Restriction map for
pFN21AE5170
(Circular) (Six-base) MAPSORT of: pFN21AE5170.seq Check: 2835 from: 1 to: 5191
pFN21AE5170 5191 bp
With 182 enzymes: SgfI *
January 30, 2014 17:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3001 278
Size: 53 83 186 936 1465 2468
Fragments arranged by size:
2468 1465 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2342 2534 1937
Size: 405 192 4594
Fragments arranged by size:
4594 405 192
Acc65I G'GTAC_C
Cuts at: 2512 2512
Size: 5191
AclI AA'CG_TT
Cuts at: 1563 3318 3691 1563
Size: 1755 373 3063
Fragments arranged by size:
3063 1755 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3254 4638 4906 1632
Size: 1622 1384 268 1917
Fragments arranged by size:
1917 1622 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2471 820
Size: 197 1454 3540
Fragments arranged by size:
3540 1454 197
AflIII A'CryG_T
Cuts at: 1203 2218 4090 5067 1203
Size: 1015 1872 977 1327
Fragments arranged by size:
1872 1327 1015 977
AgeI A'CCGG_T
Cuts at: 4022 4022
Size: 5191
AhdI GACnn_n'nnGTC
Cuts at: 3920 3920
Size: 5191
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5191
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2042 1184
Size: 858 4333
AlwNI CAG_nnn'CTG
Cuts at: 2934 4506 2934
Size: 1572 3619
ApaLI G'TGCA_C
Cuts at: 3248 4404 3248
Size: 1156 4035
ApoI r'AATT_y
Cuts at: 1493 2386 2500 2726 2878 4987 1493
Size: 893 114 226 152 2109 1697
Fragments arranged by size:
2109 1697 893 226 152 114
AseI AT'TA_AT
Cuts at: 160 1032 3745 160
Size: 872 2713 1606
Fragments arranged by size:
2713 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5191
AvaI C'yCGr_G
Cuts at: 1870 1943 2255 2516 1870
Size: 73 312 261 4545
Fragments arranged by size:
4545 312 261 73
BamHI G'GATC_C
Cuts at: 2521 2888 2521
Size: 367 4824
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2512 3967 618
Size: 325 217 627 725 1455 1842
Fragments arranged by size:
1842 1455 725 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2510 1391
Size: 1119 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2141 2175 3382 3416 1903
Size: 34 50 34 120 34 1207 34 3678
Fragments arranged by size:
3678 1207 120 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3083 4293 3083
Size: 1210 3981
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5191
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3802 136
Size: 107 122 71 631 605 2130 1525
Fragments arranged by size:
2130 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5186 5186
Size: 5191
BlpI GC'TnA_GC
Cuts at: 2608 2608
Size: 5191
Bme1580I G_kGCm'C
Cuts at: 1790 2337 3252 4408 1790
Size: 547 915 1156 2573
Fragments arranged by size:
2573 1156 915 547
BmgBI CAC'GTC
Cuts at: 1318 2203 1318
Size: 885 4306
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2922 3880 442
Size: 416 53 83 399 24 1505 958 1753
Fragments arranged by size:
1753 1505 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5191
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2330 2402 2414 3851 1239
Size: 1091 72 12 1437 2579
Fragments arranged by size:
2579 1437 1091 72 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3316 4181 4479 3316
Size: 865 298 4028
Fragments arranged by size:
4028 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3854 882
Size: 617 2355 2219
Fragments arranged by size:
2355 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2982 493
Size: 711 1778 2702
Fragments arranged by size:
2702 1778 711
BsaBI GATnn'nnATC
Cuts at: 5185 5185
Size: 5191
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2065 2998 3380 4015
Size: 53 83 186 936 532 933 382 635
Cuts at: 4015 275
Size: 1451
Fragments arranged by size:
1451 936 933 635 532 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3624 4022 4296 4443 4854 3624
Size: 398 274 147 411 3961
Fragments arranged by size:
3961 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2180 2210 1178
Size: 30 165 30 777 30 4159
Fragments arranged by size:
4159 777 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 2273 1186
Size: 970 117 4104
Fragments arranged by size:
4104 970 117
BseYI C'CCAG_C
Cuts at: 2146 2317 4394 4890 2146
Size: 171 2077 496 2447
Fragments arranged by size:
2447 2077 496 171
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4618
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2017 2641 3402 4430 1540
Size: 275 186 16 624 761 1028 2301
Fragments arranged by size:
2301 1028 761 624 275 186 16
BsiHKAI G_wGCw'C
Cuts at: 3252 3337 4408 3252
Size: 85 1071 4035
Fragments arranged by size:
4035 1071 85
BsmI GAATG_Cn'
Cuts at: 2702 2795 2702
Size: 93 5098
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2337 2510 3252 3337 4408 1391
Size: 399 547 173 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 547 399 173 85
BspEI T'CCGG_A
Cuts at: 4854 4854
Size: 5191
BspHI T'CATG_A
Cuts at: 3080 4810 3080
Size: 1730 3461
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2546 844
Size: 1702 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3078 1394
Size: 429 1255 3507
Fragments arranged by size:
3507 1255 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3686 3860 65
Size: 1183 2438 174 1396
Fragments arranged by size:
2438 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2279 3835 4022 1700
Size: 110 469 1556 187 2869
Fragments arranged by size:
2869 1556 469 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5191
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5191
BssSI C'ACGA_G
Cuts at: 3251 4263 3251
Size: 1012 4179
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5191
BstBI TT'CG_AA
Cuts at: 3995 3995
Size: 5191
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5191
BstYI r'GATC_y
Cuts at: 1972 2521 2888 3273 3290 4742 4828 4840 5186
Size: 549 367 385 17 1452 86 12 346
Cuts at: 5186 1972
Size: 1977
Fragments arranged by size:
1977 1452 549 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2343 2343
Size: 5191
BtgI C'CryG_G
Cuts at: 513 1065 2302 513
Size: 552 1237 3402
Fragments arranged by size:
3402 1237 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4452
Fragments arranged by size:
4452 683 56
BtsI GCAGTG_nn'
Cuts at: 2707 3501 3521 2707
Size: 794 20 4377
Fragments arranged by size:
4377 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5191
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5156
DraI TTT'AAA
Cuts at: 2495 2495
Size: 5191
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4198 809
Size: 507 2882 1802
Fragments arranged by size:
2882 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2014 2638 3527
Size: 54 1425 50 275 18 184 624 889
Cuts at: 3527 5156 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 624 275 184 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2014 2638 1537
Size: 275 202 624 4090
Fragments arranged by size:
4090 624 275 202
EarI CTCTTCn'nnn_
Cuts at: 1353 2015 3121 1353
Size: 662 1106 3423
Fragments arranged by size:
3423 1106 662
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2097 3778 4152 4298 387
Size: 1064 138 508 1681 374 146 1280
Fragments arranged by size:
1681 1280 1064 508 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2330 2402 2414 3254 3851 4638 4906
Size: 393 698 72 12 840 597 787 268
Cuts at: 4906 1239
Size: 1524
Fragments arranged by size:
1524 840 787 698 597 393 268 72
12
EcoRI G'AATT_C
Cuts at: 2386 2500 2878 2386
Size: 114 378 4699
Fragments arranged by size:
4699 378 114
FspI TGC'GCA
Cuts at: 3697 3697
Size: 5191
HaeII r_GCGC'y
Cuts at: 1678 4338 1678
Size: 2660 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2467 2499 3832 3864 3906
Size: 32 135 32 895 32 1333 32 42
Cuts at: 3906 3938 1373
Size: 32 2626
Fragments arranged by size:
2626 1333 895 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2535 2781 669
Size: 988 281 597 246 3079
Fragments arranged by size:
3079 988 597 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5191
HpaI GTT'AAC
Cuts at: 2781 2781
Size: 5191
KpnI G_GTAC'C
Cuts at: 2516 2516
Size: 5191
MfeI C'AATT_G
Cuts at: 2790 2790
Size: 5191
MluI A'CGCG_T
Cuts at: 2218 5067 2218
Size: 2849 2342
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4305 4489 1305
Size: 3000 184 2007
Fragments arranged by size:
3000 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3369
Fragments arranged by size:
3369 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2042 2235 3149 3508 3667 518
Size: 648 876 193 914 359 159 2042
Fragments arranged by size:
2042 914 876 648 359 193 159
MspA1I CmG'CkG
Cuts at: 1606 2026 2607 3284 4432 4677 1606
Size: 420 581 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 581 420 245
NaeI GCC'GGC
Cuts at: 2281 2281
Size: 5191
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4639
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5191
NgoMIV G'CCGG_C
Cuts at: 2279 2279
Size: 5191
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5191
NotI GC'GGCC_GC
Cuts at: 2638 2638
Size: 5191
NspI r_CATG'y
Cuts at: 1132 1229 2549 4094 1132
Size: 97 1320 1545 2229
Fragments arranged by size:
2229 1545 1320 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5191
PciI A'CATG_T
Cuts at: 4090 4090
Size: 5191
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2308 2437 1108
Size: 1200 129 3862
Fragments arranged by size:
3862 1200 129
PfoI T'CCnGG_A
Cuts at: 2379 2430 2379
Size: 51 5140
PmeI GTTT'AAAC
Cuts at: 2495 2495
Size: 5191
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3260 3292 4773 4805 3260
Size: 32 1481 32 3646
Fragments arranged by size:
3646 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5191
PsiI TTA'TAA
Cuts at: 2761 2761
Size: 5191
PspXI vC'TCGA_Gb
Cuts at: 2255 2255
Size: 5191
PstI C_TGCA'G
Cuts at: 830 2451 2543 830
Size: 1621 92 3478
Fragments arranged by size:
3478 1621 92
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5191
SalI G'TCGA_C
Cuts at: 1936 2533 1936
Size: 597 4594
SapI GCTCTTCn'nnn_
Cuts at: 2015 2015
Size: 5191
SbfI CC_TGCA'GG
Cuts at: 2543 2543
Size: 5191
ScaI AGT'ACT
Cuts at: 3439 5151 3439
Size: 1712 3479
SfcI C'TryA_G
Cuts at: 826 1045 2447 2539 3674 4355 4546 826
Size: 219 1402 92 1135 681 191 1471
Fragments arranged by size:
1471 1402 1135 681 219 191 92
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5191
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5191
SmaI CCC'GGG
Cuts at: 1872 2518 1872
Size: 646 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2255 2471 3295 4196 4458 820
Size: 197 926 312 216 824 901 262 1553
Fragments arranged by size:
1553 926 901 824 312 262 216 197
SnaBI TAC'GTA
Cuts at: 493 2982 493
Size: 2489 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5191
SphI G_CATG'C
Cuts at: 1132 2549 1132
Size: 1417 3774
SspI AAT'ATT
Cuts at: 5 52 3115 5
Size: 47 3063 2081
Fragments arranged by size:
3063 2081 47
StuI AGG'CCT
Cuts at: 1517 2367 2469 1517
Size: 850 102 4239
Fragments arranged by size:
4239 850 102
StyI C'CwwG_G
Cuts at: 513 1065 2295 2630 513
Size: 552 1230 335 3074
Fragments arranged by size:
3074 1230 552 335
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3218 3235 3388 3573 4018 325
Size: 1255 1638 17 153 185 445 1498
Fragments arranged by size:
1638 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3437 5149 96
Size: 274 80 33 51 1443 1460 1712 138
Fragments arranged by size:
1712 1460 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5151 5183 5151
Size: 32 5159
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5191
XbaI T'CTAG_A
Cuts at: 2527 2527
Size: 5191
XhoI C'TCGA_G
Cuts at: 1943 2255 1943
Size: 312 4879
XmaI C'CCGG_G
Cuts at: 1870 2516 1870
Size: 646 4545
XmnI GAAnn'nnTTC
Cuts at: 3320 3320
Size: 5191
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2999 276
Size: 53 83 186 936 1465 2468
Fragments arranged by size:
2468 1465 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI