Restriction map for
pFN21AE5167
(Circular) (Six-base) MAPSORT of: pFN21AE5167.seq Check: 4338 from: 1 to: 5173
pFN21AE5167 5173 bp
With 182 enzymes: SgfI *
January 30, 2014 17:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2288 2288
Size: 5173
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2983 278
Size: 53 83 186 936 1447 2468
Fragments arranged by size:
2468 1447 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2516 1937
Size: 579 4594
Acc65I G'GTAC_C
Cuts at: 2361 2494 2361
Size: 133 5040
AclI AA'CG_TT
Cuts at: 1563 3300 3673 1563
Size: 1737 373 3063
Fragments arranged by size:
3063 1737 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3236 4620 4888 1632
Size: 1604 1384 268 1917
Fragments arranged by size:
1917 1604 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4976
AflIII A'CryG_T
Cuts at: 1203 4072 5049 1203
Size: 2869 977 1327
Fragments arranged by size:
2869 1327 977
AgeI A'CCGG_T
Cuts at: 4004 4004
Size: 5173
AhdI GACnn_n'nnGTC
Cuts at: 3902 3902
Size: 5173
AleI CACnn'nnGTG
Cuts at: 1166 2225 1166
Size: 1059 4114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5173
AlwNI CAG_nnn'CTG
Cuts at: 2916 4488 2916
Size: 1572 3601
ApaLI G'TGCA_C
Cuts at: 2228 3230 4386 2228
Size: 1002 1156 3015
Fragments arranged by size:
3015 1156 1002
ApoI r'AATT_y
Cuts at: 1493 2108 2482 2708 2860 4969 1493
Size: 615 374 226 152 2109 1697
Fragments arranged by size:
2109 1697 615 374 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3727 160
Size: 872 2695 1606
Fragments arranged by size:
2695 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5173
AvaI C'yCGr_G
Cuts at: 1870 1943 2097 2498 1870
Size: 73 154 401 4545
Fragments arranged by size:
4545 401 154 73
BamHI G'GATC_C
Cuts at: 2503 2870 2503
Size: 367 4806
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2181 2361 2494 3949 618
Size: 325 217 627 394 180 133 1455 1842
Fragments arranged by size:
1842 1455 627 394 325 217 180 133
BanII G_rGCy'C
Cuts at: 1391 2238 2429 2492 1391
Size: 847 191 63 4072
Fragments arranged by size:
4072 847 191 63
BbeI G_GCGC'C
Cuts at: 2185 2185
Size: 5173
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3364 3398 1903
Size: 34 1427 34 3678
Fragments arranged by size:
3678 1427 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3065 4275 3065
Size: 1210 3963
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5173
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3784 136
Size: 107 122 71 631 605 2112 1525
Fragments arranged by size:
2112 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5168 5168
Size: 5173
BlpI GC'TnA_GC
Cuts at: 2590 2590
Size: 5173
Bme1580I G_kGCm'C
Cuts at: 1790 2232 3234 4390 1790
Size: 442 1002 1156 2573
Fragments arranged by size:
2573 1156 1002 442
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5173
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2904 3862 442
Size: 416 53 83 399 24 1487 958 1753
Fragments arranged by size:
1753 1487 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5173
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 2302 2341 3833 1239
Size: 794 269 39 1492 2579
Fragments arranged by size:
2579 1492 794 269 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3298 4163 4461 3298
Size: 865 298 4010
Fragments arranged by size:
4010 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3836 882
Size: 617 2337 2219
Fragments arranged by size:
2337 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2964 493
Size: 711 1760 2702
Fragments arranged by size:
2702 1760 711
BsaBI GATnn'nnATC
Cuts at: 5167 5167
Size: 5173
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2182 2980 3362 3997
Size: 53 83 186 936 649 798 382 635
Cuts at: 3997 275
Size: 1451
Fragments arranged by size:
1451 936 798 649 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2379 3606 4004 4278 4425 4836 2379
Size: 1227 398 274 147 411 2716
Fragments arranged by size:
2716 1227 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4948
Fragments arranged by size:
4948 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5173
BseYI C'CCAG_C
Cuts at: 2062 2067 2144 4376 4872 2062
Size: 5 77 2232 496 2363
Fragments arranged by size:
2363 2232 496 77 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4600
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2623 3384 4412 1540
Size: 275 186 622 761 1028 2301
Fragments arranged by size:
2301 1028 761 622 275 186
BsiHKAI G_wGCw'C
Cuts at: 2232 3234 3319 4390 2232
Size: 1002 85 1071 3015
Fragments arranged by size:
3015 1071 1002 85
BsmI GAATG_Cn'
Cuts at: 2684 2777 2684
Size: 93 5080
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2232 2238 2429 2492 3234 3319 4390
Size: 399 442 6 191 63 742 85 1071
Cuts at: 4390 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 442 399 191 85 63
6
BspEI T'CCGG_A
Cuts at: 4836 4836
Size: 5173
BspHI T'CATG_A
Cuts at: 3062 4792 3062
Size: 1730 3443
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2288 2528 844
Size: 1444 240 3489
Fragments arranged by size:
3489 1444 240
BsrBI CCG'CTC
Cuts at: 1394 1823 3060 1394
Size: 429 1237 3507
Fragments arranged by size:
3507 1237 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3668 3842 65
Size: 1183 2420 174 1396
Fragments arranged by size:
2420 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3817 4004 1700
Size: 110 2007 187 2869
Fragments arranged by size:
2869 2007 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5173
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5173
BssSI C'ACGA_G
Cuts at: 2231 3233 4245 2231
Size: 1002 1012 3159
Fragments arranged by size:
3159 1012 1002
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5173
BstBI TT'CG_AA
Cuts at: 3977 3977
Size: 5173
BstEII G'GTnAC_C
Cuts at: 1184 2275 1184
Size: 1091 4082
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5173
BstYI r'GATC_y
Cuts at: 1972 2503 2870 3255 3272 4724 4810 4822 5168
Size: 531 367 385 17 1452 86 12 346
Cuts at: 5168 1972
Size: 1977
Fragments arranged by size:
1977 1452 531 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3682
Fragments arranged by size:
3682 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4434
Fragments arranged by size:
4434 683 56
BtsI GCAGTG_nn'
Cuts at: 2689 3483 3503 2689
Size: 794 20 4359
Fragments arranged by size:
4359 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5173
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5138
DraI TTT'AAA
Cuts at: 2477 2477
Size: 5173
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4180 809
Size: 507 2864 1802
Fragments arranged by size:
2864 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2620 3509 5138
Size: 54 1425 50 275 18 790 889 1629
Cuts at: 5138 8
Size: 43
Fragments arranged by size:
1629 1425 889 790 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2620 1537
Size: 275 808 4090
Fragments arranged by size:
4090 808 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3103 1353
Size: 1750 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3760 4134 4280 387
Size: 1064 138 2171 374 146 1280
Fragments arranged by size:
2171 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2302 2341 3236 3833 4620 4888
Size: 393 401 269 39 895 597 787 268
Cuts at: 4888 1239
Size: 1524
Fragments arranged by size:
1524 895 787 597 401 393 269 268
39
EcoNI CCTnn'n_nnAGG
Cuts at: 2087 2087
Size: 5173
EcoRI G'AATT_C
Cuts at: 2482 2860 2482
Size: 378 4795
FspI TGC'GCA
Cuts at: 3679 3679
Size: 5173
HaeII r_GCGC'y
Cuts at: 1678 2185 4320 1678
Size: 507 2135 2531
Fragments arranged by size:
2531 2135 507
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3814 3846 3888 3920 1373
Size: 32 135 32 2242 32 42 32 2626
Fragments arranged by size:
2626 2242 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2517 2763 669
Size: 988 281 579 246 3079
Fragments arranged by size:
3079 988 579 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5173
HpaI GTT'AAC
Cuts at: 2763 2763
Size: 5173
KasI G'GCGC_C
Cuts at: 2181 2181
Size: 5173
KpnI G_GTAC'C
Cuts at: 2365 2498 2365
Size: 133 5040
MfeI C'AATT_G
Cuts at: 2772 2772
Size: 5173
MluI A'CGCG_T
Cuts at: 5049 5049
Size: 5173
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4287 4471 1305
Size: 2982 184 2007
Fragments arranged by size:
2982 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3351
Fragments arranged by size:
3351 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2225 2408 2448 3131 3490 3649 518
Size: 648 1059 183 40 683 359 159 2042
Fragments arranged by size:
2042 1059 683 648 359 183 159 40
MspA1I CmG'CkG
Cuts at: 1606 2116 2589 3266 4414 4659 1606
Size: 510 473 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 510 473 245
NarI GG'CG_CC
Cuts at: 2182 2182
Size: 5173
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3682
Fragments arranged by size:
3682 939 552
NdeI CA'TA_TG
Cuts at: 387 2124 2431 387
Size: 1737 307 3129
Fragments arranged by size:
3129 1737 307
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5173
NotI GC'GGCC_GC
Cuts at: 2620 2620
Size: 5173
NspI r_CATG'y
Cuts at: 1132 1229 2326 2531 4076 1132
Size: 97 1097 205 1545 2229
Fragments arranged by size:
2229 1545 1097 205 97
PasI CC'CwG_GG
Cuts at: 1348 2083 1348
Size: 735 4438
PciI A'CATG_T
Cuts at: 4072 4072
Size: 5173
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5173
PfoI T'CCnGG_A
Cuts at: 2265 2265
Size: 5173
PmeI GTTT'AAAC
Cuts at: 2477 2477
Size: 5173
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3242 3274 4755 4787 3242
Size: 32 1481 32 3628
Fragments arranged by size:
3628 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5173
PsiI TTA'TAA
Cuts at: 2743 2743
Size: 5173
PstI C_TGCA'G
Cuts at: 830 2525 830
Size: 1695 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5173
SalI G'TCGA_C
Cuts at: 1936 2515 1936
Size: 579 4594
SbfI CC_TGCA'GG
Cuts at: 2525 2525
Size: 5173
ScaI AGT'ACT
Cuts at: 3421 5133 3421
Size: 1712 3461
SfcI C'TryA_G
Cuts at: 826 1045 2521 3656 4337 4528 826
Size: 219 1476 1135 681 191 1471
Fragments arranged by size:
1476 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2183 2183
Size: 5173
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5173
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5173
SmaI CCC'GGG
Cuts at: 1872 2099 2500 1872
Size: 227 401 4545
Fragments arranged by size:
4545 401 227
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3277 4178 4440 820
Size: 197 926 1334 901 262 1553
Fragments arranged by size:
1553 1334 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2964 493
Size: 2471 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5173
SphI G_CATG'C
Cuts at: 1132 2326 2531 1132
Size: 1194 205 3774
Fragments arranged by size:
3774 1194 205
SspI AAT'ATT
Cuts at: 5 52 3097 5
Size: 47 3045 2081
Fragments arranged by size:
3045 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5173
StyI C'CwwG_G
Cuts at: 513 1065 2004 2612 513
Size: 552 939 608 3074
Fragments arranged by size:
3074 939 608 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3200 3217 3370 3555 4000 325
Size: 1255 1620 17 153 185 445 1498
Fragments arranged by size:
1620 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3419 5131 96
Size: 274 80 33 51 1443 1442 1712 138
Fragments arranged by size:
1712 1443 1442 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5133 5165 5133
Size: 32 5141
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5173
XbaI T'CTAG_A
Cuts at: 2509 2509
Size: 5173
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5173
XmaI C'CCGG_G
Cuts at: 1870 2097 2498 1870
Size: 227 401 4545
Fragments arranged by size:
4545 401 227
XmnI GAAnn'nnTTC
Cuts at: 3302 3302
Size: 5173
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2981 276
Size: 53 83 186 936 1447 2468
Fragments arranged by size:
2468 1447 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI