Restriction map for
pFN21AE5204
(Circular) (Six-base) MAPSORT of: pFN21AE5204.seq Check: 6784 from: 1 to: 5329
pFN21AE5204 5329 bp
With 182 enzymes: SgfI *
January 30, 2014 17:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3139 278
Size: 53 83 186 936 1603 2468
Fragments arranged by size:
2468 1603 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2672 1937
Size: 735 4594
Acc65I G'GTAC_C
Cuts at: 2650 2650
Size: 5329
AclI AA'CG_TT
Cuts at: 1563 3456 3829 1563
Size: 1893 373 3063
Fragments arranged by size:
3063 1893 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2379 2562 3392 4776 5044 1632
Size: 747 183 830 1384 268 1917
Fragments arranged by size:
1917 1384 830 747 268 183
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5132
AflIII A'CryG_T
Cuts at: 1203 4228 5205 1203
Size: 3025 977 1327
Fragments arranged by size:
3025 1327 977
AgeI A'CCGG_T
Cuts at: 4160 4160
Size: 5329
AhdI GACnn_n'nnGTC
Cuts at: 4058 4058
Size: 5329
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5329
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5329
AlwNI CAG_nnn'CTG
Cuts at: 2230 2402 3072 4644 2230
Size: 172 670 1572 2915
Fragments arranged by size:
2915 1572 670 172
ApaI G_GGCC'C
Cuts at: 2420 2420
Size: 5329
ApaLI G'TGCA_C
Cuts at: 3386 4542 3386
Size: 1156 4173
ApoI r'AATT_y
Cuts at: 1493 2638 2864 3016 5125 1493
Size: 1145 226 152 2109 1697
Fragments arranged by size:
2109 1697 1145 226 152
AscI GG'CGCG_CC
Cuts at: 2259 2259
Size: 5329
AseI AT'TA_AT
Cuts at: 160 1032 3883 160
Size: 872 2851 1606
Fragments arranged by size:
2851 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5329
AvaI C'yCGr_G
Cuts at: 1870 1943 2083 2362 2378 2534 2588 2654 1870
Size: 73 140 279 16 156 54 66 4545
Fragments arranged by size:
4545 279 156 140 73 66 54 16
BamHI G'GATC_C
Cuts at: 2659 3026 2659
Size: 367 4962
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2353 2650 4105 618
Size: 325 217 627 566 297 1455 1842
Fragments arranged by size:
1842 1455 627 566 325 297 217
BanII G_rGCy'C
Cuts at: 1391 2420 2429 2589 2595 2648 1391
Size: 1029 9 160 6 53 4072
Fragments arranged by size:
4072 1029 160 53 9 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4358
BbvCI CC'TCA_GC
Cuts at: 2481 2481
Size: 5329
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2343 2377 3520 3554 1903
Size: 34 406 34 1143 34 3678
Fragments arranged by size:
3678 1143 406 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3221 4431 3221
Size: 1210 4119
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5329
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2424 3940 136
Size: 107 122 71 631 605 752 1516 1525
Fragments arranged by size:
1525 1516 752 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5324 5324
Size: 5329
BlpI GC'TnA_GC
Cuts at: 2746 2746
Size: 5329
Bme1580I G_kGCm'C
Cuts at: 1790 2245 2420 3390 4546 1790
Size: 455 175 970 1156 2573
Fragments arranged by size:
2573 1156 970 455 175
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5329
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3060 4018 442
Size: 416 53 83 399 24 1643 958 1753
Fragments arranged by size:
1753 1643 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5329
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2585 3989 1239
Size: 1346 1404 2579
Fragments arranged by size:
2579 1404 1346
Bpu10I CC'TnA_GC
Cuts at: 2481 2481
Size: 5329
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3454 4319 4617 3454
Size: 865 298 4166
Fragments arranged by size:
4166 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3992 882
Size: 617 2493 2219
Fragments arranged by size:
2493 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2290 3120 493
Size: 711 1086 830 2702
Fragments arranged by size:
2702 1086 830 711
BsaBI GATnn'nnATC
Cuts at: 5323 5323
Size: 5329
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2345 3136 3518 4153
Size: 53 83 186 936 812 791 382 635
Cuts at: 4153 275
Size: 1451
Fragments arranged by size:
1451 936 812 791 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2503 2575 3762 4160 4434 4581 4992 2503
Size: 72 1187 398 274 147 411 2840
Fragments arranged by size:
2840 1187 411 398 274 147 72
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2012 2042 1178
Size: 30 165 30 609 30 4465
Fragments arranged by size:
4465 609 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2471 1186
Size: 1285 4044
BseYI C'CCAG_C
Cuts at: 2244 4532 5028 2244
Size: 2288 496 2545
Fragments arranged by size:
2545 2288 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2219 1163
Size: 573 483 4273
Fragments arranged by size:
4273 573 483
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2027 2044 2779 3540 4568 1540
Size: 275 186 26 17 735 761 1028 2301
Fragments arranged by size:
2301 1028 761 735 275 186 26 17
BsiHKAI G_wGCw'C
Cuts at: 2124 3390 3475 4546 2124
Size: 1266 85 1071 2907
Fragments arranged by size:
2907 1266 1071 85
BsmI GAATG_Cn'
Cuts at: 2840 2933 2840
Size: 93 5236
BsmBI CGTCTCn'nnnn_
Cuts at: 2060 2060
Size: 5329
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2245 2420 2429 2589 2595 2648
Size: 399 334 121 175 9 160 6 53
Cuts at: 2648 3390 3475 4546 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 334 175 160 121
85 53 9 6
BspEI T'CCGG_A
Cuts at: 4992 4992
Size: 5329
BspHI T'CATG_A
Cuts at: 3218 4948 3218
Size: 1730 3599
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2684 844
Size: 1840 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2023 2050 3216 1394
Size: 429 200 27 1166 3507
Fragments arranged by size:
3507 1166 429 200 27
BsrDI GCAATG_nn'
Cuts at: 65 1248 3824 3998 65
Size: 1183 2576 174 1396
Fragments arranged by size:
2576 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3973 4160 1700
Size: 110 2163 187 2869
Fragments arranged by size:
2869 2163 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5329
BssHII G'CGCG_C
Cuts at: 1925 2259 1925
Size: 334 4995
BssSI C'ACGA_G
Cuts at: 3389 4401 3389
Size: 1012 4317
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2150 794
Size: 1356 3973
BstBI TT'CG_AA
Cuts at: 4133 4133
Size: 5329
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5329
BstXI CCAn_nnnn'nTGG
Cuts at: 2340 2340
Size: 5329
BstYI r'GATC_y
Cuts at: 1972 2659 3026 3411 3428 4880 4966 4978 5324
Size: 687 367 385 17 1452 86 12 346
Cuts at: 5324 1972
Size: 1977
Fragments arranged by size:
1977 1452 687 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2421 2421
Size: 5329
BtgI C'CryG_G
Cuts at: 513 1065 2004 2253 513
Size: 552 939 249 3589
Fragments arranged by size:
3589 939 552 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4590
Fragments arranged by size:
4590 683 56
BtsI GCAGTG_nn'
Cuts at: 2153 2845 3639 3659 2153
Size: 692 794 20 3823
Fragments arranged by size:
3823 794 692 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5329
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5294
DraI TTT'AAA
Cuts at: 2633 2633
Size: 5329
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4336 809
Size: 507 3020 1802
Fragments arranged by size:
3020 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2024 2776 3665
Size: 54 1425 50 275 18 194 752 889
Cuts at: 3665 5294 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 752 275 194 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2024 2776 1537
Size: 275 212 752 4090
Fragments arranged by size:
4090 752 275 212
EarI CTCTTCn'nnn_
Cuts at: 1353 3259 1353
Size: 1906 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2093 3916 4290 4436 387
Size: 1064 138 504 1823 374 146 1280
Fragments arranged by size:
1823 1280 1064 504 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2379 2562 2585 3392 3989 4776 5044
Size: 393 747 183 23 807 597 787 268
Cuts at: 5044 1239
Size: 1524
Fragments arranged by size:
1524 807 787 747 597 393 268 183
23
EcoO109I rG'GnC_Cy
Cuts at: 2416 2417 2448 2416
Size: 1 31 5297
Fragments arranged by size:
5297 31 1
EcoRI G'AATT_C
Cuts at: 2638 3016 2638
Size: 378 4951
FspI TGC'GCA
Cuts at: 3835 3835
Size: 5329
HaeII r_GCGC'y
Cuts at: 1678 4476 1678
Size: 2798 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2012 2044 3970 4002 4044
Size: 32 135 32 440 32 1926 32 42
Cuts at: 4044 4076 1373
Size: 32 2626
Fragments arranged by size:
2626 1926 440 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2673 2919 669
Size: 988 281 735 246 3079
Fragments arranged by size:
3079 988 735 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5329
HpaI GTT'AAC
Cuts at: 2919 2919
Size: 5329
KpnI G_GTAC'C
Cuts at: 2654 2654
Size: 5329
MfeI C'AATT_G
Cuts at: 2928 2928
Size: 5329
MluI A'CGCG_T
Cuts at: 5205 5205
Size: 5329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2076 4443 4627 1305
Size: 771 2367 184 2007
Fragments arranged by size:
2367 2007 771 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3507
Fragments arranged by size:
3507 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3287 3646 3805 518
Size: 648 2121 359 159 2042
Fragments arranged by size:
2121 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2255 2745 3422 4570 4815 1606
Size: 649 490 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 649 490 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3838
Fragments arranged by size:
3838 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5329
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5329
NotI GC'GGCC_GC
Cuts at: 2776 2776
Size: 5329
NspI r_CATG'y
Cuts at: 1132 1229 2376 2687 4232 1132
Size: 97 1147 311 1545 2229
Fragments arranged by size:
2229 1545 1147 311 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5329
PciI A'CATG_T
Cuts at: 4228 4228
Size: 5329
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5329
PfoI T'CCnGG_A
Cuts at: 2444 2444
Size: 5329
PmeI GTTT'AAAC
Cuts at: 2633 2633
Size: 5329
PmlI CAC'GTG
Cuts at: 2290 2290
Size: 5329
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3398 3430 4911 4943 3398
Size: 32 1481 32 3784
Fragments arranged by size:
3784 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2448 2448
Size: 5329
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5329
PsiI TTA'TAA
Cuts at: 2899 2899
Size: 5329
PspOMI G'GGCC_C
Cuts at: 2416 2416
Size: 5329
PstI C_TGCA'G
Cuts at: 830 2076 2681 830
Size: 1246 605 3478
Fragments arranged by size:
3478 1246 605
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5329
SacII CC_GC'GG
Cuts at: 2256 2256
Size: 5329
SalI G'TCGA_C
Cuts at: 1936 2671 1936
Size: 735 4594
SanDI GG'GwC_CC
Cuts at: 2448 2448
Size: 5329
SbfI CC_TGCA'GG
Cuts at: 2681 2681
Size: 5329
ScaI AGT'ACT
Cuts at: 3577 5289 3577
Size: 1712 3617
SfcI C'TryA_G
Cuts at: 826 1045 2072 2677 3812 4493 4684 826
Size: 219 1027 605 1135 681 191 1471
Fragments arranged by size:
1471 1135 1027 681 605 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5329
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5329
SmaI CCC'GGG
Cuts at: 1872 2380 2656 1872
Size: 508 276 4545
Fragments arranged by size:
4545 508 276
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3433 4334 4596 820
Size: 197 926 1490 901 262 1553
Fragments arranged by size:
1553 1490 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3120 493
Size: 2627 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5329
SphI G_CATG'C
Cuts at: 1132 2687 1132
Size: 1555 3774
SspI AAT'ATT
Cuts at: 5 52 3253 5
Size: 47 3201 2081
Fragments arranged by size:
3201 2081 47
StuI AGG'CCT
Cuts at: 1517 2486 1517
Size: 969 4360
StyI C'CwwG_G
Cuts at: 513 1065 2004 2768 513
Size: 552 939 764 3074
Fragments arranged by size:
3074 939 764 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3356 3373 3526 3711 4156 325
Size: 1255 1776 17 153 185 445 1498
Fragments arranged by size:
1776 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3575 5287 96
Size: 274 80 33 51 1443 1598 1712 138
Fragments arranged by size:
1712 1598 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5289 5321 5289
Size: 32 5297
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5329
XbaI T'CTAG_A
Cuts at: 2665 2665
Size: 5329
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5329
XmaI C'CCGG_G
Cuts at: 1870 2378 2654 1870
Size: 508 276 4545
Fragments arranged by size:
4545 508 276
XmnI GAAnn'nnTTC
Cuts at: 3458 3458
Size: 5329
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3137 276
Size: 53 83 186 936 1603 2468
Fragments arranged by size:
2468 1603 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PspXI PsrI PvuII RsrII SacI SapI SexAI SfiI
SfoI SrfI SwaI XcmI