Restriction map for
pFN21AE5171
(Circular) (Six-base) MAPSORT of: pFN21AE5171.seq Check: 3268 from: 1 to: 5188
pFN21AE5171 5188 bp
With 182 enzymes: SgfI *
January 30, 2014 17:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2998 278
Size: 53 83 186 936 1462 2468
Fragments arranged by size:
2468 1462 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2531 1937
Size: 594 4594
Acc65I G'GTAC_C
Cuts at: 2509 2509
Size: 5188
AclI AA'CG_TT
Cuts at: 1563 3315 3688 1563
Size: 1752 373 3063
Fragments arranged by size:
3063 1752 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3251 4635 4903 1632
Size: 1619 1384 268 1917
Fragments arranged by size:
1917 1619 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4991
AflIII A'CryG_T
Cuts at: 1203 4087 5064 1203
Size: 2884 977 1327
Fragments arranged by size:
2884 1327 977
AgeI A'CCGG_T
Cuts at: 4019 4019
Size: 5188
AhdI GACnn_n'nnGTC
Cuts at: 3917 3917
Size: 5188
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5188
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5188
AlwNI CAG_nnn'CTG
Cuts at: 2161 2931 4503 2161
Size: 770 1572 2846
Fragments arranged by size:
2846 1572 770
ApaLI G'TGCA_C
Cuts at: 2427 3245 4401 2427
Size: 818 1156 3214
Fragments arranged by size:
3214 1156 818
ApoI r'AATT_y
Cuts at: 1493 2497 2723 2875 4984 1493
Size: 1004 226 152 2109 1697
Fragments arranged by size:
2109 1697 1004 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3742 160
Size: 872 2710 1606
Fragments arranged by size:
2710 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5188
AvaI C'yCGr_G
Cuts at: 1870 1943 2513 1870
Size: 73 570 4545
Fragments arranged by size:
4545 570 73
BamHI G'GATC_C
Cuts at: 2481 2518 2885 2481
Size: 37 367 4784
Fragments arranged by size:
4784 367 37
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2509 3964 618
Size: 325 217 627 722 1455 1842
Fragments arranged by size:
1842 1455 722 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2118 2373 2507 1391
Size: 727 255 134 4072
Fragments arranged by size:
4072 727 255 134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3379 3413 1903
Size: 34 1442 34 3678
Fragments arranged by size:
3678 1442 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3080 4290 3080
Size: 1210 3978
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5188
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3799 136
Size: 107 122 71 631 605 2127 1525
Fragments arranged by size:
2127 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5183 5183
Size: 5188
BlpI GC'TnA_GC
Cuts at: 2288 2605 2288
Size: 317 4871
Bme1580I G_kGCm'C
Cuts at: 1790 2431 3249 4405 1790
Size: 641 818 1156 2573
Fragments arranged by size:
2573 1156 818 641
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5188
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2480 2919 3877
Size: 416 53 83 399 24 1063 439 958
Cuts at: 3877 442
Size: 1753
Fragments arranged by size:
1753 1063 958 439 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2386 3848 1239
Size: 1147 1462 2579
Fragments arranged by size:
2579 1462 1147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3313 4178 4476 3313
Size: 865 298 4025
Fragments arranged by size:
4025 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2404 3851 882
Size: 617 905 1447 2219
Fragments arranged by size:
2219 1447 905 617
BsaAI yAC'GTr
Cuts at: 493 1204 2979 493
Size: 711 1775 2702
Fragments arranged by size:
2702 1775 711
BsaBI GATnn'nnATC
Cuts at: 5182 5182
Size: 5188
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2995 3377 4012 275
Size: 53 83 186 936 1462 382 635 1451
Fragments arranged by size:
1462 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3621 4019 4293 4440 4851 3621
Size: 398 274 147 411 3958
Fragments arranged by size:
3958 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4963
Fragments arranged by size:
4963 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 2474 1186
Size: 883 405 3900
Fragments arranged by size:
3900 883 405
BseYI C'CCAG_C
Cuts at: 4391 4887 4391
Size: 496 4692
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4615
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2638 3399 4427 1540
Size: 275 186 637 761 1028 2301
Fragments arranged by size:
2301 1028 761 637 275 186
BsiHKAI G_wGCw'C
Cuts at: 2431 3249 3334 4405 2431
Size: 818 85 1071 3214
Fragments arranged by size:
3214 1071 818 85
BsmI GAATG_Cn'
Cuts at: 2699 2792 2699
Size: 93 5095
BsmBI CGTCTCn'nnnn_
Cuts at: 2337 2337
Size: 5188
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2373 2431 2507 3249 3334 4405
Size: 399 328 255 58 76 742 85 1071
Cuts at: 4405 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 328 255 85 76
58
BspEI T'CCGG_A
Cuts at: 4851 4851
Size: 5188
BspHI T'CATG_A
Cuts at: 3077 4807 3077
Size: 1730 3458
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2386 2543 844
Size: 1542 157 3489
Fragments arranged by size:
3489 1542 157
BsrBI CCG'CTC
Cuts at: 1394 1823 2059 2141 3075 1394
Size: 429 236 82 934 3507
Fragments arranged by size:
3507 934 429 236 82
BsrDI GCAATG_nn'
Cuts at: 65 1248 3683 3857 65
Size: 1183 2435 174 1396
Fragments arranged by size:
2435 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3832 4019 1700
Size: 110 2022 187 2869
Fragments arranged by size:
2869 2022 187 110
BsrGI T'GTAC_A
Cuts at: 96 2470 96
Size: 2374 2814
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5188
BssSI C'ACGA_G
Cuts at: 3248 4260 3248
Size: 1012 4176
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2161 794
Size: 1367 3821
BstBI TT'CG_AA
Cuts at: 3992 3992
Size: 5188
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5188
BstYI r'GATC_y
Cuts at: 1972 2439 2481 2518 2885 3270 3287 4739 4825
Size: 467 42 37 367 385 17 1452 86
Cuts at: 4825 4837 5183 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 467 385 367 346 86 42
37 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3697
Fragments arranged by size:
3697 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4449
Fragments arranged by size:
4449 683 56
BtsI GCAGTG_nn'
Cuts at: 2704 3498 3518 2704
Size: 794 20 4374
Fragments arranged by size:
4374 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5188
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5153
DraI TTT'AAA
Cuts at: 2492 2492
Size: 5188
DraIII CAC_nnn'GTG
Cuts at: 2356 2356
Size: 5188
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4195 809
Size: 507 2879 1802
Fragments arranged by size:
2879 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2361 2635 3524
Size: 54 1425 50 275 18 531 274 889
Cuts at: 3524 5153 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 531 275 274 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2635 1537
Size: 275 823 4090
Fragments arranged by size:
4090 823 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2173 3118 1353
Size: 820 945 3423
Fragments arranged by size:
3423 945 820
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3775 4149 4295 387
Size: 1064 138 2186 374 146 1280
Fragments arranged by size:
2186 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2386 3251 3848 4635 4903 1239
Size: 393 754 865 597 787 268 1524
Fragments arranged by size:
1524 865 787 754 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2158 2158
Size: 5188
EcoRI G'AATT_C
Cuts at: 2497 2875 2497
Size: 378 4810
FspI TGC'GCA
Cuts at: 3694 3694
Size: 5188
HaeII r_GCGC'y
Cuts at: 1678 4335 1678
Size: 2657 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3829 3861 3903 3935 1373
Size: 32 135 32 2257 32 42 32 2626
Fragments arranged by size:
2626 2257 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2532 2778 669
Size: 988 281 594 246 3079
Fragments arranged by size:
3079 988 594 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5188
HpaI GTT'AAC
Cuts at: 2778 2778
Size: 5188
KpnI G_GTAC'C
Cuts at: 2513 2513
Size: 5188
MfeI C'AATT_G
Cuts at: 2787 2787
Size: 5188
MluI A'CGCG_T
Cuts at: 5064 5064
Size: 5188
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4302 4486 1305
Size: 2997 184 2007
Fragments arranged by size:
2997 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2363 10
Size: 54 1425 343 531 2835
Fragments arranged by size:
2835 1425 531 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2204 3146 3505 3664 518
Size: 648 1038 942 359 159 2042
Fragments arranged by size:
2042 1038 942 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2326 2604 3281 4429 4674 1606
Size: 720 278 677 1148 245 2120
Fragments arranged by size:
2120 1148 720 677 278 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3697
Fragments arranged by size:
3697 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5188
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5188
NotI GC'GGCC_GC
Cuts at: 2635 2635
Size: 5188
NspI r_CATG'y
Cuts at: 1132 1229 2546 4091 1132
Size: 97 1317 1545 2229
Fragments arranged by size:
2229 1545 1317 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5188
PciI A'CATG_T
Cuts at: 4087 4087
Size: 5188
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2359 1108
Size: 1251 3937
PfoI T'CCnGG_A
Cuts at: 2402 2402
Size: 5188
PmeI GTTT'AAAC
Cuts at: 2492 2492
Size: 5188
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2021 2053 3257 3289 4770 4802 2021
Size: 32 1204 32 1481 32 2407
Fragments arranged by size:
2407 1481 1204 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5188
PsiI TTA'TAA
Cuts at: 2758 2758
Size: 5188
PstI C_TGCA'G
Cuts at: 830 2218 2540 830
Size: 1388 322 3478
Fragments arranged by size:
3478 1388 322
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5188
SalI G'TCGA_C
Cuts at: 1936 2530 1936
Size: 594 4594
SbfI CC_TGCA'GG
Cuts at: 2540 2540
Size: 5188
ScaI AGT'ACT
Cuts at: 3436 5148 3436
Size: 1712 3476
SfcI C'TryA_G
Cuts at: 826 1045 2017 2214 2422 2536 3671 4352 4543
Size: 219 972 197 208 114 1135 681 191
Cuts at: 4543 826
Size: 1471
Fragments arranged by size:
1471 1135 972 681 219 208 197 191
114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5188
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5188
SmaI CCC'GGG
Cuts at: 1872 2515 1872
Size: 643 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3292 4193 4455 820
Size: 197 926 1349 901 262 1553
Fragments arranged by size:
1553 1349 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2979 493
Size: 2486 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5188
SphI G_CATG'C
Cuts at: 1132 2546 1132
Size: 1414 3774
SspI AAT'ATT
Cuts at: 5 52 3112 5
Size: 47 3060 2081
Fragments arranged by size:
3060 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5188
StyI C'CwwG_G
Cuts at: 513 1065 2004 2627 513
Size: 552 939 623 3074
Fragments arranged by size:
3074 939 623 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2064 3215 3232 3385 3570 4015 325
Size: 1255 484 1151 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1151 484 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2244 2265 2470
Size: 274 80 33 51 1443 267 21 205
Cuts at: 2470 3434 5146 96
Size: 964 1712 138
Fragments arranged by size:
1712 1443 964 274 267 205 138 80
51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5148 5180 5148
Size: 32 5156
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5188
XbaI T'CTAG_A
Cuts at: 2524 2524
Size: 5188
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5188
XmaI C'CCGG_G
Cuts at: 1870 2513 1870
Size: 643 4545
XmnI GAAnn'nnTTC
Cuts at: 3317 3317
Size: 5188
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2996 276
Size: 53 83 186 936 1462 2468
Fragments arranged by size:
2468 1462 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI