Restriction map for
pFN21AE5168
(Circular) (Six-base) MAPSORT of: pFN21AE5168.seq Check: 8836 from: 1 to: 5164
pFN21AE5168 5164 bp
With 182 enzymes: SgfI *
January 30, 2014 17:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2974 278
Size: 53 83 186 936 1438 2468
Fragments arranged by size:
2468 1438 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2274 2507 1937
Size: 337 233 4594
Fragments arranged by size:
4594 337 233
Acc65I G'GTAC_C
Cuts at: 2485 2485
Size: 5164
AclI AA'CG_TT
Cuts at: 1563 3291 3664 1563
Size: 1728 373 3063
Fragments arranged by size:
3063 1728 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3227 4611 4879 1632
Size: 1595 1384 268 1917
Fragments arranged by size:
1917 1595 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4967
AflIII A'CryG_T
Cuts at: 1203 4063 5040 1203
Size: 2860 977 1327
Fragments arranged by size:
2860 1327 977
AgeI A'CCGG_T
Cuts at: 3995 3995
Size: 5164
AhdI GACnn_n'nnGTC
Cuts at: 3893 3893
Size: 5164
AleI CACnn'nnGTG
Cuts at: 1166 2378 1166
Size: 1212 3952
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5164
AlwNI CAG_nnn'CTG
Cuts at: 2083 2116 2449 2907 4479 2083
Size: 33 333 458 1572 2768
Fragments arranged by size:
2768 1572 458 333 33
ApaI G_GGCC'C
Cuts at: 2037 2037
Size: 5164
ApaLI G'TGCA_C
Cuts at: 3221 4377 3221
Size: 1156 4008
ApoI r'AATT_y
Cuts at: 1493 2210 2308 2473 2699 2851 4960 1493
Size: 717 98 165 226 152 2109 1697
Fragments arranged by size:
2109 1697 717 226 165 152 98
AseI AT'TA_AT
Cuts at: 160 1032 3718 160
Size: 872 2686 1606
Fragments arranged by size:
2686 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5164
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2489 1870
Size: 73 93 453 4545
Fragments arranged by size:
4545 453 93 73
BamHI G'GATC_C
Cuts at: 2494 2861 2494
Size: 367 4797
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2485 3940 618
Size: 325 217 627 698 1455 1842
Fragments arranged by size:
1842 1455 698 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2037 2483 1391
Size: 646 446 4072
Fragments arranged by size:
4072 646 446
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3355 3389 1903
Size: 34 1418 34 3678
Fragments arranged by size:
3678 1418 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3056 4266 3056
Size: 1210 3954
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5164
BfrBI ATG'CAT
Cuts at: 2154 2154
Size: 5164
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3775 136
Size: 107 122 71 631 605 2103 1525
Fragments arranged by size:
2103 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5159 5159
Size: 5164
BlpI GC'TnA_GC
Cuts at: 2581 2581
Size: 5164
Bme1580I G_kGCm'C
Cuts at: 1790 2037 3225 4381 1790
Size: 247 1188 1156 2573
Fragments arranged by size:
2573 1188 1156 247
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5164
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2282 2895 3853
Size: 416 53 83 399 24 865 613 958
Cuts at: 3853 442
Size: 1753
Fragments arranged by size:
1753 958 865 613 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2453 3824 1239
Size: 1214 1371 2579
Fragments arranged by size:
2579 1371 1214
Bpu10I CC'TnA_GC
Cuts at: 2043 2043
Size: 5164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3289 4154 4452 3289
Size: 865 298 4001
Fragments arranged by size:
4001 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3827 882
Size: 617 2328 2219
Fragments arranged by size:
2328 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2955 493
Size: 711 1751 2702
Fragments arranged by size:
2702 1751 711
BsaBI GATnn'nnATC
Cuts at: 5158 5158
Size: 5164
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2971 3353 3988 275
Size: 53 83 186 936 1438 382 635 1451
Fragments arranged by size:
1451 1438 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3597 3995 4269 4416 4827 3597
Size: 398 274 147 411 3934
Fragments arranged by size:
3934 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2429 2459 1178
Size: 30 165 30 1026 30 3883
Fragments arranged by size:
3883 1026 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2417 2450 1186
Size: 1231 33 3900
Fragments arranged by size:
3900 1231 33
BseYI C'CCAG_C
Cuts at: 4367 4863 4367
Size: 496 4668
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2370 2462 1163
Size: 573 634 92 3865
Fragments arranged by size:
3865 634 573 92
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2614 3375 4403 1540
Size: 275 186 613 761 1028 2301
Fragments arranged by size:
2301 1028 761 613 275 186
BsiHKAI G_wGCw'C
Cuts at: 3225 3310 4381 3225
Size: 85 1071 4008
Fragments arranged by size:
4008 1071 85
BsmI GAATG_Cn'
Cuts at: 2675 2768 2675
Size: 93 5071
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2483 3225 3310 4381 1391
Size: 399 247 446 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 446 399 247 85
BspEI T'CCGG_A
Cuts at: 4827 4827
Size: 5164
BspHI T'CATG_A
Cuts at: 3053 4783 3053
Size: 1730 3434
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2519 844
Size: 1675 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3051 1394
Size: 429 1228 3507
Fragments arranged by size:
3507 1228 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3659 3833 65
Size: 1183 2411 174 1396
Fragments arranged by size:
2411 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3808 3995 1700
Size: 110 1998 187 2869
Fragments arranged by size:
2869 1998 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5164
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5164
BssSI C'ACGA_G
Cuts at: 3224 4236 3224
Size: 1012 4152
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2449 794
Size: 1655 3509
BstBI TT'CG_AA
Cuts at: 3968 3968
Size: 5164
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5164
BstYI r'GATC_y
Cuts at: 1972 2023 2494 2861 3246 3263 4715 4801 4813
Size: 51 471 367 385 17 1452 86 12
Cuts at: 4813 5159 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 471 385 367 346 86 51
17 12
BstZ17I GTA'TAC
Cuts at: 2275 2275
Size: 5164
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4612
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2089 487
Size: 683 56 863 3562
Fragments arranged by size:
3562 863 683 56
BtsI GCAGTG_nn'
Cuts at: 2258 2680 3474 3494 2258
Size: 422 794 20 3928
Fragments arranged by size:
3928 794 422 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5164
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5129
DraI TTT'AAA
Cuts at: 2468 2468
Size: 5164
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4171 809
Size: 507 2855 1802
Fragments arranged by size:
2855 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2611 3500 5129
Size: 54 1425 50 275 18 781 889 1629
Cuts at: 5129 8
Size: 43
Fragments arranged by size:
1629 1425 889 781 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2611 1537
Size: 275 799 4090
Fragments arranged by size:
4090 799 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3094 1353
Size: 1741 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2063 3751 4125 4271 387
Size: 1064 138 474 1688 374 146 1280
Fragments arranged by size:
1688 1280 1064 474 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2453 3227 3824 4611 4879 1239
Size: 393 821 774 597 787 268 1524
Fragments arranged by size:
1524 821 787 774 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2435 2435
Size: 5164
EcoRI G'AATT_C
Cuts at: 2473 2851 2473
Size: 378 4786
FspI TGC'GCA
Cuts at: 3670 3670
Size: 5164
HaeII r_GCGC'y
Cuts at: 1678 2371 4311 1678
Size: 693 1940 2531
Fragments arranged by size:
2531 1940 693
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2429 2461 3805 3837 3879
Size: 32 135 32 857 32 1344 32 42
Cuts at: 3879 3911 1373
Size: 32 2626
Fragments arranged by size:
2626 1344 857 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2092 2218 2508 2754 669
Size: 988 281 154 126 290 246 3079
Fragments arranged by size:
3079 988 290 281 246 154 126
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5164
HpaI GTT'AAC
Cuts at: 2754 2754
Size: 5164
KpnI G_GTAC'C
Cuts at: 2489 2489
Size: 5164
MfeI C'AATT_G
Cuts at: 2763 2763
Size: 5164
MluI A'CGCG_T
Cuts at: 5040 5040
Size: 5164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4278 4462 1305
Size: 2973 184 2007
Fragments arranged by size:
2973 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3342
Fragments arranged by size:
3342 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2378 3122 3481 3640 518
Size: 648 1212 744 359 159 2042
Fragments arranged by size:
2042 1212 744 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2580 3257 4405 4650 1606
Size: 974 677 1148 245 2120
Fragments arranged by size:
2120 1148 974 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4612
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5164
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5164
NotI GC'GGCC_GC
Cuts at: 2611 2611
Size: 5164
NsiI A_TGCA'T
Cuts at: 2156 2156
Size: 5164
NspI r_CATG'y
Cuts at: 1132 1229 2522 4067 1132
Size: 97 1293 1545 2229
Fragments arranged by size:
2229 1545 1293 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5164
PciI A'CATG_T
Cuts at: 4063 4063
Size: 5164
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5164
PmeI GTTT'AAAC
Cuts at: 2468 2468
Size: 5164
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3233 3265 4746 4778 3233
Size: 32 1481 32 3619
Fragments arranged by size:
3619 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5164
PsiI TTA'TAA
Cuts at: 2734 2734
Size: 5164
PspOMI G'GGCC_C
Cuts at: 2033 2033
Size: 5164
PstI C_TGCA'G
Cuts at: 830 2516 830
Size: 1686 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5164
SalI G'TCGA_C
Cuts at: 1936 2506 1936
Size: 570 4594
SbfI CC_TGCA'GG
Cuts at: 2516 2516
Size: 5164
ScaI AGT'ACT
Cuts at: 3412 5124 3412
Size: 1712 3452
SfcI C'TryA_G
Cuts at: 826 1045 2512 3647 4328 4519 826
Size: 219 1467 1135 681 191 1471
Fragments arranged by size:
1471 1467 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5164
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5164
SmaI CCC'GGG
Cuts at: 1872 2491 1872
Size: 619 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3268 4169 4431 820
Size: 197 926 1325 901 262 1553
Fragments arranged by size:
1553 1325 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2955 493
Size: 2462 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5164
SphI G_CATG'C
Cuts at: 1132 2522 1132
Size: 1390 3774
SspI AAT'ATT
Cuts at: 5 52 3088 5
Size: 47 3036 2081
Fragments arranged by size:
3036 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5164
StyI C'CwwG_G
Cuts at: 513 1065 2603 513
Size: 552 1538 3074
Fragments arranged by size:
3074 1538 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2319 2336 3191 3208 3361 3546 3991
Size: 1255 739 17 855 17 153 185 445
Cuts at: 3991 325
Size: 1498
Fragments arranged by size:
1498 1255 855 739 445 185 153 17
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3410 5122 96
Size: 274 80 33 51 1443 1433 1712 138
Fragments arranged by size:
1712 1443 1433 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5124 5156 5124
Size: 32 5132
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5164
XbaI T'CTAG_A
Cuts at: 2500 2500
Size: 5164
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5164
XmaI C'CCGG_G
Cuts at: 1870 2489 1870
Size: 619 4545
XmnI GAAnn'nnTTC
Cuts at: 3293 3293
Size: 5164
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2972 276
Size: 53 83 186 936 1438 2468
Fragments arranged by size:
2468 1438 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI