Restriction map for
pFN21AE5222
(Circular) (Six-base) MAPSORT of: pFN21AE5222.seq Check: 6937 from: 1 to: 5473
pFN21AE5222 5473 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3283 278
Size: 53 83 186 936 1747 2468
Fragments arranged by size:
2468 1747 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2816 1937
Size: 879 4594
Acc65I G'GTAC_C
Cuts at: 2794 2794
Size: 5473
AclI AA'CG_TT
Cuts at: 1563 3600 3973 1563
Size: 2037 373 3063
Fragments arranged by size:
3063 2037 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2090 3536 4920 5188 1632
Size: 458 1446 1384 268 1917
Fragments arranged by size:
1917 1446 1384 458 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5276
AflIII A'CryG_T
Cuts at: 1203 4372 5349 1203
Size: 3169 977 1327
Fragments arranged by size:
3169 1327 977
AgeI A'CCGG_T
Cuts at: 4304 4304
Size: 5473
AhdI GACnn_n'nnGTC
Cuts at: 4202 4202
Size: 5473
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5473
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5473
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2240 2272 2240
Size: 32 5441
AlwNI CAG_nnn'CTG
Cuts at: 2065 2068 2686 3216 4788 2065
Size: 3 618 530 1572 2750
Fragments arranged by size:
2750 1572 618 530 3
ApaLI G'TGCA_C
Cuts at: 3530 4686 3530
Size: 1156 4317
ApoI r'AATT_y
Cuts at: 1493 2782 3008 3160 5269 1493
Size: 1289 226 152 2109 1697
Fragments arranged by size:
2109 1697 1289 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4027 160
Size: 872 2995 1606
Fragments arranged by size:
2995 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5473
AvaI C'yCGr_G
Cuts at: 1870 1943 2373 2798 1870
Size: 73 430 425 4545
Fragments arranged by size:
4545 430 425 73
BamHI G'GATC_C
Cuts at: 2803 3170 2803
Size: 367 5106
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2794 4249 618
Size: 325 217 627 1007 1455 1842
Fragments arranged by size:
1842 1455 1007 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2016 2625 2792 1391
Size: 625 609 167 4072
Fragments arranged by size:
4072 625 609 167
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2078 2320 2732 928
Size: 971 179 242 412 3669
Fragments arranged by size:
3669 971 412 242 179
BbvCI CC'TCA_GC
Cuts at: 2666 2666
Size: 5473
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3664 3698 1903
Size: 34 1727 34 3678
Fragments arranged by size:
3678 1727 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3365 4575 3365
Size: 1210 4263
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5473
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4084 136
Size: 107 122 71 631 605 2412 1525
Fragments arranged by size:
2412 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5468 5468
Size: 5473
BlpI GC'TnA_GC
Cuts at: 2890 2890
Size: 5473
Bme1580I G_kGCm'C
Cuts at: 1790 3534 4690 1790
Size: 1744 1156 2573
Fragments arranged by size:
2573 1744 1156
BmgBI CAC'GTC
Cuts at: 1318 2336 1318
Size: 1018 4455
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2010 3204 4162
Size: 416 53 83 399 24 593 1194 958
Cuts at: 4162 442
Size: 1753
Fragments arranged by size:
1753 1194 958 593 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2612 2714 4133 1239
Size: 1373 102 1419 2579
Fragments arranged by size:
2579 1419 1373 102
Bpu10I CC'TnA_GC
Cuts at: 2666 2666
Size: 5473
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3598 4463 4761 3598
Size: 865 298 4310
Fragments arranged by size:
4310 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2048 4136 882
Size: 617 549 2088 2219
Fragments arranged by size:
2219 2088 617 549
BsaAI yAC'GTr
Cuts at: 493 1204 3264 493
Size: 711 2060 2702
Fragments arranged by size:
2702 2060 711
BsaBI GATnn'nnATC
Cuts at: 5467 5467
Size: 5473
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3280 3662 4297 275
Size: 53 83 186 936 1747 382 635 1451
Fragments arranged by size:
1747 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3906 4304 4578 4725 5136 3906
Size: 398 274 147 411 4243
Fragments arranged by size:
4243 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2125 2155 2325 2355 1178
Size: 30 165 30 722 30 170 30 4296
Fragments arranged by size:
4296 722 170 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2146 2333 2555 2558 1186
Size: 853 107 187 222 3 4101
Fragments arranged by size:
4101 853 222 187 107 3
BseYI C'CCAG_C
Cuts at: 2058 2406 2517 2679 4676 5172 2058
Size: 348 111 162 1997 496 2359
Fragments arranged by size:
2359 1997 496 348 162 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2555 1163
Size: 573 819 4081
Fragments arranged by size:
4081 819 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2718 2923 3684 4712 1540
Size: 275 186 717 205 761 1028 2301
Fragments arranged by size:
2301 1028 761 717 275 205 186
BsiHKAI G_wGCw'C
Cuts at: 2392 2625 3534 3619 4690 2392
Size: 233 909 85 1071 3175
Fragments arranged by size:
3175 1071 909 233 85
BsmI GAATG_Cn'
Cuts at: 2984 3077 2984
Size: 93 5380
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2392 2625 2792 3534 3619 4690
Size: 399 226 376 233 167 742 85 1071
Cuts at: 4690 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 376 233 226 167
85
BspEI T'CCGG_A
Cuts at: 5136 5136
Size: 5473
BspHI T'CATG_A
Cuts at: 3362 5092 3362
Size: 1730 3743
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2828 844
Size: 1984 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3360 1394
Size: 429 1537 3507
Fragments arranged by size:
3507 1537 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3968 4142 65
Size: 1183 2720 174 1396
Fragments arranged by size:
2720 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2349 2717 4117 4304 1700
Size: 110 539 368 1400 187 2869
Fragments arranged by size:
2869 1400 539 368 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5473
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5473
BssSI C'ACGA_G
Cuts at: 3533 4545 3533
Size: 1012 4461
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5473
BstBI TT'CG_AA
Cuts at: 4277 4277
Size: 5473
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5473
BstYI r'GATC_y
Cuts at: 1972 2188 2803 3170 3555 3572 5024 5110 5122
Size: 216 615 367 385 17 1452 86 12
Cuts at: 5122 5468 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 615 385 367 346 216 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2450 2450
Size: 5473
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3982
Fragments arranged by size:
3982 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4734
Fragments arranged by size:
4734 683 56
BtsI GCAGTG_nn'
Cuts at: 2989 3783 3803 2989
Size: 794 20 4659
Fragments arranged by size:
4659 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5473
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5438
DraI TTT'AAA
Cuts at: 2777 2777
Size: 5473
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4480 809
Size: 507 3164 1802
Fragments arranged by size:
3164 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2217 2715 2920
Size: 54 1425 50 275 18 387 498 205
Cuts at: 2920 3809 5438 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 498 387 275 205 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2715 2920 1537
Size: 275 903 205 4090
Fragments arranged by size:
4090 903 275 205
EarI CTCTTCn'nnn_
Cuts at: 1353 2510 3403 1353
Size: 1157 893 3423
Fragments arranged by size:
3423 1157 893
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4060 4434 4580 387
Size: 1064 138 2471 374 146 1280
Fragments arranged by size:
2471 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2623 2623
Size: 5473
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2612 2714 3536 4133 4920 5188
Size: 393 458 522 102 822 597 787 268
Cuts at: 5188 1239
Size: 1524
Fragments arranged by size:
1524 822 787 597 522 458 393 268
102
EcoRI G'AATT_C
Cuts at: 2782 3160 2782
Size: 378 5095
FspI TGC'GCA
Cuts at: 3979 3979
Size: 5473
HaeII r_GCGC'y
Cuts at: 1678 4620 1678
Size: 2942 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2325 2357 4114 4146 4188
Size: 32 135 32 753 32 1757 32 42
Cuts at: 4188 4220 1373
Size: 32 2626
Fragments arranged by size:
2626 1757 753 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2817 3063 669
Size: 988 281 879 246 3079
Fragments arranged by size:
3079 988 879 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5473
HpaI GTT'AAC
Cuts at: 3063 3063
Size: 5473
KpnI G_GTAC'C
Cuts at: 2798 2798
Size: 5473
MfeI C'AATT_G
Cuts at: 3072 3072
Size: 5473
MluI A'CGCG_T
Cuts at: 5349 5349
Size: 5473
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2356 2793 4587 4771 1305
Size: 1051 437 1794 184 2007
Fragments arranged by size:
2007 1794 1051 437 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2219 10
Size: 54 1425 343 387 3264
Fragments arranged by size:
3264 1425 387 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2468 3431 3790 3949 518
Size: 648 1302 963 359 159 2042
Fragments arranged by size:
2042 1302 963 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2065 2169 2383 2569 2590 2683 2738 2889
Size: 459 104 214 186 21 93 55 151
Cuts at: 2889 3566 4714 4959 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 459 245 214 186 151
104 93 55 21
NaeI GCC'GGC
Cuts at: 2719 2719
Size: 5473
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3982
Fragments arranged by size:
3982 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5473
NgoMIV G'CCGG_C
Cuts at: 2717 2717
Size: 5473
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5473
NotI GC'GGCC_GC
Cuts at: 2920 2920
Size: 5473
NspI r_CATG'y
Cuts at: 1132 1229 2281 2467 2831 4376 1132
Size: 97 1052 186 364 1545 2229
Fragments arranged by size:
2229 1545 1052 364 186 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5473
PciI A'CATG_T
Cuts at: 4372 4372
Size: 5473
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5473
PfoI T'CCnGG_A
Cuts at: 2637 2637
Size: 5473
PmeI GTTT'AAAC
Cuts at: 2777 2777
Size: 5473
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3542 3574 5055 5087 3542
Size: 32 1481 32 3928
Fragments arranged by size:
3928 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5473
PsiI TTA'TAA
Cuts at: 3043 3043
Size: 5473
PstI C_TGCA'G
Cuts at: 830 2247 2358 2403 2568 2825 830
Size: 1417 111 45 165 257 3478
Fragments arranged by size:
3478 1417 257 165 111 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5473
PvuII CAG'CTG
Cuts at: 2065 2383 2569 2590 2683 2738 2065
Size: 318 186 21 93 55 4800
Fragments arranged by size:
4800 318 186 93 55 21
SacI G_AGCT'C
Cuts at: 2625 2625
Size: 5473
SalI G'TCGA_C
Cuts at: 1936 2815 1936
Size: 879 4594
SbfI CC_TGCA'GG
Cuts at: 2403 2825 2403
Size: 422 5051
ScaI AGT'ACT
Cuts at: 3721 5433 3721
Size: 1712 3761
SfcI C'TryA_G
Cuts at: 826 1045 2137 2243 2354 2399 2564 2755 2821
Size: 219 1092 106 111 45 165 191 66
Cuts at: 2821 3956 4637 4828 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1092 681 219 191 191 165
111 106 66 45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5473
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5473
SmaI CCC'GGG
Cuts at: 1872 2800 1872
Size: 928 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3577 4478 4740 820
Size: 197 926 1634 901 262 1553
Fragments arranged by size:
1634 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3264 493
Size: 2771 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5473
SphI G_CATG'C
Cuts at: 1132 2831 1132
Size: 1699 3774
SspI AAT'ATT
Cuts at: 5 52 2117 3397 5
Size: 47 2065 1280 2081
Fragments arranged by size:
2081 2065 1280 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5473
StyI C'CwwG_G
Cuts at: 513 1065 2004 2912 513
Size: 552 939 908 3074
Fragments arranged by size:
3074 939 908 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3500 3517 3670 3855 4300 325
Size: 1255 1920 17 153 185 445 1498
Fragments arranged by size:
1920 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3719 5431
Size: 274 80 33 51 1443 204 1538 1712
Cuts at: 5431 96
Size: 138
Fragments arranged by size:
1712 1538 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5433 5465 5433
Size: 32 5441
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5473
XbaI T'CTAG_A
Cuts at: 2809 2809
Size: 5473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2227 2476 2227
Size: 249 5224
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5473
XmaI C'CCGG_G
Cuts at: 1870 2798 1870
Size: 928 4545
XmnI GAAnn'nnTTC
Cuts at: 3602 3602
Size: 5473
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3281 276
Size: 53 83 186 936 1747 2468
Fragments arranged by size:
2468 1747 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI