Restriction map for
pFN21AE5220
(Circular) (Six-base) MAPSORT of: pFN21AE5220.seq Check: 9337 from: 1 to: 5383
pFN21AE5220 5383 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3193 278
Size: 53 83 186 936 1657 2468
Fragments arranged by size:
2468 1657 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2131 2247 2726 1937
Size: 194 116 479 4594
Fragments arranged by size:
4594 479 194 116
Acc65I G'GTAC_C
Cuts at: 2704 2704
Size: 5383
AclI AA'CG_TT
Cuts at: 1563 3510 3883 1563
Size: 1947 373 3063
Fragments arranged by size:
3063 1947 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3446 4830 5098 1632
Size: 1814 1384 268 1917
Fragments arranged by size:
1917 1814 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5186
AflIII A'CryG_T
Cuts at: 1203 4282 5259 1203
Size: 3079 977 1327
Fragments arranged by size:
3079 1327 977
AgeI A'CCGG_T
Cuts at: 4214 4214
Size: 5383
AhdI GACnn_n'nnGTC
Cuts at: 4112 4112
Size: 5383
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5383
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5383
AlwNI CAG_nnn'CTG
Cuts at: 3126 4698 3126
Size: 1572 3811
ApaLI G'TGCA_C
Cuts at: 3440 4596 3440
Size: 1156 4227
ApoI r'AATT_y
Cuts at: 1493 2137 2216 2380 2614 2674 2692 2918 3070
Size: 644 79 164 234 60 18 226 152
Cuts at: 3070 5179 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 644 234 226 164 152 79
60 18
AseI AT'TA_AT
Cuts at: 160 1032 3937 160
Size: 872 2905 1606
Fragments arranged by size:
2905 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5383
AvaI C'yCGr_G
Cuts at: 1870 1943 2708 1870
Size: 73 765 4545
Fragments arranged by size:
4545 765 73
BamHI G'GATC_C
Cuts at: 2713 3080 2713
Size: 367 5016
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2704 4159 618
Size: 325 217 627 917 1455 1842
Fragments arranged by size:
1842 1455 917 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2702 1391
Size: 1311 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2152 928
Size: 971 253 4159
Fragments arranged by size:
4159 971 253
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3574 3608 1903
Size: 34 50 34 1553 34 3678
Fragments arranged by size:
3678 1553 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3275 4485 3275
Size: 1210 4173
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5383
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3994 136
Size: 107 122 71 631 605 2322 1525
Fragments arranged by size:
2322 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5378 5378
Size: 5383
BlpI GC'TnA_GC
Cuts at: 2082 2800 2082
Size: 718 4665
Bme1580I G_kGCm'C
Cuts at: 1790 3444 4600 1790
Size: 1654 1156 2573
Fragments arranged by size:
2573 1654 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5383
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3114 4072 442
Size: 416 53 83 399 24 1697 958 1753
Fragments arranged by size:
1753 1697 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5383
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2039 2039
Size: 5383
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2190 4043 1239
Size: 951 1853 2579
Fragments arranged by size:
2579 1853 951
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2036 3508 4373 4671 2036
Size: 1472 865 298 2748
Fragments arranged by size:
2748 1472 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4046 882
Size: 617 2547 2219
Fragments arranged by size:
2547 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2498 3174 493
Size: 711 1294 676 2702
Fragments arranged by size:
2702 1294 711 676
BsaBI GATnn'nnATC
Cuts at: 5377 5377
Size: 5383
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3190 3572 4207 275
Size: 53 83 186 936 1657 382 635 1451
Fragments arranged by size:
1657 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3816 4214 4488 4635 5046 3816
Size: 398 274 147 411 4153
Fragments arranged by size:
4153 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5158
Fragments arranged by size:
5158 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2193 1186
Size: 1007 4376
BseYI C'CCAG_C
Cuts at: 4586 5082 4586
Size: 496 4887
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2015 1163
Size: 573 279 4531
Fragments arranged by size:
4531 573 279
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2833 3594 4622 1540
Size: 275 186 832 761 1028 2301
Fragments arranged by size:
2301 1028 832 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3444 3529 4600 3444
Size: 85 1071 4227
Fragments arranged by size:
4227 1071 85
BsmI GAATG_Cn'
Cuts at: 2894 2987 2894
Size: 93 5290
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2702 3444 3529 4600 1391
Size: 399 912 742 85 1071 2174
Fragments arranged by size:
2174 1071 912 742 399 85
BspEI T'CCGG_A
Cuts at: 5046 5046
Size: 5383
BspHI T'CATG_A
Cuts at: 3272 5002 3272
Size: 1730 3653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2738 844
Size: 1894 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3270 1394
Size: 429 1447 3507
Fragments arranged by size:
3507 1447 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2597 3878 4052 65
Size: 1183 1349 1281 174 1396
Fragments arranged by size:
1396 1349 1281 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4027 4214 1700
Size: 110 2217 187 2869
Fragments arranged by size:
2869 2217 187 110
BsrGI T'GTAC_A
Cuts at: 96 2421 96
Size: 2325 3058
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5383
BssSI C'ACGA_G
Cuts at: 3443 4455 3443
Size: 1012 4371
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5383
BstBI TT'CG_AA
Cuts at: 4187 4187
Size: 5383
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5383
BstXI CCAn_nnnn'nTGG
Cuts at: 2401 2401
Size: 5383
BstYI r'GATC_y
Cuts at: 1972 2650 2713 3080 3465 3482 4934 5020 5032
Size: 678 63 367 385 17 1452 86 12
Cuts at: 5032 5378 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 678 385 367 346 86 63
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4831
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4644
Fragments arranged by size:
4644 683 56
BtsI GCAGTG_nn'
Cuts at: 2076 2899 3693 3713 2076
Size: 823 794 20 3746
Fragments arranged by size:
3746 823 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5383
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5348
DraI TTT'AAA
Cuts at: 2679 2687 2679
Size: 8 5375
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4390 809
Size: 507 3074 1802
Fragments arranged by size:
3074 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2830 3719 5348
Size: 54 1425 50 275 18 1000 889 1629
Cuts at: 5348 8
Size: 43
Fragments arranged by size:
1629 1425 1000 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2830 1537
Size: 275 1018 4090
Fragments arranged by size:
4090 1018 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3313 1353
Size: 1960 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3970 4344 4490 387
Size: 1064 138 2381 374 146 1280
Fragments arranged by size:
2381 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2190 3446 4043 4830 5098 1239
Size: 393 558 1256 597 787 268 1524
Fragments arranged by size:
1524 1256 787 597 558 393 268
EcoRI G'AATT_C
Cuts at: 2137 2692 3070 2137
Size: 555 378 4450
Fragments arranged by size:
4450 555 378
FspI TGC'GCA
Cuts at: 3889 3889
Size: 5383
HaeII r_GCGC'y
Cuts at: 1678 4530 1678
Size: 2852 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4024 4056 4098 4130 1373
Size: 32 135 32 2452 32 42 32 2626
Fragments arranged by size:
2626 2452 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2727 2973 669
Size: 988 281 789 246 3079
Fragments arranged by size:
3079 988 789 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5383
HpaI GTT'AAC
Cuts at: 2973 2973
Size: 5383
KpnI G_GTAC'C
Cuts at: 2708 2708
Size: 5383
MfeI C'AATT_G
Cuts at: 2982 2982
Size: 5383
MluI A'CGCG_T
Cuts at: 5259 5259
Size: 5383
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2188 4497 4681 1305
Size: 883 2309 184 2007
Fragments arranged by size:
2309 2007 883 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3561
Fragments arranged by size:
3561 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2241 2500 3341 3700 3859 518
Size: 648 1075 259 841 359 159 2042
Fragments arranged by size:
2042 1075 841 648 359 259 159
MspA1I CmG'CkG
Cuts at: 1606 2799 3476 4624 4869 1606
Size: 1193 677 1148 245 2120
Fragments arranged by size:
2120 1193 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4831
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5383
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5383
NotI GC'GGCC_GC
Cuts at: 2830 2830
Size: 5383
NspI r_CATG'y
Cuts at: 1132 1229 2741 4286 1132
Size: 97 1512 1545 2229
Fragments arranged by size:
2229 1545 1512 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5383
PciI A'CATG_T
Cuts at: 4282 4282
Size: 5383
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2258 1108
Size: 1150 4233
PmeI GTTT'AAAC
Cuts at: 2687 2687
Size: 5383
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3484 4965 4997 3452
Size: 32 1481 32 3838
Fragments arranged by size:
3838 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5383
PsiI TTA'TAA
Cuts at: 2067 2222 2953 2067
Size: 155 731 4497
Fragments arranged by size:
4497 731 155
PstI C_TGCA'G
Cuts at: 830 2735 830
Size: 1905 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5383
SalI G'TCGA_C
Cuts at: 1936 2725 1936
Size: 789 4594
SbfI CC_TGCA'GG
Cuts at: 2735 2735
Size: 5383
ScaI AGT'ACT
Cuts at: 3631 5343 3631
Size: 1712 3671
SfcI C'TryA_G
Cuts at: 826 1045 2132 2339 2587 2654 2731 3866 4547
Size: 219 1087 207 248 67 77 1135 681
Cuts at: 4547 4738 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1087 681 248 219 207 191
77 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5383
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5383
SmaI CCC'GGG
Cuts at: 1872 2710 1872
Size: 838 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2051 3487 4388 4650 820
Size: 197 926 108 1436 901 262 1553
Fragments arranged by size:
1553 1436 926 901 262 197 108
SnaBI TAC'GTA
Cuts at: 493 3174 493
Size: 2681 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5383
SphI G_CATG'C
Cuts at: 1132 2741 1132
Size: 1609 3774
SspI AAT'ATT
Cuts at: 5 52 3307 5
Size: 47 3255 2081
Fragments arranged by size:
3255 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5383
StyI C'CwwG_G
Cuts at: 513 1065 2822 513
Size: 552 1757 3074
Fragments arranged by size:
3074 1757 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3410 3427 3580 3765 4210 325
Size: 1255 1830 17 153 185 445 1498
Fragments arranged by size:
1830 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2190 2421 3629
Size: 274 80 33 51 1443 213 231 1208
Cuts at: 3629 5341 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1208 274 231 213 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5343 5375 5343
Size: 32 5351
Tth111I GACn'n_nGTC
Cuts at: 1316 2551 1316
Size: 1235 4148
XbaI T'CTAG_A
Cuts at: 2719 2719
Size: 5383
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5383
XmaI C'CCGG_G
Cuts at: 1870 2708 1870
Size: 838 4545
XmnI GAAnn'nnTTC
Cuts at: 3512 3512
Size: 5383
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3191 276
Size: 53 83 186 936 1657 2468
Fragments arranged by size:
2468 1657 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI