Restriction map for
pFN21AE5194
(Circular) (Six-base) MAPSORT of: pFN21AE5194.seq Check: 6958 from: 1 to: 5167
pFN21AE5194 5167 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2977 278
Size: 53 83 186 936 1441 2468
Fragments arranged by size:
2468 1441 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2510 1937
Size: 573 4594
Acc65I G'GTAC_C
Cuts at: 2295 2326 2488 2295
Size: 31 162 4974
Fragments arranged by size:
4974 162 31
AclI AA'CG_TT
Cuts at: 1563 3294 3667 1563
Size: 1731 373 3063
Fragments arranged by size:
3063 1731 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3230 4614 4882 1632
Size: 1598 1384 268 1917
Fragments arranged by size:
1917 1598 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4970
AflIII A'CryG_T
Cuts at: 1203 4066 5043 1203
Size: 2863 977 1327
Fragments arranged by size:
2863 1327 977
AgeI A'CCGG_T
Cuts at: 3998 3998
Size: 5167
AhdI GACnn_n'nnGTC
Cuts at: 3896 3896
Size: 5167
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5167
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5167
AlwNI CAG_nnn'CTG
Cuts at: 2910 4482 2910
Size: 1572 3595
ApaLI G'TGCA_C
Cuts at: 3224 4380 3224
Size: 1156 4011
ApoI r'AATT_y
Cuts at: 1493 2476 2702 2854 4963 1493
Size: 983 226 152 2109 1697
Fragments arranged by size:
2109 1697 983 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3721 160
Size: 872 2689 1606
Fragments arranged by size:
2689 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5167
AvaI C'yCGr_G
Cuts at: 1870 1943 2492 1870
Size: 73 549 4545
Fragments arranged by size:
4545 549 73
BamHI G'GATC_C
Cuts at: 2497 2864 2497
Size: 367 4800
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2295 2326 2488 3943 618
Size: 325 217 627 508 31 162 1455 1842
Fragments arranged by size:
1842 1455 627 508 325 217 162 31
BanII G_rGCy'C
Cuts at: 1391 2486 1391
Size: 1095 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2150 928
Size: 971 251 3945
Fragments arranged by size:
3945 971 251
BbvCI CC'TCA_GC
Cuts at: 2047 2047
Size: 5167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3358 3392 1903
Size: 34 1421 34 3678
Fragments arranged by size:
3678 1421 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3059 4269 3059
Size: 1210 3957
BclI T'GATC_A
Cuts at: 1548 2406 1548
Size: 858 4309
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3778 136
Size: 107 122 71 631 605 2106 1525
Fragments arranged by size:
2106 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5162 5162
Size: 5167
BlpI GC'TnA_GC
Cuts at: 2071 2584 2071
Size: 513 4654
Bme1580I G_kGCm'C
Cuts at: 1790 3228 4384 1790
Size: 1438 1156 2573
Fragments arranged by size:
2573 1438 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5167
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2208 2898 3856
Size: 416 53 83 399 24 791 690 958
Cuts at: 3856 442
Size: 1753
Fragments arranged by size:
1753 958 791 690 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5167
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2294 3827 1239
Size: 1055 1533 2579
Fragments arranged by size:
2579 1533 1055
Bpu10I CC'TnA_GC
Cuts at: 2047 2047
Size: 5167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3292 4157 4455 3292
Size: 865 298 4004
Fragments arranged by size:
4004 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2349 3830 882
Size: 617 850 1481 2219
Fragments arranged by size:
2219 1481 850 617
BsaAI yAC'GTr
Cuts at: 493 1204 2081 2958 493
Size: 711 877 877 2702
Fragments arranged by size:
2702 877 877 711
BsaBI GATnn'nnATC
Cuts at: 2282 2405 5161 2282
Size: 123 2756 2288
Fragments arranged by size:
2756 2288 123
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2974 3356 3991 275
Size: 53 83 186 936 1441 382 635 1451
Fragments arranged by size:
1451 1441 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3600 3998 4272 4419 4830 3600
Size: 398 274 147 411 3937
Fragments arranged by size:
3937 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4942
Fragments arranged by size:
4942 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2447 1186
Size: 1261 3906
BseYI C'CCAG_C
Cuts at: 4370 4866 4370
Size: 496 4671
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4594
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2617 3378 4406 1540
Size: 275 186 616 761 1028 2301
Fragments arranged by size:
2301 1028 761 616 275 186
BsiHKAI G_wGCw'C
Cuts at: 2072 2103 3228 3313 4384 2072
Size: 31 1125 85 1071 2855
Fragments arranged by size:
2855 1125 1071 85 31
BsmI GAATG_Cn'
Cuts at: 2678 2771 2678
Size: 93 5074
BsmBI CGTCTCn'nnnn_
Cuts at: 2348 2348
Size: 5167
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2103 2486 3228 3313 4384 1391
Size: 399 282 31 383 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 383 282 85 31
BspEI T'CCGG_A
Cuts at: 4830 4830
Size: 5167
BspHI T'CATG_A
Cuts at: 3056 4786 3056
Size: 1730 3437
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2522 844
Size: 1678 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3054 1394
Size: 429 1231 3507
Fragments arranged by size:
3507 1231 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3662 3836 65
Size: 1183 2414 174 1396
Fragments arranged by size:
2414 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3811 3998 1700
Size: 110 2001 187 2869
Fragments arranged by size:
2869 2001 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5167
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5167
BssSI C'ACGA_G
Cuts at: 3227 4239 3227
Size: 1012 4155
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2444 794
Size: 1650 3517
BstBI TT'CG_AA
Cuts at: 2141 3971 2141
Size: 1830 3337
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5167
BstXI CCAn_nnnn'nTGG
Cuts at: 2180 2279 2180
Size: 99 5068
BstYI r'GATC_y
Cuts at: 1972 2400 2497 2864 3249 3266 4718 4804 4816
Size: 428 97 367 385 17 1452 86 12
Cuts at: 4816 5162 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 428 385 367 346 97 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2349 2349
Size: 5167
BtgI C'CryG_G
Cuts at: 513 1065 2004 2457 513
Size: 552 939 453 3223
Fragments arranged by size:
3223 939 552 453
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4428
Fragments arranged by size:
4428 683 56
BtsI GCAGTG_nn'
Cuts at: 2072 2303 2683 3477 3497 2072
Size: 231 380 794 20 3742
Fragments arranged by size:
3742 794 380 231 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5167
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5132
DraI TTT'AAA
Cuts at: 2471 2471
Size: 5167
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4174 809
Size: 507 2858 1802
Fragments arranged by size:
2858 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2111 2158 2614
Size: 54 1425 50 275 18 281 47 456
Cuts at: 2614 3503 5132 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 456 281 275 54 50
47 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2614 1537
Size: 275 802 4090
Fragments arranged by size:
4090 802 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2391 3097 1353
Size: 1038 706 3423
Fragments arranged by size:
3423 1038 706
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3754 4128 4274 387
Size: 1064 138 2165 374 146 1280
Fragments arranged by size:
2165 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 3230 3827 4614 4882 1239
Size: 393 662 936 597 787 268 1524
Fragments arranged by size:
1524 936 787 662 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2363 2363
Size: 5167
EcoO109I rG'GnC_Cy
Cuts at: 2043 2335 2043
Size: 292 4875
EcoRI G'AATT_C
Cuts at: 2476 2854 2476
Size: 378 4789
EcoRV GAT'ATC
Cuts at: 2218 2218
Size: 5167
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2107 2107
Size: 5167
FspI TGC'GCA
Cuts at: 3673 3673
Size: 5167
HaeII r_GCGC'y
Cuts at: 1678 4314 1678
Size: 2636 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2385 2417 3808 3840 3882
Size: 32 135 32 813 32 1391 32 42
Cuts at: 3882 3914 1373
Size: 32 2626
Fragments arranged by size:
2626 1391 813 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2229 2511 2757 669
Size: 988 281 291 282 246 3079
Fragments arranged by size:
3079 988 291 282 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5167
HpaI GTT'AAC
Cuts at: 2757 2757
Size: 5167
KpnI G_GTAC'C
Cuts at: 2299 2330 2492 2299
Size: 31 162 4974
Fragments arranged by size:
4974 162 31
MfeI C'AATT_G
Cuts at: 2766 2766
Size: 5167
MluI A'CGCG_T
Cuts at: 5043 5043
Size: 5167
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4281 4465 1305
Size: 2976 184 2007
Fragments arranged by size:
2976 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2113 10
Size: 54 1425 343 281 3064
Fragments arranged by size:
3064 1425 343 281 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3125 3484 3643 518
Size: 648 1959 359 159 2042
Fragments arranged by size:
2042 1959 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2275 2319 2441 2583 3260 4408 4653 1606
Size: 669 44 122 142 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 669 245 142 122 44
NcoI C'CATG_G
Cuts at: 513 1065 2004 2457 513
Size: 552 939 453 3223
Fragments arranged by size:
3223 939 552 453
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5167
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5167
NotI GC'GGCC_GC
Cuts at: 2614 2614
Size: 5167
NspI r_CATG'y
Cuts at: 1132 1229 2525 4070 1132
Size: 97 1296 1545 2229
Fragments arranged by size:
2229 1545 1296 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5167
PciI A'CATG_T
Cuts at: 4066 4066
Size: 5167
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5167
PfoI T'CCnGG_A
Cuts at: 2286 2310 2286
Size: 24 5143
PmeI GTTT'AAAC
Cuts at: 2471 2471
Size: 5167
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3236 3268 4749 4781 3236
Size: 32 1481 32 3622
Fragments arranged by size:
3622 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2335 2335
Size: 5167
PshAI GACnn'nnGTC
Cuts at: 1613 2340 1613
Size: 727 4440
PsiI TTA'TAA
Cuts at: 2737 2737
Size: 5167
PstI C_TGCA'G
Cuts at: 830 2519 830
Size: 1689 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5167
PvuII CAG'CTG
Cuts at: 2275 2441 2275
Size: 166 5001
SalI G'TCGA_C
Cuts at: 1936 2509 1936
Size: 573 4594
SbfI CC_TGCA'GG
Cuts at: 2519 2519
Size: 5167
ScaI AGT'ACT
Cuts at: 3415 5127 3415
Size: 1712 3455
SfcI C'TryA_G
Cuts at: 826 1045 2515 3650 4331 4522 826
Size: 219 1470 1135 681 191 1471
Fragments arranged by size:
1471 1470 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5167
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5167
SmaI CCC'GGG
Cuts at: 1872 2494 1872
Size: 622 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3271 4172 4434 820
Size: 197 926 1328 901 262 1553
Fragments arranged by size:
1553 1328 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2081 2958 493
Size: 1588 877 2702
Fragments arranged by size:
2702 1588 877
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5167
SphI G_CATG'C
Cuts at: 1132 2525 1132
Size: 1393 3774
SspI AAT'ATT
Cuts at: 5 52 3091 5
Size: 47 3039 2081
Fragments arranged by size:
3039 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5167
StyI C'CwwG_G
Cuts at: 513 1065 2004 2166 2359 2457 2606 513
Size: 552 939 162 193 98 149 3074
Fragments arranged by size:
3074 939 552 193 162 149 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3194 3211 3364 3549 3994 325
Size: 1255 1614 17 153 185 445 1498
Fragments arranged by size:
1614 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3413 5125 96
Size: 274 80 33 51 1443 1436 1712 138
Fragments arranged by size:
1712 1443 1436 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5127 5159 5127
Size: 32 5135
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5167
XbaI T'CTAG_A
Cuts at: 2503 2503
Size: 5167
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2173 2173
Size: 5167
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5167
XmaI C'CCGG_G
Cuts at: 1870 2492 1870
Size: 622 4545
XmnI GAAnn'nnTTC
Cuts at: 2267 3296 2267
Size: 1029 4138
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2975 276
Size: 53 83 186 936 1441 2468
Fragments arranged by size:
2468 1441 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstZ17I DraIII EcoICRI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI