Restriction map for
pFN21AE5206
(Circular) (Six-base) MAPSORT of: pFN21AE5206.seq Check: 9519 from: 1 to: 5458
pFN21AE5206 5458 bp
With 182 enzymes: SgfI *
January 30, 2014 17:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2259 3268 278
Size: 53 83 186 936 723 1009 2468
Fragments arranged by size:
2468 1009 936 723 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2801 1937
Size: 864 4594
Acc65I G'GTAC_C
Cuts at: 2652 2779 2652
Size: 127 5331
AclI AA'CG_TT
Cuts at: 1563 3585 3958 1563
Size: 2022 373 3063
Fragments arranged by size:
3063 2022 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3521 4905 5173 1632
Size: 1889 1384 268 1917
Fragments arranged by size:
1917 1889 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5261
AflIII A'CryG_T
Cuts at: 1203 4357 5334 1203
Size: 3154 977 1327
Fragments arranged by size:
3154 1327 977
AgeI A'CCGG_T
Cuts at: 4289 4289
Size: 5458
AhdI GACnn_n'nnGTC
Cuts at: 2512 4187 2512
Size: 1675 3783
AleI CACnn'nnGTG
Cuts at: 1166 2162 1166
Size: 996 4462
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2421 2585 1184
Size: 1237 164 4057
Fragments arranged by size:
4057 1237 164
AlwNI CAG_nnn'CTG
Cuts at: 3201 4773 3201
Size: 1572 3886
ApaLI G'TGCA_C
Cuts at: 3515 4671 3515
Size: 1156 4302
ApoI r'AATT_y
Cuts at: 1493 2767 2993 3145 5254 1493
Size: 1274 226 152 2109 1697
Fragments arranged by size:
2109 1697 1274 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4012 160
Size: 872 2980 1606
Fragments arranged by size:
2980 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
AvaI C'yCGr_G
Cuts at: 1870 1943 2045 2783 1870
Size: 73 102 738 4545
Fragments arranged by size:
4545 738 102 73
BamHI G'GATC_C
Cuts at: 2788 3155 2788
Size: 367 5091
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2652 2779 4234 618
Size: 325 217 627 865 127 1455 1842
Fragments arranged by size:
1842 1455 865 627 325 217 127
BanII G_rGCy'C
Cuts at: 1391 2058 2777 1391
Size: 667 719 4072
Fragments arranged by size:
4072 719 667
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2255 2511 928
Size: 971 356 256 3875
Fragments arranged by size:
3875 971 356 256
BbvCI CC'TCA_GC
Cuts at: 2747 2747
Size: 5458
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3649 3683 1903
Size: 34 1712 34 3678
Fragments arranged by size:
3678 1712 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3350 4560 3350
Size: 1210 4248
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5458
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4069 136
Size: 107 122 71 631 605 2397 1525
Fragments arranged by size:
2397 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5453 5453
Size: 5458
BlpI GC'TnA_GC
Cuts at: 2875 2875
Size: 5458
Bme1580I G_kGCm'C
Cuts at: 1790 3519 4675 1790
Size: 1729 1156 2573
Fragments arranged by size:
2573 1729 1156
BmgBI CAC'GTC
Cuts at: 1318 2245 1318
Size: 927 4531
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3189 4147 442
Size: 416 53 83 399 24 1772 958 1753
Fragments arranged by size:
1772 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 4118 1239
Size: 1061 1818 2579
Fragments arranged by size:
2579 1818 1061
Bpu10I CC'TnA_GC
Cuts at: 2747 2747
Size: 5458
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2642 3583 4448 4746 2642
Size: 941 865 298 3354
Fragments arranged by size:
3354 941 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2279 4121 882
Size: 617 780 1842 2219
Fragments arranged by size:
2219 1842 780 617
BsaAI yAC'GTr
Cuts at: 493 1204 3249 493
Size: 711 2045 2702
Fragments arranged by size:
2702 2045 711
BsaBI GATnn'nnATC
Cuts at: 5452 5452
Size: 5458
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2256 2487 3265 3647
Size: 53 83 186 936 723 231 778 382
Cuts at: 3647 4282 275
Size: 635 1451
Fragments arranged by size:
1451 936 778 723 635 382 231 186
83 53
BsaWI w'CCGG_w
Cuts at: 3891 4289 4563 4710 5121 3891
Size: 398 274 147 411 4228
Fragments arranged by size:
4228 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5233
Fragments arranged by size:
5233 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 2297 2492 1186
Size: 1099 12 195 4152
Fragments arranged by size:
4152 1099 195 12
BseYI C'CCAG_C
Cuts at: 4661 5157 4661
Size: 496 4962
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2177 2718 1163
Size: 573 441 541 3903
Fragments arranged by size:
3903 573 541 441
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2908 3669 4697 1540
Size: 275 186 907 761 1028 2301
Fragments arranged by size:
2301 1028 907 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2058 3519 3604 4675 2058
Size: 1461 85 1071 2841
Fragments arranged by size:
2841 1461 1071 85
BsmI GAATG_Cn'
Cuts at: 2969 3062 2969
Size: 93 5365
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2777 3519 3604 4675 1391
Size: 399 268 719 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 719 399 268 85
BspEI T'CCGG_A
Cuts at: 5121 5121
Size: 5458
BspHI T'CATG_A
Cuts at: 2121 3347 5077 2121
Size: 1226 1730 2502
Fragments arranged by size:
2502 1730 1226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2199 2813 844
Size: 1355 614 3489
Fragments arranged by size:
3489 1355 614
BsrBI CCG'CTC
Cuts at: 1394 1823 3345 1394
Size: 429 1522 3507
Fragments arranged by size:
3507 1522 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3953 4127 65
Size: 1183 2705 174 1396
Fragments arranged by size:
2705 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4102 4289 1700
Size: 110 2292 187 2869
Fragments arranged by size:
2869 2292 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5458
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5458
BssSI C'ACGA_G
Cuts at: 3518 4530 3518
Size: 1012 4446
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5458
BstBI TT'CG_AA
Cuts at: 4262 4262
Size: 5458
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5458
BstYI r'GATC_y
Cuts at: 1972 2167 2788 3155 3540 3557 5009 5095 5107
Size: 195 621 367 385 17 1452 86 12
Cuts at: 5107 5453 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 621 385 367 346 195 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4906
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4719
Fragments arranged by size:
4719 683 56
BtsI GCAGTG_nn'
Cuts at: 2974 3768 3788 2974
Size: 794 20 4644
Fragments arranged by size:
4644 794 20
ClaI AT'CG_AT
Cuts at: 1554 2076 1554
Size: 522 4936
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2479 2514 529
Size: 35 1915 35 3473
Fragments arranged by size:
3473 1915 35 35
DraI TTT'AAA
Cuts at: 2762 2762
Size: 5458
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4465 809
Size: 507 3149 1802
Fragments arranged by size:
3149 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2598 2905 3794
Size: 54 1425 50 275 18 768 307 889
Cuts at: 3794 5423 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 768 307 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2905 1537
Size: 275 1093 4090
Fragments arranged by size:
4090 1093 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2123 3388 1353
Size: 770 1265 3423
Fragments arranged by size:
3423 1265 770
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4045 4419 4565 387
Size: 1064 138 2456 374 146 1280
Fragments arranged by size:
2456 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2056 2056
Size: 5458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 3521 4118 4905 5173 1239
Size: 393 668 1221 597 787 268 1524
Fragments arranged by size:
1524 1221 787 668 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2429 2429
Size: 5458
EcoO109I rG'GnC_Cy
Cuts at: 2041 2514 2041
Size: 473 4985
EcoRI G'AATT_C
Cuts at: 2767 3145 2767
Size: 378 5080
EcoRV GAT'ATC
Cuts at: 2623 2623
Size: 5458
FspI TGC'GCA
Cuts at: 3964 3964
Size: 5458
HaeII r_GCGC'y
Cuts at: 1678 2116 4605 1678
Size: 438 2489 2531
Fragments arranged by size:
2531 2489 438
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2073 2105 2498 2530 4099
Size: 32 135 32 501 32 393 32 1569
Cuts at: 4099 4131 4173 4205 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1569 501 393 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2802 3048 669
Size: 988 281 864 246 3079
Fragments arranged by size:
3079 988 864 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5458
HpaI GTT'AAC
Cuts at: 3048 3048
Size: 5458
KpnI G_GTAC'C
Cuts at: 2656 2783 2656
Size: 127 5331
MfeI C'AATT_G
Cuts at: 3057 3057
Size: 5458
MluI A'CGCG_T
Cuts at: 5334 5334
Size: 5458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2361 4572 4756 1305
Size: 1056 2211 184 2007
Fragments arranged by size:
2211 2007 1056 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2600 10
Size: 54 1425 343 768 2868
Fragments arranged by size:
2868 1425 768 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2162 3416 3775 3934 518
Size: 648 996 1254 359 159 2042
Fragments arranged by size:
2042 1254 996 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2186 2345 2702 2874 3551 4699 4944 1606
Size: 580 159 357 172 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 580 357 245 172 159
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4906
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5458
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5458
NotI GC'GGCC_GC
Cuts at: 2905 2905
Size: 5458
NspI r_CATG'y
Cuts at: 1132 1229 2816 4361 1132
Size: 97 1587 1545 2229
Fragments arranged by size:
2229 1587 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5458
PciI A'CATG_T
Cuts at: 4357 4357
Size: 5458
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5458
PfoI T'CCnGG_A
Cuts at: 2277 2529 2277
Size: 252 5206
PmeI GTTT'AAAC
Cuts at: 2762 2762
Size: 5458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3527 3559 5040 5072 3527
Size: 32 1481 32 3913
Fragments arranged by size:
3913 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2041 2514 2041
Size: 473 4985
PshAI GACnn'nnGTC
Cuts at: 1613 2255 1613
Size: 642 4816
PsiI TTA'TAA
Cuts at: 3028 3028
Size: 5458
PstI C_TGCA'G
Cuts at: 830 2810 830
Size: 1980 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5458
PvuII CAG'CTG
Cuts at: 2186 2702 2186
Size: 516 4942
SacI G_AGCT'C
Cuts at: 2058 2058
Size: 5458
SalI G'TCGA_C
Cuts at: 1936 2800 1936
Size: 864 4594
SanDI GG'GwC_CC
Cuts at: 2041 2514 2041
Size: 473 4985
SbfI CC_TGCA'GG
Cuts at: 2810 2810
Size: 5458
ScaI AGT'ACT
Cuts at: 3706 5418 3706
Size: 1712 3746
SfcI C'TryA_G
Cuts at: 826 1045 2806 3941 4622 4813 826
Size: 219 1761 1135 681 191 1471
Fragments arranged by size:
1761 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5458
SmaI CCC'GGG
Cuts at: 1872 2785 1872
Size: 913 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2657 3562 4463 4725 820
Size: 197 926 714 905 901 262 1553
Fragments arranged by size:
1553 926 905 901 714 262 197
SnaBI TAC'GTA
Cuts at: 493 3249 493
Size: 2756 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5458
SphI G_CATG'C
Cuts at: 1132 2816 1132
Size: 1684 3774
SspI AAT'ATT
Cuts at: 5 52 3382 5
Size: 47 3330 2081
Fragments arranged by size:
3330 2081 47
StuI AGG'CCT
Cuts at: 1517 2424 2643 1517
Size: 907 219 4332
Fragments arranged by size:
4332 907 219
StyI C'CwwG_G
Cuts at: 513 1065 2430 2897 513
Size: 552 1365 467 3074
Fragments arranged by size:
3074 1365 552 467
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3485 3502 3655 3840 4285 325
Size: 1255 1905 17 153 185 445 1498
Fragments arranged by size:
1905 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3704 5416 96
Size: 274 80 33 51 1443 1727 1712 138
Fragments arranged by size:
1727 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2318 2350 5418 5450 2318
Size: 32 3068 32 2326
Fragments arranged by size:
3068 2326 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5458
XbaI T'CTAG_A
Cuts at: 2794 2794
Size: 5458
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2581 2581
Size: 5458
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5458
XmaI C'CCGG_G
Cuts at: 1870 2783 1870
Size: 913 4545
XmnI GAAnn'nnTTC
Cuts at: 3587 3587
Size: 5458
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2257 3266 276
Size: 53 83 186 936 723 1009 2468
Fragments arranged by size:
2468 1009 936 723 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SapI SexAI SfiI SfoI SrfI SwaI