Restriction map for
pFN21AE5221
(Circular) (Six-base) MAPSORT of: pFN21AE5221.seq Check: 5223 from: 1 to: 5482
pFN21AE5221 5482 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3292 278
Size: 53 83 186 936 1756 2468
Fragments arranged by size:
2468 1756 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2825 1937
Size: 888 4594
Acc65I G'GTAC_C
Cuts at: 2721 2803 2721
Size: 82 5400
AclI AA'CG_TT
Cuts at: 1563 3609 3982 1563
Size: 2046 373 3063
Fragments arranged by size:
3063 2046 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3545 4929 5197 1632
Size: 1913 1384 268 1917
Fragments arranged by size:
1917 1913 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5285
AflIII A'CryG_T
Cuts at: 1203 4381 5358 1203
Size: 3178 977 1327
Fragments arranged by size:
3178 1327 977
AgeI A'CCGG_T
Cuts at: 4313 4313
Size: 5482
AhdI GACnn_n'nnGTC
Cuts at: 4211 4211
Size: 5482
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5482
AlwNI CAG_nnn'CTG
Cuts at: 2185 3225 4797 2185
Size: 1040 1572 2870
Fragments arranged by size:
2870 1572 1040
ApaLI G'TGCA_C
Cuts at: 3539 4695 3539
Size: 1156 4326
ApoI r'AATT_y
Cuts at: 1493 2732 2791 3017 3169 5278 1493
Size: 1239 59 226 152 2109 1697
Fragments arranged by size:
2109 1697 1239 226 152 59
AseI AT'TA_AT
Cuts at: 160 1032 4036 160
Size: 872 3004 1606
Fragments arranged by size:
3004 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
AvaI C'yCGr_G
Cuts at: 1870 1943 2807 1870
Size: 73 864 4545
Fragments arranged by size:
4545 864 73
BamHI G'GATC_C
Cuts at: 2812 3179 2812
Size: 367 5115
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2721 2803 4258 618
Size: 325 217 627 934 82 1455 1842
Fragments arranged by size:
1842 1455 934 627 325 217 82
BanII G_rGCy'C
Cuts at: 1391 2801 1391
Size: 1410 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2077 928
Size: 971 178 4333
Fragments arranged by size:
4333 971 178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3673 3707 1903
Size: 34 1736 34 3678
Fragments arranged by size:
3678 1736 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2543 2754 3374 4584 2543
Size: 211 620 1210 3441
Fragments arranged by size:
3441 1210 620 211
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5482
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4093 136
Size: 107 122 71 631 605 2421 1525
Fragments arranged by size:
2421 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5477 5477
Size: 5482
BlpI GC'TnA_GC
Cuts at: 2899 2899
Size: 5482
Bme1580I G_kGCm'C
Cuts at: 1790 3543 4699 1790
Size: 1753 1156 2573
Fragments arranged by size:
2573 1753 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5482
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2446 3213 4171
Size: 416 53 83 399 24 1029 767 958
Cuts at: 4171 442
Size: 1753
Fragments arranged by size:
1753 1029 958 767 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5482
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2206 2206
Size: 5482
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4142 1239
Size: 2903 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2428 3607 4472 4770 2428
Size: 1179 865 298 3140
Fragments arranged by size:
3140 1179 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4145 882
Size: 617 2646 2219
Fragments arranged by size:
2646 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2584 3273 493
Size: 711 1380 689 2702
Fragments arranged by size:
2702 1380 711 689
BsaBI GATnn'nnATC
Cuts at: 2498 5476 2498
Size: 2978 2504
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3289 3671 4306 275
Size: 53 83 186 936 1756 382 635 1451
Fragments arranged by size:
1756 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2727 3915 4313 4587 4734 5145 2727
Size: 1188 398 274 147 411 3064
Fragments arranged by size:
3064 1188 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5257
Fragments arranged by size:
5257 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2340 1186
Size: 859 295 4328
Fragments arranged by size:
4328 859 295
BseYI C'CCAG_C
Cuts at: 2135 4685 5181 2135
Size: 2550 496 2436
Fragments arranged by size:
2550 2436 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 2439 1163
Size: 573 462 241 4206
Fragments arranged by size:
4206 573 462 241
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2122 2932 3693 4721 1540
Size: 275 186 121 810 761 1028 2301
Fragments arranged by size:
2301 1028 810 761 275 186 121
BsiHKAI G_wGCw'C
Cuts at: 3543 3628 4699 3543
Size: 85 1071 4326
Fragments arranged by size:
4326 1071 85
BsmI GAATG_Cn'
Cuts at: 2993 3086 2993
Size: 93 5389
BsmBI CGTCTCn'nnnn_
Cuts at: 2243 2243
Size: 5482
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2801 3543 3628 4699 1391
Size: 399 1011 742 85 1071 2174
Fragments arranged by size:
2174 1071 1011 742 399 85
BspEI T'CCGG_A
Cuts at: 2727 5145 2727
Size: 2418 3064
BspHI T'CATG_A
Cuts at: 2713 3371 5101 2713
Size: 658 1730 3094
Fragments arranged by size:
3094 1730 658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2067 2837 844
Size: 1223 770 3489
Fragments arranged by size:
3489 1223 770
BsrBI CCG'CTC
Cuts at: 1394 1823 2206 3369 1394
Size: 429 383 1163 3507
Fragments arranged by size:
3507 1163 429 383
BsrDI GCAATG_nn'
Cuts at: 65 1248 3977 4151 65
Size: 1183 2729 174 1396
Fragments arranged by size:
2729 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2155 4126 4313 1700
Size: 110 345 1971 187 2869
Fragments arranged by size:
2869 1971 345 187 110
BsrGI T'GTAC_A
Cuts at: 96 2354 96
Size: 2258 3224
BssHII G'CGCG_C
Cuts at: 1925 2247 1925
Size: 322 5160
BssSI C'ACGA_G
Cuts at: 3542 4554 3542
Size: 1012 4470
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2185 2426 794
Size: 1391 241 3850
Fragments arranged by size:
3850 1391 241
BstBI TT'CG_AA
Cuts at: 4286 4286
Size: 5482
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5482
BstYI r'GATC_y
Cuts at: 1972 2446 2812 3179 3564 3581 5033 5119 5131
Size: 474 366 367 385 17 1452 86 12
Cuts at: 5131 5477 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 474 385 367 366 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2519 2519
Size: 5482
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 513
Size: 552 939 66 3925
Fragments arranged by size:
3925 939 552 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4743
Fragments arranged by size:
4743 683 56
BtsI GCAGTG_nn'
Cuts at: 2069 2427 2998 3792 3812 2069
Size: 358 571 794 20 3739
Fragments arranged by size:
3739 794 571 358 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5482
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5447
DraI TTT'AAA
Cuts at: 2786 2786
Size: 5482
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4489 809
Size: 507 3173 1802
Fragments arranged by size:
3173 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2119 2268
Size: 54 1425 50 275 18 213 76 149
Cuts at: 2268 2637 2929 3818 5447 8
Size: 369 292 889 1629 43
Fragments arranged by size:
1629 1425 889 369 292 275 213 149
76 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2119 2929 1537
Size: 275 307 810 4090
Fragments arranged by size:
4090 810 307 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2733 3412 1353
Size: 1380 679 3423
Fragments arranged by size:
3423 1380 679
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 2218 4069 4443 4589 387
Size: 1064 138 453 176 1851 374 146 1280
Fragments arranged by size:
1851 1280 1064 453 374 176 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3545 4142 4929 5197 1239
Size: 393 1913 597 787 268 1524
Fragments arranged by size:
1913 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2791 3169 2791
Size: 378 5104
FspI TGC'GCA
Cuts at: 3988 3988
Size: 5482
HaeII r_GCGC'y
Cuts at: 1678 2038 4629 1678
Size: 360 2591 2531
Fragments arranged by size:
2591 2531 360
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4123 4155 4197 4229 1373
Size: 32 135 32 2551 32 42 32 2626
Fragments arranged by size:
2626 2551 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2481 2826 3072 669
Size: 988 281 543 345 246 3079
Fragments arranged by size:
3079 988 543 345 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5482
HpaI GTT'AAC
Cuts at: 2481 3072 2481
Size: 591 4891
KpnI G_GTAC'C
Cuts at: 2725 2807 2725
Size: 82 5400
MfeI C'AATT_G
Cuts at: 3081 3081
Size: 5482
MluI A'CGCG_T
Cuts at: 5358 5358
Size: 5482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4596 4780 1305
Size: 3291 184 2007
Fragments arranged by size:
3291 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2270 2639 10
Size: 54 1425 343 438 369 2853
Fragments arranged by size:
2853 1425 438 369 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3440 3799 3958 518
Size: 648 2274 359 159 2042
Fragments arranged by size:
2274 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2643 2898 3575 4723 4968 1606
Size: 1037 255 677 1148 245 2120
Fragments arranged by size:
2120 1148 1037 677 255 245
NaeI GCC'GGC
Cuts at: 2157 2157
Size: 5482
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3991
Fragments arranged by size:
3991 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5482
NgoMIV G'CCGG_C
Cuts at: 2155 2155
Size: 5482
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5482
NotI GC'GGCC_GC
Cuts at: 2929 2929
Size: 5482
NspI r_CATG'y
Cuts at: 1132 1229 2491 2840 4385 1132
Size: 97 1262 349 1545 2229
Fragments arranged by size:
2229 1545 1262 349 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5482
PciI A'CATG_T
Cuts at: 4381 4381
Size: 5482
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5482
PmeI GTTT'AAAC
Cuts at: 2786 2786
Size: 5482
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3551 3583 5064 5096 3551
Size: 32 1481 32 3937
Fragments arranged by size:
3937 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5482
PsiI TTA'TAA
Cuts at: 2765 3052 2765
Size: 287 5195
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2099 2131 2735 2767 2099
Size: 32 604 32 4814
Fragments arranged by size:
4814 604 32 32
PstI C_TGCA'G
Cuts at: 830 2064 2593 2834 830
Size: 1234 529 241 3478
Fragments arranged by size:
3478 1234 529 241
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5482
PvuII CAG'CTG
Cuts at: 2643 2643
Size: 5482
SalI G'TCGA_C
Cuts at: 1936 2824 1936
Size: 888 4594
SbfI CC_TGCA'GG
Cuts at: 2834 2834
Size: 5482
ScaI AGT'ACT
Cuts at: 3730 5442 3730
Size: 1712 3770
SfcI C'TryA_G
Cuts at: 826 1045 2060 2589 2830 3965 4646 4837 826
Size: 219 1015 529 241 1135 681 191 1471
Fragments arranged by size:
1471 1135 1015 681 529 241 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5482
SmaI CCC'GGG
Cuts at: 1872 2809 1872
Size: 937 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2407 3586 4487 4749 820
Size: 197 926 464 1179 901 262 1553
Fragments arranged by size:
1553 1179 926 901 464 262 197
SnaBI TAC'GTA
Cuts at: 493 2584 3273 493
Size: 2091 689 2702
Fragments arranged by size:
2702 2091 689
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5482
SphI G_CATG'C
Cuts at: 1132 2840 1132
Size: 1708 3774
SspI AAT'ATT
Cuts at: 5 52 3406 5
Size: 47 3354 2081
Fragments arranged by size:
3354 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5482
StyI C'CwwG_G
Cuts at: 513 1065 2004 2561 2653 2921 513
Size: 552 939 557 92 268 3074
Fragments arranged by size:
3074 939 557 552 268 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2161 3509 3526 3679 3864 4309 325
Size: 1255 581 1348 17 153 185 445 1498
Fragments arranged by size:
1498 1348 1255 581 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2354 3728 5440
Size: 274 80 33 51 1443 377 1374 1712
Cuts at: 5440 96
Size: 138
Fragments arranged by size:
1712 1443 1374 377 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5442 5474 5442
Size: 32 5450
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5482
XbaI T'CTAG_A
Cuts at: 2818 2818
Size: 5482
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5482
XmaI C'CCGG_G
Cuts at: 1870 2807 1870
Size: 937 4545
XmnI GAAnn'nnTTC
Cuts at: 3611 3611
Size: 5482
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3290 276
Size: 53 83 186 936 1756 2468
Fragments arranged by size:
2468 1756 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI