Restriction map for
pFN21AE5165
(Circular) (Six-base) MAPSORT of: pFN21AE5165.seq Check: 3486 from: 1 to: 5125
pFN21AE5165 5125 bp
With 182 enzymes: SgfI *
January 30, 2014 17:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2284 2284
Size: 5125
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2935 278
Size: 53 83 186 936 1399 2468
Fragments arranged by size:
2468 1399 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2468 1937
Size: 531 4594
Acc65I G'GTAC_C
Cuts at: 2446 2446
Size: 5125
AclI AA'CG_TT
Cuts at: 1563 3252 3625 1563
Size: 1689 373 3063
Fragments arranged by size:
3063 1689 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2392 3188 4572 4840 1632
Size: 760 796 1384 268 1917
Fragments arranged by size:
1917 1384 796 760 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4928
AflIII A'CryG_T
Cuts at: 1203 2196 4024 5001 1203
Size: 993 1828 977 1327
Fragments arranged by size:
1828 1327 993 977
AgeI A'CCGG_T
Cuts at: 3956 3956
Size: 5125
AhdI GACnn_n'nnGTC
Cuts at: 3854 3854
Size: 5125
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5125
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2316 1184
Size: 1132 3993
AlwNI CAG_nnn'CTG
Cuts at: 2167 2868 4440 2167
Size: 701 1572 2852
Fragments arranged by size:
2852 1572 701
ApaI G_GGCC'C
Cuts at: 2185 2185
Size: 5125
ApaLI G'TGCA_C
Cuts at: 3182 4338 3182
Size: 1156 3969
ApoI r'AATT_y
Cuts at: 1493 2434 2660 2812 4921 1493
Size: 941 226 152 2109 1697
Fragments arranged by size:
2109 1697 941 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3679 160
Size: 872 2647 1606
Fragments arranged by size:
2647 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5125
AvaI C'yCGr_G
Cuts at: 1870 1943 2184 2450 1870
Size: 73 241 266 4545
Fragments arranged by size:
4545 266 241 73
BamHI G'GATC_C
Cuts at: 2050 2373 2455 2822 2050
Size: 323 82 367 4353
Fragments arranged by size:
4353 367 323 82
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2446 3901 618
Size: 325 217 627 659 1455 1842
Fragments arranged by size:
1842 1455 659 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2025 2185 2444 1391
Size: 634 160 259 4072
Fragments arranged by size:
4072 634 259 160
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2135 2398 928
Size: 971 236 263 3655
Fragments arranged by size:
3655 971 263 236
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3316 3350 1903
Size: 34 1379 34 3678
Fragments arranged by size:
3678 1379 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3017 4227 3017
Size: 1210 3915
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5125
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2150 3736 136
Size: 107 122 71 631 605 478 1586 1525
Fragments arranged by size:
1586 1525 631 605 478 122 107 71
BglII A'GATC_T
Cuts at: 2208 5120 2208
Size: 2912 2213
BlpI GC'TnA_GC
Cuts at: 2542 2542
Size: 5125
Bme1580I G_kGCm'C
Cuts at: 1790 2185 3186 4342 1790
Size: 395 1001 1156 2573
Fragments arranged by size:
2573 1156 1001 395
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5125
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2856 3814 442
Size: 416 53 83 399 24 1439 958 1753
Fragments arranged by size:
1753 1439 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5125
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2357 3785 1239
Size: 1118 1428 2579
Fragments arranged by size:
2579 1428 1118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3250 4115 4413 3250
Size: 865 298 3962
Fragments arranged by size:
3962 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2095 2339 3788 882
Size: 617 596 244 1449 2219
Fragments arranged by size:
2219 1449 617 596 244
BsaAI yAC'GTr
Cuts at: 493 1204 2916 493
Size: 711 1712 2702
Fragments arranged by size:
2702 1712 711
BsaBI GATnn'nnATC
Cuts at: 5119 5119
Size: 5125
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2932 3314 3949 275
Size: 53 83 186 936 1399 382 635 1451
Fragments arranged by size:
1451 1399 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3558 3956 4230 4377 4788 3558
Size: 398 274 147 411 3895
Fragments arranged by size:
3895 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4900
Fragments arranged by size:
4900 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5125
BseYI C'CCAG_C
Cuts at: 2084 2145 4328 4824 2084
Size: 61 2183 496 2385
Fragments arranged by size:
2385 2183 496 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4552
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2575 3336 4364 1540
Size: 275 186 574 761 1028 2301
Fragments arranged by size:
2301 1028 761 574 275 186
BsiHKAI G_wGCw'C
Cuts at: 2204 3186 3271 4342 2204
Size: 982 85 1071 2987
Fragments arranged by size:
2987 1071 982 85
BsmI GAATG_Cn'
Cuts at: 2636 2729 2636
Size: 93 5032
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2185 2204 2444 3186 3271 4342
Size: 399 235 160 19 240 742 85 1071
Cuts at: 4342 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 240 235 160 85
19
BspEI T'CCGG_A
Cuts at: 4788 4788
Size: 5125
BspHI T'CATG_A
Cuts at: 3014 4744 3014
Size: 1730 3395
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2284 2480 844
Size: 1440 196 3489
Fragments arranged by size:
3489 1440 196
BsrBI CCG'CTC
Cuts at: 1394 1823 3012 1394
Size: 429 1189 3507
Fragments arranged by size:
3507 1189 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3620 3794 65
Size: 1183 2372 174 1396
Fragments arranged by size:
2372 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3769 3956 1700
Size: 110 1959 187 2869
Fragments arranged by size:
2869 1959 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5125
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5125
BssSI C'ACGA_G
Cuts at: 3185 4197 3185
Size: 1012 4113
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5125
BstBI TT'CG_AA
Cuts at: 3929 3929
Size: 5125
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5125
BstYI r'GATC_y
Cuts at: 1972 2050 2208 2373 2455 2822 3207 3224 4676
Size: 78 158 165 82 367 385 17 1452
Cuts at: 4676 4762 4774 5120 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 165 158 86
82 78 17 12
Bsu36I CC'TnA_GG
Cuts at: 2069 2069
Size: 5125
BtgI C'CryG_G
Cuts at: 513 1065 2004 2026 2316 513
Size: 552 939 22 290 3322
Fragments arranged by size:
3322 939 552 290 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2239 487
Size: 683 56 1013 3373
Fragments arranged by size:
3373 1013 683 56
BtsI GCAGTG_nn'
Cuts at: 2641 3435 3455 2641
Size: 794 20 4311
Fragments arranged by size:
4311 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5125
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5090
DraI TTT'AAA
Cuts at: 2429 2429
Size: 5125
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2117 4132 809
Size: 507 801 2015 1802
Fragments arranged by size:
2015 1802 801 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 2572 3461
Size: 54 1425 50 275 18 402 340 889
Cuts at: 3461 5090 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 402 340 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2572 1537
Size: 275 760 4090
Fragments arranged by size:
4090 760 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3055 1353
Size: 1702 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3712 4086 4232 387
Size: 1064 138 2123 374 146 1280
Fragments arranged by size:
2123 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2357 2392 3188 3785 4572 4840 1239
Size: 393 725 35 796 597 787 268 1524
Fragments arranged by size:
1524 796 787 725 597 393 268 35
EcoO109I rG'GnC_Cy
Cuts at: 2181 2286 2181
Size: 105 5020
EcoRI G'AATT_C
Cuts at: 2434 2812 2434
Size: 378 4747
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2152 2152
Size: 5125
FspI TGC'GCA
Cuts at: 3631 3631
Size: 5125
HaeII r_GCGC'y
Cuts at: 1678 4272 1678
Size: 2594 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3766 3798 3840 3872 1373
Size: 32 135 32 2194 32 42 32 2626
Fragments arranged by size:
2626 2194 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2122 2469 2715 669
Size: 988 281 184 347 246 3079
Fragments arranged by size:
3079 988 347 281 246 184
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5125
HpaI GTT'AAC
Cuts at: 2715 2715
Size: 5125
KpnI G_GTAC'C
Cuts at: 2450 2450
Size: 5125
MfeI C'AATT_G
Cuts at: 2724 2724
Size: 5125
MluI A'CGCG_T
Cuts at: 2196 5001 2196
Size: 2805 2320
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4239 4423 1305
Size: 2934 184 2007
Fragments arranged by size:
2934 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2234 10
Size: 54 1425 343 402 2901
Fragments arranged by size:
2901 1425 402 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3083 3442 3601 518
Size: 648 1917 359 159 2042
Fragments arranged by size:
2042 1917 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2028 2149 2157 2266 2541 3218 4366 4611
Size: 422 121 8 109 275 677 1148 245
Cuts at: 4611 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 422 275 245 121 109
8
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3634
Fragments arranged by size:
3634 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5125
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5125
NotI GC'GGCC_GC
Cuts at: 2572 2572
Size: 5125
NspI r_CATG'y
Cuts at: 1132 1229 2483 4028 1132
Size: 97 1254 1545 2229
Fragments arranged by size:
2229 1545 1254 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5125
PciI A'CATG_T
Cuts at: 4024 4024
Size: 5125
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5125
PmeI GTTT'AAAC
Cuts at: 2429 2429
Size: 5125
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2186 2218 3194 3226 4707 4739 2186
Size: 32 976 32 1481 32 2572
Fragments arranged by size:
2572 1481 976 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2286 2286
Size: 5125
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5125
PsiI TTA'TAA
Cuts at: 2695 2695
Size: 5125
PspOMI G'GGCC_C
Cuts at: 2181 2181
Size: 5125
PstI C_TGCA'G
Cuts at: 830 2265 2271 2477 830
Size: 1435 6 206 3478
Fragments arranged by size:
3478 1435 206 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5125
PvuII CAG'CTG
Cuts at: 2149 2266 2149
Size: 117 5008
SacII CC_GC'GG
Cuts at: 2029 2029
Size: 5125
SalI G'TCGA_C
Cuts at: 1936 2467 1936
Size: 531 4594
SbfI CC_TGCA'GG
Cuts at: 2477 2477
Size: 5125
ScaI AGT'ACT
Cuts at: 3373 5085 3373
Size: 1712 3413
SfcI C'TryA_G
Cuts at: 826 1045 2261 2267 2473 3608 4289 4480 826
Size: 219 1216 6 206 1135 681 191 1471
Fragments arranged by size:
1471 1216 1135 681 219 206 191 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 2150 2150
Size: 5125
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5125
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5125
SmaI CCC'GGG
Cuts at: 1872 2186 2452 1872
Size: 314 266 4545
Fragments arranged by size:
4545 314 266
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3229 4130 4392 820
Size: 197 926 1286 901 262 1553
Fragments arranged by size:
1553 1286 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2916 493
Size: 2423 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5125
SphI G_CATG'C
Cuts at: 1132 2483 1132
Size: 1351 3774
SspI AAT'ATT
Cuts at: 5 52 3049 5
Size: 47 2997 2081
Fragments arranged by size:
2997 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5125
StyI C'CwwG_G
Cuts at: 513 1065 2004 2421 2564 513
Size: 552 939 417 143 3074
Fragments arranged by size:
3074 939 552 417 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2363 2434 3152 3169 3322 3507 3952
Size: 1255 783 71 718 17 153 185 445
Cuts at: 3952 325
Size: 1498
Fragments arranged by size:
1498 1255 783 718 445 185 153 71
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3371 5083 96
Size: 274 80 33 51 1443 1394 1712 138
Fragments arranged by size:
1712 1443 1394 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5085 5117 5085
Size: 32 5093
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5125
XbaI T'CTAG_A
Cuts at: 2461 2461
Size: 5125
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5125
XmaI C'CCGG_G
Cuts at: 1870 2184 2450 1870
Size: 314 266 4545
Fragments arranged by size:
4545 314 266
XmnI GAAnn'nnTTC
Cuts at: 3254 3254
Size: 5125
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2933 276
Size: 53 83 186 936 1399 2468
Fragments arranged by size:
2468 1399 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacI SanDI SapI SexAI SfoI SrfI SwaI XcmI