Size: 5287
PspXI vC'TCGA_Gb
Cuts at: 2041 2041
Size: 5287
PstI C_TGCA'G
Cuts at: 830 2535 2639 830
Size: 1705 104 3478
Fragments arranged by size:
3478 1705 104
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5287
SacI G_AGCT'C
Cuts at: 2478 2478
Size: 5287
SalI G'TCGA_C
Cuts at: 1936 2629 1936
Size: 693 4594
SbfI CC_TGCA'GG
Cuts at: 2639 2639
Size: 5287
ScaI AGT'ACT
Cuts at: 3535 5247 3535
Size: 1712 3575
SexAI A'CCwGG_T
Cuts at: 2355 2355
Size: 5287
SfcI C'TryA_G
Cuts at: 826 1045 2531 2635 3770 4451 4642 826
Size: 219 1486 104 1135 681 191 1471
Fragments arranged by size:
1486 1471 1135 681 219 191 104
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5287
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5287
SmaI CCC'GGG
Cuts at: 1872 2614 1872
Size: 742 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2041 3391 4292 4554 820
Size: 197 926 98 1350 901 262 1553
Fragments arranged by size:
1553 1350 926 901 262 197 98
SnaBI TAC'GTA
Cuts at: 493 3078 493
Size: 2585 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5287
SphI G_CATG'C
Cuts at: 1132 2645 1132
Size: 1513 3774
SspI AAT'ATT
Cuts at: 5 52 3211 5
Size: 47 3159 2081
Fragments arranged by size:
3159 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5287
StyI C'CwwG_G
Cuts at: 513 1065 2726 513
Size: 552 1661 3074
Fragments arranged by size:
3074 1661 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3314 3331 3484 3669 4114 325
Size: 1255 1734 17 153 185 445 1498
Fragments arranged by size:
1734 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2196 3533 5245
Size: 274 80 33 51 1443 219 1337 1712
Cuts at: 5245 96
Size: 138
Fragments arranged by size:
1712 1443 1337 274 219 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5247 5279 5247
Size: 32 5255
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5287
XbaI T'CTAG_A
Cuts at: 2623 2623
Size: 5287
XhoI C'TCGA_G
Cuts at: 1943 2041 1943
Size: 98 5189
XmaI C'CCGG_G
Cuts at: 1870 2612 1870
Size: 742 4545
XmnI GAAnn'nnTTC
Cuts at: 3416 3416
Size: 5287
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3095 276
Size: 53 83 186 936 1561 2468
Fragments arranged by size:
2468 1561 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PstI
PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PsrI
PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI