Restriction map for
pFN21AE5161
(Circular) (Six-base) MAPSORT of: pFN21AE5161.seq Check: 908 from: 1 to: 5107
pFN21AE5161 5107 bp
With 182 enzymes: SgfI *
January 30, 2014 17:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2167 2167
Size: 5107
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2917 278
Size: 53 83 186 936 1381 2468
Fragments arranged by size:
2468 1381 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2450 1937
Size: 513 4594
Acc65I G'GTAC_C
Cuts at: 2428 2428
Size: 5107
AclI AA'CG_TT
Cuts at: 1563 3234 3607 1563
Size: 1671 373 3063
Fragments arranged by size:
3063 1671 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3170 4554 4822 1632
Size: 1538 1384 268 1917
Fragments arranged by size:
1917 1538 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4910
AflIII A'CryG_T
Cuts at: 1203 4006 4983 1203
Size: 2803 977 1327
Fragments arranged by size:
2803 1327 977
AgeI A'CCGG_T
Cuts at: 3938 3938
Size: 5107
AhdI GACnn_n'nnGTC
Cuts at: 3836 3836
Size: 5107
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5107
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2168 1184
Size: 984 4123
AlwNI CAG_nnn'CTG
Cuts at: 2850 4422 2850
Size: 1572 3535
ApaLI G'TGCA_C
Cuts at: 3164 4320 3164
Size: 1156 3951
ApoI r'AATT_y
Cuts at: 1493 2416 2642 2794 4903 1493
Size: 923 226 152 2109 1697
Fragments arranged by size:
2109 1697 923 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3661 160
Size: 872 2629 1606
Fragments arranged by size:
2629 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5107
AvaI C'yCGr_G
Cuts at: 1870 1943 2028 2241 2432 1870
Size: 73 85 213 191 4545
Fragments arranged by size:
4545 213 191 85 73
BamHI G'GATC_C
Cuts at: 2008 2437 2804 2008
Size: 429 367 4311
Fragments arranged by size:
4311 429 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2271 2302 2428 3883 618
Size: 325 217 627 484 31 126 1455 1842
Fragments arranged by size:
1842 1455 627 484 325 217 126 31
BanII G_rGCy'C
Cuts at: 1391 2202 2216 2426 1391
Size: 811 14 210 4072
Fragments arranged by size:
4072 811 210 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4136
BbvCI CC'TCA_GC
Cuts at: 2396 2396
Size: 5107
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3298 3332 1903
Size: 34 1361 34 3678
Fragments arranged by size:
3678 1361 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2999 4209 2999
Size: 1210 3897
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5107
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3718 136
Size: 107 122 71 631 605 2046 1525
Fragments arranged by size:
2046 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5102 5102
Size: 5107
BlpI GC'TnA_GC
Cuts at: 2524 2524
Size: 5107
Bme1580I G_kGCm'C
Cuts at: 1790 2274 2305 3168 4324 1790
Size: 484 31 863 1156 2573
Fragments arranged by size:
2573 1156 863 484 31
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5107
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2838 3796 442
Size: 416 53 83 399 24 1421 958 1753
Fragments arranged by size:
1753 1421 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5107
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 2216 2356 3767 1239
Size: 950 27 140 1411 2579
Fragments arranged by size:
2579 1411 950 140 27
Bpu10I CC'TnA_GC
Cuts at: 2396 2396
Size: 5107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3232 4097 4395 3232
Size: 865 298 3944
Fragments arranged by size:
3944 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3770 882
Size: 617 2271 2219
Fragments arranged by size:
2271 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2898 493
Size: 711 1694 2702
Fragments arranged by size:
2702 1694 711
BsaBI GATnn'nnATC
Cuts at: 5101 5101
Size: 5107
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2914 3296 3931 275
Size: 53 83 186 936 1381 382 635 1451
Fragments arranged by size:
1451 1381 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2358 3540 3938 4212 4359 4770 2358
Size: 1182 398 274 147 411 2695
Fragments arranged by size:
2695 1182 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4882
Fragments arranged by size:
4882 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2359 1186
Size: 1173 3934
BseYI C'CCAG_C
Cuts at: 2089 4310 4806 2089
Size: 2221 496 2390
Fragments arranged by size:
2390 2221 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4534
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2557 3318 4346 1540
Size: 275 186 556 761 1028 2301
Fragments arranged by size:
2301 1028 761 556 275 186
BsiHKAI G_wGCw'C
Cuts at: 3168 3253 4324 3168
Size: 85 1071 3951
Fragments arranged by size:
3951 1071 85
BsmI GAATG_Cn'
Cuts at: 2618 2711 2618
Size: 93 5014
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2216 2274 2305 2426 3168 3253
Size: 399 412 14 58 31 121 742 85
Cuts at: 3253 4324 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 412 399 121 85 58
31 14
BspEI T'CCGG_A
Cuts at: 4770 4770
Size: 5107
BspHI T'CATG_A
Cuts at: 2996 4726 2996
Size: 1730 3377
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2167 2462 844
Size: 1323 295 3489
Fragments arranged by size:
3489 1323 295
BsrBI CCG'CTC
Cuts at: 1394 1823 2994 1394
Size: 429 1171 3507
Fragments arranged by size:
3507 1171 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3602 3776 65
Size: 1183 2354 174 1396
Fragments arranged by size:
2354 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3751 3938 1700
Size: 110 1941 187 2869
Fragments arranged by size:
2869 1941 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5107
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5107
BssSI C'ACGA_G
Cuts at: 3167 4179 3167
Size: 1012 4095
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5107
BstBI TT'CG_AA
Cuts at: 3911 3911
Size: 5107
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5107
BstXI CCAn_nnnn'nTGG
Cuts at: 2380 2380
Size: 5107
BstYI r'GATC_y
Cuts at: 1972 2008 2437 2804 3189 3206 4658 4744 4756
Size: 36 429 367 385 17 1452 86 12
Cuts at: 4756 5102 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 429 385 367 346 86 36
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2266 513
Size: 552 939 262 3354
Fragments arranged by size:
3354 939 552 262
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4368
Fragments arranged by size:
4368 683 56
BtsI GCAGTG_nn'
Cuts at: 2623 3417 3437 2623
Size: 794 20 4293
Fragments arranged by size:
4293 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5107
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5072
DraI TTT'AAA
Cuts at: 2411 2411
Size: 5107
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4114 809
Size: 507 2798 1802
Fragments arranged by size:
2798 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2554 3443 5072
Size: 54 1425 50 275 18 724 889 1629
Cuts at: 5072 8
Size: 43
Fragments arranged by size:
1629 1425 889 724 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2554 1537
Size: 275 742 4090
Fragments arranged by size:
4090 742 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 3037 1353
Size: 701 983 3423
Fragments arranged by size:
3423 983 701
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3694 4068 4214 387
Size: 1064 138 2105 374 146 1280
Fragments arranged by size:
2105 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2216 2356 3170 3767 4554 4822
Size: 393 557 27 140 814 597 787 268
Cuts at: 4822 1239
Size: 1524
Fragments arranged by size:
1524 814 787 597 557 393 268 140
27
EcoRI G'AATT_C
Cuts at: 2416 2794 2416
Size: 378 4729
FspI TGC'GCA
Cuts at: 3613 3613
Size: 5107
HaeII r_GCGC'y
Cuts at: 1678 2036 4254 1678
Size: 358 2218 2531
Fragments arranged by size:
2531 2218 358
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2174 2203 2206 2235 3748
Size: 32 135 32 602 29 3 29 1513
Cuts at: 3748 3780 3822 3854 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1513 602 135 42 32 32 32
32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2451 2697 669
Size: 988 281 513 246 3079
Fragments arranged by size:
3079 988 513 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5107
HpaI GTT'AAC
Cuts at: 2697 2697
Size: 5107
KpnI G_GTAC'C
Cuts at: 2432 2432
Size: 5107
MfeI C'AATT_G
Cuts at: 2706 2706
Size: 5107
MluI A'CGCG_T
Cuts at: 4983 4983
Size: 5107
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2148 4221 4405 1305
Size: 843 2073 184 2007
Fragments arranged by size:
2073 2007 843 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3285
Fragments arranged by size:
3285 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3065 3424 3583 518
Size: 648 1899 359 159 2042
Fragments arranged by size:
2042 1899 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2089 2523 3200 4348 4593 1606
Size: 483 434 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 483 434 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3616
Fragments arranged by size:
3616 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5107
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5107
NotI GC'GGCC_GC
Cuts at: 2554 2554
Size: 5107
NspI r_CATG'y
Cuts at: 1132 1229 2408 2465 4010 1132
Size: 97 1179 57 1545 2229
Fragments arranged by size:
2229 1545 1179 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5107
PciI A'CATG_T
Cuts at: 4006 4006
Size: 5107
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2089 1108
Size: 981 4126
PfoI T'CCnGG_A
Cuts at: 2205 2205
Size: 5107
PmeI GTTT'AAAC
Cuts at: 2411 2411
Size: 5107
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3176 3208 4689 4721 3176
Size: 32 1481 32 3562
Fragments arranged by size:
3562 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5107
PsiI TTA'TAA
Cuts at: 2677 2677
Size: 5107
PstI C_TGCA'G
Cuts at: 830 2178 2459 830
Size: 1348 281 3478
Fragments arranged by size:
3478 1348 281
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5107
SalI G'TCGA_C
Cuts at: 1936 2449 1936
Size: 513 4594
SapI GCTCTTCn'nnn_
Cuts at: 2054 2054
Size: 5107
SbfI CC_TGCA'GG
Cuts at: 2459 2459
Size: 5107
ScaI AGT'ACT
Cuts at: 3355 5067 3355
Size: 1712 3395
SfcI C'TryA_G
Cuts at: 826 1045 2174 2455 3590 4271 4462 826
Size: 219 1129 281 1135 681 191 1471
Fragments arranged by size:
1471 1135 1129 681 281 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5107
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5107
SmaI CCC'GGG
Cuts at: 1872 2030 2243 2434 1872
Size: 158 213 191 4545
Fragments arranged by size:
4545 213 191 158
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3211 4112 4374 820
Size: 197 926 1268 901 262 1553
Fragments arranged by size:
1553 1268 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2898 493
Size: 2405 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5107
SphI G_CATG'C
Cuts at: 1132 2465 1132
Size: 1333 3774
SspI AAT'ATT
Cuts at: 5 52 3031 5
Size: 47 2979 2081
Fragments arranged by size:
2979 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5107
StyI C'CwwG_G
Cuts at: 513 1065 2004 2546 513
Size: 552 939 542 3074
Fragments arranged by size:
3074 939 552 542
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3134 3151 3304 3489 3934 325
Size: 1255 1554 17 153 185 445 1498
Fragments arranged by size:
1554 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3353 5065 96
Size: 274 80 33 51 1443 1376 1712 138
Fragments arranged by size:
1712 1443 1376 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5067 5099 5067
Size: 32 5075
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5107
XbaI T'CTAG_A
Cuts at: 2443 2443
Size: 5107
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5107
XmaI C'CCGG_G
Cuts at: 1870 2028 2241 2432 1870
Size: 158 213 191 4545
Fragments arranged by size:
4545 213 191 158
XmnI GAAnn'nnTTC
Cuts at: 3236 3236
Size: 5107
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2915 276
Size: 53 83 186 936 1381 2468
Fragments arranged by size:
2468 1381 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI