Restriction map for
pFN21AE5158
(Circular) (Six-base) MAPSORT of: pFN21AE5158.seq Check: 5825 from: 1 to: 5149
pFN21AE5158 5149 bp
With 182 enzymes: SgfI *
January 30, 2014 17:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2959 278
Size: 53 83 186 936 1423 2468
Fragments arranged by size:
2468 1423 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2492 1937
Size: 555 4594
Acc65I G'GTAC_C
Cuts at: 2470 2470
Size: 5149
AclI AA'CG_TT
Cuts at: 1563 3276 3649 1563
Size: 1713 373 3063
Fragments arranged by size:
3063 1713 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2374 3212 4596 4864 1632
Size: 742 838 1384 268 1917
Fragments arranged by size:
1917 1384 838 742 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4952
AflIII A'CryG_T
Cuts at: 1203 4048 5025 1203
Size: 2845 977 1327
Fragments arranged by size:
2845 1327 977
AgeI A'CCGG_T
Cuts at: 3980 3980
Size: 5149
AhdI GACnn_n'nnGTC
Cuts at: 3878 3878
Size: 5149
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5149
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5149
AlwNI CAG_nnn'CTG
Cuts at: 2892 4464 2892
Size: 1572 3577
ApaLI G'TGCA_C
Cuts at: 3206 4362 3206
Size: 1156 3993
ApoI r'AATT_y
Cuts at: 1493 2384 2458 2684 2836 4945 1493
Size: 891 74 226 152 2109 1697
Fragments arranged by size:
2109 1697 891 226 152 74
AseI AT'TA_AT
Cuts at: 160 1032 3703 160
Size: 872 2671 1606
Fragments arranged by size:
2671 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5149
AvaI C'yCGr_G
Cuts at: 1870 1943 2474 1870
Size: 73 531 4545
Fragments arranged by size:
4545 531 73
BamHI G'GATC_C
Cuts at: 2479 2846 2479
Size: 367 4782
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2470 3925 618
Size: 325 217 627 683 1455 1842
Fragments arranged by size:
1842 1455 683 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2468 1391
Size: 1077 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2030 928
Size: 971 131 4047
Fragments arranged by size:
4047 971 131
BbvCI CC'TCA_GC
Cuts at: 2403 2403
Size: 5149
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3340 3374 1903
Size: 34 50 34 1319 34 3678
Fragments arranged by size:
3678 1319 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3041 4251 3041
Size: 1210 3939
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5149
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2070 3760 136
Size: 107 122 71 631 605 398 1690 1525
Fragments arranged by size:
1690 1525 631 605 398 122 107 71
BglII A'GATC_T
Cuts at: 5144 5144
Size: 5149
BlpI GC'TnA_GC
Cuts at: 2566 2566
Size: 5149
Bme1580I G_kGCm'C
Cuts at: 1790 3210 4366 1790
Size: 1420 1156 2573
Fragments arranged by size:
2573 1420 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5149
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2298 2880 3838
Size: 416 53 83 399 24 881 582 958
Cuts at: 3838 442
Size: 1753
Fragments arranged by size:
1753 958 881 582 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5149
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3809 1239
Size: 2570 2579
Bpu10I CC'TnA_GC
Cuts at: 2403 2403
Size: 5149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3274 4139 4437 3274
Size: 865 298 3986
Fragments arranged by size:
3986 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3812 882
Size: 617 2313 2219
Fragments arranged by size:
2313 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2047 2940 493
Size: 711 843 893 2702
Fragments arranged by size:
2702 893 843 711
BsaBI GATnn'nnATC
Cuts at: 5143 5143
Size: 5149
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2956 3338 3973 275
Size: 53 83 186 936 1423 382 635 1451
Fragments arranged by size:
1451 1423 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2079 2127 3582 3980 4254 4401 4812 2079
Size: 48 1455 398 274 147 411 2416
Fragments arranged by size:
2416 1455 411 398 274 147 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4924
Fragments arranged by size:
4924 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2420 1186
Size: 1234 3915
BseYI C'CCAG_C
Cuts at: 4352 4848 4352
Size: 496 4653
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4576
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2065 2098 2321 2599 3360 4388
Size: 275 186 64 33 223 278 761 1028
Cuts at: 4388 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 278 275 223 186 64
33
BsiHKAI G_wGCw'C
Cuts at: 3210 3295 4366 3210
Size: 85 1071 3993
Fragments arranged by size:
3993 1071 85
BsmI GAATG_Cn'
Cuts at: 2275 2660 2753 2275
Size: 385 93 4671
Fragments arranged by size:
4671 385 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2468 3210 3295 4366 1391
Size: 399 678 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 678 399 85
BspEI T'CCGG_A
Cuts at: 4812 4812
Size: 5149
BspHI T'CATG_A
Cuts at: 3038 4768 3038
Size: 1730 3419
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2504 844
Size: 1660 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3036 1394
Size: 429 1213 3507
Fragments arranged by size:
3507 1213 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3644 3818 65
Size: 1183 2396 174 1396
Fragments arranged by size:
2396 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3793 3980 1700
Size: 110 1983 187 2869
Fragments arranged by size:
2869 1983 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5149
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5149
BssSI C'ACGA_G
Cuts at: 3209 4221 3209
Size: 1012 4137
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5149
BstBI TT'CG_AA
Cuts at: 3953 3953
Size: 5149
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5149
BstYI r'GATC_y
Cuts at: 1972 2479 2846 3231 3248 4700 4786 4798 5144
Size: 507 367 385 17 1452 86 12 346
Cuts at: 5144 1972
Size: 1977
Fragments arranged by size:
1977 1452 507 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2053 2059 2098 513
Size: 552 988 6 39 3564
Fragments arranged by size:
3564 988 552 39 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4410
Fragments arranged by size:
4410 683 56
BtsI GCAGTG_nn'
Cuts at: 2665 3459 3479 2665
Size: 794 20 4335
Fragments arranged by size:
4335 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5149
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5114
DraI TTT'AAA
Cuts at: 2453 2453
Size: 5149
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4156 809
Size: 507 2840 1802
Fragments arranged by size:
2840 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2056 2062 2095
Size: 54 1425 50 275 18 226 6 33
Cuts at: 2095 2596 3485 5114 8
Size: 501 889 1629 43
Fragments arranged by size:
1629 1425 889 501 275 226 54 50
43 33 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2062 2095 2596 1537
Size: 275 250 33 501 4090
Fragments arranged by size:
4090 501 275 250 33
EarI CTCTTCn'nnn_
Cuts at: 1353 2392 3079 1353
Size: 1039 687 3423
Fragments arranged by size:
3423 1039 687
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3736 4110 4256 387
Size: 1064 138 2147 374 146 1280
Fragments arranged by size:
2147 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2374 3212 3809 4596 4864 1239
Size: 393 742 838 597 787 268 1524
Fragments arranged by size:
1524 838 787 742 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2430 2430
Size: 5149
EcoRI G'AATT_C
Cuts at: 2458 2836 2458
Size: 378 4771
FspI TGC'GCA
Cuts at: 3655 3655
Size: 5149
HaeII r_GCGC'y
Cuts at: 1678 4296 1678
Size: 2618 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3790 3822 3864 3896 1373
Size: 32 135 32 2218 32 42 32 2626
Fragments arranged by size:
2626 2218 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2224 2493 2739 669
Size: 988 281 286 269 246 3079
Fragments arranged by size:
3079 988 286 281 269 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5149
HpaI GTT'AAC
Cuts at: 2739 2739
Size: 5149
KpnI G_GTAC'C
Cuts at: 2474 2474
Size: 5149
MfeI C'AATT_G
Cuts at: 2748 2748
Size: 5149
MluI A'CGCG_T
Cuts at: 5025 5025
Size: 5149
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4263 4447 1305
Size: 2958 184 2007
Fragments arranged by size:
2958 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3327
Fragments arranged by size:
3327 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2309 3107 3466 3625 518
Size: 648 1143 798 359 159 2042
Fragments arranged by size:
2042 1143 798 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2100 2565 3242 4390 4635 1606
Size: 494 465 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 494 465 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4597
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5149
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5149
NotI GC'GGCC_GC
Cuts at: 2095 2596 2095
Size: 501 4648
NspI r_CATG'y
Cuts at: 1132 1229 2507 4052 1132
Size: 97 1278 1545 2229
Fragments arranged by size:
2229 1545 1278 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5149
PciI A'CATG_T
Cuts at: 4048 4048
Size: 5149
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5149
PmeI GTTT'AAAC
Cuts at: 2453 2453
Size: 5149
PmlI CAC'GTG
Cuts at: 2047 2047
Size: 5149
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3218 3250 4731 4763 3218
Size: 32 1481 32 3604
Fragments arranged by size:
3604 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2246 1613
Size: 633 4516
PsiI TTA'TAA
Cuts at: 2719 2719
Size: 5149
PstI C_TGCA'G
Cuts at: 830 2501 830
Size: 1671 3478
PvuI CG_AT'CG
Cuts at: 2001 2321 2001
Size: 320 4829
SacII CC_GC'GG
Cuts at: 2101 2101
Size: 5149
SalI G'TCGA_C
Cuts at: 1936 2491 1936
Size: 555 4594
SapI GCTCTTCn'nnn_
Cuts at: 2392 2392
Size: 5149
SbfI CC_TGCA'GG
Cuts at: 2501 2501
Size: 5149
ScaI AGT'ACT
Cuts at: 3397 5109 3397
Size: 1712 3437
SfcI C'TryA_G
Cuts at: 826 1045 2497 3632 4313 4504 826
Size: 219 1452 1135 681 191 1471
Fragments arranged by size:
1471 1452 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5149
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5149
SmaI CCC'GGG
Cuts at: 1872 2476 1872
Size: 604 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3253 4154 4416 820
Size: 197 926 1310 901 262 1553
Fragments arranged by size:
1553 1310 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2940 493
Size: 2447 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5149
SphI G_CATG'C
Cuts at: 1132 2507 1132
Size: 1375 3774
SspI AAT'ATT
Cuts at: 5 52 3073 5
Size: 47 3021 2081
Fragments arranged by size:
3021 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5149
StyI C'CwwG_G
Cuts at: 513 1065 2588 513
Size: 552 1523 3074
Fragments arranged by size:
3074 1523 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2166 3176 3193 3346 3531 3976 325
Size: 1255 586 1010 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1010 586 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3395 5107 96
Size: 274 80 33 51 1443 1418 1712 138
Fragments arranged by size:
1712 1443 1418 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5109 5141 5109
Size: 32 5117
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5149
XbaI T'CTAG_A
Cuts at: 2485 2485
Size: 5149
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2308 2308
Size: 5149
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5149
XmaI C'CCGG_G
Cuts at: 1870 2474 1870
Size: 604 4545
XmnI GAAnn'nnTTC
Cuts at: 3278 3278
Size: 5149
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2957 276
Size: 53 83 186 936 1423 2468
Fragments arranged by size:
2468 1423 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PstI PvuI SacII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI
SfoI SrfI SwaI