Restriction map for
pFN21AE5154
(Circular) (Six-base) MAPSORT of: pFN21AE5154.seq Check: 4571 from: 1 to: 5005
pFN21AE5154 5005 bp
With 182 enzymes: SgfI *
January 30, 2014 17:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2086 2086
Size: 5005
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2815 278
Size: 53 83 186 936 1279 2468
Fragments arranged by size:
2468 1279 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2348 1937
Size: 411 4594
Acc65I G'GTAC_C
Cuts at: 2109 2326 2109
Size: 217 4788
AclI AA'CG_TT
Cuts at: 1563 3132 3505 1563
Size: 1569 373 3063
Fragments arranged by size:
3063 1569 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3068 4452 4720 1632
Size: 1436 1384 268 1917
Fragments arranged by size:
1917 1436 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4808
AflIII A'CryG_T
Cuts at: 1203 3904 4881 1203
Size: 2701 977 1327
Fragments arranged by size:
2701 1327 977
AgeI A'CCGG_T
Cuts at: 3836 3836
Size: 5005
AhdI GACnn_n'nnGTC
Cuts at: 3734 3734
Size: 5005
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5005
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5005
AlwNI CAG_nnn'CTG
Cuts at: 2748 4320 2748
Size: 1572 3433
ApaLI G'TGCA_C
Cuts at: 3062 4218 3062
Size: 1156 3849
ApoI r'AATT_y
Cuts at: 1493 2314 2540 2692 4801 1493
Size: 821 226 152 2109 1697
Fragments arranged by size:
2109 1697 821 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3559 160
Size: 872 2527 1606
Fragments arranged by size:
2527 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5005
AvaI C'yCGr_G
Cuts at: 1870 1943 2330 1870
Size: 73 387 4545
Fragments arranged by size:
4545 387 73
BamHI G'GATC_C
Cuts at: 2335 2702 2335
Size: 367 4638
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2109 2269 2326 3781 618
Size: 325 217 627 322 160 57 1455 1842
Fragments arranged by size:
1842 1455 627 325 322 217 160 57
BanII G_rGCy'C
Cuts at: 1391 2283 2324 1391
Size: 892 41 4072
Fragments arranged by size:
4072 892 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2108 928
Size: 971 209 3825
Fragments arranged by size:
3825 971 209
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3196 3230 1903
Size: 34 1259 34 3678
Fragments arranged by size:
3678 1259 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2897 4107 2897
Size: 1210 3795
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5005
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2185 3616 136
Size: 107 122 71 631 605 513 1431 1525
Fragments arranged by size:
1525 1431 631 605 513 122 107 71
BglII A'GATC_T
Cuts at: 2062 5000 2062
Size: 2938 2067
BlpI GC'TnA_GC
Cuts at: 2422 2422
Size: 5005
Bme1580I G_kGCm'C
Cuts at: 1790 3066 4222 1790
Size: 1276 1156 2573
Fragments arranged by size:
2573 1276 1156
BmgBI CAC'GTC
Cuts at: 1318 2106 1318
Size: 788 4217
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2736 3694 442
Size: 416 53 83 399 24 1319 958 1753
Fragments arranged by size:
1753 1319 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5005
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 2183 3665 1239
Size: 794 150 1482 2579
Fragments arranged by size:
2579 1482 794 150
Bpu10I CC'TnA_GC
Cuts at: 2084 2084
Size: 5005
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3130 3995 4293 3130
Size: 865 298 3842
Fragments arranged by size:
3842 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3668 882
Size: 617 2169 2219
Fragments arranged by size:
2219 2169 617
BsaAI yAC'GTr
Cuts at: 493 1204 2796 493
Size: 711 1592 2702
Fragments arranged by size:
2702 1592 711
BsaBI GATnn'nnATC
Cuts at: 4999 4999
Size: 5005
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2812 3194 3829 275
Size: 53 83 186 936 1279 382 635 1451
Fragments arranged by size:
1451 1279 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3438 3836 4110 4257 4668 3438
Size: 398 274 147 411 3775
Fragments arranged by size:
3775 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4780
Fragments arranged by size:
4780 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5005
BseYI C'CCAG_C
Cuts at: 2282 4208 4704 2282
Size: 1926 496 2583
Fragments arranged by size:
2583 1926 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4432
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2455 3216 4244 1540
Size: 275 186 454 761 1028 2301
Fragments arranged by size:
2301 1028 761 454 275 186
BsiHKAI G_wGCw'C
Cuts at: 3066 3151 4222 3066
Size: 85 1071 3849
Fragments arranged by size:
3849 1071 85
BsmI GAATG_Cn'
Cuts at: 2516 2609 2516
Size: 93 4912
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 2324 3066 3151 4222 1391
Size: 399 493 41 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 493 399 85 41
BspEI T'CCGG_A
Cuts at: 4668 4668
Size: 5005
BspHI T'CATG_A
Cuts at: 2894 4624 2894
Size: 1730 3275
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2086 2360 844
Size: 1242 274 3489
Fragments arranged by size:
3489 1242 274
BsrBI CCG'CTC
Cuts at: 1394 1823 2892 1394
Size: 429 1069 3507
Fragments arranged by size:
3507 1069 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3500 3674 65
Size: 1183 2252 174 1396
Fragments arranged by size:
2252 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2260 3649 3836 1700
Size: 110 450 1389 187 2869
Fragments arranged by size:
2869 1389 450 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5005
BssHII G'CGCG_C
Cuts at: 1925 2239 1925
Size: 314 4691
BssSI C'ACGA_G
Cuts at: 3065 4077 3065
Size: 1012 3993
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5005
BstBI TT'CG_AA
Cuts at: 3809 3809
Size: 5005
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5005
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5005
BstYI r'GATC_y
Cuts at: 1972 2062 2335 2702 3087 3104 4556 4642 4654
Size: 90 273 367 385 17 1452 86 12
Cuts at: 4654 5000 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 273 90 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3514
Fragments arranged by size:
3514 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4266
Fragments arranged by size:
4266 683 56
BtsI GCAGTG_nn'
Cuts at: 2521 3315 3335 2521
Size: 794 20 4191
Fragments arranged by size:
4191 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5005
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4970
DraI TTT'AAA
Cuts at: 2309 2309
Size: 5005
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4012 809
Size: 507 2696 1802
Fragments arranged by size:
2696 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2452 3341 4970
Size: 54 1425 50 275 18 622 889 1629
Cuts at: 4970 8
Size: 43
Fragments arranged by size:
1629 1425 889 622 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2452 1537
Size: 275 640 4090
Fragments arranged by size:
4090 640 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2935 1353
Size: 1582 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2279 3592 3966 4112 387
Size: 1064 138 690 1313 374 146 1280
Fragments arranged by size:
1313 1280 1064 690 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2183 3068 3665 4452 4720 1239
Size: 393 401 150 885 597 787 268 1524
Fragments arranged by size:
1524 885 787 597 401 393 268 150
EcoRI G'AATT_C
Cuts at: 2314 2692 2314
Size: 378 4627
FspI TGC'GCA
Cuts at: 3511 3511
Size: 5005
HaeII r_GCGC'y
Cuts at: 1678 4152 1678
Size: 2474 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3646 3678 3720 3752 1373
Size: 32 135 32 2074 32 42 32 2626
Fragments arranged by size:
2626 2074 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2349 2595 669
Size: 988 281 411 246 3079
Fragments arranged by size:
3079 988 411 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5005
HpaI GTT'AAC
Cuts at: 2595 2595
Size: 5005
KpnI G_GTAC'C
Cuts at: 2113 2330 2113
Size: 217 4788
MfeI C'AATT_G
Cuts at: 2604 2604
Size: 5005
MluI A'CGCG_T
Cuts at: 4881 4881
Size: 5005
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2234 4119 4303 1305
Size: 929 1885 184 2007
Fragments arranged by size:
2007 1885 929 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3183
Fragments arranged by size:
3183 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2963 3322 3481 518
Size: 648 1797 359 159 2042
Fragments arranged by size:
2042 1797 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2421 3098 4246 4491 1606
Size: 815 677 1148 245 2120
Fragments arranged by size:
2120 1148 815 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3514
Fragments arranged by size:
3514 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5005
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5005
NotI GC'GGCC_GC
Cuts at: 2452 2452
Size: 5005
NspI r_CATG'y
Cuts at: 1132 1229 2363 3908 1132
Size: 97 1134 1545 2229
Fragments arranged by size:
2229 1545 1134 97
PasI CC'CwG_GG
Cuts at: 1348 2077 1348
Size: 729 4276
PciI A'CATG_T
Cuts at: 3904 3904
Size: 5005
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5005
PmeI GTTT'AAAC
Cuts at: 2309 2309
Size: 5005
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3074 3106 4587 4619 3074
Size: 32 1481 32 3460
Fragments arranged by size:
3460 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5005
PsiI TTA'TAA
Cuts at: 2575 2575
Size: 5005
PstI C_TGCA'G
Cuts at: 830 2151 2357 830
Size: 1321 206 3478
Fragments arranged by size:
3478 1321 206
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5005
SalI G'TCGA_C
Cuts at: 1936 2347 1936
Size: 411 4594
SbfI CC_TGCA'GG
Cuts at: 2357 2357
Size: 5005
ScaI AGT'ACT
Cuts at: 3253 4965 3253
Size: 1712 3293
SfcI C'TryA_G
Cuts at: 826 1045 2147 2353 3488 4169 4360 826
Size: 219 1102 206 1135 681 191 1471
Fragments arranged by size:
1471 1135 1102 681 219 206 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5005
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5005
SmaI CCC'GGG
Cuts at: 1872 2332 1872
Size: 460 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3109 4010 4272 820
Size: 197 926 1166 901 262 1553
Fragments arranged by size:
1553 1166 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2796 493
Size: 2303 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5005
SphI G_CATG'C
Cuts at: 1132 2363 1132
Size: 1231 3774
SspI AAT'ATT
Cuts at: 5 52 2929 5
Size: 47 2877 2081
Fragments arranged by size:
2877 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5005
StyI C'CwwG_G
Cuts at: 513 1065 2004 2181 2244 2444 513
Size: 552 939 177 63 200 3074
Fragments arranged by size:
3074 939 552 200 177 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3032 3049 3202 3387 3832 325
Size: 1255 1452 17 153 185 445 1498
Fragments arranged by size:
1498 1452 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3251 4963 96
Size: 274 80 33 51 1443 1274 1712 138
Fragments arranged by size:
1712 1443 1274 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4965 4997 4965
Size: 32 4973
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5005
XbaI T'CTAG_A
Cuts at: 2341 2341
Size: 5005
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5005
XmaI C'CCGG_G
Cuts at: 1870 2330 1870
Size: 460 4545
XmnI GAAnn'nnTTC
Cuts at: 2021 3134 2021
Size: 1113 3892
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2813 276
Size: 53 83 186 936 1279 2468
Fragments arranged by size:
2468 1279 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI