Restriction map for
pFN21AE5212
(Circular) (Six-base) MAPSORT of: pFN21AE5212.seq Check: 4387 from: 1 to: 5260
pFN21AE5212 5260 bp
With 182 enzymes: SgfI *
January 30, 2014 17:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2445 3070 278
Size: 53 83 186 936 909 625 2468
Fragments arranged by size:
2468 936 909 625 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2603 1937
Size: 666 4594
Acc65I G'GTAC_C
Cuts at: 2581 2581
Size: 5260
AclI AA'CG_TT
Cuts at: 1563 3387 3760 1563
Size: 1824 373 3063
Fragments arranged by size:
3063 1824 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3323 4707 4975 1632
Size: 1691 1384 268 1917
Fragments arranged by size:
1917 1691 1384 268
AfeI AGC'GCT
Cuts at: 2302 2302
Size: 5260
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5063
AflIII A'CryG_T
Cuts at: 1203 2127 4159 5136 1203
Size: 924 2032 977 1327
Fragments arranged by size:
2032 1327 977 924
AgeI A'CCGG_T
Cuts at: 4091 4091
Size: 5260
AhdI GACnn_n'nnGTC
Cuts at: 2446 3989 2446
Size: 1543 3717
AleI CACnn'nnGTG
Cuts at: 1166 2177 1166
Size: 1011 4249
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5260
AlwNI CAG_nnn'CTG
Cuts at: 2067 3003 4575 2067
Size: 936 1572 2752
Fragments arranged by size:
2752 1572 936
ApaLI G'TGCA_C
Cuts at: 3317 4473 3317
Size: 1156 4104
ApoI r'AATT_y
Cuts at: 1493 2569 2795 2947 5056 1493
Size: 1076 226 152 2109 1697
Fragments arranged by size:
2109 1697 1076 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3814 160
Size: 872 2782 1606
Fragments arranged by size:
2782 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5260
AvaI C'yCGr_G
Cuts at: 1870 1943 2080 2585 1870
Size: 73 137 505 4545
Fragments arranged by size:
4545 505 137 73
BamHI G'GATC_C
Cuts at: 2590 2957 2590
Size: 367 4893
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2581 4036 618
Size: 325 217 627 794 1455 1842
Fragments arranged by size:
1842 1455 794 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2015 2025 2579 1391
Size: 624 10 554 4072
Fragments arranged by size:
4072 624 554 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4289
BbvCI CC'TCA_GC
Cuts at: 2193 2297 2193
Size: 104 5156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3451 3485 1903
Size: 34 1514 34 3678
Fragments arranged by size:
3678 1514 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3152 4362 3152
Size: 1210 4050
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5260
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3871 136
Size: 107 122 71 631 605 2199 1525
Fragments arranged by size:
2199 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5255 5255
Size: 5260
BlpI GC'TnA_GC
Cuts at: 2677 2677
Size: 5260
Bme1580I G_kGCm'C
Cuts at: 1790 3321 4477 1790
Size: 1531 1156 2573
Fragments arranged by size:
2573 1531 1156
BmgBI CAC'GTC
Cuts at: 1318 2215 1318
Size: 897 4363
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2991 3949 442
Size: 416 53 83 399 24 1574 958 1753
Fragments arranged by size:
1753 1574 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5260
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3920 1239
Size: 2681 2579
Bpu10I CC'TnA_GC
Cuts at: 2139 2193 2297 2456 2139
Size: 54 104 159 4943
Fragments arranged by size:
4943 159 104 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3385 4250 4548 3385
Size: 865 298 4097
Fragments arranged by size:
4097 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3923 882
Size: 617 2424 2219
Fragments arranged by size:
2424 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2119 3051 493
Size: 711 915 932 2702
Fragments arranged by size:
2702 932 915 711
BsaBI GATnn'nnATC
Cuts at: 5254 5254
Size: 5260
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2442 3067 3449 4084
Size: 53 83 186 936 909 625 382 635
Cuts at: 4084 275
Size: 1451
Fragments arranged by size:
1451 936 909 635 625 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3693 4091 4365 4512 4923 3693
Size: 398 274 147 411 4030
Fragments arranged by size:
4030 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2228 2258 2534 2564 1178
Size: 30 165 30 825 30 276 30 3874
Fragments arranged by size:
3874 825 276 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5260
BseYI C'CCAG_C
Cuts at: 2508 4463 4959 2508
Size: 1955 496 2809
Fragments arranged by size:
2809 1955 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2051 1163
Size: 573 315 4372
Fragments arranged by size:
4372 573 315
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2710 3471 4499 1540
Size: 275 186 709 761 1028 2301
Fragments arranged by size:
2301 1028 761 709 275 186
BsiHKAI G_wGCw'C
Cuts at: 2025 2367 3321 3406 4477 2025
Size: 342 954 85 1071 2808
Fragments arranged by size:
2808 1071 954 342 85
BsmI GAATG_Cn'
Cuts at: 2771 2864 2771
Size: 93 5167
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2025 2367 2579 3321 3406 4477
Size: 399 225 10 342 212 742 85 1071
Cuts at: 4477 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 342 225 212 85
10
BspEI T'CCGG_A
Cuts at: 4923 4923
Size: 5260
BspHI T'CATG_A
Cuts at: 2421 3149 4879 2421
Size: 728 1730 2802
Fragments arranged by size:
2802 1730 728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2055 2190 2615 844
Size: 1211 135 425 3489
Fragments arranged by size:
3489 1211 425 135
BsrBI CCG'CTC
Cuts at: 1394 1823 2394 3147 1394
Size: 429 571 753 3507
Fragments arranged by size:
3507 753 571 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3755 3929 65
Size: 1183 2507 174 1396
Fragments arranged by size:
2507 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3904 4091 1700
Size: 110 2094 187 2869
Fragments arranged by size:
2869 2094 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5260
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5260
BssSI C'ACGA_G
Cuts at: 3320 4332 3320
Size: 1012 4248
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2067 794
Size: 1273 3987
BstBI TT'CG_AA
Cuts at: 4064 4064
Size: 5260
BstEII G'GTnAC_C
Cuts at: 1184 2101 1184
Size: 917 4343
BstYI r'GATC_y
Cuts at: 1972 2035 2112 2590 2957 3342 3359 4811 4897
Size: 63 77 478 367 385 17 1452 86
Cuts at: 4897 4909 5255 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 478 385 367 346 86 77
63 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2108 513
Size: 552 1043 3665
Fragments arranged by size:
3665 1043 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4521
Fragments arranged by size:
4521 683 56
BtsI GCAGTG_nn'
Cuts at: 2371 2776 3570 3590 2371
Size: 405 794 20 4041
Fragments arranged by size:
4041 794 405 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5260
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5225
DraI TTT'AAA
Cuts at: 2564 2564
Size: 5260
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4267 809
Size: 507 2951 1802
Fragments arranged by size:
2951 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2356 2707 3596
Size: 54 1425 50 275 18 526 351 889
Cuts at: 3596 5225 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 526 351 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2707 1537
Size: 275 895 4090
Fragments arranged by size:
4090 895 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3190 1353
Size: 1837 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3847 4221 4367 387
Size: 1064 138 2258 374 146 1280
Fragments arranged by size:
2258 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2023 2023
Size: 5260
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3323 3920 4707 4975 1239
Size: 393 1691 597 787 268 1524
Fragments arranged by size:
1691 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2202 2202
Size: 5260
EcoRI G'AATT_C
Cuts at: 2569 2947 2569
Size: 378 4882
FspI TGC'GCA
Cuts at: 3766 3766
Size: 5260
HaeII r_GCGC'y
Cuts at: 1678 2304 4407 1678
Size: 626 2103 2531
Fragments arranged by size:
2531 2103 626
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2036 2068 2186 2218 2432
Size: 32 135 32 464 32 118 32 214
Cuts at: 2432 2464 3901 3933 3975 4007 1373
Size: 32 1437 32 42 32 2626
Fragments arranged by size:
2626 1437 464 214 135 118 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2218 2604 2850 669
Size: 988 281 280 386 246 3079
Fragments arranged by size:
3079 988 386 281 280 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5260
HpaI GTT'AAC
Cuts at: 2850 2850
Size: 5260
KpnI G_GTAC'C
Cuts at: 2585 2585
Size: 5260
MfeI C'AATT_G
Cuts at: 2859 2859
Size: 5260
MluI A'CGCG_T
Cuts at: 2127 5136 2127
Size: 3009 2251
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4374 4558 1305
Size: 3069 184 2007
Fragments arranged by size:
3069 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3438
Fragments arranged by size:
3438 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 3218 3577 3736 518
Size: 648 1011 1041 359 159 2042
Fragments arranged by size:
2042 1041 1011 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2064 2110 2676 3353 4501 4746 1606
Size: 458 46 566 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 566 458 245 46
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4708
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5260
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5260
NotI GC'GGCC_GC
Cuts at: 2707 2707
Size: 5260
NspI r_CATG'y
Cuts at: 1132 1229 2618 4163 1132
Size: 97 1389 1545 2229
Fragments arranged by size:
2229 1545 1389 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5260
PciI A'CATG_T
Cuts at: 4159 4159
Size: 5260
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2341 1108
Size: 1233 4027
PmeI GTTT'AAAC
Cuts at: 2564 2564
Size: 5260
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3361 4842 4874 3329
Size: 32 1481 32 3715
Fragments arranged by size:
3715 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2202 2202
Size: 5260
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5260
PsiI TTA'TAA
Cuts at: 2830 2830
Size: 5260
PstI C_TGCA'G
Cuts at: 830 2160 2612 830
Size: 1330 452 3478
Fragments arranged by size:
3478 1330 452
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5260
SacI G_AGCT'C
Cuts at: 2025 2025
Size: 5260
SacII CC_GC'GG
Cuts at: 2111 2111
Size: 5260
SalI G'TCGA_C
Cuts at: 1936 2602 1936
Size: 666 4594
SbfI CC_TGCA'GG
Cuts at: 2612 2612
Size: 5260
ScaI AGT'ACT
Cuts at: 3508 5220 3508
Size: 1712 3548
SfcI C'TryA_G
Cuts at: 826 1045 2071 2156 2608 3743 4424 4615 826
Size: 219 1026 85 452 1135 681 191 1471
Fragments arranged by size:
1471 1135 1026 681 452 219 191 85
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5260
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5260
SmaI CCC'GGG
Cuts at: 1872 2587 1872
Size: 715 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3364 4265 4527 820
Size: 197 926 1421 901 262 1553
Fragments arranged by size:
1553 1421 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3051 493
Size: 2558 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5260
SphI G_CATG'C
Cuts at: 1132 2618 1132
Size: 1486 3774
SspI AAT'ATT
Cuts at: 5 52 3184 5
Size: 47 3132 2081
Fragments arranged by size:
3132 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5260
StyI C'CwwG_G
Cuts at: 513 1065 2538 2699 513
Size: 552 1473 161 3074
Fragments arranged by size:
3074 1473 552 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2189 3287 3304 3457 3642 4087 325
Size: 1255 609 1098 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1098 609 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3506 5218 96
Size: 274 80 33 51 1443 1529 1712 138
Fragments arranged by size:
1712 1529 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5220 5252 5220
Size: 32 5228
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5260
XbaI T'CTAG_A
Cuts at: 2596 2596
Size: 5260
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5260
XmaI C'CCGG_G
Cuts at: 1870 2585 1870
Size: 715 4545
XmnI GAAnn'nnTTC
Cuts at: 3389 3389
Size: 5260
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2443 3068 276
Size: 53 83 186 936 909 625 2468
Fragments arranged by size:
2468 936 909 625 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI