Restriction map for
pFN21AE5195
(Circular) (Six-base) MAPSORT of: pFN21AE5195.seq Check: 1232 from: 1 to: 5173
pFN21AE5195 5173 bp
With 182 enzymes: SgfI *
January 30, 2014 17:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2258 2258
Size: 5173
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2983 278
Size: 53 83 186 936 1447 2468
Fragments arranged by size:
2468 1447 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2516 1937
Size: 579 4594
Acc65I G'GTAC_C
Cuts at: 2494 2494
Size: 5173
AclI AA'CG_TT
Cuts at: 1563 3300 3673 1563
Size: 1737 373 3063
Fragments arranged by size:
3063 1737 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3236 4620 4888 1632
Size: 1604 1384 268 1917
Fragments arranged by size:
1917 1604 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4976
AflIII A'CryG_T
Cuts at: 1203 4072 5049 1203
Size: 2869 977 1327
Fragments arranged by size:
2869 1327 977
AgeI A'CCGG_T
Cuts at: 4004 4004
Size: 5173
AhdI GACnn_n'nnGTC
Cuts at: 3902 3902
Size: 5173
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5173
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2044 1184
Size: 860 4313
AlwNI CAG_nnn'CTG
Cuts at: 2916 4488 2916
Size: 1572 3601
ApaI G_GGCC'C
Cuts at: 2313 2313
Size: 5173
ApaLI G'TGCA_C
Cuts at: 3230 4386 3230
Size: 1156 4017
ApoI r'AATT_y
Cuts at: 1493 2124 2482 2708 2860 4969 1493
Size: 631 358 226 152 2109 1697
Fragments arranged by size:
2109 1697 631 358 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3727 160
Size: 872 2695 1606
Fragments arranged by size:
2695 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5173
AvaI C'yCGr_G
Cuts at: 1870 1943 2498 1870
Size: 73 555 4545
Fragments arranged by size:
4545 555 73
BamHI G'GATC_C
Cuts at: 2503 2870 2503
Size: 367 4806
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2494 3949 618
Size: 325 217 627 221 486 1455 1842
Fragments arranged by size:
1842 1455 627 486 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2313 2492 1391
Size: 922 179 4072
Fragments arranged by size:
4072 922 179
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3364 3398 1903
Size: 34 1427 34 3678
Fragments arranged by size:
3678 1427 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2361 3065 4275 2361
Size: 704 1210 3259
Fragments arranged by size:
3259 1210 704
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5173
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3784 136
Size: 107 122 71 631 605 2112 1525
Fragments arranged by size:
2112 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5168 5168
Size: 5173
BlpI GC'TnA_GC
Cuts at: 2590 2590
Size: 5173
Bme1580I G_kGCm'C
Cuts at: 1790 2313 3234 4390 1790
Size: 523 921 1156 2573
Fragments arranged by size:
2573 1156 921 523
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5173
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2072 2904 3862
Size: 416 53 83 399 24 655 832 958
Cuts at: 3862 442
Size: 1753
Fragments arranged by size:
1753 958 832 655 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5173
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2248 2248
Size: 5173
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2218 3833 1239
Size: 979 1615 2579
Fragments arranged by size:
2579 1615 979
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2133 3298 4163 4461 2133
Size: 1165 865 298 2845
Fragments arranged by size:
2845 1165 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3836 882
Size: 617 2337 2219
Fragments arranged by size:
2337 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2395 2964 493
Size: 711 1191 569 2702
Fragments arranged by size:
2702 1191 711 569
BsaBI GATnn'nnATC
Cuts at: 5167 5167
Size: 5173
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2980 3362 3997 275
Size: 53 83 186 936 1447 382 635 1451
Fragments arranged by size:
1451 1447 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3606 4004 4278 4425 4836 3606
Size: 398 274 147 411 3943
Fragments arranged by size:
3943 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4948
Fragments arranged by size:
4948 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5173
BseYI C'CCAG_C
Cuts at: 2053 2141 4376 4872 2053
Size: 88 2235 496 2354
Fragments arranged by size:
2354 2235 496 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2204 1163
Size: 573 468 4132
Fragments arranged by size:
4132 573 468
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2623 3384 4412 1540
Size: 275 186 622 761 1028 2301
Fragments arranged by size:
2301 1028 761 622 275 186
BsiHKAI G_wGCw'C
Cuts at: 3234 3319 4390 3234
Size: 85 1071 4017
Fragments arranged by size:
4017 1071 85
BsmI GAATG_Cn'
Cuts at: 2684 2777 2684
Size: 93 5080
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2313 2492 3234 3319 4390 1391
Size: 399 523 179 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 523 399 179 85
BspEI T'CCGG_A
Cuts at: 4836 4836
Size: 5173
BspHI T'CATG_A
Cuts at: 3062 4792 3062
Size: 1730 3443
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2258 2352 2528 844
Size: 1414 94 176 3489
Fragments arranged by size:
3489 1414 176 94
BsrBI CCG'CTC
Cuts at: 1394 1823 3060 1394
Size: 429 1237 3507
Fragments arranged by size:
3507 1237 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3668 3842 65
Size: 1183 2420 174 1396
Fragments arranged by size:
2420 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2011 3817 4004 1700
Size: 110 201 1806 187 2869
Fragments arranged by size:
2869 1806 201 187 110
BsrGI T'GTAC_A
Cuts at: 96 2452 96
Size: 2356 2817
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5173
BssSI C'ACGA_G
Cuts at: 2222 3233 4245 2222
Size: 1011 1012 3150
Fragments arranged by size:
3150 1012 1011
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5173
BstBI TT'CG_AA
Cuts at: 3977 3977
Size: 5173
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5173
BstYI r'GATC_y
Cuts at: 1972 2073 2503 2870 3255 3272 4724 4810 4822
Size: 101 430 367 385 17 1452 86 12
Cuts at: 4822 5168 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 430 385 367 346 101 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2341 2341
Size: 5173
BtgI C'CryG_G
Cuts at: 513 1065 2004 2305 513
Size: 552 939 301 3381
Fragments arranged by size:
3381 939 552 301
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4434
Fragments arranged by size:
4434 683 56
BtsI GCAGTG_nn'
Cuts at: 2689 3483 3503 2689
Size: 794 20 4359
Fragments arranged by size:
4359 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5173
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5138
DraI TTT'AAA
Cuts at: 2122 2477 2122
Size: 355 4818
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4180 809
Size: 507 2864 1802
Fragments arranged by size:
2864 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2241 2620
Size: 54 1425 50 275 18 183 228 379
Cuts at: 2620 3509 5138 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 379 275 228 183 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2620 1537
Size: 275 808 4090
Fragments arranged by size:
4090 808 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3103 1353
Size: 1750 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3760 4134 4280 387
Size: 1064 138 2171 374 146 1280
Fragments arranged by size:
2171 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2218 3236 3833 4620 4888 1239
Size: 393 586 1018 597 787 268 1524
Fragments arranged by size:
1524 1018 787 597 586 393 268
EcoRI G'AATT_C
Cuts at: 2482 2860 2482
Size: 378 4795
FspI TGC'GCA
Cuts at: 3679 3679
Size: 5173
HaeII r_GCGC'y
Cuts at: 1678 4320 1678
Size: 2642 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3814 3846 3888 3920 1373
Size: 32 135 32 2242 32 42 32 2626
Fragments arranged by size:
2626 2242 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2517 2763 669
Size: 988 281 579 246 3079
Fragments arranged by size:
3079 988 579 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5173
HpaI GTT'AAC
Cuts at: 2763 2763
Size: 5173
KpnI G_GTAC'C
Cuts at: 2498 2498
Size: 5173
MfeI C'AATT_G
Cuts at: 2772 2772
Size: 5173
MluI A'CGCG_T
Cuts at: 5049 5049
Size: 5173
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4287 4471 1305
Size: 2982 184 2007
Fragments arranged by size:
2982 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3351
Fragments arranged by size:
3351 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2113 3131 3490 3649 518
Size: 648 947 1018 359 159 2042
Fragments arranged by size:
2042 1018 947 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2589 3266 4414 4659 1606
Size: 983 677 1148 245 2120
Fragments arranged by size:
2120 1148 983 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3682
Fragments arranged by size:
3682 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5173
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5173
NotI GC'GGCC_GC
Cuts at: 2620 2620
Size: 5173
NspI r_CATG'y
Cuts at: 1132 1229 2137 2157 2531 4076 1132
Size: 97 908 20 374 1545 2229
Fragments arranged by size:
2229 1545 908 374 97 20
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5173
PciI A'CATG_T
Cuts at: 4072 4072
Size: 5173
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5173
PmeI GTTT'AAAC
Cuts at: 2477 2477
Size: 5173
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3242 3274 4755 4787 3242
Size: 32 1481 32 3628
Fragments arranged by size:
3628 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5173
PsiI TTA'TAA
Cuts at: 2743 2743
Size: 5173
PspOMI G'GGCC_C
Cuts at: 2309 2309
Size: 5173
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2348 2375 2380 2407 2348
Size: 27 5 27 5114
Fragments arranged by size:
5114 27 27 5
PstI C_TGCA'G
Cuts at: 830 2363 2525 830
Size: 1533 162 3478
Fragments arranged by size:
3478 1533 162
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5173
SalI G'TCGA_C
Cuts at: 1936 2515 1936
Size: 579 4594
SbfI CC_TGCA'GG
Cuts at: 2525 2525
Size: 5173
ScaI AGT'ACT
Cuts at: 3421 5133 3421
Size: 1712 3461
SexAI A'CCwGG_T
Cuts at: 2442 2442
Size: 5173
SfcI C'TryA_G
Cuts at: 826 1045 2359 2521 3656 4337 4528 826
Size: 219 1314 162 1135 681 191 1471
Fragments arranged by size:
1471 1314 1135 681 219 191 162
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5173
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5173
SmaI CCC'GGG
Cuts at: 1872 2500 1872
Size: 628 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2148 3277 4178 4440 820
Size: 197 926 205 1129 901 262 1553
Fragments arranged by size:
1553 1129 926 901 262 205 197
SnaBI TAC'GTA
Cuts at: 493 2395 2964 493
Size: 1902 569 2702
Fragments arranged by size:
2702 1902 569
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5173
SphI G_CATG'C
Cuts at: 1132 2157 2531 1132
Size: 1025 374 3774
Fragments arranged by size:
3774 1025 374
SspI AAT'ATT
Cuts at: 5 52 3097 5
Size: 47 3045 2081
Fragments arranged by size:
3045 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5173
StyI C'CwwG_G
Cuts at: 513 1065 2004 2094 2612 513
Size: 552 939 90 518 3074
Fragments arranged by size:
3074 939 552 518 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2053 3200 3217 3370 3555 4000 325
Size: 1255 473 1147 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1147 473 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2452 3419 5131
Size: 274 80 33 51 1443 475 967 1712
Cuts at: 5131 96
Size: 138
Fragments arranged by size:
1712 1443 967 475 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5133 5165 5133
Size: 32 5141
Tth111I GACn'n_nGTC
Cuts at: 1316 2465 1316
Size: 1149 4024
XbaI T'CTAG_A
Cuts at: 2509 2509
Size: 5173
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5173
XmaI C'CCGG_G
Cuts at: 1870 2498 1870
Size: 628 4545
XmnI GAAnn'nnTTC
Cuts at: 3302 3302
Size: 5173
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2981 276
Size: 53 83 186 936 1447 2468
Fragments arranged by size:
2468 1447 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI
PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PvuII RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI XcmI