Restriction map for
pFN21AE5215
(Circular) (Six-base) MAPSORT of: pFN21AE5215.seq Check: 4273 from: 1 to: 5428
pFN21AE5215 5428 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3238 278
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2579 2771 1937
Size: 642 192 4594
Fragments arranged by size:
4594 642 192
Acc65I G'GTAC_C
Cuts at: 2749 2749
Size: 5428
AclI AA'CG_TT
Cuts at: 1563 3555 3928 1563
Size: 1992 373 3063
Fragments arranged by size:
3063 1992 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2316 2640 3491 4875 5143 1632
Size: 684 324 851 1384 268 1917
Fragments arranged by size:
1917 1384 851 684 324 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5231
AflIII A'CryG_T
Cuts at: 1203 4327 5304 1203
Size: 3124 977 1327
Fragments arranged by size:
3124 1327 977
AgeI A'CCGG_T
Cuts at: 4259 4259
Size: 5428
AhdI GACnn_n'nnGTC
Cuts at: 4157 4157
Size: 5428
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5428
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5428
AlwNI CAG_nnn'CTG
Cuts at: 3171 4743 3171
Size: 1572 3856
ApaLI G'TGCA_C
Cuts at: 3485 4641 3485
Size: 1156 4272
ApoI r'AATT_y
Cuts at: 1493 2281 2737 2963 3115 5224 1493
Size: 788 456 226 152 2109 1697
Fragments arranged by size:
2109 1697 788 456 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3982 160
Size: 872 2950 1606
Fragments arranged by size:
2950 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
AvaI C'yCGr_G
Cuts at: 1870 1943 2753 1870
Size: 73 810 4545
Fragments arranged by size:
4545 810 73
BamHI G'GATC_C
Cuts at: 2758 3125 2758
Size: 367 5061
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2670 2749 4204 618
Size: 325 217 627 883 79 1455 1842
Fragments arranged by size:
1842 1455 883 627 325 217 79
BanII G_rGCy'C
Cuts at: 1391 2706 2747 1391
Size: 1315 41 4072
Fragments arranged by size:
4072 1315 41
BbeI G_GCGC'C
Cuts at: 2674 2674
Size: 5428
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4457
BbvCI CC'TCA_GC
Cuts at: 2009 2009
Size: 5428
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3619 3653 1903
Size: 34 50 34 1598 34 3678
Fragments arranged by size:
3678 1598 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3320 4530 3320
Size: 1210 4218
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5428
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4039 136
Size: 107 122 71 631 605 2367 1525
Fragments arranged by size:
2367 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2047 5423 2047
Size: 3376 2052
BlpI GC'TnA_GC
Cuts at: 2845 2845
Size: 5428
Bme1580I G_kGCm'C
Cuts at: 1790 3489 4645 1790
Size: 1699 1156 2573
Fragments arranged by size:
2573 1699 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5428
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2459 3159 4117
Size: 416 53 83 399 24 1042 700 958
Cuts at: 4117 442
Size: 1753
Fragments arranged by size:
1753 1042 958 700 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2436 4088 1239
Size: 1197 1652 2579
Fragments arranged by size:
2579 1652 1197
Bpu10I CC'TnA_GC
Cuts at: 2009 2009
Size: 5428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3553 4418 4716 3553
Size: 865 298 4265
Fragments arranged by size:
4265 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4091 882
Size: 617 2592 2219
Fragments arranged by size:
2592 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3219 493
Size: 711 2015 2702
Fragments arranged by size:
2702 2015 711
BsaBI GATnn'nnATC
Cuts at: 5422 5422
Size: 5428
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2671 3235 3617 4252
Size: 53 83 186 936 1138 564 382 635
Cuts at: 4252 275
Size: 1451
Fragments arranged by size:
1451 1138 936 635 564 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3861 4259 4533 4680 5091 3861
Size: 398 274 147 411 4198
Fragments arranged by size:
4198 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5203
Fragments arranged by size:
5203 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5428
BseYI C'CCAG_C
Cuts at: 2421 2585 4631 5127 2421
Size: 164 2046 496 2722
Fragments arranged by size:
2722 2046 496 164
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2396 1163
Size: 573 660 4195
Fragments arranged by size:
4195 660 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2688 2878 3639 4667 1540
Size: 275 186 687 190 761 1028 2301
Fragments arranged by size:
2301 1028 761 687 275 190 186
BsiHKAI G_wGCw'C
Cuts at: 3489 3574 4645 3489
Size: 85 1071 4272
Fragments arranged by size:
4272 1071 85
BsmI GAATG_Cn'
Cuts at: 2939 3032 2939
Size: 93 5335
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2706 2747 3489 3574 4645 1391
Size: 399 916 41 742 85 1071 2174
Fragments arranged by size:
2174 1071 916 742 399 85 41
BspEI T'CCGG_A
Cuts at: 5091 5091
Size: 5428
BspHI T'CATG_A
Cuts at: 3317 5047 3317
Size: 1730 3698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2783 844
Size: 1939 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3315 1394
Size: 429 1492 3507
Fragments arranged by size:
3507 1492 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3923 4097 65
Size: 1183 2675 174 1396
Fragments arranged by size:
2675 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4072 4259 1700
Size: 110 2262 187 2869
Fragments arranged by size:
2869 2262 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5428
BssHII G'CGCG_C
Cuts at: 1925 2721 1925
Size: 796 4632
BssSI C'ACGA_G
Cuts at: 2187 3488 4500 2187
Size: 1301 1012 3115
Fragments arranged by size:
3115 1301 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5428
BstBI TT'CG_AA
Cuts at: 4232 4232
Size: 5428
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5428
BstXI CCAn_nnnn'nTGG
Cuts at: 2472 2593 2472
Size: 121 5307
BstYI r'GATC_y
Cuts at: 1972 2047 2758 3125 3510 3527 4979 5065 5077
Size: 75 711 367 385 17 1452 86 12
Cuts at: 5077 5423 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 711 385 367 346 86 75
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4876
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2507 487
Size: 683 56 1281 3408
Fragments arranged by size:
3408 1281 683 56
BtsI GCAGTG_nn'
Cuts at: 2181 2944 3738 3758 2181
Size: 763 794 20 3851
Fragments arranged by size:
3851 794 763 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5428
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5393
DraI TTT'AAA
Cuts at: 2257 2732 2257
Size: 475 4953
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4435 809
Size: 507 3119 1802
Fragments arranged by size:
3119 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2875 3764 5393
Size: 54 1425 50 275 18 1045 889 1629
Cuts at: 5393 8
Size: 43
Fragments arranged by size:
1629 1425 1045 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2875 1537
Size: 275 1063 4090
Fragments arranged by size:
4090 1063 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2064 2073 2222 2263 2616 2625 3358 1353
Size: 711 9 149 41 353 9 733 3423
Fragments arranged by size:
3423 733 711 353 149 41 9 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4015 4389 4535 387
Size: 1064 138 2426 374 146 1280
Fragments arranged by size:
2426 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2316 2436 2640 3491 4088 4875 5143
Size: 393 684 120 204 851 597 787 268
Cuts at: 5143 1239
Size: 1524
Fragments arranged by size:
1524 851 787 684 597 393 268 204
120
EcoRI G'AATT_C
Cuts at: 2737 3115 2737
Size: 378 5050
FspI TGC'GCA
Cuts at: 3934 3934
Size: 5428
HaeII r_GCGC'y
Cuts at: 1678 2674 4575 1678
Size: 996 1901 2531
Fragments arranged by size:
2531 1901 996
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 4069 4101 4143
Size: 32 135 32 455 32 2010 32 42
Cuts at: 4143 4175 1373
Size: 32 2626
Fragments arranged by size:
2626 2010 455 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2580 2772 3018 669
Size: 988 281 642 192 246 3079
Fragments arranged by size:
3079 988 642 281 246 192
HindIII A'AGCT_T
Cuts at: 748 2260 748
Size: 1512 3916
HpaI GTT'AAC
Cuts at: 3018 3018
Size: 5428
KasI G'GCGC_C
Cuts at: 2670 2670
Size: 5428
KpnI G_GTAC'C
Cuts at: 2753 2753
Size: 5428
MfeI C'AATT_G
Cuts at: 3027 3027
Size: 5428
MluI A'CGCG_T
Cuts at: 5304 5304
Size: 5428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2708 4542 4726 1305
Size: 1403 1834 184 2007
Fragments arranged by size:
2007 1834 1403 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3606
Fragments arranged by size:
3606 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2604 3386 3745 3904 518
Size: 648 1438 782 359 159 2042
Fragments arranged by size:
2042 1438 782 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2251 2844 3521 4669 4914 1606
Size: 645 593 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 645 593 245
NarI GG'CG_CC
Cuts at: 2671 2671
Size: 5428
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4876
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5428
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5428
NotI GC'GGCC_GC
Cuts at: 2875 2875
Size: 5428
NspI r_CATG'y
Cuts at: 1132 1229 2786 4331 1132
Size: 97 1557 1545 2229
Fragments arranged by size:
2229 1557 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5428
PciI A'CATG_T
Cuts at: 4327 4327
Size: 5428
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5428
PmeI GTTT'AAAC
Cuts at: 2732 2732
Size: 5428
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3497 3529 5010 5042 3497
Size: 32 1481 32 3883
Fragments arranged by size:
3883 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5428
PsiI TTA'TAA
Cuts at: 2504 2998 2504
Size: 494 4934
PstI C_TGCA'G
Cuts at: 830 2780 830
Size: 1950 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5428
PvuII CAG'CTG
Cuts at: 2251 2251
Size: 5428
SalI G'TCGA_C
Cuts at: 1936 2578 2770 1936
Size: 642 192 4594
Fragments arranged by size:
4594 642 192
SbfI CC_TGCA'GG
Cuts at: 2780 2780
Size: 5428
ScaI AGT'ACT
Cuts at: 3676 5388 3676
Size: 1712 3716
SexAI A'CCwGG_T
Cuts at: 2235 2235
Size: 5428
SfcI C'TryA_G
Cuts at: 826 1045 2776 3911 4592 4783 826
Size: 219 1731 1135 681 191 1471
Fragments arranged by size:
1731 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2672 2672
Size: 5428
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5428
SmaI CCC'GGG
Cuts at: 1872 2755 1872
Size: 883 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3532 4433 4695 820
Size: 197 926 1589 901 262 1553
Fragments arranged by size:
1589 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3219 493
Size: 2726 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5428
SphI G_CATG'C
Cuts at: 1132 2786 1132
Size: 1654 3774
SspI AAT'ATT
Cuts at: 5 52 3352 5
Size: 47 3300 2081
Fragments arranged by size:
3300 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5428
StyI C'CwwG_G
Cuts at: 513 1065 2867 513
Size: 552 1802 3074
Fragments arranged by size:
3074 1802 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2599 3455 3472 3625 3810 4255 325
Size: 1255 1019 856 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1019 856 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3674 5386 96
Size: 274 80 33 51 1443 1697 1712 138
Fragments arranged by size:
1712 1697 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5388 5420 5388
Size: 32 5396
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5428
XbaI T'CTAG_A
Cuts at: 2764 2764
Size: 5428
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5428
XmaI C'CCGG_G
Cuts at: 1870 2753 1870
Size: 883 4545
XmnI GAAnn'nnTTC
Cuts at: 3557 3557
Size: 5428
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3236 276
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SrfI SwaI
XcmI