Restriction map for
pFN21AE5208
(Circular) (Six-base) MAPSORT of: pFN21AE5208.seq Check: 5470 from: 1 to: 5437
pFN21AE5208 5437 bp
With 182 enzymes: SgfI *
January 30, 2014 17:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3247 278
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2780 1937
Size: 843 4594
Acc65I G'GTAC_C
Cuts at: 2758 2758
Size: 5437
AclI AA'CG_TT
Cuts at: 1563 3564 3937 1563
Size: 2001 373 3063
Fragments arranged by size:
3063 2001 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2399 3500 4884 5152 1632
Size: 767 1101 1384 268 1917
Fragments arranged by size:
1917 1384 1101 767 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5240
AflIII A'CryG_T
Cuts at: 1203 2304 4336 5313 1203
Size: 1101 2032 977 1327
Fragments arranged by size:
2032 1327 1101 977
AgeI A'CCGG_T
Cuts at: 4268 4268
Size: 5437
AhdI GACnn_n'nnGTC
Cuts at: 4166 4166
Size: 5437
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5437
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5437
AlwNI CAG_nnn'CTG
Cuts at: 2449 2496 3180 4752 2449
Size: 47 684 1572 3134
Fragments arranged by size:
3134 1572 684 47
ApaLI G'TGCA_C
Cuts at: 3494 4650 3494
Size: 1156 4281
ApoI r'AATT_y
Cuts at: 1493 2746 2972 3124 5233 1493
Size: 1253 226 152 2109 1697
Fragments arranged by size:
2109 1697 1253 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3991 160
Size: 872 2959 1606
Fragments arranged by size:
2959 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
AvaI C'yCGr_G
Cuts at: 1870 1943 2310 2762 1870
Size: 73 367 452 4545
Fragments arranged by size:
4545 452 367 73
BamHI G'GATC_C
Cuts at: 2767 3134 2767
Size: 367 5070
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2279 2534 2666 2758
Size: 325 217 627 239 253 255 132 92
Cuts at: 2758 4213 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 325 255 253 239 217
132 92
BanII G_rGCy'C
Cuts at: 1391 2231 2422 2756 1391
Size: 840 191 334 4072
Fragments arranged by size:
4072 840 334 191
BbeI G_GCGC'C
Cuts at: 2283 2670 2283
Size: 387 5050
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2374 2408 3628 3662 1903
Size: 34 437 34 1220 34 3678
Fragments arranged by size:
3678 1220 437 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3329 4539 3329
Size: 1210 4227
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5437
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4048 136
Size: 107 122 71 631 605 2376 1525
Fragments arranged by size:
2376 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5432 5432
Size: 5437
BlpI GC'TnA_GC
Cuts at: 2421 2854 2421
Size: 433 5004
Bme1580I G_kGCm'C
Cuts at: 1790 2537 3498 4654 1790
Size: 747 961 1156 2573
Fragments arranged by size:
2573 1156 961 747
BmgBI CAC'GTC
Cuts at: 1318 2263 2305 1318
Size: 945 42 4450
Fragments arranged by size:
4450 945 42
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2615 3168 4126
Size: 416 53 83 399 24 1198 553 958
Cuts at: 4126 442
Size: 1753
Fragments arranged by size:
1753 1198 958 553 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2147 2186 4097 1239
Size: 908 39 1911 2579
Fragments arranged by size:
2579 1911 908 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3562 4427 4725 3562
Size: 865 298 4274
Fragments arranged by size:
4274 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2558 4100 882
Size: 617 1059 1542 2219
Fragments arranged by size:
2219 1542 1059 617
BsaAI yAC'GTr
Cuts at: 493 1204 3228 493
Size: 711 2024 2702
Fragments arranged by size:
2702 2024 711
BsaBI GATnn'nnATC
Cuts at: 5431 5431
Size: 5437
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2280 2667 3244 3626
Size: 53 83 186 936 747 387 577 382
Cuts at: 3626 4261 275
Size: 635 1451
Fragments arranged by size:
1451 936 747 635 577 387 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3870 4268 4542 4689 5100 3870
Size: 398 274 147 411 4207
Fragments arranged by size:
4207 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5212
Fragments arranged by size:
5212 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2423 2426 2471 2726 1186
Size: 1237 3 45 255 3897
Fragments arranged by size:
3897 1237 255 45 3
BseYI C'CCAG_C
Cuts at: 2442 4640 5136 2442
Size: 2198 496 2743
Fragments arranged by size:
2743 2198 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4864
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2398 2887 3648 4676 1540
Size: 275 186 397 489 761 1028 2301
Fragments arranged by size:
2301 1028 761 489 397 275 186
BsiHKAI G_wGCw'C
Cuts at: 3498 3583 4654 3498
Size: 85 1071 4281
Fragments arranged by size:
4281 1071 85
BsmI GAATG_Cn'
Cuts at: 2654 2948 3041 2654
Size: 294 93 5050
Fragments arranged by size:
5050 294 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2231 2422 2537 2756 3498 3583 4654
Size: 399 441 191 115 219 742 85 1071
Cuts at: 4654 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 441 399 219 191 115
85
BspEI T'CCGG_A
Cuts at: 5100 5100
Size: 5437
BspHI T'CATG_A
Cuts at: 3326 5056 3326
Size: 1730 3707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2792 844
Size: 1948 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2073 2370 2416 3324 1394
Size: 429 250 297 46 908 3507
Fragments arranged by size:
3507 908 429 297 250 46
BsrDI GCAATG_nn'
Cuts at: 65 1248 3932 4106 65
Size: 1183 2684 174 1396
Fragments arranged by size:
2684 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2397 4081 4268 1700
Size: 110 587 1684 187 2869
Fragments arranged by size:
2869 1684 587 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5437
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5437
BssSI C'ACGA_G
Cuts at: 3497 4509 3497
Size: 1012 4425
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5437
BstBI TT'CG_AA
Cuts at: 2646 4241 2646
Size: 1595 3842
BstEII G'GTnAC_C
Cuts at: 1184 2035 1184
Size: 851 4586
BstXI CCAn_nnnn'nTGG
Cuts at: 2485 2485
Size: 5437
BstYI r'GATC_y
Cuts at: 1972 2767 3134 3519 3536 4988 5074 5086 5432
Size: 795 367 385 17 1452 86 12 346
Cuts at: 5432 1972
Size: 1977
Fragments arranged by size:
1977 1452 795 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2031 2484 513
Size: 552 939 27 453 3466
Fragments arranged by size:
3466 939 552 453 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2089 487
Size: 683 56 863 3835
Fragments arranged by size:
3835 863 683 56
BtsI GCAGTG_nn'
Cuts at: 2629 2953 3747 3767 2629
Size: 324 794 20 4299
Fragments arranged by size:
4299 794 324 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5437
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5402
DraI TTT'AAA
Cuts at: 2741 2741
Size: 5437
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2020 4444 809
Size: 507 704 2424 1802
Fragments arranged by size:
2424 1802 704 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2395 2451 2884
Size: 54 1425 50 275 18 565 56 433
Cuts at: 2884 3773 5402 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 565 433 275 56 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2395 2884 1537
Size: 275 583 489 4090
Fragments arranged by size:
4090 583 489 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3367 1353
Size: 2014 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4024 4398 4544 387
Size: 1064 138 2435 374 146 1280
Fragments arranged by size:
2435 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 2186 2399 3500 4097 4884 5152
Size: 393 515 39 213 1101 597 787 268
Cuts at: 5152 1239
Size: 1524
Fragments arranged by size:
1524 1101 787 597 515 393 268 213
39
EcoNI CCTnn'n_nnAGG
Cuts at: 2453 2453
Size: 5437
EcoRI G'AATT_C
Cuts at: 2746 3124 2746
Size: 378 5059
FspI TGC'GCA
Cuts at: 3943 3943
Size: 5437
HaeII r_GCGC'y
Cuts at: 1678 2283 2449 2670 4584 1678
Size: 605 166 221 1914 2531
Fragments arranged by size:
2531 1914 605 221 166
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2291 2323 4078 4110 4152
Size: 32 135 32 719 32 1755 32 42
Cuts at: 4152 4184 1373
Size: 32 2626
Fragments arranged by size:
2626 1755 719 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2182 2781 3027 669
Size: 988 281 244 599 246 3079
Fragments arranged by size:
3079 988 599 281 246 244
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5437
HpaI GTT'AAC
Cuts at: 3027 3027
Size: 5437
KasI G'GCGC_C
Cuts at: 2279 2666 2279
Size: 387 5050
KpnI G_GTAC'C
Cuts at: 2762 2762
Size: 5437
MfeI C'AATT_G
Cuts at: 3036 3036
Size: 5437
MluI A'CGCG_T
Cuts at: 5313 5313
Size: 5437
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2706 4551 4735 1305
Size: 1401 1845 184 2007
Fragments arranged by size:
2007 1845 1401 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3615
Fragments arranged by size:
3615 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2483 2540 3395 3754 3913 518
Size: 648 843 474 57 855 359 159 2042
Fragments arranged by size:
2042 855 843 648 474 359 159 57
MspA1I CmG'CkG
Cuts at: 1606 2496 2853 3530 4678 4923 1606
Size: 890 357 677 1148 245 2120
Fragments arranged by size:
2120 1148 890 677 357 245
NarI GG'CG_CC
Cuts at: 2280 2667 2280
Size: 387 5050
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3946
Fragments arranged by size:
3946 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5437
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5437
NotI GC'GGCC_GC
Cuts at: 2884 2884
Size: 5437
NspI r_CATG'y
Cuts at: 1132 1229 2795 4340 1132
Size: 97 1566 1545 2229
Fragments arranged by size:
2229 1566 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5437
PciI A'CATG_T
Cuts at: 4336 4336
Size: 5437
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2449 1108
Size: 1341 4096
PfoI T'CCnGG_A
Cuts at: 2706 2706
Size: 5437
PmeI GTTT'AAAC
Cuts at: 2741 2741
Size: 5437
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 5019 5051 3506
Size: 32 1481 32 3892
Fragments arranged by size:
3892 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5437
PsiI TTA'TAA
Cuts at: 3007 3007
Size: 5437
PstI C_TGCA'G
Cuts at: 830 2789 830
Size: 1959 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5437
RsrII CG'GwC_CG
Cuts at: 2365 2365
Size: 5437
SalI G'TCGA_C
Cuts at: 1936 2779 1936
Size: 843 4594
SbfI CC_TGCA'GG
Cuts at: 2789 2789
Size: 5437
ScaI AGT'ACT
Cuts at: 3685 5397 3685
Size: 1712 3725
SfcI C'TryA_G
Cuts at: 826 1045 2194 2238 2785 3920 4601 4792 826
Size: 219 1149 44 547 1135 681 191 1471
Fragments arranged by size:
1471 1149 1135 681 547 219 191 44
SfoI GGC'GCC
Cuts at: 2281 2668 2281
Size: 387 5050
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5437
SmaI CCC'GGG
Cuts at: 1872 2312 2764 1872
Size: 440 452 4545
Fragments arranged by size:
4545 452 440
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3541 4442 4704 820
Size: 197 926 1598 901 262 1553
Fragments arranged by size:
1598 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3228 493
Size: 2735 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5437
SphI G_CATG'C
Cuts at: 1132 2795 1132
Size: 1663 3774
SspI AAT'ATT
Cuts at: 5 52 3361 5
Size: 47 3309 2081
Fragments arranged by size:
3309 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5437
StyI C'CwwG_G
Cuts at: 513 1065 2004 2287 2876 513
Size: 552 939 283 589 3074
Fragments arranged by size:
3074 939 589 552 283
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2044 3464 3481 3634 3819 4264 325
Size: 1255 464 1420 17 153 185 445 1498
Fragments arranged by size:
1498 1420 1255 464 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2322 3683 5395
Size: 274 80 33 51 1443 345 1361 1712
Cuts at: 5395 96
Size: 138
Fragments arranged by size:
1712 1443 1361 345 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5397 5429 5397
Size: 32 5405
Tth111I GACn'n_nGTC
Cuts at: 1316 2363 1316
Size: 1047 4390
XbaI T'CTAG_A
Cuts at: 2773 2773
Size: 5437
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2660 2660
Size: 5437
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5437
XmaI C'CCGG_G
Cuts at: 1870 2310 2762 1870
Size: 440 452 4545
Fragments arranged by size:
4545 452 440
XmnI GAAnn'nnTTC
Cuts at: 3566 3566
Size: 5437
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3245 276
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI RsrII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI