Restriction map for
pFN21AE5196
(Circular) (Six-base) MAPSORT of: pFN21AE5196.seq Check: 7375 from: 1 to: 5188
pFN21AE5196 5188 bp
With 182 enzymes: SgfI *
January 30, 2014 17:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2998 278
Size: 53 83 186 936 1462 2468
Fragments arranged by size:
2468 1462 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2531 1937
Size: 594 4594
Acc65I G'GTAC_C
Cuts at: 2509 2509
Size: 5188
AclI AA'CG_TT
Cuts at: 1563 3315 3688 1563
Size: 1752 373 3063
Fragments arranged by size:
3063 1752 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3251 4635 4903 1632
Size: 1619 1384 268 1917
Fragments arranged by size:
1917 1619 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4991
AflIII A'CryG_T
Cuts at: 1203 2397 4087 5064 1203
Size: 1194 1690 977 1327
Fragments arranged by size:
1690 1327 1194 977
AgeI A'CCGG_T
Cuts at: 4019 4019
Size: 5188
AhdI GACnn_n'nnGTC
Cuts at: 3917 3917
Size: 5188
AleI CACnn'nnGTG
Cuts at: 1166 2304 1166
Size: 1138 4050
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5188
AlwNI CAG_nnn'CTG
Cuts at: 2931 4503 2931
Size: 1572 3616
ApaLI G'TGCA_C
Cuts at: 3245 4401 3245
Size: 1156 4032
ApoI r'AATT_y
Cuts at: 1493 2031 2497 2723 2875 4984 1493
Size: 538 466 226 152 2109 1697
Fragments arranged by size:
2109 1697 538 466 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3742 160
Size: 872 2710 1606
Fragments arranged by size:
2710 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5188
AvaI C'yCGr_G
Cuts at: 1870 1943 2513 1870
Size: 73 570 4545
Fragments arranged by size:
4545 570 73
BamHI G'GATC_C
Cuts at: 2518 2885 2518
Size: 367 4821
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2317 2509 3964 618
Size: 325 217 627 530 192 1455 1842
Fragments arranged by size:
1842 1455 627 530 325 217 192
BanII G_rGCy'C
Cuts at: 1391 2075 2110 2507 1391
Size: 684 35 397 4072
Fragments arranged by size:
4072 684 397 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3379 3413 1903
Size: 34 1442 34 3678
Fragments arranged by size:
3678 1442 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2463 3080 4290 2463
Size: 617 1210 3361
Fragments arranged by size:
3361 1210 617
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5188
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3799 136
Size: 107 122 71 631 605 2127 1525
Fragments arranged by size:
2127 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5183 5183
Size: 5188
BlpI GC'TnA_GC
Cuts at: 2605 2605
Size: 5188
Bme1580I G_kGCm'C
Cuts at: 1790 3249 4405 1790
Size: 1459 1156 2573
Fragments arranged by size:
2573 1459 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5188
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2919 3877 442
Size: 416 53 83 399 24 1502 958 1753
Fragments arranged by size:
1753 1502 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3848 1239
Size: 2609 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3313 4178 4476 3313
Size: 865 298 4025
Fragments arranged by size:
4025 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2433 3851 882
Size: 617 934 1418 2219
Fragments arranged by size:
2219 1418 934 617
BsaAI yAC'GTr
Cuts at: 493 1204 2979 493
Size: 711 1775 2702
Fragments arranged by size:
2702 1775 711
BsaBI GATnn'nnATC
Cuts at: 5182 5182
Size: 5188
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2995 3377 4012 275
Size: 53 83 186 936 1462 382 635 1451
Fragments arranged by size:
1462 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2455 3621 4019 4293 4440 4851 2455
Size: 1166 398 274 147 411 2792
Fragments arranged by size:
2792 1166 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4963
Fragments arranged by size:
4963 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5188
BseYI C'CCAG_C
Cuts at: 2124 2145 4391 4887 2124
Size: 21 2246 496 2425
Fragments arranged by size:
2425 2246 496 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4615
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2638 3399 4427 1540
Size: 275 186 637 761 1028 2301
Fragments arranged by size:
2301 1028 761 637 275 186
BsiHKAI G_wGCw'C
Cuts at: 3249 3334 4405 3249
Size: 85 1071 4032
Fragments arranged by size:
4032 1071 85
BsmI GAATG_Cn'
Cuts at: 2699 2792 2699
Size: 93 5095
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2110 2507 3249 3334 4405 1391
Size: 399 285 35 397 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 397 285 85 35
BspEI T'CCGG_A
Cuts at: 4851 4851
Size: 5188
BspHI T'CATG_A
Cuts at: 3077 4807 3077
Size: 1730 3458
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2158 2364 2543 844
Size: 1314 206 179 3489
Fragments arranged by size:
3489 1314 206 179
BsrBI CCG'CTC
Cuts at: 1394 1823 3075 1394
Size: 429 1252 3507
Fragments arranged by size:
3507 1252 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2156 2169 3683 3857 65
Size: 1183 908 13 1514 174 1396
Fragments arranged by size:
1514 1396 1183 908 174 13
BsrFI r'CCGG_y
Cuts at: 1700 1810 3832 4019 1700
Size: 110 2022 187 2869
Fragments arranged by size:
2869 2022 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5188
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5188
BssSI C'ACGA_G
Cuts at: 3248 4260 3248
Size: 1012 4176
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5188
BstBI TT'CG_AA
Cuts at: 3992 3992
Size: 5188
BstEII G'GTnAC_C
Cuts at: 1184 2252 1184
Size: 1068 4120
BstYI r'GATC_y
Cuts at: 1972 2518 2885 3270 3287 4739 4825 4837 5183
Size: 546 367 385 17 1452 86 12 346
Cuts at: 5183 1972
Size: 1977
Fragments arranged by size:
1977 1452 546 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3697
Fragments arranged by size:
3697 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2206 487
Size: 683 56 980 3469
Fragments arranged by size:
3469 980 683 56
BtsI GCAGTG_nn'
Cuts at: 2704 3498 3518 2704
Size: 794 20 4374
Fragments arranged by size:
4374 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5188
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5153
DraI TTT'AAA
Cuts at: 2492 2492
Size: 5188
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4195 809
Size: 507 2879 1802
Fragments arranged by size:
2879 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2635 3524 5153
Size: 54 1425 50 275 18 805 889 1629
Cuts at: 5153 8
Size: 43
Fragments arranged by size:
1629 1425 889 805 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2635 1537
Size: 275 823 4090
Fragments arranged by size:
4090 823 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3118 1353
Size: 1765 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3775 4149 4295 387
Size: 1064 138 2186 374 146 1280
Fragments arranged by size:
2186 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3251 3848 4635 4903 1239
Size: 393 1619 597 787 268 1524
Fragments arranged by size:
1619 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2497 2875 2497
Size: 378 4810
FspI TGC'GCA
Cuts at: 2382 3694 2382
Size: 1312 3876
HaeII r_GCGC'y
Cuts at: 1678 4335 1678
Size: 2657 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3829 3861 3903 3935 1373
Size: 32 135 32 2257 32 42 32 2626
Fragments arranged by size:
2626 2257 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2532 2778 669
Size: 988 281 594 246 3079
Fragments arranged by size:
3079 988 594 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5188
HpaI GTT'AAC
Cuts at: 2778 2778
Size: 5188
KpnI G_GTAC'C
Cuts at: 2513 2513
Size: 5188
MfeI C'AATT_G
Cuts at: 2787 2787
Size: 5188
MluI A'CGCG_T
Cuts at: 5064 5064
Size: 5188
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4302 4486 1305
Size: 2997 184 2007
Fragments arranged by size:
2997 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3366
Fragments arranged by size:
3366 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2135 2304 3146 3505 3664 518
Size: 648 969 169 842 359 159 2042
Fragments arranged by size:
2042 969 842 648 359 169 159
MspA1I CmG'CkG
Cuts at: 1606 2604 3281 4429 4674 1606
Size: 998 677 1148 245 2120
Fragments arranged by size:
2120 1148 998 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3697
Fragments arranged by size:
3697 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5188
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5188
NotI GC'GGCC_GC
Cuts at: 2635 2635
Size: 5188
NspI r_CATG'y
Cuts at: 1132 1229 2401 2418 2546 4091 1132
Size: 97 1172 17 128 1545 2229
Fragments arranged by size:
2229 1545 1172 128 97 17
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5188
PciI A'CATG_T
Cuts at: 2397 4087 2397
Size: 1690 3498
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5188
PmeI GTTT'AAAC
Cuts at: 2492 2492
Size: 5188
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3257 3289 4770 4802 3257
Size: 32 1481 32 3643
Fragments arranged by size:
3643 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5188
PsiI TTA'TAA
Cuts at: 2758 2758
Size: 5188
PstI C_TGCA'G
Cuts at: 830 2375 2540 830
Size: 1545 165 3478
Fragments arranged by size:
3478 1545 165
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5188
SalI G'TCGA_C
Cuts at: 1936 2530 1936
Size: 594 4594
SbfI CC_TGCA'GG
Cuts at: 2540 2540
Size: 5188
ScaI AGT'ACT
Cuts at: 3436 5148 3436
Size: 1712 3476
SfcI C'TryA_G
Cuts at: 826 1045 2371 2536 3671 4352 4543 826
Size: 219 1326 165 1135 681 191 1471
Fragments arranged by size:
1471 1326 1135 681 219 191 165
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5188
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5188
SmaI CCC'GGG
Cuts at: 1872 2515 1872
Size: 643 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3292 4193 4455 820
Size: 197 926 1349 901 262 1553
Fragments arranged by size:
1553 1349 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2979 493
Size: 2486 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5188
SphI G_CATG'C
Cuts at: 1132 2546 1132
Size: 1414 3774
SspI AAT'ATT
Cuts at: 5 52 2362 3112 5
Size: 47 2310 750 2081
Fragments arranged by size:
2310 2081 750 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5188
StyI C'CwwG_G
Cuts at: 513 1065 2004 2627 513
Size: 552 939 623 3074
Fragments arranged by size:
3074 939 623 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3215 3232 3385 3570 4015 325
Size: 1255 1635 17 153 185 445 1498
Fragments arranged by size:
1635 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3434 5146 96
Size: 274 80 33 51 1443 1457 1712 138
Fragments arranged by size:
1712 1457 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2301 2333 5148 5180 2301
Size: 32 2815 32 2309
Fragments arranged by size:
2815 2309 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5188
XbaI T'CTAG_A
Cuts at: 2524 2524
Size: 5188
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2100 2100
Size: 5188
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5188
XmaI C'CCGG_G
Cuts at: 1870 2513 1870
Size: 643 4545
XmnI GAAnn'nnTTC
Cuts at: 3317 3317
Size: 5188
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2996 276
Size: 53 83 186 936 1462 2468
Fragments arranged by size:
2468 1462 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI